WO2003048732A3 - Procedes multiplexes de detection de methylation - Google Patents

Procedes multiplexes de detection de methylation Download PDF

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Publication number
WO2003048732A3
WO2003048732A3 PCT/US2002/038672 US0238672W WO03048732A3 WO 2003048732 A3 WO2003048732 A3 WO 2003048732A3 US 0238672 W US0238672 W US 0238672W WO 03048732 A3 WO03048732 A3 WO 03048732A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection methods
methylation detection
multiplexed
multiplexed methylation
detection
Prior art date
Application number
PCT/US2002/038672
Other languages
English (en)
Other versions
WO2003048732A2 (fr
Inventor
Jian-Bing Fan
Original Assignee
Illumina Inc
Jian-Bing Fan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/779,376 external-priority patent/US20020006617A1/en
Application filed by Illumina Inc, Jian-Bing Fan filed Critical Illumina Inc
Priority to EP02795731A priority Critical patent/EP1461458A4/fr
Priority to CA002467814A priority patent/CA2467814A1/fr
Priority to AU2002360474A priority patent/AU2002360474A1/en
Publication of WO2003048732A2 publication Critical patent/WO2003048732A2/fr
Publication of WO2003048732A3 publication Critical patent/WO2003048732A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention a trait à des dosages multiplexés, sensibles et précis, servant à la détection d'un analysat cible ainsi qu'à la détection d'une méthylation dans des échantillons d'acide nucléique.
PCT/US2002/038672 2001-02-07 2002-12-03 Procedes multiplexes de detection de methylation WO2003048732A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02795731A EP1461458A4 (fr) 2001-12-03 2002-12-03 Procedes multiplexes de detection de methylation
CA002467814A CA2467814A1 (fr) 2001-12-03 2002-12-03 Procedes multiplexes de detection de methylation
AU2002360474A AU2002360474A1 (en) 2001-12-03 2002-12-03 Multiplexed methylation detection methods

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/779,376 US20020006617A1 (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming
US33695801P 2001-12-03 2001-12-03
US60/336,958 2001-12-03

Publications (2)

Publication Number Publication Date
WO2003048732A2 WO2003048732A2 (fr) 2003-06-12
WO2003048732A3 true WO2003048732A3 (fr) 2003-09-25

Family

ID=32823667

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/038672 WO2003048732A2 (fr) 2001-02-07 2002-12-03 Procedes multiplexes de detection de methylation

Country Status (4)

Country Link
EP (1) EP1461458A4 (fr)
AU (1) AU2002360474A1 (fr)
CA (1) CA2467814A1 (fr)
WO (1) WO2003048732A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1860200A1 (fr) * 2001-12-03 2007-11-28 Illumina, Inc. Procédés multiplexes de détection de methylation
US8150626B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
EP1641936B1 (fr) 2003-06-17 2010-08-04 Human Genetic Signatures PTY Ltd. Procedes d'amplification de genome
DE602004018801D1 (de) 2003-09-04 2009-02-12 Human Genetic Signatures Pty Nukleinsäurenachweistest
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
DK1794173T3 (da) 2004-09-10 2010-10-25 Human Genetic Signatures Pty Amplifikationsblokker indeholdende interkalaterende nukleinsyrer (INA) indeholdende interkalaterende pseudonukleotider (IPN)
EP1828411B1 (fr) 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Procedes de simplification d'acides nucleiques microbiens par modification chimique de cytosines
CN101203618B (zh) 2005-05-26 2013-03-13 人类遗传标记控股有限公司 使用含有非常规碱基的引物的等温链置换扩增
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2010053363A1 (fr) * 2008-11-05 2010-05-14 Stichting Sanquin Bloedvoorziening Moyens et procédés pour rechercher des séquences d'acide nucléique
US9732375B2 (en) 2011-09-07 2017-08-15 Human Genetic Signatures Pty. Ltd. Molecular detection assay using direct treatment with a bisulphite reagent
CN102559661B (zh) * 2012-01-18 2014-06-04 厦门基科生物科技有限公司 一种新型连接酶反应介导的扩增方法及用途

Family Cites Families (14)

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CA1256005A (fr) * 1983-09-26 1989-06-20 W. Peter Hansen Methode d'hybridation sandwich pour la detection d'acides nucleiques
AU7015287A (en) * 1986-03-19 1987-09-24 Cetus Corporation Detection of nucleic acid sequence using liquid hybridization method
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
EP0656068B1 (fr) * 1992-07-24 1999-12-15 University Of South Australia Procede d'amplification et de detection
AU5495194A (en) * 1993-02-26 1994-09-01 Becton Dickinson & Company Flow through membrane nucleic acid hybridization assay
WO1995025538A1 (fr) * 1994-03-18 1995-09-28 The General Hospital Corporation Methodes de detection de polymorphismes amplifies et clives des sites de restriction
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
AU7829398A (en) * 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
AU8267998A (en) * 1997-06-24 1999-01-04 Trustees Of Boston University High density streptavidin supports
AU8381698A (en) * 1997-07-03 1999-01-25 Case Western Reserve University Compositions and methods for detection of genomic imprinting disorders
US7700324B1 (en) * 1998-11-03 2010-04-20 The Johns Hopkins University School Of Medicine Methylated CpG island amplification (MCA)
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JONES ET AL.: "Fluorescent and radioactive solid phase dideoxy sequencing of pcr products in microtitre plates", DNA SEQUENCE- J. DNA SEQUENCE AND MAPPING, vol. 1, 1991, pages 279 - 283, XP002967422 *
ROTH ET AL.: "Biotin-avidin microplate assay for the quantitative analysis of enzymatic methylation of DNA by DNA methyltransferases", BIOL. CHEM., vol. 381, March 2000 (2000-03-01), pages 269 - 272, XP002967421 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

Also Published As

Publication number Publication date
EP1461458A4 (fr) 2005-10-26
AU2002360474A1 (en) 2003-06-17
CA2467814A1 (fr) 2003-06-12
WO2003048732A2 (fr) 2003-06-12
EP1461458A2 (fr) 2004-09-29

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