CA2586415A1 - Methode de detection de la modification conformationnelle de la calmoduline et methode de criblage de substances dont l'activite a des effets sur la modification conformationnellede la calmoduline - Google Patents
Methode de detection de la modification conformationnelle de la calmoduline et methode de criblage de substances dont l'activite a des effets sur la modification conformationnellede la calmoduline Download PDFInfo
- Publication number
- CA2586415A1 CA2586415A1 CA002586415A CA2586415A CA2586415A1 CA 2586415 A1 CA2586415 A1 CA 2586415A1 CA 002586415 A CA002586415 A CA 002586415A CA 2586415 A CA2586415 A CA 2586415A CA 2586415 A1 CA2586415 A1 CA 2586415A1
- Authority
- CA
- Canada
- Prior art keywords
- calmodulin
- protein
- sample film
- kinase
- conformational change
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 102000000584 Calmodulin Human genes 0.000 title claims abstract description 178
- 108010041952 Calmodulin Proteins 0.000 title claims abstract description 178
- 238000000034 method Methods 0.000 title claims abstract description 70
- 230000008859 change Effects 0.000 title claims abstract description 64
- 230000000694 effects Effects 0.000 title claims abstract description 29
- 239000000126 substance Substances 0.000 title claims description 46
- 238000012216 screening Methods 0.000 title claims description 15
- 238000012360 testing method Methods 0.000 claims abstract description 14
- 102000004169 proteins and genes Human genes 0.000 claims description 45
- 108090000623 proteins and genes Proteins 0.000 claims description 45
- 230000001419 dependent effect Effects 0.000 claims description 31
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 239000000758 substrate Substances 0.000 claims description 20
- 239000012634 fragment Substances 0.000 claims description 16
- 239000011575 calcium Substances 0.000 claims description 14
- 229910052791 calcium Inorganic materials 0.000 claims description 14
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 13
- 239000003814 drug Substances 0.000 claims description 6
- 102000004631 Calcineurin Human genes 0.000 claims description 5
- 108010042955 Calcineurin Proteins 0.000 claims description 5
- 102000004657 Calcium-Calmodulin-Dependent Protein Kinase Type 2 Human genes 0.000 claims description 5
- 108010003721 Calcium-Calmodulin-Dependent Protein Kinase Type 2 Proteins 0.000 claims description 5
- 239000000032 diagnostic agent Substances 0.000 claims description 5
- 229940039227 diagnostic agent Drugs 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 229940124597 therapeutic agent Drugs 0.000 claims description 5
- 102000004646 Calcium-Calmodulin-Dependent Protein Kinase Type 4 Human genes 0.000 claims description 4
- 108010003613 Calcium-Calmodulin-Dependent Protein Kinase Type 4 Proteins 0.000 claims description 4
- 101000584208 Homo sapiens Myosin light chain kinase 2, skeletal/cardiac muscle Proteins 0.000 claims description 4
- 101001022780 Homo sapiens Myosin light chain kinase, smooth muscle Proteins 0.000 claims description 4
- 101000909851 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) cAMP/cGMP dual specificity phosphodiesterase Rv0805 Proteins 0.000 claims description 4
- 102100030788 Myosin light chain kinase 2, skeletal/cardiac muscle Human genes 0.000 claims description 4
- 102100035044 Myosin light chain kinase, smooth muscle Human genes 0.000 claims description 4
- 102000006538 Nitric Oxide Synthase Type I Human genes 0.000 claims description 4
- 108010008858 Nitric Oxide Synthase Type I Proteins 0.000 claims description 4
- 108091007960 PI3Ks Proteins 0.000 claims description 4
- 102000038030 PI3Ks Human genes 0.000 claims description 4
- 108010064071 Phosphorylase Kinase Proteins 0.000 claims description 4
- 102000014750 Phosphorylase Kinase Human genes 0.000 claims description 4
- 102100026773 Unconventional myosin-Ia Human genes 0.000 claims description 4
- 101710135389 Unconventional myosin-Ia Proteins 0.000 claims description 4
- BAWFJGJZGIEFAR-DQQFMEOOSA-N [[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)O[P@@](O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-DQQFMEOOSA-N 0.000 claims description 4
- 102000030621 adenylate cyclase Human genes 0.000 claims description 4
- 108060000200 adenylate cyclase Proteins 0.000 claims description 4
- 210000000601 blood cell Anatomy 0.000 claims description 4
- 102000028861 calmodulin binding Human genes 0.000 claims description 4
- 108091000084 calmodulin binding Proteins 0.000 claims description 4
- 230000031146 intracellular signal transduction Effects 0.000 claims description 3
- 230000001225 therapeutic effect Effects 0.000 claims description 3
- 239000010408 film Substances 0.000 description 55
- 239000007853 buffer solution Substances 0.000 description 15
- 239000003446 ligand Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000003112 inhibitor Substances 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 6
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 6
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 6
- 239000003431 cross linking reagent Substances 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 230000019491 signal transduction Effects 0.000 description 5
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 229910001424 calcium ion Inorganic materials 0.000 description 4
- 238000004132 cross linking Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 229920003169 water-soluble polymer Polymers 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- ZLFRJHOBQVVTOJ-UHFFFAOYSA-N dimethyl hexanediimidate Chemical compound COC(=N)CCCCC(=N)OC ZLFRJHOBQVVTOJ-UHFFFAOYSA-N 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- JWDFQMWEFLOOED-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSC1=CC=CC=N1 JWDFQMWEFLOOED-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010007572 Cardiac hypertrophy Diseases 0.000 description 1
- 208000006029 Cardiomegaly Diseases 0.000 description 1
- 229930105110 Cyclosporin A Natural products 0.000 description 1
- PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 description 1
- 108010036949 Cyclosporine Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- QJJXYPPXXYFBGM-LFZNUXCKSA-N Tacrolimus Chemical compound C1C[C@@H](O)[C@H](OC)C[C@@H]1\C=C(/C)[C@@H]1[C@H](C)[C@@H](O)CC(=O)[C@H](CC=C)/C=C(C)/C[C@H](C)C[C@H](OC)[C@H]([C@H](C[C@H]2C)OC)O[C@@]2(O)C(=O)C(=O)N2CCCC[C@H]2C(=O)O1 QJJXYPPXXYFBGM-LFZNUXCKSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 108010025267 calcium-dependent protein kinase Proteins 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005274 electrospray deposition Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002736 metal compounds Chemical class 0.000 description 1
- ADAMEOZKZQRNKP-UHFFFAOYSA-N n'-propylmethanediimine Chemical compound CCCN=C=N ADAMEOZKZQRNKP-UHFFFAOYSA-N 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 201000000980 schizophrenia Diseases 0.000 description 1
- 229960001967 tacrolimus Drugs 0.000 description 1
- QJJXYPPXXYFBGM-SHYZHZOCSA-N tacrolimus Natural products CO[C@H]1C[C@H](CC[C@@H]1O)C=C(C)[C@H]2OC(=O)[C@H]3CCCCN3C(=O)C(=O)[C@@]4(O)O[C@@H]([C@H](C[C@H]4C)OC)[C@@H](C[C@H](C)CC(=C[C@@H](CC=C)C(=O)C[C@H](O)[C@H]2C)C)OC QJJXYPPXXYFBGM-SHYZHZOCSA-N 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N19/00—Investigating materials by mechanical methods
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4727—Calcium binding proteins, e.g. calmodulin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004325005A JP2006133164A (ja) | 2004-11-09 | 2004-11-09 | カルモデュリンの構造変化を検出する方法、カルモデュリンの構造変化に影響を与える活性を有する物質を探索する方法 |
| JP2004-325005 | 2004-11-09 | ||
| PCT/JP2005/020377 WO2006051756A1 (fr) | 2004-11-09 | 2005-11-07 | Procédé de détection de changement de structure de calmoduline, procédé de récupération de matière ayant une activité affectant le changement de structure de calmoduline |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2586415A1 true CA2586415A1 (fr) | 2006-05-18 |
Family
ID=36336437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002586415A Abandoned CA2586415A1 (fr) | 2004-11-09 | 2005-11-07 | Methode de detection de la modification conformationnelle de la calmoduline et methode de criblage de substances dont l'activite a des effets sur la modification conformationnellede la calmoduline |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20080115593A1 (fr) |
| EP (1) | EP1811284A4 (fr) |
| JP (1) | JP2006133164A (fr) |
| CA (1) | CA2586415A1 (fr) |
| WO (1) | WO2006051756A1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101187674B1 (ko) | 2010-09-10 | 2012-10-10 | 송인국 | 이단 면역 크로마토그래피를 이용한 효소 활성 측정법 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2204891T3 (es) * | 1991-04-19 | 2004-05-01 | The Board Of Regents Of The University Of Washington | Adn que codifica fosfodiesterasas de mamiferos. |
| JP3784074B2 (ja) * | 1996-06-20 | 2006-06-07 | ニューヨーク ユニヴァーシティ | ポリマ材料と相互作用するリガンドの検出 |
| JP4433100B2 (ja) * | 1997-06-20 | 2010-03-17 | ニューヨーク ユニヴァーシティ | チップ及びライブラリの大量製造における物質溶液の静電噴霧 |
| US6690463B2 (en) * | 2000-02-10 | 2004-02-10 | Evotec Biosystems Ag | Fluorescence intensity and lifetime distribution analysis |
| AU2001280552A1 (en) * | 2000-07-13 | 2002-01-30 | The Ohio State University Research Foundation | Multimeric biopolymers as structural elements, sensors and actuators in microsystems |
| AU2001292884A1 (en) * | 2000-09-20 | 2002-04-02 | Molecular Reflections | Microfabricated ultrasound array for use as resonant sensors |
| KR100442822B1 (ko) * | 2001-10-23 | 2004-08-02 | 삼성전자주식회사 | 전단응력 측정을 이용한 생분자들간의 결합 여부 검출 방법 |
| CA2463301A1 (fr) * | 2004-04-22 | 2005-10-22 | Atanas Iliev Lalev | Methode de detection d'interactions entre proteines - purification par affinite avec remplacement du ligand |
| JPWO2005093430A1 (ja) * | 2004-03-25 | 2008-02-14 | 株式会社 フューエンス | 蛋白質のアミロイド性の構造変化を検出する方法、アミロイド性の構造変化に影響を与える活性を有する物質を探索する方法、アミロイド関連疾患の治療薬又は診断薬を探索する方法 |
| JP2006090782A (ja) * | 2004-09-22 | 2006-04-06 | Fyuuensu:Kk | 基板上に固定化した蛋白質の構造変化を検出する方法 |
-
2004
- 2004-11-09 JP JP2004325005A patent/JP2006133164A/ja active Pending
-
2005
- 2005-11-07 WO PCT/JP2005/020377 patent/WO2006051756A1/fr active Application Filing
- 2005-11-07 EP EP05800408A patent/EP1811284A4/fr not_active Withdrawn
- 2005-11-07 CA CA002586415A patent/CA2586415A1/fr not_active Abandoned
- 2005-11-07 US US11/666,077 patent/US20080115593A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1811284A1 (fr) | 2007-07-25 |
| WO2006051756A1 (fr) | 2006-05-18 |
| US20080115593A1 (en) | 2008-05-22 |
| EP1811284A4 (fr) | 2008-12-24 |
| JP2006133164A (ja) | 2006-05-25 |
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