CA2519702A1 - Method and kit for the specific detection of m.tuberculosis - Google Patents
Method and kit for the specific detection of m.tuberculosis Download PDFInfo
- Publication number
- CA2519702A1 CA2519702A1 CA002519702A CA2519702A CA2519702A1 CA 2519702 A1 CA2519702 A1 CA 2519702A1 CA 002519702 A CA002519702 A CA 002519702A CA 2519702 A CA2519702 A CA 2519702A CA 2519702 A1 CA2519702 A1 CA 2519702A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- tuberculosis
- pair
- sequence
- hybridisation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 201000008827 tuberculosis Diseases 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 56
- 238000011895 specific detection Methods 0.000 title claims abstract description 26
- 241000186366 Mycobacterium bovis Species 0.000 claims abstract description 20
- 241001467552 Mycobacterium bovis BCG Species 0.000 claims abstract description 11
- 241001467553 Mycobacterium africanum Species 0.000 claims abstract description 9
- 239000012472 biological sample Substances 0.000 claims abstract description 6
- 241000187919 Mycobacterium microti Species 0.000 claims abstract description 3
- 239000000523 sample Substances 0.000 claims description 131
- 238000009396 hybridization Methods 0.000 claims description 103
- 108020004414 DNA Proteins 0.000 claims description 72
- 230000000295 complement effect Effects 0.000 claims description 63
- 125000003729 nucleotide group Chemical group 0.000 claims description 60
- 239000002773 nucleotide Substances 0.000 claims description 58
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 46
- 108090000913 Nitrate Reductases Proteins 0.000 claims description 44
- 230000003321 amplification Effects 0.000 claims description 43
- 239000012634 fragment Substances 0.000 claims description 40
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 33
- 238000001514 detection method Methods 0.000 claims description 31
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 24
- 238000003752 polymerase chain reaction Methods 0.000 claims description 23
- 108091081024 Start codon Proteins 0.000 claims description 20
- 238000013519 translation Methods 0.000 claims description 20
- 238000011144 upstream manufacturing Methods 0.000 claims description 20
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 16
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims description 16
- 150000007523 nucleic acids Chemical class 0.000 claims description 16
- 108091034117 Oligonucleotide Proteins 0.000 claims description 14
- 208000015181 infectious disease Diseases 0.000 claims description 12
- 239000012568 clinical material Substances 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000001574 biopsy Methods 0.000 claims description 7
- 210000003608 fece Anatomy 0.000 claims description 7
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- 210000003296 saliva Anatomy 0.000 claims description 7
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- 239000008280 blood Substances 0.000 claims description 6
- 210000001185 bone marrow Anatomy 0.000 claims description 6
- 210000004051 gastric juice Anatomy 0.000 claims description 6
- 241000187479 Mycobacterium tuberculosis Species 0.000 claims description 5
- 239000012752 auxiliary agent Substances 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims 1
- 238000001917 fluorescence detection Methods 0.000 claims 1
- 241001302239 Mycobacterium tuberculosis complex Species 0.000 abstract description 27
- 241001467578 Microbacterium Species 0.000 abstract 4
- 241001314546 Microtis <orchid> Species 0.000 abstract 1
- 244000052769 pathogen Species 0.000 description 29
- 230000001717 pathogenic effect Effects 0.000 description 18
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- 238000012360 testing method Methods 0.000 description 13
- 239000000975 dye Substances 0.000 description 6
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- 238000003745 diagnosis Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical class [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 241000186359 Mycobacterium Species 0.000 description 3
- 206010044756 Tuberculous infections Diseases 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000000692 anti-sense effect Effects 0.000 description 3
- 238000007834 ligase chain reaction Methods 0.000 description 3
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- 239000000463 material Substances 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- -1 naphtyl amide Chemical class 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 238000011309 routine diagnosis Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 241000329526 Guggenheimella bovis Species 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000012505 colouration Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000001004 diazonium dye Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 101150073102 narG gene Proteins 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10313791A DE10313791A1 (de) | 2003-03-20 | 2003-03-20 | Spezifischer Nachweis von Mykobakterien des M. tuberculosis-Komplex |
| DE10313791.2 | 2003-03-20 | ||
| PCT/EP2004/002911 WO2004083459A1 (de) | 2003-03-20 | 2004-03-19 | Verfahren und kit zum spezifischen nachweis von m. tuberculosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2519702A1 true CA2519702A1 (en) | 2004-09-30 |
Family
ID=32946237
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002519702A Abandoned CA2519702A1 (en) | 2003-03-20 | 2004-03-19 | Method and kit for the specific detection of m.tuberculosis |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7749696B2 (enExample) |
| EP (1) | EP1606420B1 (enExample) |
| JP (1) | JP4599346B2 (enExample) |
| AT (1) | ATE518964T1 (enExample) |
| AU (1) | AU2004221678B2 (enExample) |
| CA (1) | CA2519702A1 (enExample) |
| DE (1) | DE10313791A1 (enExample) |
| WO (1) | WO2004083459A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009087688A2 (en) * | 2008-01-04 | 2009-07-16 | Premas Biotech Pvt.Ltd | Method for detection of nucleic acid of mycobacterium and uses thereof |
| US20090311677A1 (en) * | 2008-06-13 | 2009-12-17 | Andrew Reeves | Identification of contaminating bacteria in industrial ethanol fermentations |
| WO2014132263A1 (en) * | 2013-02-27 | 2014-09-04 | Council Of Scientific & Industrial Research | Nitrite-reductase (nirb) as potential anti-tubercular target and a method to detect the severity of tuberculosis disease |
| JP2021119751A (ja) * | 2020-01-30 | 2021-08-19 | 東洋紡株式会社 | 微生物の同定方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6294328B1 (en) * | 1998-06-24 | 2001-09-25 | The Institute For Genomic Research | DNA sequences for strain analysis in Mycobacterium tuberculosis |
| EP1169478A2 (de) * | 1999-03-16 | 2002-01-09 | Hans Konrad Schackert | Verfahren zur identifizierung von organismen durch vergleichende genetische analyse sowie primer und hybridisationssonden zur durchführung des verfahrens |
| US7247430B2 (en) | 2001-08-14 | 2007-07-24 | Institut Pasteur | Compositions and methods for detecting multidrug resistant strains of M. tuberculosis having mutations in genes of the mutT family |
-
2003
- 2003-03-20 DE DE10313791A patent/DE10313791A1/de not_active Withdrawn
-
2004
- 2004-03-19 AU AU2004221678A patent/AU2004221678B2/en not_active Expired
- 2004-03-19 EP EP04721892A patent/EP1606420B1/de not_active Expired - Lifetime
- 2004-03-19 JP JP2006504758A patent/JP4599346B2/ja not_active Expired - Lifetime
- 2004-03-19 WO PCT/EP2004/002911 patent/WO2004083459A1/de not_active Ceased
- 2004-03-19 AT AT04721892T patent/ATE518964T1/de active
- 2004-03-19 CA CA002519702A patent/CA2519702A1/en not_active Abandoned
- 2004-03-19 US US10/549,495 patent/US7749696B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004083459A1 (de) | 2004-09-30 |
| AU2004221678B2 (en) | 2009-04-23 |
| EP1606420B1 (de) | 2011-08-03 |
| US7749696B2 (en) | 2010-07-06 |
| EP1606420A1 (de) | 2005-12-21 |
| JP2006521797A (ja) | 2006-09-28 |
| AU2004221678A1 (en) | 2004-09-30 |
| DE10313791A1 (de) | 2004-10-07 |
| JP4599346B2 (ja) | 2010-12-15 |
| US20070015157A1 (en) | 2007-01-18 |
| ATE518964T1 (de) | 2011-08-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |