CA2590662A1 - Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique - Google Patents
Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique Download PDFInfo
- Publication number
- CA2590662A1 CA2590662A1 CA002590662A CA2590662A CA2590662A1 CA 2590662 A1 CA2590662 A1 CA 2590662A1 CA 002590662 A CA002590662 A CA 002590662A CA 2590662 A CA2590662 A CA 2590662A CA 2590662 A1 CA2590662 A1 CA 2590662A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- sequence
- seq
- amino acid
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000186226 Corynebacterium glutamicum Species 0.000 title claims abstract description 132
- 108090000623 proteins and genes Proteins 0.000 title abstract description 387
- 102000004169 proteins and genes Human genes 0.000 title abstract description 291
- 230000037353 metabolic pathway Effects 0.000 title description 294
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 260
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 260
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 230
- 238000004519 manufacturing process Methods 0.000 claims abstract description 87
- 238000000034 method Methods 0.000 claims abstract description 74
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 51
- 239000013604 expression vector Substances 0.000 claims abstract description 45
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 45
- 235000001014 amino acid Nutrition 0.000 claims description 158
- 229940024606 amino acid Drugs 0.000 claims description 153
- 125000003729 nucleotide group Chemical group 0.000 claims description 145
- 239000002773 nucleotide Substances 0.000 claims description 142
- 150000001413 amino acids Chemical group 0.000 claims description 113
- 230000000694 effects Effects 0.000 claims description 76
- 239000013598 vector Substances 0.000 claims description 66
- 230000014509 gene expression Effects 0.000 claims description 61
- 239000012847 fine chemical Substances 0.000 claims description 56
- 102000004190 Enzymes Human genes 0.000 claims description 48
- 108090000790 Enzymes Proteins 0.000 claims description 48
- 229940088594 vitamin Drugs 0.000 claims description 46
- 229930003231 vitamin Natural products 0.000 claims description 46
- 235000013343 vitamin Nutrition 0.000 claims description 46
- 239000011782 vitamin Substances 0.000 claims description 46
- 239000002777 nucleoside Substances 0.000 claims description 40
- 229920001184 polypeptide Polymers 0.000 claims description 39
- 244000005700 microbiome Species 0.000 claims description 36
- 230000001105 regulatory effect Effects 0.000 claims description 29
- 239000012634 fragment Substances 0.000 claims description 28
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 26
- KDCGOANMDULRCW-UHFFFAOYSA-N Purine Natural products N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 25
- 150000003833 nucleoside derivatives Chemical class 0.000 claims description 25
- 241000186216 Corynebacterium Species 0.000 claims description 22
- 230000000295 complement effect Effects 0.000 claims description 20
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 18
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 17
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 17
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 16
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 16
- 239000004472 Lysine Substances 0.000 claims description 16
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 14
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 13
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims description 13
- 229960002429 proline Drugs 0.000 claims description 13
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 12
- 229960000310 isoleucine Drugs 0.000 claims description 12
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 11
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 11
- 241000186146 Brevibacterium Species 0.000 claims description 10
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 10
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 10
- 239000004473 Threonine Substances 0.000 claims description 10
- 229960005190 phenylalanine Drugs 0.000 claims description 10
- 229960002898 threonine Drugs 0.000 claims description 10
- 229960003767 alanine Drugs 0.000 claims description 9
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 9
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 8
- 239000004471 Glycine Substances 0.000 claims description 8
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 8
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 8
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 8
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 8
- 229960002885 histidine Drugs 0.000 claims description 8
- 150000002632 lipids Chemical class 0.000 claims description 8
- 229960001153 serine Drugs 0.000 claims description 8
- 239000004475 Arginine Substances 0.000 claims description 7
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 7
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 7
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 7
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 7
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 7
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 7
- 229960003121 arginine Drugs 0.000 claims description 7
- 229940009098 aspartate Drugs 0.000 claims description 7
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 7
- 235000018417 cysteine Nutrition 0.000 claims description 7
- 229960002433 cysteine Drugs 0.000 claims description 7
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 7
- 229960003136 leucine Drugs 0.000 claims description 7
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 7
- 229960004295 valine Drugs 0.000 claims description 7
- 239000004474 valine Substances 0.000 claims description 7
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 6
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 6
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 6
- 235000004279 alanine Nutrition 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 229940049906 glutamate Drugs 0.000 claims description 6
- 229930195712 glutamate Natural products 0.000 claims description 6
- 229930182817 methionine Natural products 0.000 claims description 6
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 6
- 229960004441 tyrosine Drugs 0.000 claims description 6
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 5
- 206010013023 diphtheria Diseases 0.000 claims description 5
- 150000007524 organic acids Chemical class 0.000 claims description 5
- 150000001491 aromatic compounds Chemical class 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 150000002009 diols Chemical class 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000004048 modification Effects 0.000 claims description 4
- 238000012986 modification Methods 0.000 claims description 4
- 230000004071 biological effect Effects 0.000 claims description 3
- 229930001119 polyketide Natural products 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 3
- IGFXRKMLLMBKSA-UHFFFAOYSA-N purine Chemical compound N1=C[N]C2=NC=NC2=C1 IGFXRKMLLMBKSA-UHFFFAOYSA-N 0.000 claims description 3
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 150000004671 saturated fatty acids Chemical class 0.000 claims description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 claims description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 claims description 3
- 241000186145 Corynebacterium ammoniagenes Species 0.000 claims 2
- 241000187561 Rhodococcus erythropolis Species 0.000 claims 2
- 150000003881 polyketide derivatives Chemical class 0.000 claims 2
- 235000012539 Bacterium linens Nutrition 0.000 claims 1
- 241000186310 Brevibacterium linens Species 0.000 claims 1
- 241001517047 Corynebacterium acetoacidophilum Species 0.000 claims 1
- 241000319304 [Brevibacterium] flavum Species 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 50
- 230000000692 anti-sense effect Effects 0.000 abstract description 38
- 102000037865 fusion proteins Human genes 0.000 abstract description 25
- 108020001507 fusion proteins Proteins 0.000 abstract description 25
- 238000003259 recombinant expression Methods 0.000 abstract description 14
- 238000010353 genetic engineering Methods 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 abstract description 3
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 282
- 210000004027 cell Anatomy 0.000 description 127
- 125000003275 alpha amino acid group Chemical group 0.000 description 76
- 230000006870 function Effects 0.000 description 72
- 108020004414 DNA Proteins 0.000 description 68
- -1 cofactors Substances 0.000 description 66
- 230000015572 biosynthetic process Effects 0.000 description 55
- 230000004060 metabolic process Effects 0.000 description 48
- 229940088598 enzyme Drugs 0.000 description 47
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 40
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 40
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 38
- 235000021436 nutraceutical agent Nutrition 0.000 description 35
- 239000002417 nutraceutical Substances 0.000 description 34
- 101150031278 MP gene Proteins 0.000 description 32
- 108091028043 Nucleic acid sequence Proteins 0.000 description 31
- 239000000543 intermediate Substances 0.000 description 25
- 241000894006 Bacteria Species 0.000 description 23
- 239000000126 substance Substances 0.000 description 23
- 108091026890 Coding region Proteins 0.000 description 22
- 108010064711 Homoserine dehydrogenase Proteins 0.000 description 22
- 230000001965 increasing effect Effects 0.000 description 22
- 239000002243 precursor Substances 0.000 description 22
- 102000016397 Methyltransferase Human genes 0.000 description 21
- 108060004795 Methyltransferase Proteins 0.000 description 21
- 238000003786 synthesis reaction Methods 0.000 description 21
- 230000037361 pathway Effects 0.000 description 20
- 108700036577 EC 1.4.1.13 Proteins 0.000 description 19
- 230000001851 biosynthetic effect Effects 0.000 description 18
- 230000003413 degradative effect Effects 0.000 description 18
- 108010002610 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase Proteins 0.000 description 17
- 108010055400 Aspartate kinase Proteins 0.000 description 17
- 125000000539 amino acid group Chemical group 0.000 description 17
- 238000010367 cloning Methods 0.000 description 17
- 102100039654 Phosphoribosylglycinamide formyltransferase Human genes 0.000 description 16
- 108010064209 Phosphoribosylglycinamide formyltransferase Proteins 0.000 description 16
- 230000000875 corresponding effect Effects 0.000 description 16
- 108020004999 messenger RNA Proteins 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- 102000003960 Ligases Human genes 0.000 description 15
- 108090000364 Ligases Proteins 0.000 description 15
- 238000006911 enzymatic reaction Methods 0.000 description 15
- 125000003835 nucleoside group Chemical group 0.000 description 15
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 14
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 14
- 102100034193 Aspartate aminotransferase, mitochondrial Human genes 0.000 description 14
- 108030002081 Aspartate transaminases Proteins 0.000 description 14
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 14
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 14
- 235000018977 lysine Nutrition 0.000 description 14
- 230000035772 mutation Effects 0.000 description 14
- 230000014616 translation Effects 0.000 description 14
- 108010022394 Threonine synthase Proteins 0.000 description 13
- 230000001413 cellular effect Effects 0.000 description 13
- 230000004927 fusion Effects 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 12
- 108010080611 Cytosine Deaminase Proteins 0.000 description 12
- 102000000311 Cytosine Deaminase Human genes 0.000 description 12
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 11
- 101710165738 Acetylornithine aminotransferase Proteins 0.000 description 11
- 108010049445 Acetylornithine transaminase Proteins 0.000 description 11
- 101710088194 Dehydrogenase Proteins 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 11
- 229960001570 ademetionine Drugs 0.000 description 11
- 230000015556 catabolic process Effects 0.000 description 11
- 238000009396 hybridization Methods 0.000 description 11
- 235000019161 pantothenic acid Nutrition 0.000 description 11
- 239000011713 pantothenic acid Substances 0.000 description 11
- 241000186227 Corynebacterium diphtheriae Species 0.000 description 10
- 102000053602 DNA Human genes 0.000 description 10
- 108091034117 Oligonucleotide Proteins 0.000 description 10
- 102100021762 Phosphoserine phosphatase Human genes 0.000 description 10
- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 description 10
- 239000002253 acid Substances 0.000 description 10
- 238000012512 characterization method Methods 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000006731 degradation reaction Methods 0.000 description 10
- 238000012217 deletion Methods 0.000 description 10
- 230000037430 deletion Effects 0.000 description 10
- 238000002703 mutagenesis Methods 0.000 description 10
- 231100000350 mutagenesis Toxicity 0.000 description 10
- 229940014662 pantothenate Drugs 0.000 description 10
- 108010076573 phosphoserine phosphatase Proteins 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 10
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 10
- 108091000126 Dihydroorotase Proteins 0.000 description 9
- 241000233866 Fungi Species 0.000 description 9
- 102100034013 Gamma-glutamyl phosphate reductase Human genes 0.000 description 9
- 108010063678 Indole-3-Glycerol-Phosphate Synthase Proteins 0.000 description 9
- 108091005461 Nucleic proteins Proteins 0.000 description 9
- 108030005835 Phosphooxymethylpyrimidine kinases Proteins 0.000 description 9
- 102100027330 Phosphoribosylaminoimidazole carboxylase Human genes 0.000 description 9
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 9
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 9
- 108010060059 Sarcosine Oxidase Proteins 0.000 description 9
- 102000008118 Sarcosine oxidase Human genes 0.000 description 9
- 239000012707 chemical precursor Substances 0.000 description 9
- 239000000306 component Substances 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 9
- 230000004151 fermentation Effects 0.000 description 9
- 230000002068 genetic effect Effects 0.000 description 9
- 238000002744 homologous recombination Methods 0.000 description 9
- 230000006801 homologous recombination Effects 0.000 description 9
- 238000010369 molecular cloning Methods 0.000 description 9
- 108010006451 phosphomethylpyrimidine kinase Proteins 0.000 description 9
- 108010034343 phosphoribosylamine-glycine ligase Proteins 0.000 description 9
- 238000006467 substitution reaction Methods 0.000 description 9
- 235000008521 threonine Nutrition 0.000 description 9
- 108010066009 AMP nucleosidase Proteins 0.000 description 8
- 108010037870 Anthranilate Synthase Proteins 0.000 description 8
- 102000009042 Argininosuccinate Lyase Human genes 0.000 description 8
- 102100034581 Dihydroorotase Human genes 0.000 description 8
- 108700016256 Dihydropteroate synthases Proteins 0.000 description 8
- 108010020869 Homocysteine S-Methyltransferase Proteins 0.000 description 8
- 102000004157 Hydrolases Human genes 0.000 description 8
- 108090000604 Hydrolases Proteins 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 8
- 230000004075 alteration Effects 0.000 description 8
- 230000002255 enzymatic effect Effects 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 8
- 108010035774 phosphoribosylaminoimidazole carboxylase Proteins 0.000 description 8
- 239000013615 primer Substances 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 238000013518 transcription Methods 0.000 description 8
- 230000035897 transcription Effects 0.000 description 8
- 102100025981 Aminoacylase-1 Human genes 0.000 description 7
- 108090000994 Catalytic RNA Proteins 0.000 description 7
- 102000053642 Catalytic RNA Human genes 0.000 description 7
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 7
- 108700034048 EC 4.2.99.8 Proteins 0.000 description 7
- 108010000200 Ketol-acid reductoisomerase Proteins 0.000 description 7
- 102000011882 N-acyl-L-amino-acid amidohydrolases Human genes 0.000 description 7
- 108050002335 N-acyl-L-amino-acid amidohydrolases Proteins 0.000 description 7
- 108030006755 N-acyl-aliphatic-L-amino acid amidohydrolases Proteins 0.000 description 7
- 229910019142 PO4 Inorganic materials 0.000 description 7
- 102000003929 Transaminases Human genes 0.000 description 7
- 108090000340 Transaminases Proteins 0.000 description 7
- 230000003321 amplification Effects 0.000 description 7
- 229960004452 methionine Drugs 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 239000010452 phosphate Substances 0.000 description 7
- 230000004952 protein activity Effects 0.000 description 7
- 230000004144 purine metabolism Effects 0.000 description 7
- 150000003212 purines Chemical class 0.000 description 7
- 235000019192 riboflavin Nutrition 0.000 description 7
- 229960002477 riboflavin Drugs 0.000 description 7
- 239000002151 riboflavin Substances 0.000 description 7
- 108091000042 riboflavin kinase Proteins 0.000 description 7
- 108091092562 ribozyme Proteins 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 7
- 230000009466 transformation Effects 0.000 description 7
- 238000013519 translation Methods 0.000 description 7
- 108091000036 uracil phosphoribosyltransferase Proteins 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- RQFCJASXJCIDSX-UHFFFAOYSA-N 14C-Guanosin-5'-monophosphat Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(O)=O)C(O)C1O RQFCJASXJCIDSX-UHFFFAOYSA-N 0.000 description 6
- 108010065780 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Proteins 0.000 description 6
- 102000005234 Adenosylhomocysteinase Human genes 0.000 description 6
- 108020002202 Adenosylhomocysteinase Proteins 0.000 description 6
- 108090000164 Carbamoyl-phosphate synthase (glutamine-hydrolyzing) Proteins 0.000 description 6
- 108700035543 EC 4.2.99.10 Proteins 0.000 description 6
- 108010016106 Glutamate-5-semialdehyde dehydrogenase Proteins 0.000 description 6
- XKMLYUALXHKNFT-UUOKFMHZSA-N Guanosine-5'-triphosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XKMLYUALXHKNFT-UUOKFMHZSA-N 0.000 description 6
- 108030002088 Histidinol-phosphate transaminases Proteins 0.000 description 6
- 101710083973 Homocysteine synthase Proteins 0.000 description 6
- 102000006933 Hydroxymethyl and Formyl Transferases Human genes 0.000 description 6
- 108010072462 Hydroxymethyl and Formyl Transferases Proteins 0.000 description 6
- 108010090758 L-gulonolactone oxidase Proteins 0.000 description 6
- 108030006431 Methionine synthases Proteins 0.000 description 6
- 102000004316 Oxidoreductases Human genes 0.000 description 6
- 108090000854 Oxidoreductases Proteins 0.000 description 6
- 102000002681 Polyribonucleotide nucleotidyltransferase Human genes 0.000 description 6
- 108010015724 Prephenate Dehydratase Proteins 0.000 description 6
- 108010035004 Prephenate Dehydrogenase Proteins 0.000 description 6
- 108020001991 Protoporphyrinogen Oxidase Proteins 0.000 description 6
- 102000005135 Protoporphyrinogen oxidase Human genes 0.000 description 6
- 108010070648 Pyridoxal Kinase Proteins 0.000 description 6
- 108020004511 Recombinant DNA Proteins 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 235000009697 arginine Nutrition 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 102000004419 dihydrofolate reductase Human genes 0.000 description 6
- 210000003527 eukaryotic cell Anatomy 0.000 description 6
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 description 6
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 108010085336 phosphoribosyl-AMP cyclohydrolase Proteins 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 210000001236 prokaryotic cell Anatomy 0.000 description 6
- 238000001243 protein synthesis Methods 0.000 description 6
- XKMLYUALXHKNFT-UHFFFAOYSA-N rGTP Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O XKMLYUALXHKNFT-UHFFFAOYSA-N 0.000 description 6
- 238000012552 review Methods 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 238000011144 upstream manufacturing Methods 0.000 description 6
- GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 5
- 108010039224 Amidophosphoribosyltransferase Proteins 0.000 description 5
- 101710191958 Amino-acid acetyltransferase Proteins 0.000 description 5
- 108020001077 Anthranilate Phosphoribosyltransferase Proteins 0.000 description 5
- 108090000489 Carboxy-Lyases Proteins 0.000 description 5
- 102000004031 Carboxy-Lyases Human genes 0.000 description 5
- 108091033380 Coding strand Proteins 0.000 description 5
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 5
- 101710109085 Cysteine synthase, chloroplastic/chromoplastic Proteins 0.000 description 5
- 108030003594 Diaminopimelate decarboxylases Proteins 0.000 description 5
- 108700034049 EC 4.2.99.9 Proteins 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 101710198928 Gamma-glutamyl phosphate reductase Proteins 0.000 description 5
- 102000005720 Glutathione transferase Human genes 0.000 description 5
- 108010070675 Glutathione transferase Proteins 0.000 description 5
- 101710115197 Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase Proteins 0.000 description 5
- 102100026823 Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 Human genes 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 5
- 108050003783 Histidinol-phosphate aminotransferase Proteins 0.000 description 5
- GRSZFWQUAKGDAV-KQYNXXCUSA-N IMP Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-KQYNXXCUSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 5
- 101710138316 O-acetylserine sulfhydrylase Proteins 0.000 description 5
- 108010061618 O-succinylhomoserine (thiol)-lyase Proteins 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 108091000041 Phosphoenolpyruvate Carboxylase Proteins 0.000 description 5
- 108010038555 Phosphoglycerate dehydrogenase Proteins 0.000 description 5
- 102100036473 Phosphoribosylformylglycinamidine synthase Human genes 0.000 description 5
- 108030004873 Phosphoribosylformylglycinamidine synthases Proteins 0.000 description 5
- 108091000080 Phosphotransferase Proteins 0.000 description 5
- 238000012300 Sequence Analysis Methods 0.000 description 5
- 108010006873 Threonine Dehydratase Proteins 0.000 description 5
- 108010075344 Tryptophan synthase Proteins 0.000 description 5
- 108020000963 Uroporphyrinogen-III synthase Proteins 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 5
- 229950006790 adenosine phosphate Drugs 0.000 description 5
- 239000002585 base Substances 0.000 description 5
- 239000002537 cosmetic Substances 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 229960002989 glutamic acid Drugs 0.000 description 5
- 102000005396 glutamine synthetase Human genes 0.000 description 5
- 108020002326 glutamine synthetase Proteins 0.000 description 5
- 108010034653 homoserine O-acetyltransferase Proteins 0.000 description 5
- 210000004962 mammalian cell Anatomy 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 235000006109 methionine Nutrition 0.000 description 5
- 108010046778 molybdenum cofactor Proteins 0.000 description 5
- HPEUEJRPDGMIMY-IFQPEPLCSA-N molybdopterin Chemical compound O([C@H]1N2)[C@H](COP(O)(O)=O)C(S)=C(S)[C@@H]1NC1=C2N=C(N)NC1=O HPEUEJRPDGMIMY-IFQPEPLCSA-N 0.000 description 5
- 229950006238 nadide Drugs 0.000 description 5
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 5
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 101150044170 trpE gene Proteins 0.000 description 5
- 102000003643 uroporphyrinogen-III synthase Human genes 0.000 description 5
- 230000003612 virological effect Effects 0.000 description 5
- 229940011671 vitamin b6 Drugs 0.000 description 5
- 108050006012 1,4-dihydroxy-2-naphthoate octaprenyltransferases Proteins 0.000 description 4
- 108010021680 2-oxoglutarate decarboxylase Proteins 0.000 description 4
- 108010038550 3-dehydroquinate dehydratase Proteins 0.000 description 4
- 108010039636 3-isopropylmalate dehydrogenase Proteins 0.000 description 4
- 108010036575 3-methyl-2-oxobutanoate hydroxymethyltransferase Proteins 0.000 description 4
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 4
- 101710131710 5-amino-6-(5-phosphoribosylamino)uracil reductase Proteins 0.000 description 4
- 102000007433 5-formyltetrahydrofolate cyclo-ligase Human genes 0.000 description 4
- 102000055161 Adenylosuccinate lyases Human genes 0.000 description 4
- 102100039239 Amidophosphoribosyltransferase Human genes 0.000 description 4
- 102000053640 Argininosuccinate synthases Human genes 0.000 description 4
- 108700024106 Argininosuccinate synthases Proteins 0.000 description 4
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 4
- 108700016171 Aspartate ammonia-lyases Proteins 0.000 description 4
- 102100024265 Beta-ureidopropionase Human genes 0.000 description 4
- 108090000732 Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) Proteins 0.000 description 4
- 102100036461 Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] Human genes 0.000 description 4
- 108010088278 Branched-chain-amino-acid transaminase Proteins 0.000 description 4
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical group O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 4
- 102100037579 D-3-phosphoglycerate dehydrogenase Human genes 0.000 description 4
- 239000003155 DNA primer Substances 0.000 description 4
- 101710143817 Diaminohydroxyphosphoribosylaminopyrimidine deaminase Proteins 0.000 description 4
- 108010014468 Dihydrodipicolinate Reductase Proteins 0.000 description 4
- 108700034993 EC 1.1.1.86 Proteins 0.000 description 4
- 108700035109 EC 2.4.2.11 Proteins 0.000 description 4
- 108700035983 EC 4.3.1.8 Proteins 0.000 description 4
- 108010057394 Ferrochelatase Proteins 0.000 description 4
- 102100025413 Formyltetrahydrofolate synthetase Human genes 0.000 description 4
- 108010021555 GTP Pyrophosphokinase Proteins 0.000 description 4
- 102000005133 Glutamate 5-kinase Human genes 0.000 description 4
- 108010073324 Glutaminase Proteins 0.000 description 4
- 102000009127 Glutaminase Human genes 0.000 description 4
- 108010043428 Glycine hydroxymethyltransferase Proteins 0.000 description 4
- 102000002667 Glycine hydroxymethyltransferase Human genes 0.000 description 4
- 108010003774 Histidinol-phosphatase Proteins 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 108010056651 Hydroxymethylbilane synthase Proteins 0.000 description 4
- 108010087227 IMP Dehydrogenase Proteins 0.000 description 4
- 101710184915 Inactive 5-amino-6-(5-phosphoribosylamino)uracil reductase Proteins 0.000 description 4
- 102100021602 Inosine-5'-monophosphate dehydrogenase 1 Human genes 0.000 description 4
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 4
- 108010010685 Methenyltetrahydrofolate cyclohydrolase Proteins 0.000 description 4
- 102000000780 Nicotinate phosphoribosyltransferase Human genes 0.000 description 4
- 108700040046 Nicotinate phosphoribosyltransferases Proteins 0.000 description 4
- 102000013901 Nucleoside diphosphate kinase Human genes 0.000 description 4
- 108010083499 Ornithine cyclodeaminase Proteins 0.000 description 4
- 108010055012 Orotidine-5'-phosphate decarboxylase Proteins 0.000 description 4
- 102100034391 Porphobilinogen deaminase Human genes 0.000 description 4
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- 101100408135 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) phnA gene Proteins 0.000 description 4
- 101710170350 Riboflavin biosynthesis intermediates N-glycosidase Proteins 0.000 description 4
- 102000048125 Riboflavin kinases Human genes 0.000 description 4
- 102000028649 Ribonucleoside-diphosphate reductase Human genes 0.000 description 4
- 108050008280 Shikimate dehydrogenase Proteins 0.000 description 4
- 108010051753 Spermidine Synthase Proteins 0.000 description 4
- 102100030413 Spermidine synthase Human genes 0.000 description 4
- 102000006843 Threonine synthase Human genes 0.000 description 4
- 108030006858 Uroporphyrinogen-III C-methyltransferases Proteins 0.000 description 4
- 229930003779 Vitamin B12 Natural products 0.000 description 4
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 4
- 229960001230 asparagine Drugs 0.000 description 4
- 235000009582 asparagine Nutrition 0.000 description 4
- 108010036968 beta-ureidopropionase Proteins 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 4
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 4
- 239000005516 coenzyme A Substances 0.000 description 4
- 229940093530 coenzyme a Drugs 0.000 description 4
- 239000003184 complementary RNA Substances 0.000 description 4
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 4
- 108010089355 dihydroneopterin aldolase Proteins 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000019152 folic acid Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 238000013507 mapping Methods 0.000 description 4
- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 4
- 235000001968 nicotinic acid Nutrition 0.000 description 4
- 239000011664 nicotinic acid Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 108020003551 pantothenate synthetase Proteins 0.000 description 4
- 102000020233 phosphotransferase Human genes 0.000 description 4
- 239000002987 primer (paints) Substances 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000004147 pyrimidine metabolism Effects 0.000 description 4
- 108700022487 rRNA Genes Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 108010073086 succinyl-CoA-tetrahydrodipicolinate N-succinyltransferase Proteins 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 108010020589 trehalose-6-phosphate synthase Proteins 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 235000019163 vitamin B12 Nutrition 0.000 description 4
- 239000011715 vitamin B12 Substances 0.000 description 4
- OTOIIPJYVQJATP-BYPYZUCNSA-N (R)-pantoic acid Chemical compound OCC(C)(C)[C@@H](O)C(O)=O OTOIIPJYVQJATP-BYPYZUCNSA-N 0.000 description 3
- UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 3
- PJODNTCLCCRNCO-UHFFFAOYSA-N 2,2-diaminononanoic acid Chemical compound CCCCCCCC(N)(N)C(O)=O PJODNTCLCCRNCO-UHFFFAOYSA-N 0.000 description 3
- 108030002750 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferases Proteins 0.000 description 3
- RXMWXENJQAINCC-DMTCNVIQSA-N 2,5-didehydro-D-gluconic acid Chemical compound OCC(=O)[C@@H](O)[C@H](O)C(=O)C(O)=O RXMWXENJQAINCC-DMTCNVIQSA-N 0.000 description 3
- RXMWXENJQAINCC-UHFFFAOYSA-N 2,5-diketo-D-gluconic acid Natural products OCC(=O)C(O)C(O)C(=O)C(O)=O RXMWXENJQAINCC-UHFFFAOYSA-N 0.000 description 3
- 108030006388 3-demethylubiquinol 3-O-methyltransferases Proteins 0.000 description 3
- 108010028984 3-isopropylmalate dehydratase Proteins 0.000 description 3
- 108010058756 ATP phosphoribosyltransferase Proteins 0.000 description 3
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 3
- 108010041525 Alanine racemase Proteins 0.000 description 3
- 102000006589 Alpha-ketoglutarate dehydrogenase Human genes 0.000 description 3
- 108020004306 Alpha-ketoglutarate dehydrogenase Proteins 0.000 description 3
- 101710087198 Anthranilate synthase component II Proteins 0.000 description 3
- 108020005544 Antisense RNA Proteins 0.000 description 3
- 108700040066 Argininosuccinate lyases Proteins 0.000 description 3
- 101710117026 Biotin synthase Proteins 0.000 description 3
- 102100026413 Branched-chain-amino-acid aminotransferase, mitochondrial Human genes 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 108010003662 Chorismate synthase Proteins 0.000 description 3
- 241000186031 Corynebacteriaceae Species 0.000 description 3
- PCDQPRRSZKQHHS-UHFFFAOYSA-N Cytidine 5'-triphosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-UHFFFAOYSA-N 0.000 description 3
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 3
- 230000006820 DNA synthesis Effects 0.000 description 3
- WQXNXVUDBPYKBA-UHFFFAOYSA-N Ectoine Natural products CC1=NCCC(C(O)=O)N1 WQXNXVUDBPYKBA-UHFFFAOYSA-N 0.000 description 3
- 102100031780 Endonuclease Human genes 0.000 description 3
- 102100034545 FAD synthase region Human genes 0.000 description 3
- 108030006765 Formyltetrahydrofolate deformylases Proteins 0.000 description 3
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 3
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 3
- 108010023555 GTP Cyclohydrolase Proteins 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
- 101000594820 Homo sapiens Purine nucleoside phosphorylase Proteins 0.000 description 3
- 108010052919 Hydroxyethylthiazole kinase Proteins 0.000 description 3
- 108010007666 IMP cyclohydrolase Proteins 0.000 description 3
- 101150005343 INHA gene Proteins 0.000 description 3
- 102100020796 Inosine 5'-monophosphate cyclohydrolase Human genes 0.000 description 3
- 102000016600 Inosine-5'-monophosphate dehydrogenases Human genes 0.000 description 3
- 108050006182 Inosine-5'-monophosphate dehydrogenases Proteins 0.000 description 3
- 102000004195 Isomerases Human genes 0.000 description 3
- 108090000769 Isomerases Proteins 0.000 description 3
- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 description 3
- UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 3
- 229930182844 L-isoleucine Natural products 0.000 description 3
- 102000004317 Lyases Human genes 0.000 description 3
- 108090000856 Lyases Proteins 0.000 description 3
- 108010072610 N-acetyl-gamma-glutamyl-phosphate reductase Proteins 0.000 description 3
- 108091092724 Noncoding DNA Proteins 0.000 description 3
- 102000007981 Ornithine carbamoyltransferase Human genes 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 3
- 108700016154 Polyribonucleotide nucleotidyltransferases Proteins 0.000 description 3
- 108090000612 Proline Oxidase Proteins 0.000 description 3
- 102000004177 Proline oxidase Human genes 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- 108010086211 Riboflavin synthase Proteins 0.000 description 3
- 108020000772 Ribose-Phosphate Pyrophosphokinase Proteins 0.000 description 3
- 101100245038 Synechocystis sp. (strain PCC 6803 / Kazusa) proA1 gene Proteins 0.000 description 3
- 102000005497 Thymidylate Synthase Human genes 0.000 description 3
- 101710187777 Tryptophan synthase beta chain Proteins 0.000 description 3
- DJJCXFVJDGTHFX-UHFFFAOYSA-N Uridinemonophosphate Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-UHFFFAOYSA-N 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 239000005557 antagonist Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 239000000074 antisense oligonucleotide Substances 0.000 description 3
- 238000012230 antisense oligonucleotides Methods 0.000 description 3
- 101150040872 aroE gene Proteins 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 230000006652 catabolic pathway Effects 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 239000005515 coenzyme Substances 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000009833 condensation Methods 0.000 description 3
- 230000005494 condensation Effects 0.000 description 3
- PCDQPRRSZKQHHS-ZAKLUEHWSA-N cytidine-5'-triphosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO[P@](O)(=O)O[P@@](O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-ZAKLUEHWSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- WQXNXVUDBPYKBA-YFKPBYRVSA-N ectoine Chemical compound CC1=[NH+][C@H](C([O-])=O)CCN1 WQXNXVUDBPYKBA-YFKPBYRVSA-N 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 3
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 3
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 3
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 description 3
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 3
- 150000002224 folic acids Chemical class 0.000 description 3
- 235000013922 glutamic acid Nutrition 0.000 description 3
- 239000004220 glutamic acid Substances 0.000 description 3
- 229960002743 glutamine Drugs 0.000 description 3
- 230000034659 glycolysis Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 101150087199 leuA gene Proteins 0.000 description 3
- 101150033534 lysA gene Proteins 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000007479 molecular analysis Methods 0.000 description 3
- 101150034514 murC gene Proteins 0.000 description 3
- 229960003512 nicotinic acid Drugs 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 3
- DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 description 3
- 108010028025 phosphoribosyl-ATP pyrophosphatase Proteins 0.000 description 3
- 101150118057 proA gene Proteins 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 108020001482 shikimate kinase Proteins 0.000 description 3
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 description 3
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 description 3
- 238000012916 structural analysis Methods 0.000 description 3
- 235000019157 thiamine Nutrition 0.000 description 3
- 229960003495 thiamine Drugs 0.000 description 3
- 239000011721 thiamine Substances 0.000 description 3
- 229960004072 thrombin Drugs 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 101150108727 trpl gene Proteins 0.000 description 3
- 241000701447 unidentified baculovirus Species 0.000 description 3
- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 description 3
- 235000019158 vitamin B6 Nutrition 0.000 description 3
- 239000011726 vitamin B6 Substances 0.000 description 3
- 108030001162 1,4-dihydroxy-2-naphthoyl-CoA synthases Proteins 0.000 description 2
- 102100025573 1-alkyl-2-acetylglycerophosphocholine esterase Human genes 0.000 description 2
- RFLVMTUMFYRZCB-UHFFFAOYSA-N 1-methylguanine Chemical compound O=C1N(C)C(N)=NC2=C1N=CN2 RFLVMTUMFYRZCB-UHFFFAOYSA-N 0.000 description 2
- 108010068646 2',3'-cyclic nucleotide 2'-phosphodiesterase Proteins 0.000 description 2
- YSAJFXWTVFGPAX-UHFFFAOYSA-N 2-[(2,4-dioxo-1h-pyrimidin-5-yl)oxy]acetic acid Chemical compound OC(=O)COC1=CNC(=O)NC1=O YSAJFXWTVFGPAX-UHFFFAOYSA-N 0.000 description 2
- FZWGECJQACGGTI-UHFFFAOYSA-N 2-amino-7-methyl-1,7-dihydro-6H-purin-6-one Chemical compound NC1=NC(O)=C2N(C)C=NC2=N1 FZWGECJQACGGTI-UHFFFAOYSA-N 0.000 description 2
- 108010070495 2-dehydropantoate 2-reductase Proteins 0.000 description 2
- 108010048295 2-isopropylmalate synthase Proteins 0.000 description 2
- 108010030844 2-methylcitrate synthase Proteins 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 2
- 108010018496 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase Proteins 0.000 description 2
- BYIRZDTVVMWWFI-UHFFFAOYSA-N 2h-pteridin-1-ylmethanol Chemical compound C1=CN=C2N(CO)CN=CC2=N1 BYIRZDTVVMWWFI-UHFFFAOYSA-N 0.000 description 2
- 108010046716 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Proteins 0.000 description 2
- 102100035923 4-aminobutyrate aminotransferase, mitochondrial Human genes 0.000 description 2
- 108091022918 4-carboxymuconolactone decarboxylase Proteins 0.000 description 2
- 108091000044 4-hydroxy-tetrahydrodipicolinate synthase Proteins 0.000 description 2
- OVONXEQGWXGFJD-UHFFFAOYSA-N 4-sulfanylidene-1h-pyrimidin-2-one Chemical compound SC=1C=CNC(=O)N=1 OVONXEQGWXGFJD-UHFFFAOYSA-N 0.000 description 2
- OIVLITBTBDPEFK-UHFFFAOYSA-N 5,6-dihydrouracil Chemical compound O=C1CCNC(=O)N1 OIVLITBTBDPEFK-UHFFFAOYSA-N 0.000 description 2
- 108030006956 5-amino-6-(5-phosphoribosylamino)uracil reductases Proteins 0.000 description 2
- 108020005541 5-formyltetrahydrofolate cyclo-ligase Proteins 0.000 description 2
- ZLAQATDNGLKIEV-UHFFFAOYSA-N 5-methyl-2-sulfanylidene-1h-pyrimidin-4-one Chemical compound CC1=CNC(=S)NC1=O ZLAQATDNGLKIEV-UHFFFAOYSA-N 0.000 description 2
- HUNCSWANZMJLPM-UHFFFAOYSA-N 5-methyltryptophan Chemical compound CC1=CC=C2NC=C(CC(N)C(O)=O)C2=C1 HUNCSWANZMJLPM-UHFFFAOYSA-N 0.000 description 2
- 108010068996 6,7-dimethyl-8-ribityllumazine synthase Proteins 0.000 description 2
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 2
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 2
- 108010042833 7,8-diaminopelargonic acid aminotransferase Proteins 0.000 description 2
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 108010000700 Acetolactate synthase Proteins 0.000 description 2
- 101800001241 Acetylglutamate kinase Proteins 0.000 description 2
- 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 description 2
- 102100029457 Adenine phosphoribosyltransferase Human genes 0.000 description 2
- 102000002281 Adenylate kinase Human genes 0.000 description 2
- 108020000543 Adenylate kinase Proteins 0.000 description 2
- 108700040193 Adenylosuccinate lyases Proteins 0.000 description 2
- 108010056443 Adenylosuccinate synthase Proteins 0.000 description 2
- 108010032178 Amino-acid N-acetyltransferase Proteins 0.000 description 2
- 102000007610 Amino-acid N-acetyltransferase Human genes 0.000 description 2
- 108010073634 Aminodeoxychorismate lyase Proteins 0.000 description 2
- 101710180638 Aminodeoxychorismate synthase, chloroplastic Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 101000614853 Arabidopsis thaliana 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal Proteins 0.000 description 2
- 101710149879 Arginine repressor Proteins 0.000 description 2
- 108010014885 Arginine-tRNA ligase Proteins 0.000 description 2
- 102100036131 Arginine-tRNA ligase, cytoplasmic Human genes 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 108010024976 Asparaginase Proteins 0.000 description 2
- 102100023927 Asparagine synthetase [glutamine-hydrolyzing] Human genes 0.000 description 2
- 108030003579 Aspartate 1-decarboxylases Proteins 0.000 description 2
- 108020004652 Aspartate-Semialdehyde Dehydrogenase Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 2
- 101100350224 Bacillus subtilis (strain 168) pdhB gene Proteins 0.000 description 2
- 101100096227 Bacteroides fragilis (strain 638R) argF' gene Proteins 0.000 description 2
- 108700040198 Biotin synthases Proteins 0.000 description 2
- 101710105123 Carbamoyl-phosphate synthase large chain Proteins 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 108010000898 Chorismate mutase Proteins 0.000 description 2
- 108010071536 Citrate (Si)-synthase Proteins 0.000 description 2
- 102000006732 Citrate synthase Human genes 0.000 description 2
- 101100163308 Clostridium perfringens (strain 13 / Type A) argR1 gene Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 101100236536 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) glcB gene Proteins 0.000 description 2
- 241001485655 Corynebacterium glutamicum ATCC 13032 Species 0.000 description 2
- 241000918600 Corynebacterium ulcerans Species 0.000 description 2
- 108030003307 Creatinine deaminases Proteins 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- 102100026278 Cysteine sulfinic acid decarboxylase Human genes 0.000 description 2
- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 description 2
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 2
- 101710096438 DNA-binding protein Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 108030003318 Diaminohydroxyphosphoribosylaminopyrimidine deaminases Proteins 0.000 description 2
- 108010001625 Diaminopimelate epimerase Proteins 0.000 description 2
- 102000028526 Dihydrolipoamide Dehydrogenase Human genes 0.000 description 2
- 108010028127 Dihydrolipoamide Dehydrogenase Proteins 0.000 description 2
- 102100023319 Dihydrolipoyl dehydrogenase, mitochondrial Human genes 0.000 description 2
- 108010028196 Dihydropteridine Reductase Proteins 0.000 description 2
- 102100022317 Dihydropteridine reductase Human genes 0.000 description 2
- 108700035164 EC 4.2.1.13 Proteins 0.000 description 2
- 108700035100 EC 4.2.1.52 Proteins 0.000 description 2
- 102000003875 Ferrochelatase Human genes 0.000 description 2
- 102100030771 Ferrochelatase, mitochondrial Human genes 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 2
- 102100035067 Folylpolyglutamate synthase, mitochondrial Human genes 0.000 description 2
- 101150099894 GDHA gene Proteins 0.000 description 2
- 102100033452 GMP synthase [glutamine-hydrolyzing] Human genes 0.000 description 2
- 102000036509 GTP Cyclohydrolase Human genes 0.000 description 2
- 102100027346 GTP cyclohydrolase 1 Human genes 0.000 description 2
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 2
- 108700023479 Glutamate 5-kinases Proteins 0.000 description 2
- 108010052375 Glutamate Dehydrogenase (NADP+) Proteins 0.000 description 2
- 108030000852 Glutamate N-acetyltransferases Proteins 0.000 description 2
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 2
- 108010064766 Glutamate formimidoyltransferase Proteins 0.000 description 2
- 108010057891 Glutamate-1-semialdehyde 2,1-aminomutase Proteins 0.000 description 2
- 108020004202 Guanylate Kinase Proteins 0.000 description 2
- 101100177551 Homo sapiens HEMK1 gene Proteins 0.000 description 2
- 101001131930 Homo sapiens Transcriptional activator protein Pur-beta Proteins 0.000 description 2
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 108020003285 Isocitrate lyase Proteins 0.000 description 2
- ODBLHEXUDAPZAU-FONMRSAGSA-N Isocitric acid Natural products OC(=O)[C@@H](O)[C@H](C(O)=O)CC(O)=O ODBLHEXUDAPZAU-FONMRSAGSA-N 0.000 description 2
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 2
- 229930195722 L-methionine Natural products 0.000 description 2
- 102100022211 MTRF1L release factor glutamine methyltransferase Human genes 0.000 description 2
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 2
- 108010007784 Methionine adenosyltransferase Proteins 0.000 description 2
- 101100354186 Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) ptcA gene Proteins 0.000 description 2
- HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 108700040177 Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferases Proteins 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 101710113020 Ornithine transcarbamylase, mitochondrial Proteins 0.000 description 2
- 102000029785 Orotate phosphoribosyltransferase Human genes 0.000 description 2
- 102100037214 Orotidine 5'-phosphate decarboxylase Human genes 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 108010021592 Pantothenate kinase Proteins 0.000 description 2
- 102100024122 Pantothenate kinase 1 Human genes 0.000 description 2
- 108010086950 Phosphoribosylanthranilate isomerase Proteins 0.000 description 2
- 101710176328 Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] Proteins 0.000 description 2
- 102100037681 Protein FEV Human genes 0.000 description 2
- 101710198166 Protein FEV Proteins 0.000 description 2
- 101710150114 Protein rep Proteins 0.000 description 2
- 101100406344 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aceF gene Proteins 0.000 description 2
- 102100038517 Pyridoxal kinase Human genes 0.000 description 2
- 102000004477 Pyridoxine kinase Human genes 0.000 description 2
- 102000004518 Pyrroline-5-carboxylate reductase Human genes 0.000 description 2
- 108020005115 Pyruvate Kinase Proteins 0.000 description 2
- 102000013009 Pyruvate Kinase Human genes 0.000 description 2
- 101710152114 Replication protein Proteins 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 102000000439 Ribose-phosphate pyrophosphokinase Human genes 0.000 description 2
- ZJUKTBDSGOFHSH-WFMPWKQPSA-N S-Adenosylhomocysteine Chemical compound O[C@@H]1[C@H](O)[C@@H](CSCC[C@H](N)C(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZJUKTBDSGOFHSH-WFMPWKQPSA-N 0.000 description 2
- 102100026115 S-adenosylmethionine synthase isoform type-1 Human genes 0.000 description 2
- 101710099182 S-layer protein Proteins 0.000 description 2
- 108091022908 Serine O-acetyltransferase Proteins 0.000 description 2
- 102100035717 Serine racemase Human genes 0.000 description 2
- 108010006152 Serine racemase Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 108010056371 Succinyl-diaminopimelate desuccinylase Proteins 0.000 description 2
- 101710183296 Surface layer protein Proteins 0.000 description 2
- 108030000880 Thioethanolamine S-acetyltransferases Proteins 0.000 description 2
- 108090000190 Thrombin Proteins 0.000 description 2
- 102100037357 Thymidylate kinase Human genes 0.000 description 2
- 102000004357 Transferases Human genes 0.000 description 2
- 108090000992 Transferases Proteins 0.000 description 2
- 102100021869 Tyrosine aminotransferase Human genes 0.000 description 2
- 108010042606 Tyrosine transaminase Proteins 0.000 description 2
- 108020000553 UMP kinase Proteins 0.000 description 2
- 101710100179 UMP-CMP kinase Proteins 0.000 description 2
- 101710119674 UMP-CMP kinase 2, mitochondrial Proteins 0.000 description 2
- 108010064762 Uroporphyrinogen decarboxylase Proteins 0.000 description 2
- 102000015437 Uroporphyrinogen decarboxylase Human genes 0.000 description 2
- 101150036393 aceB gene Proteins 0.000 description 2
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 239000000556 agonist Substances 0.000 description 2
- PPQRONHOSHZGFQ-LMVFSUKVSA-N aldehydo-D-ribose 5-phosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PPQRONHOSHZGFQ-LMVFSUKVSA-N 0.000 description 2
- 230000003281 allosteric effect Effects 0.000 description 2
- 229960004050 aminobenzoic acid Drugs 0.000 description 2
- 229940126575 aminoglycoside Drugs 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 101150070427 argC gene Proteins 0.000 description 2
- 101150089042 argC2 gene Proteins 0.000 description 2
- 101150056313 argF gene Proteins 0.000 description 2
- 101150054318 argH gene Proteins 0.000 description 2
- 101150089004 argR gene Proteins 0.000 description 2
- 101150037081 aroA gene Proteins 0.000 description 2
- 101150090235 aroB gene Proteins 0.000 description 2
- 101150102858 aroD gene Proteins 0.000 description 2
- 101150108612 aroQ gene Proteins 0.000 description 2
- 108010002447 aspartate-alpha-decarboxylase Proteins 0.000 description 2
- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 2
- 229960003237 betaine Drugs 0.000 description 2
- 230000008238 biochemical pathway Effects 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000002742 combinatorial mutagenesis Methods 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 241000186254 coryneform bacterium Species 0.000 description 2
- 108700040031 dCTP deaminases Proteins 0.000 description 2
- 108010000742 dTMP kinase Proteins 0.000 description 2
- 101150011371 dapA gene Proteins 0.000 description 2
- 101150064923 dapD gene Proteins 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 108010082690 diaminopimelic acid decarboxylase Proteins 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000001177 diphosphate Substances 0.000 description 2
- 235000011180 diphosphates Nutrition 0.000 description 2
- 231100000676 disease causative agent Toxicity 0.000 description 2
- 101150027005 divIB gene Proteins 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 210000002889 endothelial cell Anatomy 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- 108091023030 exopolyphosphatase Proteins 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 229940013640 flavin mononucleotide Drugs 0.000 description 2
- FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 2
- 239000011768 flavin mononucleotide Substances 0.000 description 2
- FVTCRASFADXXNN-UHFFFAOYSA-N flavin mononucleotide Natural products OP(=O)(O)OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-UHFFFAOYSA-N 0.000 description 2
- 239000011724 folic acid Substances 0.000 description 2
- 101150002100 ftsK gene Proteins 0.000 description 2
- 101150026421 ftsQ gene Proteins 0.000 description 2
- 101150111615 ftsZ gene Proteins 0.000 description 2
- 230000005714 functional activity Effects 0.000 description 2
- 238000010230 functional analysis Methods 0.000 description 2
- 101150019455 gdh gene Proteins 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- 229960002449 glycine Drugs 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
- 102000006638 guanylate kinase Human genes 0.000 description 2
- 150000003278 haem Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 108010071598 homoserine kinase Proteins 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 101150118781 icd gene Proteins 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 description 2
- 235000019136 lipoic acid Nutrition 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 101150035025 lysC gene Proteins 0.000 description 2
- 101150094164 lysY gene Proteins 0.000 description 2
- 229960003646 lysine Drugs 0.000 description 2
- 108010039327 maltooligosyl trehalose synthase Proteins 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 2
- 235000013923 monosodium glutamate Nutrition 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 230000037360 nucleotide metabolism Effects 0.000 description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
- 101150021317 odhA gene Proteins 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 150000002894 organic compounds Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 101150076071 panD gene Proteins 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 102000029799 phosphatidate cytidylyltransferase Human genes 0.000 description 2
- 108091022886 phosphatidate cytidylyltransferase Proteins 0.000 description 2
- 108010088694 phosphoserine aminotransferase Proteins 0.000 description 2
- 238000013081 phylogenetic analysis Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- FPWMCUPFBRFMLH-UHFFFAOYSA-N prephenic acid Chemical compound OC1C=CC(CC(=O)C(O)=O)(C(O)=O)C=C1 FPWMCUPFBRFMLH-UHFFFAOYSA-N 0.000 description 2
- 239000002213 purine nucleotide Substances 0.000 description 2
- 108010019718 putrescine oxidase Proteins 0.000 description 2
- NHZMQXZHNVQTQA-UHFFFAOYSA-N pyridoxamine Chemical compound CC1=NC=C(CO)C(CN)=C1O NHZMQXZHNVQTQA-UHFFFAOYSA-N 0.000 description 2
- 235000008160 pyridoxine Nutrition 0.000 description 2
- 239000011677 pyridoxine Substances 0.000 description 2
- 108020001898 pyrroline-5-carboxylate reductase Proteins 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 101150079601 recA gene Proteins 0.000 description 2
- 238000010188 recombinant method Methods 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 235000019231 riboflavin-5'-phosphate Nutrition 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000007086 side reaction Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- HZSAJDVWZRBGIF-UHFFFAOYSA-O thiamine monophosphate Chemical compound CC1=C(CCOP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N HZSAJDVWZRBGIF-UHFFFAOYSA-O 0.000 description 2
- 229960002663 thioctic acid Drugs 0.000 description 2
- ODBLHEXUDAPZAU-UHFFFAOYSA-N threo-D-isocitric acid Natural products OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 108010045348 trehalose synthase Proteins 0.000 description 2
- 108020003272 trehalose-phosphatase Proteins 0.000 description 2
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 2
- 101150019416 trpA gene Proteins 0.000 description 2
- 101150100816 trpD gene Proteins 0.000 description 2
- 101150079930 trpGD gene Proteins 0.000 description 2
- 229960004799 tryptophan Drugs 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- AUTOLBMXDDTRRT-JGVFFNPUSA-N (4R,5S)-dethiobiotin Chemical compound C[C@@H]1NC(=O)N[C@@H]1CCCCCC(O)=O AUTOLBMXDDTRRT-JGVFFNPUSA-N 0.000 description 1
- OJJHFKVRJCQKLN-YFKPBYRVSA-N (4s)-4-acetamido-5-oxo-5-phosphonooxypentanoic acid Chemical compound OC(=O)CC[C@H](NC(=O)C)C(=O)OP(O)(O)=O OJJHFKVRJCQKLN-YFKPBYRVSA-N 0.000 description 1
- MSTNYGQPCMXVAQ-RYUDHWBXSA-N (6S)-5,6,7,8-tetrahydrofolic acid Chemical compound C([C@H]1CNC=2N=C(NC(=O)C=2N1)N)NC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 MSTNYGQPCMXVAQ-RYUDHWBXSA-N 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 1
- UWOCFOFVIBZJGH-YFKPBYRVSA-N (S)-2,3-dihydrodipicolinic acid Chemical compound OC(=O)[C@@H]1CC=CC(C(O)=O)=N1 UWOCFOFVIBZJGH-YFKPBYRVSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- BPQAUXWAEPYEJM-PQSJUMPYSA-N 1-hydroxy-8-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyanthracene-9,10-dione Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC1=CC=CC2=C1C(=O)C1=C(O)C=CC=C1C2=O BPQAUXWAEPYEJM-PQSJUMPYSA-N 0.000 description 1
- WJNGQIYEQLPJMN-IOSLPCCCSA-N 1-methylinosine Chemical compound C1=NC=2C(=O)N(C)C=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O WJNGQIYEQLPJMN-IOSLPCCCSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- UWOCFOFVIBZJGH-UHFFFAOYSA-N 2,3-dihydrodipicolinic acid Chemical compound OC(=O)C1CC=CC(C(O)=O)=N1 UWOCFOFVIBZJGH-UHFFFAOYSA-N 0.000 description 1
- HLYBTPMYFWWNJN-UHFFFAOYSA-N 2-(2,4-dioxo-1h-pyrimidin-5-yl)-2-hydroxyacetic acid Chemical compound OC(=O)C(O)C1=CNC(=O)NC1=O HLYBTPMYFWWNJN-UHFFFAOYSA-N 0.000 description 1
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- DHCUIDTZCMREHG-UHFFFAOYSA-N 2-(carboxymethyl)-5-oxo-2,5-dihydro-2-furoic acid Chemical compound OC(=O)CC1(C(O)=O)OC(=O)C=C1 DHCUIDTZCMREHG-UHFFFAOYSA-N 0.000 description 1
- SGAKLDIYNFXTCK-UHFFFAOYSA-N 2-[(2,4-dioxo-1h-pyrimidin-5-yl)methylamino]acetic acid Chemical compound OC(=O)CNCC1=CNC(=O)NC1=O SGAKLDIYNFXTCK-UHFFFAOYSA-N 0.000 description 1
- VKRFXNXJOJJPAO-UHFFFAOYSA-N 2-amino-4-(2,4-dioxo-1h-pyrimidin-3-yl)butanoic acid Chemical compound OC(=O)C(N)CCN1C(=O)C=CNC1=O VKRFXNXJOJJPAO-UHFFFAOYSA-N 0.000 description 1
- UDOGNMDURIJYQC-UHFFFAOYSA-N 2-amino-6-methyl-1h-pteridin-4-one Chemical compound N1C(N)=NC(=O)C2=NC(C)=CN=C21 UDOGNMDURIJYQC-UHFFFAOYSA-N 0.000 description 1
- XMSMHKMPBNTBOD-UHFFFAOYSA-N 2-dimethylamino-6-hydroxypurine Chemical compound N1C(N(C)C)=NC(=O)C2=C1N=CN2 XMSMHKMPBNTBOD-UHFFFAOYSA-N 0.000 description 1
- SMADWRYCYBUIKH-UHFFFAOYSA-N 2-methyl-7h-purin-6-amine Chemical compound CC1=NC(N)=C2NC=NC2=N1 SMADWRYCYBUIKH-UHFFFAOYSA-N 0.000 description 1
- 101710157142 2-methylene-furan-3-one reductase Proteins 0.000 description 1
- AQSRRZGQRFFFGS-UHFFFAOYSA-N 2-methylpyridin-3-ol Chemical compound CC1=NC=CC=C1O AQSRRZGQRFFFGS-UHFFFAOYSA-N 0.000 description 1
- 108010080376 3-Deoxy-7-Phosphoheptulonate Synthase Proteins 0.000 description 1
- KOLPWZCZXAMXKS-UHFFFAOYSA-N 3-methylcytosine Chemical compound CN1C(N)=CC=NC1=O KOLPWZCZXAMXKS-UHFFFAOYSA-N 0.000 description 1
- 108010043320 3-oxoadipate CoA-transferase Proteins 0.000 description 1
- 101710196797 3-oxoadipate CoA-transferase subunit B Proteins 0.000 description 1
- 108010000816 3-oxoadipate enol-lactonase Proteins 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- GJAKJCICANKRFD-UHFFFAOYSA-N 4-acetyl-4-amino-1,3-dihydropyrimidin-2-one Chemical compound CC(=O)C1(N)NC(=O)NC=C1 GJAKJCICANKRFD-UHFFFAOYSA-N 0.000 description 1
- 101710094518 4-aminobutyrate aminotransferase Proteins 0.000 description 1
- 108030000921 4-aminobutyrate-2-oxoglutarate transaminases Proteins 0.000 description 1
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 1
- 101710188260 5,10-methylenetetrahydrofolate reductase Proteins 0.000 description 1
- LJUQGASMPRMWIW-UHFFFAOYSA-N 5,6-dimethylbenzimidazole Chemical compound C1=C(C)C(C)=CC2=C1NC=N2 LJUQGASMPRMWIW-UHFFFAOYSA-N 0.000 description 1
- MQJSSLBGAQJNER-UHFFFAOYSA-N 5-(methylaminomethyl)-1h-pyrimidine-2,4-dione Chemical compound CNCC1=CNC(=O)NC1=O MQJSSLBGAQJNER-UHFFFAOYSA-N 0.000 description 1
- 108010075604 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase Proteins 0.000 description 1
- 102000011848 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase Human genes 0.000 description 1
- WPYRHVXCOQLYLY-UHFFFAOYSA-N 5-[(methoxyamino)methyl]-2-sulfanylidene-1h-pyrimidin-4-one Chemical compound CONCC1=CNC(=S)NC1=O WPYRHVXCOQLYLY-UHFFFAOYSA-N 0.000 description 1
- LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 description 1
- VKLFQTYNHLDMDP-PNHWDRBUSA-N 5-carboxymethylaminomethyl-2-thiouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=S)NC(=O)C(CNCC(O)=O)=C1 VKLFQTYNHLDMDP-PNHWDRBUSA-N 0.000 description 1
- ZFTBZKVVGZNMJR-UHFFFAOYSA-N 5-chlorouracil Chemical compound ClC1=CNC(=O)NC1=O ZFTBZKVVGZNMJR-UHFFFAOYSA-N 0.000 description 1
- LDCYZAJDBXYCGN-VIFPVBQESA-N 5-hydroxy-L-tryptophan Chemical compound C1=C(O)C=C2C(C[C@H](N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-VIFPVBQESA-N 0.000 description 1
- KSNXJLQDQOIRIP-UHFFFAOYSA-N 5-iodouracil Chemical compound IC1=CNC(=O)NC1=O KSNXJLQDQOIRIP-UHFFFAOYSA-N 0.000 description 1
- KELXHQACBIUYSE-UHFFFAOYSA-N 5-methoxy-1h-pyrimidine-2,4-dione Chemical compound COC1=CNC(=O)NC1=O KELXHQACBIUYSE-UHFFFAOYSA-N 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- 108020005075 5S Ribosomal RNA Proteins 0.000 description 1
- DCPSTSVLRXOYGS-UHFFFAOYSA-N 6-amino-1h-pyrimidine-2-thione Chemical compound NC1=CC=NC(S)=N1 DCPSTSVLRXOYGS-UHFFFAOYSA-N 0.000 description 1
- MSSXOMSJDRHRMC-UHFFFAOYSA-N 9H-purine-2,6-diamine Chemical compound NC1=NC(N)=C2NC=NC2=N1 MSSXOMSJDRHRMC-UHFFFAOYSA-N 0.000 description 1
- 101150103672 AMT gene Proteins 0.000 description 1
- 102000021527 ATP binding proteins Human genes 0.000 description 1
- 108091011108 ATP binding proteins Proteins 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 101710103719 Acetolactate synthase large subunit Proteins 0.000 description 1
- 101710182467 Acetolactate synthase large subunit IlvB1 Proteins 0.000 description 1
- 101710171176 Acetolactate synthase large subunit IlvG Proteins 0.000 description 1
- 101710176702 Acetolactate synthase small subunit Proteins 0.000 description 1
- 101710147947 Acetolactate synthase small subunit 1, chloroplastic Proteins 0.000 description 1
- 108700021045 Acetylglutamate kinase Proteins 0.000 description 1
- 108010009924 Aconitate hydratase Proteins 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 1
- 101710180630 Adenosylcobalamin/alpha-ribazole phosphatase Proteins 0.000 description 1
- 108030001147 Adenosylmethionine-8-amino-7-oxononanoate transaminases Proteins 0.000 description 1
- 102000005291 Adenylosuccinate synthase Human genes 0.000 description 1
- 101000889837 Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) Protein CysO Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 101710121865 Alanine racemase, biosynthetic Proteins 0.000 description 1
- 102100027211 Albumin Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 108090000072 Aldehyde-Lyases Proteins 0.000 description 1
- 102000003677 Aldehyde-Lyases Human genes 0.000 description 1
- 101100163490 Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) aroA1 gene Proteins 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 101710156975 Anthranilate synthase component 1 Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 241001167018 Aroa Species 0.000 description 1
- 102000030907 Aspartate Carbamoyltransferase Human genes 0.000 description 1
- 102100032948 Aspartoacylase Human genes 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 101000952999 Bacillus subtilis (strain 168) Putative esterase ComA2 Proteins 0.000 description 1
- 101100216993 Bacillus subtilis (strain 168) aroD gene Proteins 0.000 description 1
- 101100443932 Bacillus subtilis (strain 168) dppE gene Proteins 0.000 description 1
- 101100082080 Bacillus subtilis (strain 168) pabA gene Proteins 0.000 description 1
- 101100242035 Bacillus subtilis (strain 168) pdhA gene Proteins 0.000 description 1
- 101710201279 Biotin carboxyl carrier protein Proteins 0.000 description 1
- 108010018763 Biotin carboxylase Proteins 0.000 description 1
- 102100039866 CTP synthase 1 Human genes 0.000 description 1
- 101100441252 Caenorhabditis elegans csp-2 gene Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 240000001432 Calendula officinalis Species 0.000 description 1
- 235000005881 Calendula officinalis Nutrition 0.000 description 1
- 101100339117 Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) hisF1 gene Proteins 0.000 description 1
- 101710102665 Carbamoyl-phosphate synthase small chain Proteins 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 102100034229 Citramalyl-CoA lyase, mitochondrial Human genes 0.000 description 1
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 244000289527 Cordyline terminalis Species 0.000 description 1
- 235000009091 Cordyline terminalis Nutrition 0.000 description 1
- 108700031806 Corynebacterium diphtheriae DtxR Proteins 0.000 description 1
- 101000956566 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) L-lysine transport protein Proteins 0.000 description 1
- 101100332744 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) ectP gene Proteins 0.000 description 1
- 101100481367 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) thtR gene Proteins 0.000 description 1
- 101900213311 Corynebacterium glutamicum Threonine synthase Proteins 0.000 description 1
- 101710095468 Cyclase Proteins 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 102100039868 Cytoplasmic aconitate hydratase Human genes 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- NGHMDNPXVRFFGS-IUYQGCFVSA-N D-erythrose 4-phosphate Chemical compound O=C[C@H](O)[C@H](O)COP(O)(O)=O NGHMDNPXVRFFGS-IUYQGCFVSA-N 0.000 description 1
- 229930195713 D-glutamate Natural products 0.000 description 1
- WHUUTDBJXJRKMK-GSVOUGTGSA-N D-glutamic acid Chemical compound OC(=O)[C@H](N)CCC(O)=O WHUUTDBJXJRKMK-GSVOUGTGSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 229930182818 D-methionine Natural products 0.000 description 1
- FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 description 1
- 239000011703 D-panthenol Substances 0.000 description 1
- 235000004866 D-panthenol Nutrition 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 108020005199 Dehydrogenases Proteins 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 206010012442 Dermatitis contact Diseases 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 101710092096 Diaminopimelate decarboxylase Proteins 0.000 description 1
- 101100098219 Dictyostelium discoideum argS1 gene Proteins 0.000 description 1
- 101100012958 Dictyostelium discoideum fkbp3 gene Proteins 0.000 description 1
- 101100229207 Dictyostelium discoideum glnA3 gene Proteins 0.000 description 1
- 101100465553 Dictyostelium discoideum psmB6 gene Proteins 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 108030000716 Dihydrolipoyllysine-residue succinyltransferases Proteins 0.000 description 1
- 108010052167 Dihydroorotate Dehydrogenase Proteins 0.000 description 1
- 102100032823 Dihydroorotate dehydrogenase (quinone), mitochondrial Human genes 0.000 description 1
- 101710099431 Dimethyladenosine transferase Proteins 0.000 description 1
- 101710196859 Dipeptide-binding protein Proteins 0.000 description 1
- 101710155351 Diphtheria toxin repressor Proteins 0.000 description 1
- 108700034366 EC 3.5.4.25 Proteins 0.000 description 1
- 108700034205 EC 4.1.2.15 Proteins 0.000 description 1
- 108700033126 EC 4.1.3.12 Proteins 0.000 description 1
- 108700035159 EC 4.2.1.15 Proteins 0.000 description 1
- 108700035163 EC 4.2.1.16 Proteins 0.000 description 1
- 108700034030 EC 4.2.99.2 Proteins 0.000 description 1
- 108700033473 EC 6.3.4.1 Proteins 0.000 description 1
- 108700033475 EC 6.3.4.2 Proteins 0.000 description 1
- 108700035801 EC 6.3.5.1 Proteins 0.000 description 1
- 108700035791 EC 6.3.5.4 Proteins 0.000 description 1
- 239000004097 EU approved flavor enhancer Substances 0.000 description 1
- 108091006149 Electron carriers Proteins 0.000 description 1
- 101100491986 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) aromA gene Proteins 0.000 description 1
- 108010013369 Enteropeptidase Proteins 0.000 description 1
- 102100029727 Enteropeptidase Human genes 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 101100218845 Escherichia coli (strain K12) bioH gene Proteins 0.000 description 1
- 101710116650 FAD-dependent monooxygenase Proteins 0.000 description 1
- 108010074860 Factor Xa Proteins 0.000 description 1
- 206010051998 Febrile infection Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 101710187052 Flavohemoprotein Proteins 0.000 description 1
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 1
- 108010093223 Folylpolyglutamate synthetase Proteins 0.000 description 1
- QGWNDRXFNXRZMB-UUOKFMHZSA-K GDP(3-) Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O QGWNDRXFNXRZMB-UUOKFMHZSA-K 0.000 description 1
- 108010013942 GMP Reductase Proteins 0.000 description 1
- 102000017179 GMP reductase Human genes 0.000 description 1
- 108090000926 GMP synthase (glutamine-hydrolyzing) Proteins 0.000 description 1
- 101710071060 GMPS Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 206010071602 Genetic polymorphism Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000234 Glutamate synthase (NADH) Proteins 0.000 description 1
- 101710087963 Glutamate synthase [NADH] Proteins 0.000 description 1
- 101710128234 Glutamate synthase [NADPH] small chain Proteins 0.000 description 1
- 102000017722 Glutamine amidotransferases Human genes 0.000 description 1
- 108050005901 Glutamine amidotransferases Proteins 0.000 description 1
- 102100023518 Glutamine-dependent NAD(+) synthetase Human genes 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- 241000288105 Grus Species 0.000 description 1
- 102100032821 HIG1 domain family member 1C Human genes 0.000 description 1
- 101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 1
- 101000797251 Homo sapiens Aspartoacylase Proteins 0.000 description 1
- 101100284913 Homo sapiens HIGD1C gene Proteins 0.000 description 1
- 101001138544 Homo sapiens UMP-CMP kinase Proteins 0.000 description 1
- 241000701109 Human adenovirus 2 Species 0.000 description 1
- 108010027436 Hydroxymethylpyrimidine kinase Proteins 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 235000000177 Indigofera tinctoria Nutrition 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- 108010075869 Isocitrate Dehydrogenase Proteins 0.000 description 1
- 102000012011 Isocitrate Dehydrogenase Human genes 0.000 description 1
- 101100123255 Komagataeibacter xylinus aceC gene Proteins 0.000 description 1
- 235000019766 L-Lysine Nutrition 0.000 description 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- 150000008575 L-amino acids Chemical class 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
- GHSJKUNUIHUPDF-BYPYZUCNSA-N L-thialysine Chemical compound NCCSC[C@H](N)C(O)=O GHSJKUNUIHUPDF-BYPYZUCNSA-N 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 108050005713 Lipoate-protein ligase A Proteins 0.000 description 1
- 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 1
- 108020004687 Malate Synthase Proteins 0.000 description 1
- 101710124867 Malonyl CoA-acyl carrier protein transacylase Proteins 0.000 description 1
- 102100027329 Malonyl-CoA-acyl carrier protein transacylase, mitochondrial Human genes 0.000 description 1
- 101710108743 Malonyl-[acyl-carrier protein] O-methyltransferase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 101710141619 Meso-diaminopimelate D-dehydrogenase Proteins 0.000 description 1
- 101100242684 Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) panD1 gene Proteins 0.000 description 1
- 101100129629 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) mrtA gene Proteins 0.000 description 1
- 101100292591 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) mtrA1 gene Proteins 0.000 description 1
- 101100261636 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) trpB2 gene Proteins 0.000 description 1
- 108010006331 Methylmalonate-Semialdehyde Dehydrogenase (Acylating) Proteins 0.000 description 1
- 102100029676 Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial Human genes 0.000 description 1
- 241000589327 Methylobacillus flagellatus Species 0.000 description 1
- 102000014842 Multidrug resistance proteins Human genes 0.000 description 1
- 108050005144 Multidrug resistance proteins Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 108010021466 Mutant Proteins Proteins 0.000 description 1
- 102000008300 Mutant Proteins Human genes 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- JRLGPAXAGHMNOL-LURJTMIESA-N N(2)-acetyl-L-ornithine Chemical compound CC(=O)N[C@H](C([O-])=O)CCC[NH3+] JRLGPAXAGHMNOL-LURJTMIESA-N 0.000 description 1
- SGSSKEDGVONRGC-UHFFFAOYSA-N N(2)-methylguanine Chemical compound O=C1NC(NC)=NC2=C1N=CN2 SGSSKEDGVONRGC-UHFFFAOYSA-N 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 108010086428 NADH Dehydrogenase Proteins 0.000 description 1
- 102000006746 NADH Dehydrogenase Human genes 0.000 description 1
- 108050008664 NH(3)-dependent NAD(+) synthetases Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000008763 Neurofilament Proteins Human genes 0.000 description 1
- 108010088373 Neurofilament Proteins Proteins 0.000 description 1
- 102100030830 Nicotinate-nucleotide pyrophosphorylase [carboxylating] Human genes 0.000 description 1
- 239000006057 Non-nutritive feed additive Substances 0.000 description 1
- 102000003832 Nucleotidyltransferases Human genes 0.000 description 1
- 108090000119 Nucleotidyltransferases Proteins 0.000 description 1
- 101710128228 O-methyltransferase Proteins 0.000 description 1
- 101710154896 Octanoyltransferase Proteins 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 108700006307 Ornithine carbamoyltransferases Proteins 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 101150093941 PORA gene Proteins 0.000 description 1
- ZNXZGRMVNNHPCA-UHFFFAOYSA-N Pantetheine Natural products OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS ZNXZGRMVNNHPCA-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108010069013 Phenylalanine Hydroxylase Proteins 0.000 description 1
- 102100038223 Phenylalanine-4-hydroxylase Human genes 0.000 description 1
- 101710116000 Phosphoadenosine phosphosulfate reductase Proteins 0.000 description 1
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 description 1
- 108010045040 Phosphoribosylaminoimidazolecarboxamide formyltransferase Proteins 0.000 description 1
- 108090000434 Phosphoribosylaminoimidazolesuccinocarboxamide synthases Proteins 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 102100021768 Phosphoserine aminotransferase Human genes 0.000 description 1
- 108010013381 Porins Proteins 0.000 description 1
- 102000017033 Porins Human genes 0.000 description 1
- 101710196435 Probable acetolactate synthase large subunit Proteins 0.000 description 1
- 101710181764 Probable acetolactate synthase small subunit Proteins 0.000 description 1
- 101710100290 Probable octanoyltransferase Proteins 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 101100134871 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aceE gene Proteins 0.000 description 1
- 101100217185 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) aruC gene Proteins 0.000 description 1
- 101100297447 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) phnB gene Proteins 0.000 description 1
- 108030005599 Pseudouridylate synthases Proteins 0.000 description 1
- 101710104000 Putative acetolactate synthase small subunit Proteins 0.000 description 1
- 101710089268 Putative lipoyltransferase 2, mitochondrial Proteins 0.000 description 1
- 102100026021 Putative lipoyltransferase 2, mitochondrial Human genes 0.000 description 1
- 101100169519 Pyrococcus abyssi (strain GE5 / Orsay) dapAL gene Proteins 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 108010053763 Pyruvate Carboxylase Proteins 0.000 description 1
- 108010090051 Pyruvate Dehydrogenase Complex Proteins 0.000 description 1
- 102000012751 Pyruvate Dehydrogenase Complex Human genes 0.000 description 1
- 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 1
- 101710189291 Quinone oxidoreductase Proteins 0.000 description 1
- 102100034576 Quinone oxidoreductase Human genes 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 108090001066 Racemases and epimerases Proteins 0.000 description 1
- 102000004879 Racemases and epimerases Human genes 0.000 description 1
- 101150070524 Rel gene Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 102100022604 Ribose-phosphate pyrophosphokinase 3 Human genes 0.000 description 1
- GBFLZEXEOZUWRN-VKHMYHEASA-M S-carboxylatomethyl-L-cysteine(1-) Chemical compound [O-]C(=O)[C@@H]([NH3+])CSCC([O-])=O GBFLZEXEOZUWRN-VKHMYHEASA-M 0.000 description 1
- 101100434411 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ADH1 gene Proteins 0.000 description 1
- 101000703543 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 6,7-dimethyl-8-ribityllumazine synthase Proteins 0.000 description 1
- 241000252141 Semionotiformes Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 108010051611 Signal Recognition Particle Proteins 0.000 description 1
- 102000013598 Signal recognition particle Human genes 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 101100125907 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) ilvC1 gene Proteins 0.000 description 1
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 101100022072 Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) lysJ gene Proteins 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 241000192581 Synechocystis sp. Species 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 241000223892 Tetrahymena Species 0.000 description 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
- 108030007080 Thiamine-phosphate kinases Proteins 0.000 description 1
- FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical group C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 108010022173 Thiosulfate sulfurtransferase Proteins 0.000 description 1
- 102100034707 Thiosulfate sulfurtransferase Human genes 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 102100033451 Thyroid hormone receptor beta Human genes 0.000 description 1
- 108090000210 Transferred entry: 1.5.1.20 Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 102000014701 Transketolase Human genes 0.000 description 1
- 108010043652 Transketolase Proteins 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 101100187081 Trichormus variabilis (strain ATCC 29413 / PCC 7937) nifS1 gene Proteins 0.000 description 1
- 101710151098 Tryptophan synthase alpha chain Proteins 0.000 description 1
- 102100020797 UMP-CMP kinase Human genes 0.000 description 1
- 102100032947 UMP-CMP kinase 2, mitochondrial Human genes 0.000 description 1
- 108030007083 UMP/CMP kinases Proteins 0.000 description 1
- ZVNYJIZDIRKMBF-UHFFFAOYSA-N Vesnarinone Chemical compound C1=C(OC)C(OC)=CC=C1C(=O)N1CCN(C=2C=C3CCC(=O)NC3=CC=2)CC1 ZVNYJIZDIRKMBF-UHFFFAOYSA-N 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- PFRQBZFETXBLTP-UHFFFAOYSA-N Vitamin K2 Natural products C1=CC=C2C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C(=O)C2=C1 PFRQBZFETXBLTP-UHFFFAOYSA-N 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 108010027570 Xanthine phosphoribosyltransferase Proteins 0.000 description 1
- 101000649206 Xanthomonas campestris pv. campestris (strain 8004) Uridine 5'-monophosphate transferase Proteins 0.000 description 1
- INAPMGSXUVUWAF-GCVPSNMTSA-N [(2r,3s,5r,6r)-2,3,4,5,6-pentahydroxycyclohexyl] dihydrogen phosphate Chemical compound OC1[C@H](O)[C@@H](O)C(OP(O)(O)=O)[C@H](O)[C@@H]1O INAPMGSXUVUWAF-GCVPSNMTSA-N 0.000 description 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
- BUFLLCUFNHESEH-UHFFFAOYSA-N [5-(2-amino-6-oxo-3h-purin-9-yl)-4-hydroxy-2-[[hydroxy(phosphonooxy)phosphoryl]oxymethyl]oxolan-3-yl] phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(=O)OP(O)(O)=O)C(OP(O)(=O)OP(O)(O)=O)C1O BUFLLCUFNHESEH-UHFFFAOYSA-N 0.000 description 1
- 101150094017 aceA gene Proteins 0.000 description 1
- 229960004308 acetylcysteine Drugs 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 125000000641 acridinyl group Chemical group C1(=CC=CC2=NC3=CC=CC=C3C=C12)* 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 101150102866 adc1 gene Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 102000005130 adenylosuccinate synthetase Human genes 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000037354 amino acid metabolism Effects 0.000 description 1
- 229940067621 aminobutyrate Drugs 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001028 anti-proliverative effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- SCJNCDSAIRBRIA-DOFZRALJSA-N arachidonyl-2'-chloroethylamide Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(=O)NCCCl SCJNCDSAIRBRIA-DOFZRALJSA-N 0.000 description 1
- 101150008194 argB gene Proteins 0.000 description 1
- 101150050866 argD gene Proteins 0.000 description 1
- 101150118463 argG gene Proteins 0.000 description 1
- 101150029940 argJ gene Proteins 0.000 description 1
- 101150024756 argS gene Proteins 0.000 description 1
- 101150042732 aroC gene Proteins 0.000 description 1
- 101150083869 aroK gene Proteins 0.000 description 1
- 108010048088 aromatic NADH-dependent nitroreductase Proteins 0.000 description 1
- 150000004982 aromatic amines Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 108010056089 asparagine synthetase (glutamine-hydrolyzing) Proteins 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 101150070136 axeA gene Proteins 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Chemical group C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 229940000635 beta-alanine Drugs 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000007068 beta-elimination reaction Methods 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 101150029327 bioB gene Proteins 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000013378 biophysical characterization Methods 0.000 description 1
- 230000001486 biosynthesis of amino acids Effects 0.000 description 1
- 150000005693 branched-chain amino acids Chemical class 0.000 description 1
- 101150098189 brnQ gene Proteins 0.000 description 1
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 102000028406 carbon-sulfur lyase Human genes 0.000 description 1
- 108010076637 carbon-sulfur lyase Proteins 0.000 description 1
- 150000003857 carboxamides Chemical class 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000000423 cell based assay Methods 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000012412 chemical coupling Methods 0.000 description 1
- 238000012824 chemical production Methods 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 101150071282 cipB gene Proteins 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 125000003346 cobalamin group Chemical group 0.000 description 1
- ASARMUCNOOHMLO-WLORSUFZSA-L cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2s)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O ASARMUCNOOHMLO-WLORSUFZSA-L 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 101150049887 cspB gene Proteins 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 101150073654 dapB gene Proteins 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000005786 degenerative changes Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000002074 deregulated effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 108010050073 dethiobiotin synthetase Proteins 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 108010079167 dihydrolipoamide succinyltransferase Proteins 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 108040007096 enoyl-[acyl-carrier-protein] reductase activity proteins Proteins 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 101150111583 fda gene Proteins 0.000 description 1
- 230000008713 feedback mechanism Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 229960002949 fluorouracil Drugs 0.000 description 1
- 101150075316 folE gene Proteins 0.000 description 1
- 229940014144 folate Drugs 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 235000019264 food flavour enhancer Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000013611 frozen food Nutrition 0.000 description 1
- 101150049376 ftsY gene Proteins 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 230000008303 genetic mechanism Effects 0.000 description 1
- 101150019449 glnB gene Proteins 0.000 description 1
- 101150106096 gltA gene Proteins 0.000 description 1
- 101150041871 gltB gene Proteins 0.000 description 1
- 101150039906 gltD gene Proteins 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 108010050322 glutamate acetyltransferase Proteins 0.000 description 1
- 108010016102 glutamine transport proteins Proteins 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 101150036612 gnl gene Proteins 0.000 description 1
- QGWNDRXFNXRZMB-UHFFFAOYSA-N guanidine diphosphate Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O QGWNDRXFNXRZMB-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 108010064833 guanylyltransferase Proteins 0.000 description 1
- 230000007407 health benefit Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 101150050908 hisA gene Proteins 0.000 description 1
- 101150056694 hisC gene Proteins 0.000 description 1
- 101150054929 hisE gene Proteins 0.000 description 1
- 101150096813 hisF gene Proteins 0.000 description 1
- 101150032598 hisG gene Proteins 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 101150090497 ilvC gene Proteins 0.000 description 1
- 101150077793 ilvH gene Proteins 0.000 description 1
- 101150060643 ilvN gene Proteins 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 229960003444 immunosuppressant agent Drugs 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 101150009044 impA gene Proteins 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 229940097275 indigo Drugs 0.000 description 1
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 101150021879 iscS gene Proteins 0.000 description 1
- 108010029918 isocitrate dehydrogenase (NADP+) Proteins 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 101150039489 lysZ gene Proteins 0.000 description 1
- 229940049920 malate Drugs 0.000 description 1
- 108010076172 malate dehydrogenase (acceptor) Proteins 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 108010074674 maltooligosyl trehalose trehalohydrolase Proteins 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 230000037345 metabolism of vitamins Effects 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- IZAGSTRIDUNNOY-UHFFFAOYSA-N methyl 2-[(2,4-dioxo-1h-pyrimidin-5-yl)oxy]acetate Chemical compound COC(=O)COC1=CNC(=O)NC1=O IZAGSTRIDUNNOY-UHFFFAOYSA-N 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 101150069500 mtrA gene Proteins 0.000 description 1
- 230000036457 multidrug resistance Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- XJVXMWNLQRTRGH-UHFFFAOYSA-N n-(3-methylbut-3-enyl)-2-methylsulfanyl-7h-purin-6-amine Chemical compound CSC1=NC(NCCC(C)=C)=C2NC=NC2=N1 XJVXMWNLQRTRGH-UHFFFAOYSA-N 0.000 description 1
- 210000004898 n-terminal fragment Anatomy 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 210000005044 neurofilament Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 108090000277 nicotinate-nucleotide diphosphorylase (carboxylating) Proteins 0.000 description 1
- 101150082753 nifS gene Proteins 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 230000004145 nucleotide salvage Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 101150048280 ocd gene Proteins 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 238000012261 overproduction Methods 0.000 description 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
- LDCYZAJDBXYCGN-UHFFFAOYSA-N oxitriptan Natural products C1=C(O)C=C2C(CC(N)C(O)=O)=CNC2=C1 LDCYZAJDBXYCGN-UHFFFAOYSA-N 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- ZNXZGRMVNNHPCA-VIFPVBQESA-N pantetheine Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS ZNXZGRMVNNHPCA-VIFPVBQESA-N 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000004108 pentose phosphate pathway Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 238000010647 peptide synthesis reaction Methods 0.000 description 1
- 210000000578 peripheral nerve Anatomy 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 101150023849 pheA gene Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 102000030592 phosphoserine aminotransferase Human genes 0.000 description 1
- LYCRXMTYUZDUGA-UYRKPTJQSA-N pimeloyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCCCCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LYCRXMTYUZDUGA-UYRKPTJQSA-N 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 125000000830 polyketide group Chemical group 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 101150023641 ppc gene Proteins 0.000 description 1
- 235000013324 preserved food Nutrition 0.000 description 1
- 101150077403 priA gene Proteins 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 229940076376 protein agonist Drugs 0.000 description 1
- 229940076372 protein antagonist Drugs 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000006337 proteolytic cleavage Effects 0.000 description 1
- 108010016842 pterin-4a-carbinolamine dehydratase Proteins 0.000 description 1
- 101150096049 pyc gene Proteins 0.000 description 1
- OYSBZLVHMPNJMR-UHFFFAOYSA-N pyridine-3-carboxylic acid Chemical compound OC(=O)C1=CC=CN=C1.OC(=O)C1=CC=CN=C1 OYSBZLVHMPNJMR-UHFFFAOYSA-N 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 229960003581 pyridoxal Drugs 0.000 description 1
- 235000008164 pyridoxal Nutrition 0.000 description 1
- 239000011674 pyridoxal Substances 0.000 description 1
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 1
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 1
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 1
- 235000008151 pyridoxamine Nutrition 0.000 description 1
- 239000011699 pyridoxamine Substances 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006268 reductive amination reaction Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 108700021653 rel Genes Proteins 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 235000003441 saturated fatty acids Nutrition 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 101150114545 secE gene Proteins 0.000 description 1
- 101150004862 secG gene Proteins 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 101150112901 soxA gene Proteins 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000003153 stable transfection Methods 0.000 description 1
- 238000012409 standard PCR amplification Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 101150111745 sucA gene Proteins 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 239000005460 tetrahydrofolate Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 101150000850 thrC gene Proteins 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 101150106737 tpd gene Proteins 0.000 description 1
- 238000005891 transamination reaction Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 125000002264 triphosphate group Chemical group [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 101150035767 trp gene Proteins 0.000 description 1
- 101150081616 trpB gene Proteins 0.000 description 1
- 101150111232 trpB-1 gene Proteins 0.000 description 1
- 101150016309 trpC gene Proteins 0.000 description 1
- 101150034486 trpG gene Proteins 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 229940035936 ubiquinone Drugs 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 230000004143 urea cycle Effects 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 235000012141 vanillin Nutrition 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019143 vitamin K2 Nutrition 0.000 description 1
- 239000011728 vitamin K2 Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- WCNMEQDMUYVWMJ-JPZHCBQBSA-N wybutoxosine Chemical compound C1=NC=2C(=O)N3C(CC([C@H](NC(=O)OC)C(=O)OC)OO)=C(C)N=C3N(C)C=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O WCNMEQDMUYVWMJ-JPZHCBQBSA-N 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (117)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14103199P | 1999-06-25 | 1999-06-25 | |
US60/141,031 | 1999-06-25 | ||
DE19930476 | 1999-07-01 | ||
DE19930476.9 | 1999-07-01 | ||
US14210199P | 1999-07-02 | 1999-07-02 | |
US60/142,101 | 1999-07-02 | ||
DE19942088.2 | 1999-07-03 | ||
DE19931415 | 1999-07-08 | ||
DE19931632.5 | 1999-07-08 | ||
DE19931573.6 | 1999-07-08 | ||
DE19931453 | 1999-07-08 | ||
DE19931443.8 | 1999-07-08 | ||
DE19931418 | 1999-07-08 | ||
DE19931453.5 | 1999-07-08 | ||
DE19931592.2 | 1999-07-08 | ||
DE19931465.9 | 1999-07-08 | ||
DE19931634.1 | 1999-07-08 | ||
DE19931418.7 | 1999-07-08 | ||
DE19931573 | 1999-07-08 | ||
DE19931424 | 1999-07-08 | ||
DE19931420.9 | 1999-07-08 | ||
DE19931592 | 1999-07-08 | ||
DE19931636 | 1999-07-08 | ||
DE19931634 | 1999-07-08 | ||
DE19931428.4 | 1999-07-08 | ||
DE19931434.9 | 1999-07-08 | ||
DE19931478.0 | 1999-07-08 | ||
DE19931510 | 1999-07-08 | ||
DE19931541 | 1999-07-08 | ||
DE19931435 | 1999-07-08 | ||
DE19931434 | 1999-07-08 | ||
DE19931457.8 | 1999-07-08 | ||
DE19931419.5 | 1999-07-08 | ||
DE19931632 | 1999-07-08 | ||
DE19931465 | 1999-07-08 | ||
DE19931457 | 1999-07-08 | ||
DE19931424.1 | 1999-07-08 | ||
DE19931510.8 | 1999-07-08 | ||
DE19931443 | 1999-07-08 | ||
DE19931420 | 1999-07-08 | ||
DE19931435.7 | 1999-07-08 | ||
DE19931636.8 | 1999-07-08 | ||
DE19931415.2 | 1999-07-08 | ||
DE19931541.8 | 1999-07-08 | ||
DE19931419 | 1999-07-08 | ||
DE19931428 | 1999-07-08 | ||
DE19931478 | 1999-07-08 | ||
DE19932125.6 | 1999-07-09 | ||
DE19932228.7 | 1999-07-09 | ||
DE19932126.4 | 1999-07-09 | ||
DE19932186.8 | 1999-07-09 | ||
DE19932126 | 1999-07-09 | ||
DE19932130.2 | 1999-07-09 | ||
DE19932230 | 1999-07-09 | ||
DE19932206 | 1999-07-09 | ||
DE19932229.5 | 1999-07-09 | ||
DE19932228 | 1999-07-09 | ||
DE19932186 | 1999-07-09 | ||
DE19932227.9 | 1999-07-09 | ||
DE19932230.9 | 1999-07-09 | ||
DE19932229 | 1999-07-09 | ||
DE19932206.6 | 1999-07-09 | ||
DE19932227 | 1999-07-09 | ||
DE19932130 | 1999-07-09 | ||
DE19932125 | 1999-07-09 | ||
DE19933004 | 1999-07-14 | ||
DE19932922 | 1999-07-14 | ||
DE19933005 | 1999-07-14 | ||
DE19933006 | 1999-07-14 | ||
DE19933005.0 | 1999-07-14 | ||
DE19933006.9 | 1999-07-14 | ||
DE19932926 | 1999-07-14 | ||
DE19932922.2 | 1999-07-14 | ||
DE19932926.5 | 1999-07-14 | ||
DE19933004.2 | 1999-07-14 | ||
DE19932928.1 | 1999-07-14 | ||
DE19932928 | 1999-07-14 | ||
US14861399P | 1999-08-12 | 1999-08-12 | |
US60/148,613 | 1999-08-12 | ||
DE19940764.9 | 1999-08-27 | ||
DE19940832 | 1999-08-27 | ||
DE19940765.7 | 1999-08-27 | ||
DE19940764 | 1999-08-27 | ||
DE19940832.7 | 1999-08-27 | ||
DE19940765 | 1999-08-27 | ||
DE19940766 | 1999-08-27 | ||
DE19940766.5 | 1999-08-27 | ||
DE19941380 | 1999-08-31 | ||
DE19941394 | 1999-08-31 | ||
DE19941396.7 | 1999-08-31 | ||
DE19941394.0 | 1999-08-31 | ||
DE19941379 | 1999-08-31 | ||
DE19941378 | 1999-08-31 | ||
DE19941396 | 1999-08-31 | ||
DE19941378.9 | 1999-08-31 | ||
DE19941379.7 | 1999-08-31 | ||
DE19941380.0 | 1999-08-31 | ||
DE19942076 | 1999-09-03 | ||
DE19942129 | 1999-09-03 | ||
DE19942095.5 | 1999-09-03 | ||
DE19942077 | 1999-09-03 | ||
DE19942079.3 | 1999-09-03 | ||
DE19942086 | 1999-09-03 | ||
DE19942129.3 | 1999-09-03 | ||
DE19942095 | 1999-09-03 | ||
DE19942088 | 1999-09-03 | ||
DE19942077.7 | 1999-09-03 | ||
DE19942086.6 | 1999-09-03 | ||
DE19942124 | 1999-09-03 | ||
DE19942076.9 | 1999-09-03 | ||
DE19942087.4 | 1999-09-03 | ||
DE19942087 | 1999-09-03 | ||
DE19942079 | 1999-09-03 | ||
DE19942124.2 | 1999-09-03 | ||
US18797000P | 2000-03-09 | 2000-03-09 | |
US60/187,970 | 2000-03-09 | ||
CA002383865A CA2383865A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002383865A Division CA2383865A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2590662A1 true CA2590662A1 (fr) | 2001-01-04 |
Family
ID=38445927
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002590662A Abandoned CA2590662A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique |
CA002592965A Abandoned CA2592965A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002592965A Abandoned CA2592965A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique |
Country Status (1)
Country | Link |
---|---|
CA (2) | CA2590662A1 (fr) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112662605A (zh) * | 2020-12-30 | 2021-04-16 | 宁夏伊品生物科技股份有限公司 | Yh66_10715基因改造的高产l-异亮氨酸的菌株及其构建方法和应用 |
CN113528366A (zh) * | 2021-07-15 | 2021-10-22 | 洛阳华荣生物技术有限公司 | 一种产beta-丙氨酸酵母菌及其构建方法 |
CN114645051A (zh) * | 2016-09-15 | 2022-06-21 | 日本电气方案创新株式会社 | 分泌型免疫球蛋白A(sIgA)结合核酸分子、sIgA分析用传感器和sIgA分析方法 |
CN115028694A (zh) * | 2022-06-28 | 2022-09-09 | 内蒙古伊品生物科技有限公司 | 与l-谷氨酸产量相关的蛋白及其相关生物材料和应用 |
CN117778425A (zh) * | 2024-02-26 | 2024-03-29 | 中国农业科学院生物技术研究所 | 一种甲基转移酶在增强固氮微生物的固氮能力方面的用途和方法 |
-
2000
- 2000-06-23 CA CA002590662A patent/CA2590662A1/fr not_active Abandoned
- 2000-06-23 CA CA002592965A patent/CA2592965A1/fr not_active Abandoned
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114645051A (zh) * | 2016-09-15 | 2022-06-21 | 日本电气方案创新株式会社 | 分泌型免疫球蛋白A(sIgA)结合核酸分子、sIgA分析用传感器和sIgA分析方法 |
CN112662605A (zh) * | 2020-12-30 | 2021-04-16 | 宁夏伊品生物科技股份有限公司 | Yh66_10715基因改造的高产l-异亮氨酸的菌株及其构建方法和应用 |
CN113528366A (zh) * | 2021-07-15 | 2021-10-22 | 洛阳华荣生物技术有限公司 | 一种产beta-丙氨酸酵母菌及其构建方法 |
CN113528366B (zh) * | 2021-07-15 | 2023-12-08 | 洛阳华荣生物技术有限公司 | 一种产beta-丙氨酸酵母菌及其构建方法 |
CN115028694A (zh) * | 2022-06-28 | 2022-09-09 | 内蒙古伊品生物科技有限公司 | 与l-谷氨酸产量相关的蛋白及其相关生物材料和应用 |
CN117778425A (zh) * | 2024-02-26 | 2024-03-29 | 中国农业科学院生物技术研究所 | 一种甲基转移酶在增强固氮微生物的固氮能力方面的用途和方法 |
CN117778425B (zh) * | 2024-02-26 | 2024-06-04 | 中国农业科学院生物技术研究所 | 一种甲基转移酶在增强固氮微生物的固氮能力方面的用途和方法 |
Also Published As
Publication number | Publication date |
---|---|
CA2592965A1 (fr) | 2001-01-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7439050B2 (en) | Corynebacterium glutamicum genes encoding diaminopimelate epimerase | |
US7510854B2 (en) | Corynebacterium glutamicum genes encoding metabolic pathway proteins | |
EP1261718B1 (fr) | Genes de corynebacterium glutamicum codant des proteines de voie metabolique | |
US20070111230A1 (en) | Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport | |
US20070015252A1 (en) | Corynebacterium glutamicum genes encoding regulatory proteins | |
US20070231259A1 (en) | Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production | |
EP2292763A1 (fr) | Proteines codant pour les genes corynebacterium glutamicum, intervenant dans le metabolisme du carbone et dans la production d'energie | |
US7425435B2 (en) | Corynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins | |
CN101082049A (zh) | 编码代谢途径蛋白的谷氨酸棒杆菌基因 | |
US20070059810A1 (en) | Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins | |
EP2302058A1 (fr) | Genes de Corynebacterium glutamicum codant des protéines impliquées dans l'homéostase et adaptation | |
AU2001223903A1 (en) | Corynebacterium glutamicum genes encoding metabolic pathway proteins | |
US20060269975A1 (en) | Corynebacterium glutamicum genes encoding proteins involved in DNA replication, protein synthesis, and pathogenesis | |
EP1246922B1 (fr) | Genes de corynebacterium glutamicum codant pour des proteines du systeme de transport des sucres phosphotransferase: phosphoenolpyruvate | |
CA2590662A1 (fr) | Genes de corynebacterum glutamicum codant pour des proteines de voie metabolique | |
AU783709B2 (en) | Corynebacterium glutamicum genes encoding metabolic pathway proteins | |
AU2006200807A1 (en) | Coryneracterium glutamicum genes encoding metabolic pathway proteins | |
CA2584021A1 (fr) | Proteines codant pour les genes corynebacterium glutamicum, intervenant dans le metabolisme du carbone et dans la production d'energie | |
AU2007203039A1 (en) | Corynebacterium glutamicum genese encoding proteins involved in carbon metabolism and energy production | |
AU2007202378A1 (en) | Coryneracterium glutamicum genes encoding metabolic pathway proteins | |
EP1702980A1 (fr) | Gène issu de Corynebacterium glutamicum codant pour Hpr de système de phosphoenolpyruvate:sucre phosphotransferase | |
CA2590403A1 (fr) | Genes de corynebacterium glutamicum codant le phosphoenolpyruvate: proteines du systeme des sucres phosphotransferase | |
CA2585184A1 (fr) | Genes corynebacterium glutamicum codant des proteines de stress, resistance et tolerance | |
AU2007202394A1 (en) | Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins | |
AU2006252111A1 (en) | Corynebacterium glutamicum genes encoding metabolic pathway proteins |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Dead |