CA2610405A1 - Systemes de promoteurs induits par le mannitol dans des cellules hotes bacteriennes - Google Patents
Systemes de promoteurs induits par le mannitol dans des cellules hotes bacteriennes Download PDFInfo
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- CA2610405A1 CA2610405A1 CA002610405A CA2610405A CA2610405A1 CA 2610405 A1 CA2610405 A1 CA 2610405A1 CA 002610405 A CA002610405 A CA 002610405A CA 2610405 A CA2610405 A CA 2610405A CA 2610405 A1 CA2610405 A1 CA 2610405A1
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- mannitol
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- nucleic acid
- pseudomonas
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- 101150044170 trpE gene Proteins 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 229960005356 urokinase Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
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- 238000001262 western blot Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne des procédés permettant de produire des peptides recombinants dans un hôte bactérien en utilisant un promoteur inductible par le mannitol, l'arabitol, le glucitol, ou le glycérol. La cellule bactérienne hôte qui produit le peptide a été rendue incapable de dégrader ou de métaboliser le mannitol, l'arabitol, ou le glucitol, ou des dérivés ou analogues de ceux-ci. L'invention concerne des cellules bactériennes qui ont été modifiées génétiquement de manière à inhiber le métabolisme ou la dégradation du mannitol, du glucitol, ou de l'arabitol, ou des dérivés ou analogues de ceux-ci. On utilise le mannitol, l'arabitol, le glucitol, ou le glycérol pour induire l'expression d'un polypeptide cible à partir d'un promoteur inductible, ce qui permet d'utiliser un inducteur de source de carbone stable et peu coûteux dans les processus de fermentation pour la production de peptides recombinants.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US68776305P | 2005-06-06 | 2005-06-06 | |
US60/687,763 | 2005-06-06 | ||
PCT/US2006/021958 WO2006133210A2 (fr) | 2005-06-06 | 2006-06-06 | Systemes de promoteurs induits par le mannitol dans des cellules hotes bacteriennes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2610405A1 true CA2610405A1 (fr) | 2006-12-14 |
CA2610405C CA2610405C (fr) | 2015-01-27 |
Family
ID=37499050
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2610405A Active CA2610405C (fr) | 2005-06-06 | 2006-06-06 | Systemes de promoteurs induits par le mannitol dans des cellules hotes bacteriennes |
Country Status (11)
Country | Link |
---|---|
US (2) | US7476532B2 (fr) |
EP (1) | EP1888763B1 (fr) |
JP (1) | JP5176114B2 (fr) |
KR (1) | KR101304921B1 (fr) |
CN (1) | CN101193906B (fr) |
AU (1) | AU2006255060B2 (fr) |
BR (1) | BRPI0611112A2 (fr) |
CA (1) | CA2610405C (fr) |
MX (1) | MX2007015355A (fr) |
PL (1) | PL1888763T3 (fr) |
WO (1) | WO2006133210A2 (fr) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT2284273E (pt) * | 2009-08-10 | 2013-01-14 | Lonza Ag | Vetor compreendendo promotor manose e promotor manose |
EP2292769A1 (fr) * | 2009-08-10 | 2011-03-09 | Lonza AG | Processus de fermentation |
US8697398B2 (en) * | 2010-03-01 | 2014-04-15 | Dsm Ip Assets B.V. | Compositions and methods for bacterial production of chondroitin |
EA034074B1 (ru) | 2010-07-29 | 2019-12-25 | Лонца Аг | Регуляция индуцируемых промоторов |
GB201117538D0 (en) * | 2011-10-11 | 2011-11-23 | Royal Veterinary College The | Methods |
CA2880285C (fr) | 2012-08-05 | 2022-11-01 | Absci, Llc | Systeme de coexpression inductible |
US10508270B2 (en) | 2012-08-05 | 2019-12-17 | Absci Llc | Coordinated coexpression of thrombin |
US20150353940A1 (en) | 2013-08-05 | 2015-12-10 | Absci, Llc | Vectors for use in an inducible coexpression system |
JP5995218B2 (ja) * | 2012-11-08 | 2016-09-21 | 独立行政法人酒類総合研究所 | ソルビトール誘導性プロモーター |
CN103882047B (zh) * | 2014-03-18 | 2016-06-29 | 杨霞 | 重组人酸性成纤维细胞生长因子蛋白的制备方法 |
ES2952405T3 (es) | 2014-12-01 | 2023-10-31 | Pfenex Inc | Parejas de fusión para la producción de péptidos |
WO2016106504A1 (fr) * | 2014-12-29 | 2016-07-07 | 财团法人农业科技研究院 | Élément d'expression, cassette d'expression, et vecteur le ou la contenant |
CN109628533A (zh) * | 2019-01-18 | 2019-04-16 | 重庆派金生物科技有限公司 | 成纤维细胞生长因子蛋白可溶性高效重组表达的方法 |
CN111662903B (zh) * | 2019-03-08 | 2022-12-27 | 上海凯赛生物技术股份有限公司 | 对数期特异性启动子及其应用 |
SG11202110807QA (en) * | 2019-04-02 | 2021-10-28 | Spiber Inc | Method for producing recombinant protein |
CN112553235B (zh) * | 2020-12-09 | 2023-04-28 | 江南大学 | 一种甘油诱导表达系统及在纳豆激酶生产中的应用 |
EP4337774A1 (fr) * | 2021-05-12 | 2024-03-20 | The Trustees of Columbia University in the City of New York | Nanoencapsulation programmable pour l'administration de probiotiques in vivo |
CN114807204B (zh) * | 2022-04-12 | 2023-08-15 | 湖北大学 | 一种重组载体质粒、水杨酸生物传感器及构建方法和应用 |
CN115322924B (zh) * | 2022-07-19 | 2023-11-14 | 济南大学 | 一种双菌混合培养合成聚羟基脂肪酸酯的方法 |
-
2006
- 2006-06-06 AU AU2006255060A patent/AU2006255060B2/en active Active
- 2006-06-06 KR KR1020087000198A patent/KR101304921B1/ko not_active IP Right Cessation
- 2006-06-06 US US11/447,553 patent/US7476532B2/en active Active
- 2006-06-06 JP JP2008515835A patent/JP5176114B2/ja active Active
- 2006-06-06 PL PL06772320T patent/PL1888763T3/pl unknown
- 2006-06-06 MX MX2007015355A patent/MX2007015355A/es unknown
- 2006-06-06 CA CA2610405A patent/CA2610405C/fr active Active
- 2006-06-06 BR BRPI0611112-2A patent/BRPI0611112A2/pt not_active IP Right Cessation
- 2006-06-06 WO PCT/US2006/021958 patent/WO2006133210A2/fr active Application Filing
- 2006-06-06 CN CN2006800201108A patent/CN101193906B/zh active Active
- 2006-06-06 EP EP06772320.5A patent/EP1888763B1/fr active Active
-
2008
- 2008-12-09 US US12/330,723 patent/US8017355B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2006133210A2 (fr) | 2006-12-14 |
EP1888763B1 (fr) | 2015-08-12 |
AU2006255060B2 (en) | 2012-08-30 |
KR101304921B1 (ko) | 2013-09-13 |
US7476532B2 (en) | 2009-01-13 |
KR20080018937A (ko) | 2008-02-28 |
CA2610405C (fr) | 2015-01-27 |
PL1888763T3 (pl) | 2015-12-31 |
CN101193906B (zh) | 2012-09-05 |
EP1888763A4 (fr) | 2009-05-13 |
US8017355B2 (en) | 2011-09-13 |
US20060292671A1 (en) | 2006-12-28 |
BRPI0611112A2 (pt) | 2010-08-10 |
WO2006133210A3 (fr) | 2007-05-24 |
JP2008545436A (ja) | 2008-12-18 |
EP1888763A2 (fr) | 2008-02-20 |
AU2006255060A1 (en) | 2006-12-14 |
JP5176114B2 (ja) | 2013-04-03 |
US20090162899A1 (en) | 2009-06-25 |
CN101193906A (zh) | 2008-06-04 |
MX2007015355A (es) | 2008-02-19 |
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