CA2485218A1 - Method for creating polynucleotide molecules - Google Patents

Method for creating polynucleotide molecules Download PDF

Info

Publication number
CA2485218A1
CA2485218A1 CA002485218A CA2485218A CA2485218A1 CA 2485218 A1 CA2485218 A1 CA 2485218A1 CA 002485218 A CA002485218 A CA 002485218A CA 2485218 A CA2485218 A CA 2485218A CA 2485218 A1 CA2485218 A1 CA 2485218A1
Authority
CA
Canada
Prior art keywords
polynucleotide molecules
stranded polynucleotide
double
dna
strand breaks
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002485218A
Other languages
English (en)
French (fr)
Inventor
Markus Matuschek
Bernhard Hauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2485218A1 publication Critical patent/CA2485218A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
CA002485218A 2002-05-24 2003-05-21 Method for creating polynucleotide molecules Abandoned CA2485218A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10223057A DE10223057A1 (de) 2002-05-24 2002-05-24 Verfahren zur Erzeugung von Polynukleotidmolekülen
DE10223057.9 2002-05-24
PCT/EP2003/005308 WO2003100058A2 (de) 2002-05-24 2003-05-21 Verfahren zur erzeugung von polynukleotidmolekülen

Publications (1)

Publication Number Publication Date
CA2485218A1 true CA2485218A1 (en) 2003-12-04

Family

ID=29432268

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002485218A Abandoned CA2485218A1 (en) 2002-05-24 2003-05-21 Method for creating polynucleotide molecules

Country Status (9)

Country Link
EP (1) EP1511842A2 (ja)
JP (1) JP2005529596A (ja)
KR (1) KR20050004207A (ja)
CN (1) CN1656221A (ja)
AU (1) AU2003240683A1 (ja)
CA (1) CA2485218A1 (ja)
DE (1) DE10223057A1 (ja)
MX (1) MXPA04011554A (ja)
WO (1) WO2003100058A2 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10927394B2 (en) 2017-01-19 2021-02-23 Oxford Nanopore Technologies Limited Methods and reagents for synthesising polynucleotide molecules

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2422749A1 (en) * 2000-09-21 2002-03-28 Merck & Co., Inc. Method for generating recombinant polynucleotides
WO2002079468A2 (en) * 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10927394B2 (en) 2017-01-19 2021-02-23 Oxford Nanopore Technologies Limited Methods and reagents for synthesising polynucleotide molecules
US11884950B2 (en) 2017-01-19 2024-01-30 Oxford Nanopore Technologies Plc Methods and reagents for synthesising polynucleotide molecules

Also Published As

Publication number Publication date
DE10223057A1 (de) 2003-12-11
JP2005529596A (ja) 2005-10-06
KR20050004207A (ko) 2005-01-12
WO2003100058A3 (de) 2004-09-02
EP1511842A2 (de) 2005-03-09
MXPA04011554A (es) 2005-03-07
AU2003240683A1 (en) 2003-12-12
CN1656221A (zh) 2005-08-17
WO2003100058A2 (de) 2003-12-04

Similar Documents

Publication Publication Date Title
AU783823B2 (en) Method for the production of biopolymers with modified properties
EP2396430B1 (en) Template-independent ligation of single-stranded dna
CN104114702B (zh) 模板转换用于dna合成的用途
Nikoomanzar et al. Engineering polymerases for applications in synthetic biology
EP1817413B1 (en) Oligonucleotide ladder assembly and system for generating molecular diversity
EP2050819A1 (en) Method for amplification of nucleotide sequence
AU2002321251B2 (en) Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
EP1929012A2 (en) Method for in vitro recombination
Wang et al. Mutant library construction in directed molecular evolution: casting a wider net
AU2002321251A1 (en) Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
JPWO2002036822A1 (ja) 核酸塩基配列決定方法
US6641998B2 (en) Methods and kits to enrich for desired nucleic acid sequences
CA2485218A1 (en) Method for creating polynucleotide molecules
Shiba et al. Guide oligonucleotide-dependent DNA linkage that facilitates controllable polymerization of microgene blocks
Adamthwaite Novel Approach to Synthesizing Sticky Ended DNA
Tian-Wen et al. Mutant library construction in directed molecular evolution
CA2486900A1 (en) A method for obtaining circular mutated and/or chimaeric polynucleotides

Legal Events

Date Code Title Description
FZDE Discontinued