CA2457163A1

CA2457163A1 - Derp1 and proderp1 allergen derivatives

Derp1 and proderp1 allergen derivatives Download PDF

Info

Publication number: CA2457163A1
Authority: CA; Canada
Prior art keywords: ala; ile; asn; tyr; arg
Prior art date: 2001-08-17
Legal status : Abandoned

Application number

CA002457163A

Other languages

English (en)

French (fr)

Inventor

Alex Bollen

Alain Jacquet

Mauro Magi

Remi M. Palmantier

Current Assignee

GlaxoSmithKline Biologicals SA

Original Assignee

Individual

Priority date

2001-08-17

Filing date

2002-08-15

Publication date

2003-02-27

2002-08-15 Application filed by Individual filed Critical Individual

2003-02-27 Publication of CA2457163A1 publication Critical patent/CA2457163A1/en

Status Abandoned legal-status Critical Current

Links

239000013566 allergen Substances 0.000 title claims abstract description 74
208000026935 allergic disease Diseases 0.000 claims abstract description 25
206010020751 Hypersensitivity Diseases 0.000 claims abstract description 16
230000007815 allergy Effects 0.000 claims abstract description 14
230000028993 immune response Effects 0.000 claims abstract description 14
238000011282 treatment Methods 0.000 claims abstract description 8
108090000623 proteins and genes Proteins 0.000 claims description 107
102000004169 proteins and genes Human genes 0.000 claims description 81
235000018102 proteins Nutrition 0.000 claims description 78
230000035772 mutation Effects 0.000 claims description 38
239000000203 mixture Substances 0.000 claims description 34
238000000034 method Methods 0.000 claims description 31
239000002671 adjuvant Substances 0.000 claims description 28
150000007523 nucleic acids Chemical class 0.000 claims description 24
239000013598 vector Substances 0.000 claims description 23
102000039446 nucleic acids Human genes 0.000 claims description 19
108020004707 nucleic acids Proteins 0.000 claims description 19
108700010070 Codon Usage Proteins 0.000 claims description 16
239000013604 expression vector Substances 0.000 claims description 16
102000040430 polynucleotide Human genes 0.000 claims description 16
108091033319 polynucleotide Proteins 0.000 claims description 16
239000002157 polynucleotide Substances 0.000 claims description 16
238000004519 manufacturing process Methods 0.000 claims description 15
230000002009 allergenic effect Effects 0.000 claims description 13
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 12
230000002163 immunogen Effects 0.000 claims description 12
235000018417 cysteine Nutrition 0.000 claims description 11
230000002829 reductive effect Effects 0.000 claims description 11
108091034117 Oligonucleotide Proteins 0.000 claims description 9
108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
-1 polyethylene Polymers 0.000 claims description 5
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 3
102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
239000004698 Polyethylene Substances 0.000 claims description 2
150000002148 esters Chemical class 0.000 claims description 2
229920000573 polyethylene Polymers 0.000 claims description 2
239000007762 w/o emulsion Substances 0.000 claims description 2
239000003814 drug Substances 0.000 claims 2
208000010668 atopic eczema Diseases 0.000 abstract description 14
230000000172 allergic effect Effects 0.000 abstract description 13
230000000774 hypoallergenic effect Effects 0.000 abstract description 12
239000008194 pharmaceutical composition Substances 0.000 abstract description 5
108020004414 DNA Proteins 0.000 description 56
210000004027 cell Anatomy 0.000 description 45
KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 37
NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 33
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 31
239000002953 phosphate buffered saline Substances 0.000 description 31
108020004705 Codon Proteins 0.000 description 29
239000013612 plasmid Substances 0.000 description 27
239000000872 buffer Substances 0.000 description 24
230000027455 binding Effects 0.000 description 23
239000012634 fragment Substances 0.000 description 23
229960004784 allergens Drugs 0.000 description 22
230000009257 reactivity Effects 0.000 description 22
CCQOOWAONKGYKQ-BYPYZUCNSA-N Gly-Gly-Ala Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)CN CCQOOWAONKGYKQ-BYPYZUCNSA-N 0.000 description 19
YIBOAHAOAWACDK-QEJZJMRPSA-N Lys-Ala-Phe Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 YIBOAHAOAWACDK-QEJZJMRPSA-N 0.000 description 19
241000699670 Mus sp. Species 0.000 description 19
229960005486 vaccine Drugs 0.000 description 19
ATABBWFGOHKROJ-GUBZILKMSA-N Arg-Pro-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O ATABBWFGOHKROJ-GUBZILKMSA-N 0.000 description 18
108010005233 alanylglutamic acid Proteins 0.000 description 18
KFAFUJMGHVVYRC-DCAQKATOSA-N Asp-Leu-Met Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(O)=O KFAFUJMGHVVYRC-DCAQKATOSA-N 0.000 description 17
KXRORHJIRAOQPG-SOUVJXGZSA-N Glu-Tyr-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CC=C(C=C2)O)NC(=O)[C@H](CCC(=O)O)N)C(=O)O KXRORHJIRAOQPG-SOUVJXGZSA-N 0.000 description 17
QGRJTULYDZUBAY-ZPFDUUQYSA-N Met-Ile-Glu Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(O)=O QGRJTULYDZUBAY-ZPFDUUQYSA-N 0.000 description 17
NZRUWPIYECBYRK-HTUGSXCWSA-N Thr-Phe-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(O)=O)C(O)=O NZRUWPIYECBYRK-HTUGSXCWSA-N 0.000 description 17
GPLTZEMVOCZVAV-UFYCRDLUSA-N Tyr-Tyr-Arg Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)C1=CC=C(O)C=C1 GPLTZEMVOCZVAV-UFYCRDLUSA-N 0.000 description 17
108010013835 arginine glutamate Proteins 0.000 description 17
108010045126 glycyl-tyrosyl-glycine Proteins 0.000 description 17
229960001340 histamine Drugs 0.000 description 17
ZNZPKVQURDQFFS-FXQIFTODSA-N Gln-Glu-Ser Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O ZNZPKVQURDQFFS-FXQIFTODSA-N 0.000 description 16
MFYLRRCYBBJYPI-JYJNAYRXSA-N Glu-Tyr-Lys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N)O MFYLRRCYBBJYPI-JYJNAYRXSA-N 0.000 description 16
KSOBNUBCYHGUKH-UWVGGRQHSA-N Gly-Val-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)CN KSOBNUBCYHGUKH-UWVGGRQHSA-N 0.000 description 16
DNEJSAIMVANNPA-DCAQKATOSA-N Lys-Asn-Arg Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O DNEJSAIMVANNPA-DCAQKATOSA-N 0.000 description 16
JIPVNVNKXJLFJF-BJDJZHNGSA-N Ser-Ile-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CO)N JIPVNVNKXJLFJF-BJDJZHNGSA-N 0.000 description 16
210000001744 T-lymphocyte Anatomy 0.000 description 16
VTMGKRABARCZAX-OSUNSFLBSA-N Thr-Pro-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)[C@@H](C)O VTMGKRABARCZAX-OSUNSFLBSA-N 0.000 description 16
239000000427 antigen Substances 0.000 description 16
108091007433 antigens Proteins 0.000 description 16
102000036639 antigens Human genes 0.000 description 16
238000009472 formulation Methods 0.000 description 16
SFNFGFDRYJKZKN-XQXXSGGOSA-N Ala-Gln-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](C)N)O SFNFGFDRYJKZKN-XQXXSGGOSA-N 0.000 description 15
108090000695 Cytokines Proteins 0.000 description 15
102000004127 Cytokines Human genes 0.000 description 15
KAXZXLSXFWSNNZ-XVYDVKMFSA-N His-Ser-Ala Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O KAXZXLSXFWSNNZ-XVYDVKMFSA-N 0.000 description 15
IIXDMJNYALIKGP-DJFWLOJKSA-N Ile-Asn-His Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N IIXDMJNYALIKGP-DJFWLOJKSA-N 0.000 description 15
150000001413 amino acids Chemical group 0.000 description 15
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 15
108010055341 glutamyl-glutamic acid Proteins 0.000 description 15
RFXXUWGNVRJTNQ-QXEWZRGKSA-N Arg-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)CNC(=O)[C@H](CCCN=C(N)N)N RFXXUWGNVRJTNQ-QXEWZRGKSA-N 0.000 description 14
HJTSRYLPAYGEEC-SIUGBPQLSA-N Glu-Tyr-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)[C@H](CCC(=O)O)N HJTSRYLPAYGEEC-SIUGBPQLSA-N 0.000 description 14
WBPFYNYTYASCQP-CYDGBPFRSA-N Val-Val-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)N WBPFYNYTYASCQP-CYDGBPFRSA-N 0.000 description 14
108010047506 alanyl-glutaminyl-glycyl-valine Proteins 0.000 description 14
LYILPUNCKACNGF-NAKRPEOUSA-N Ala-Val-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)N LYILPUNCKACNGF-NAKRPEOUSA-N 0.000 description 13
HIMXTOIXVXWHTB-DCAQKATOSA-N Arg-Met-Gln Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N HIMXTOIXVXWHTB-DCAQKATOSA-N 0.000 description 13
MQFGXJNSUJTXDT-QSFUFRPTSA-N Ile-Gly-Ile Chemical compound N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)O MQFGXJNSUJTXDT-QSFUFRPTSA-N 0.000 description 13
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LWUWMHIOBPTZBA-DCAQKATOSA-N Ala-Arg-Lys Chemical compound NC(=N)NCCC[C@H](NC(=O)[C@@H](N)C)C(=O)N[C@@H](CCCCN)C(O)=O LWUWMHIOBPTZBA-DCAQKATOSA-N 0.000 description 12
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