CA2522060C - Fragments of fluorescent proteins for protein fragment complementation assays - Google Patents

Fragments of fluorescent proteins for protein fragment complementation assays Download PDF

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Publication number
CA2522060C
CA2522060C CA2522060A CA2522060A CA2522060C CA 2522060 C CA2522060 C CA 2522060C CA 2522060 A CA2522060 A CA 2522060A CA 2522060 A CA2522060 A CA 2522060A CA 2522060 C CA2522060 C CA 2522060C
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gly
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Expired - Fee Related
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CA2522060A
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English (en)
French (fr)
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CA2522060A1 (en
Inventor
Stephen William Watson Michnick
Marnie L. Macdonald
Jane Lamerdin
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Odyssey Pharmaceuticals Inc
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Odyssey Pharmaceuticals Inc
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Publication of CA2522060A1 publication Critical patent/CA2522060A1/en
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Publication of CA2522060C publication Critical patent/CA2522060C/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2522060A 2003-04-09 2004-04-09 Fragments of fluorescent proteins for protein fragment complementation assays Expired - Fee Related CA2522060C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US46113303P 2003-04-09 2003-04-09
US60/461,133 2003-04-09
US10/724,178 US7166424B2 (en) 1998-02-02 2003-12-01 Fragments of fluorescent proteins for protein fragment complementation assays
US10/724,178 2003-12-01
PCT/US2004/010982 WO2004092336A2 (en) 1998-02-02 2004-04-09 Fragments of fluorescent proteins for protein fragment complementation assays

Publications (2)

Publication Number Publication Date
CA2522060A1 CA2522060A1 (en) 2004-10-28
CA2522060C true CA2522060C (en) 2013-10-01

Family

ID=35385090

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2522060A Expired - Fee Related CA2522060C (en) 2003-04-09 2004-04-09 Fragments of fluorescent proteins for protein fragment complementation assays

Country Status (6)

Country Link
US (2) US7166424B2 (https=)
EP (2) EP1616186A4 (https=)
JP (2) JP2006524244A (https=)
AU (2) AU2004230600A1 (https=)
CA (1) CA2522060C (https=)
WO (1) WO2004092336A2 (https=)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020064769A1 (en) * 2000-10-05 2002-05-30 Watson Michnick Stephen William Dynamic visualization of expressed gene networks in living cells
US6897017B1 (en) * 1997-01-31 2005-05-24 Odyssey Thera Inc. Vivo library-versus-library selection of optimized protein-protein interactions
US7062219B2 (en) * 1997-01-31 2006-06-13 Odyssey Thera Inc. Protein fragment complementation assays for high-throughput and high-content screening
US20050221280A1 (en) * 1998-02-02 2005-10-06 Odyssey Thera, Inc. Protein-protein interactions for pharmacological profiling
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
WO2005001115A2 (en) * 2003-05-30 2005-01-06 Odyssey Thera, Inc. Monitoring gene silencing and annotating gene function in living cells
US20070224615A1 (en) * 2003-07-09 2007-09-27 Invitrogen Corporation Methods for assaying protein-protein interactions
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
GB0327143D0 (en) * 2003-11-21 2003-12-24 Univ Belfast Assay
WO2006017279A2 (en) * 2004-07-12 2006-02-16 Board Of Regents, The University Of Texas System Methods and compositions related to identifying protein-protein interactions
US20060094059A1 (en) * 2004-09-22 2006-05-04 Odyssey Thera, Inc. Methods for identifying new drug leads and new therapeutic uses for known drugs
US20060094682A1 (en) 2004-10-29 2006-05-04 Odyssey Thera, Inc. Kinase inhibitors for the treatment of diabetes and obesity
CA2638888A1 (en) * 2004-12-04 2006-06-15 The Regents Of The University Of California Protein subcellular localization assays using split fluorescent proteins
EP1877425A4 (en) * 2005-05-03 2009-04-29 Univ Georgia State Res Found FLUORESCENT PROTEINS AND ITS USE
EP1931787A4 (en) * 2005-09-30 2010-10-20 Univ Montana State SYSTEM FOR DETECTING PROTEIN-PROTEIN INTERACTIONS
KR20080070838A (ko) * 2005-10-27 2008-07-31 트러스티스 오브 보스턴 유니버시티 활성화된 분절­폴리펩티드 및 이의 생산 방법 및 이의 용도
AU2006305901A1 (en) * 2005-10-27 2007-05-03 Trustees Of Boston University Real time nucleic acid detection in vivo using protein complementation
US20090170091A1 (en) * 2006-01-17 2009-07-02 Kenneth Giuliano Method For Predicting Biological Systems Responses
EP1847604A1 (en) 2006-04-19 2007-10-24 Millegen Intracellular method for selecting a peptide or polypeptide which binds to a bait peptide or polypeptide
EP2027465A2 (en) 2006-05-17 2009-02-25 Cellumen, Inc. Method for automated tissue analysis
WO2008060483A2 (en) * 2006-11-10 2008-05-22 Cellumen, Inc. Protein-protein interaction biosensors and methods of use thereof
EP2210104A4 (en) * 2007-11-01 2011-02-23 Univ Arizona State CELL-FREE PROCEDURE FOR DETECTING PROTEIN-LIGAND INTERACTIONS
WO2009111073A2 (en) * 2008-03-06 2009-09-11 Odyssey Thera, Inc. High-content and high throughput assays for identification of lipid-regulating pathways, and novel therapeutic agents for lipid disorders
WO2011135040A1 (en) 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag Fluorescent antibody fusion protein, its production and use
AU2011293349B2 (en) 2010-08-24 2014-04-03 Safewhite, Inc. Methods and materials for providing teeth with a white appearance
US10018631B2 (en) 2011-03-17 2018-07-10 Cernostics, Inc. Systems and compositions for diagnosing Barrett's esophagus and methods of using the same
KR102413041B1 (ko) 2014-01-14 2022-06-23 아세다사이언시즈 아게 기능성 세포 상태의 확인
CA2851568A1 (en) 2014-05-09 2015-11-09 The Governors Of The University Of Alberta Drug discovery and protein-protein interaction assay using flourescent protein exchange
KR101751917B1 (ko) * 2016-02-02 2017-06-28 기초과학연구원 가역적으로 활성화 가능한 항체 유사체 및 그의 용도
CN108267595A (zh) * 2017-11-27 2018-07-10 南京天纵易康生物科技股份有限公司 一种基于双分子荧光互补技术的Myo检测试剂盒、制备及使用方法
CN111718417B (zh) * 2019-03-19 2022-10-14 宁波鲲鹏生物科技有限公司 含有荧光蛋白片段的融合蛋白及其用途
EP4554964A1 (en) * 2022-07-11 2025-05-21 Cornell University Chemically stable fluorescent proteins for advanced microscopy

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US7062219B2 (en) * 1997-01-31 2006-06-13 Odyssey Thera Inc. Protein fragment complementation assays for high-throughput and high-content screening
US6294330B1 (en) * 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
WO2001087919A2 (en) * 2000-05-12 2001-11-22 Yale University Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins
AU2003226955A1 (en) * 2002-04-19 2003-11-03 Bioimage A/S Translocation dependent complementation for drug screening
EP1497325A1 (en) * 2002-04-19 2005-01-19 BioImage A/S Two green fluorescent protein fragments and their use in a method for detecting protein - protein interactions

Also Published As

Publication number Publication date
EP1616186A4 (en) 2007-06-06
US8101364B2 (en) 2012-01-24
CA2522060A1 (en) 2004-10-28
WO2004092336A3 (en) 2005-06-09
US20040137528A1 (en) 2004-07-15
JP2006524244A (ja) 2006-10-26
WO2004092336A2 (en) 2004-10-28
AU2011201031A1 (en) 2011-04-14
EP1616186A2 (en) 2006-01-18
JP2011182796A (ja) 2011-09-22
US20070254373A1 (en) 2007-11-01
EP2317318A1 (en) 2011-05-04
US7166424B2 (en) 2007-01-23
AU2004230600A1 (en) 2004-10-28

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Effective date: 20150409