CA2458811C - Selection of cells expressing heteromeric polypeptides - Google Patents

Selection of cells expressing heteromeric polypeptides Download PDF

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Publication number
CA2458811C
CA2458811C CA2458811A CA2458811A CA2458811C CA 2458811 C CA2458811 C CA 2458811C CA 2458811 A CA2458811 A CA 2458811A CA 2458811 A CA2458811 A CA 2458811A CA 2458811 C CA2458811 C CA 2458811C
Authority
CA
Canada
Prior art keywords
nucleic acid
subunit
vector
expression system
selectable marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2458811A
Other languages
English (en)
French (fr)
Other versions
CA2458811A1 (en
Inventor
Jeffrey T. Mcgrew
Allison A. Bianchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunex Corp
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunex Corp filed Critical Immunex Corp
Publication of CA2458811A1 publication Critical patent/CA2458811A1/en
Application granted granted Critical
Publication of CA2458811C publication Critical patent/CA2458811C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2458811A 2001-09-20 2002-09-20 Selection of cells expressing heteromeric polypeptides Expired - Lifetime CA2458811C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32395401P 2001-09-20 2001-09-20
US60/323,954 2001-09-20
PCT/US2002/029985 WO2003035887A1 (en) 2001-09-20 2002-09-20 Selection of cells expressing heteromeric polypeptides

Publications (2)

Publication Number Publication Date
CA2458811A1 CA2458811A1 (en) 2003-05-01
CA2458811C true CA2458811C (en) 2011-05-10

Family

ID=23261429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2458811A Expired - Lifetime CA2458811C (en) 2001-09-20 2002-09-20 Selection of cells expressing heteromeric polypeptides

Country Status (12)

Country Link
US (3) US7691605B2 (lt)
EP (3) EP3467117B1 (lt)
JP (1) JP4266825B2 (lt)
CA (1) CA2458811C (lt)
CY (1) CY1118782T1 (lt)
DK (1) DK1434871T3 (lt)
ES (2) ES2711161T3 (lt)
LT (1) LT1434871T (lt)
MX (1) MXPA04002489A (lt)
PL (1) PL232477B1 (lt)
PT (1) PT1434871T (lt)
WO (1) WO2003035887A1 (lt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3467117B1 (en) * 2001-09-20 2020-07-15 Immunex Corporation Selection of cells expressing heteromeric polypeptides
CA2589860A1 (en) * 2005-01-24 2006-08-03 Amgen Inc. Humanized anti-amyloid antibody
TWI395754B (zh) 2006-04-24 2013-05-11 Amgen Inc 人類化之ｃ-ｋｉｔ抗體
WO2009085200A2 (en) 2007-12-21 2009-07-09 Amgen Inc. Anti-amyloid antibodies and uses thereof
KR101272817B1 (ko) * 2010-04-29 2013-06-10 한국생명공학연구원 항체 생산 세포주 선별 방법 및 선별 키트
MX338953B (es) * 2010-08-16 2016-05-06 Novimmune Sa Metodos para la generacion de anticuerpos multiespecificos y multivalentes.
EP3114226A4 (en) * 2014-03-03 2017-07-26 Novogy Inc. Reducing horizontal gene transfer of functional proteins
MX2018013882A (es) 2016-05-11 2019-03-14 Amgen Inc Seleccion directa de celulas que expresan niveles altos de proteinas heterodimericas usando vectores de complementacion intragenica de glutamina sintetasa.
WO2020056341A2 (en) * 2018-09-14 2020-03-19 Northwestern University Programming protein polymerization with dna
EP3856910A4 (en) * 2018-09-24 2022-09-28 Merck Sharp & Dohme Corp. EXPRESSION VECTORS FOR EUKARYOT EXPRESSION SYSTEMS
WO2020227206A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545503A (en) * 1980-06-25 1996-08-13 Semiconductor Energy Laboratory Co., Ltd. Method of making printing member for electrostatic photocopying
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
AU643427B2 (en) * 1988-10-31 1993-11-18 Immunex Corporation Interleukin-4 receptors
IL162181A (en) 1988-12-28 2006-04-10 Pdl Biopharma Inc A method of producing humanized immunoglubulin, and polynucleotides encoding the same
DE69233482T2 (de) 1991-05-17 2006-01-12 Merck & Co., Inc. Verfahren zur Verminderung der Immunogenität der variablen Antikörperdomänen
US5624818A (en) 1991-09-09 1997-04-29 Fred Hutchinson Cancer Research Center Nucleic acids encoding regulatory proteins that dimerize with Mad or Max
DK0822199T3 (da) 1991-10-25 2004-12-27 Amgen Inc N-terminalt monopegylerede polypeptider og fremgangsmåder til fremstilling heraf
US6129914A (en) * 1992-03-27 2000-10-10 Protein Design Labs, Inc. Bispecific antibody effective to treat B-cell lymphoma and cell line
US5928904A (en) * 1993-09-07 1999-07-27 Smithkline Beecham Corporation DNA encoding recombinant IL4 antibodies useful in treatment of IL4 mediated disorders
US5573925A (en) 1994-11-28 1996-11-12 The Wistar Institute Of Anatomy And Biology P53 proteins with altered tetramerization domains
AU693713B2 (en) 1995-06-07 1998-07-02 Immunex Corporation CD40L mutein
AU4596597A (en) * 1996-09-27 1998-04-17 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US5916771A (en) 1996-10-11 1999-06-29 Abgenix, Inc. Production of a multimeric protein by cell fusion method
US6294330B1 (en) * 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
DE60024659T2 (de) * 1999-10-13 2006-08-17 Immunex Corp., Thousand Oaks Vektoren und verfahren für rekombinante proteinexpression
AUPQ422399A0 (en) * 1999-11-24 1999-12-16 University Of New South Wales, The Method of screening transformed or transfected cells
EP3467117B1 (en) * 2001-09-20 2020-07-15 Immunex Corporation Selection of cells expressing heteromeric polypeptides

Also Published As

Publication number Publication date
EP3467117A1 (en) 2019-04-10
US20030082735A1 (en) 2003-05-01
EP1434871A4 (en) 2004-11-03
PL370306A1 (en) 2005-05-16
JP4266825B2 (ja) 2009-05-20
EP1434871A1 (en) 2004-07-07
US7691605B2 (en) 2010-04-06
JP2005511031A (ja) 2005-04-28
LT1434871T (lt) 2017-04-10
EP3187592A1 (en) 2017-07-05
MXPA04002489A (es) 2004-11-22
EP3467117B1 (en) 2020-07-15
US7968313B2 (en) 2011-06-28
PT1434871T (pt) 2017-04-05
DK1434871T3 (en) 2017-04-18
US20070031422A1 (en) 2007-02-08
WO2003035887A1 (en) 2003-05-01
PL232477B1 (pl) 2019-06-28
CY1118782T1 (el) 2017-07-12
ES2620410T3 (es) 2017-06-28
ES2711161T3 (es) 2019-04-30
CA2458811A1 (en) 2003-05-01
US20100055794A1 (en) 2010-03-04
EP3187592B1 (en) 2018-12-12
EP1434871B1 (en) 2017-01-18

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Effective date: 20220920