CA2430192C - Enzymes having dehalogenase activity and methods of use thereof - Google Patents

Enzymes having dehalogenase activity and methods of use thereof Download PDF

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Publication number
CA2430192C
CA2430192C CA2430192A CA2430192A CA2430192C CA 2430192 C CA2430192 C CA 2430192C CA 2430192 A CA2430192 A CA 2430192A CA 2430192 A CA2430192 A CA 2430192A CA 2430192 C CA2430192 C CA 2430192C
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CA
Canada
Prior art keywords
polypeptide
sequence
sequences
polynucleotide
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2430192A
Other languages
English (en)
French (fr)
Other versions
CA2430192A1 (en
Inventor
Jay M. Short
Toby Richardson
Dan E. Robertson
Kevin A. Gray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
BASF Enzymes LLC
Verenium Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF Enzymes LLC, Verenium Corp filed Critical BASF Enzymes LLC
Priority to CA2846761A priority Critical patent/CA2846761A1/en
Publication of CA2430192A1 publication Critical patent/CA2430192A1/en
Application granted granted Critical
Publication of CA2430192C publication Critical patent/CA2430192C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S422/00Chemical apparatus and process disinfecting, deodorizing, preserving, or sterilizing
    • Y10S422/90Decreasing pollution or environmental impact

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medical Informatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Processing Of Solid Wastes (AREA)
CA2430192A 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof Expired - Fee Related CA2430192C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2846761A CA2846761A1 (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25089700P 2000-12-01 2000-12-01
US60/250,897 2000-12-01
PCT/US2001/045337 WO2002068583A2 (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2846761A Division CA2846761A1 (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof

Publications (2)

Publication Number Publication Date
CA2430192A1 CA2430192A1 (en) 2002-09-06
CA2430192C true CA2430192C (en) 2014-06-03

Family

ID=22949600

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2430192A Expired - Fee Related CA2430192C (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof
CA2846761A Abandoned CA2846761A1 (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2846761A Abandoned CA2846761A1 (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof

Country Status (9)

Country Link
US (4) US7078504B2 (OSRAM)
EP (1) EP1507870A4 (OSRAM)
JP (4) JP4589602B2 (OSRAM)
KR (1) KR20030051897A (OSRAM)
CN (2) CN102690799A (OSRAM)
BR (1) BR0115875A (OSRAM)
CA (2) CA2430192C (OSRAM)
MX (1) MXPA03004840A (OSRAM)
WO (1) WO2002068583A2 (OSRAM)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109706139A (zh) * 2019-03-01 2019-05-03 中国人民解放军92609部队 脱卤素酶基因LinB、脱卤素酶、脱卤素酶基因工程菌及其构建方法和应用方法

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000040715A2 (en) * 1999-01-05 2000-07-13 Trustees Of Boston University Improved nucleic acid cloning
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US7435562B2 (en) * 2000-07-21 2008-10-14 Modular Genetics, Inc. Modular vector systems
EP1507870A4 (en) * 2000-12-01 2005-11-09 Diversa Corp ENZYMS WITH DEHALOGENASE ACTIVITY AND METHOD FOR THEIR USE
WO2004027075A2 (en) * 2002-09-20 2004-04-01 Diversa Corporation Chemoenzymatic methods for the synthesis of statins and stain intermediates
AU2004211584B2 (en) 2003-01-31 2009-11-12 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) * 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
JP2008519602A (ja) 2004-11-11 2008-06-12 モジュラー ジェネティクス, インコーポレイテッド 多様性を生じるためのラダーアセンブリおよびシステム
US7632666B2 (en) * 2004-12-27 2009-12-15 Masarykova Univerzita V Brne Method of production of optically active halohydrocarbons and alcohols using hydrolytic dehalogenation catalysed by haloalkane dehalogenases
WO2008054821A2 (en) * 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2011137368A2 (en) 2010-04-30 2011-11-03 Life Technologies Corporation Systems and methods for analyzing nucleic acid sequences
US9268903B2 (en) 2010-07-06 2016-02-23 Life Technologies Corporation Systems and methods for sequence data alignment quality assessment
CN102296046B (zh) * 2011-09-16 2013-08-14 广州市暨源生物科技有限公司 可用于预防慢性重金属中毒的重组食品级乳酸菌、其制备方法与应用
PL3107554T3 (pl) * 2014-02-19 2020-12-28 University Of Tennessee Research Foundation Przeciwciało dla odchylenia proporcji płci i sposoby jego zastosowania
CN104694514B (zh) * 2015-03-18 2017-11-28 中国科学院微生物研究所 一种脱卤酶DhmB及其编码基因与应用
US10344286B2 (en) 2015-05-13 2019-07-09 Samsung Electronics Co., Ltd. Microorganism including gene encoding protein having hydroxylase activity and method of reducing concentration of fluorinated methane in sample using the same
US10358632B2 (en) 2015-12-07 2019-07-23 Samsung Electronics Co., Ltd. Bacterial cytochrome P450 protein variant and method of reducing concentration of fluorinated methane in sample using the same
CN114574360B (zh) * 2020-12-01 2025-02-11 中国科学院大连化学物理研究所 基于石英内衬管阵列的细胞操作装置及方法
US11760793B2 (en) 2020-12-18 2023-09-19 University Of Tennessee Research Foundation Antibody for skewing sex ratio and methods of use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US6037465A (en) * 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
AU6324998A (en) * 1997-02-13 1998-09-08 Dow Chemical Company, The Recombinant haloaliphatic dehalogenases
US6183740B1 (en) * 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
WO2000018909A2 (en) * 1998-09-29 2000-04-06 Diversa Corporation NUCLEIC ACIDS AND PROTEINS FROM $i(CENARCHAEUM SYMBIOSUM)
AU4039400A (en) * 1999-03-26 2000-10-16 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
JP2004524811A (ja) 2000-09-15 2004-08-19 ディバーサ コーポレーション 有機体の混合集団の組み合わせスクリーニング
EP1507870A4 (en) 2000-12-01 2005-11-09 Diversa Corp ENZYMS WITH DEHALOGENASE ACTIVITY AND METHOD FOR THEIR USE

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109706139A (zh) * 2019-03-01 2019-05-03 中国人民解放军92609部队 脱卤素酶基因LinB、脱卤素酶、脱卤素酶基因工程菌及其构建方法和应用方法

Also Published As

Publication number Publication date
MXPA03004840A (es) 2008-10-08
CN1610754A (zh) 2005-04-27
US7078504B2 (en) 2006-07-18
JP2010268796A (ja) 2010-12-02
JP2008142089A (ja) 2008-06-26
CN102690799A (zh) 2012-09-26
KR20030051897A (ko) 2003-06-25
US20060035216A1 (en) 2006-02-16
WO2002068583A2 (en) 2002-09-06
EP1507870A2 (en) 2005-02-23
US9453211B2 (en) 2016-09-27
CA2430192A1 (en) 2002-09-06
JP2005501515A (ja) 2005-01-20
US20100112666A1 (en) 2010-05-06
CA2846761A1 (en) 2002-09-06
CN1610754B (zh) 2012-07-04
US20130115675A1 (en) 2013-05-09
JP4589602B2 (ja) 2010-12-01
US8153411B2 (en) 2012-04-10
JP2015107123A (ja) 2015-06-11
BR0115875A (pt) 2005-05-10
WO2002068583A3 (en) 2004-12-02
EP1507870A4 (en) 2005-11-09
US7671189B2 (en) 2010-03-02
JP5697365B2 (ja) 2015-04-08
US20060275804A1 (en) 2006-12-07

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Effective date: 20161130