CA2467814A1 - Procedes multiplexes de detection de methylation - Google Patents

Procedes multiplexes de detection de methylation Download PDF

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Publication number
CA2467814A1
CA2467814A1 CA002467814A CA2467814A CA2467814A1 CA 2467814 A1 CA2467814 A1 CA 2467814A1 CA 002467814 A CA002467814 A CA 002467814A CA 2467814 A CA2467814 A CA 2467814A CA 2467814 A1 CA2467814 A1 CA 2467814A1
Authority
CA
Canada
Prior art keywords
target
probes
probe
nucleic acid
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002467814A
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English (en)
Inventor
Jian-Bing Fan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2467814A1 publication Critical patent/CA2467814A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention a trait à des dosages multiplexés, sensibles et précis, servant à la détection d'un analysat cible ainsi qu'à la détection d'une méthylation dans des échantillons d'acide nucléique.
CA002467814A 2001-12-03 2002-12-03 Procedes multiplexes de detection de methylation Abandoned CA2467814A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33695801P 2001-12-03 2001-12-03
US60/336,958 2001-12-03
PCT/US2002/038672 WO2003048732A2 (fr) 2001-02-07 2002-12-03 Procedes multiplexes de detection de methylation

Publications (1)

Publication Number Publication Date
CA2467814A1 true CA2467814A1 (fr) 2003-06-12

Family

ID=32823667

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002467814A Abandoned CA2467814A1 (fr) 2001-12-03 2002-12-03 Procedes multiplexes de detection de methylation

Country Status (4)

Country Link
EP (1) EP1461458A4 (fr)
AU (1) AU2002360474A1 (fr)
CA (1) CA2467814A1 (fr)
WO (1) WO2003048732A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1860200A1 (fr) * 2001-12-03 2007-11-28 Illumina, Inc. Procédés multiplexes de détection de methylation
US8150626B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
ATE476523T1 (de) 2003-06-17 2010-08-15 Human Genetic Signatures Pty Verfahren zur genomamplifikation
US7846693B2 (en) 2003-09-04 2010-12-07 Human Genetic Signatures Pty. Ltd. Nucleic acid detection assay
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
EP1794173B1 (fr) 2004-09-10 2010-08-04 Human Genetic Signatures PTY Ltd Bloqueur d'amplification comprenant des acides nucleiques intercalants (ina) contenant des pseudonucleotides intercalants (ipn)
ES2399054T3 (es) 2004-12-03 2013-03-25 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas
US8431347B2 (en) 2005-05-26 2013-04-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
AU2009311769A1 (en) * 2008-11-05 2010-05-14 Stichting Sanquin Bloedvoorziening Means and methods for investigating nucleic acid sequences
EP2753710B1 (fr) 2011-09-07 2017-01-11 Human Genetic Signatures Pty Ltd Essai de détection moléculaire
CN102559661B (zh) * 2012-01-18 2014-06-04 厦门基科生物科技有限公司 一种新型连接酶反应介导的扩增方法及用途

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
EP0238332A2 (fr) * 1986-03-19 1987-09-23 Cetus Corporation Méthode d'hybridation en phase liquide et trousse de réactifs pour détecter la présence de séquences d'acides nucléiques dans des échantillons
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
AU5495194A (en) * 1993-02-26 1994-09-01 Becton Dickinson & Company Flow through membrane nucleic acid hybridization assay
WO1995025538A1 (fr) * 1994-03-18 1995-09-28 The General Hospital Corporation Methodes de detection de polymorphismes amplifies et clives des sites de restriction
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
WO1998056952A1 (fr) * 1997-06-09 1998-12-17 University Of Southern California Methode de diagnostic du cancer basee sur des differences de methylation d'adn
AU8267998A (en) * 1997-06-24 1999-01-04 Trustees Of Boston University High density streptavidin supports
AU8381698A (en) * 1997-07-03 1999-01-25 Case Western Reserve University Compositions and methods for detection of genomic imprinting disorders
US7700324B1 (en) * 1998-11-03 2010-04-20 The Johns Hopkins University School Of Medicine Methylated CpG island amplification (MCA)
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis

Also Published As

Publication number Publication date
WO2003048732A2 (fr) 2003-06-12
WO2003048732A3 (fr) 2003-09-25
EP1461458A4 (fr) 2005-10-26
AU2002360474A1 (en) 2003-06-17
EP1461458A2 (fr) 2004-09-29

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued