CA2414102A1 - Universal collection medium - Google Patents
Universal collection medium Download PDFInfo
- Publication number
- CA2414102A1 CA2414102A1 CA002414102A CA2414102A CA2414102A1 CA 2414102 A1 CA2414102 A1 CA 2414102A1 CA 002414102 A CA002414102 A CA 002414102A CA 2414102 A CA2414102 A CA 2414102A CA 2414102 A1 CA2414102 A1 CA 2414102A1
- Authority
- CA
- Canada
- Prior art keywords
- medium
- cell
- analysis
- cells
- fixative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 claims abstract description 54
- 238000007479 molecular analysis Methods 0.000 claims abstract description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 40
- 238000004458 analytical method Methods 0.000 claims description 38
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 35
- -1 poly(ethylene Glycol) Polymers 0.000 claims description 32
- 239000000834 fixative Substances 0.000 claims description 25
- 102000004169 proteins and genes Human genes 0.000 claims description 24
- 230000002380 cytological effect Effects 0.000 claims description 22
- 229920001223 polyethylene glycol Polymers 0.000 claims description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 238000006731 degradation reaction Methods 0.000 claims description 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 13
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 239000002738 chelating agent Substances 0.000 claims description 11
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- 239000000872 buffer Substances 0.000 claims description 7
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- 238000004445 quantitative analysis Methods 0.000 claims description 7
- 239000003755 preservative agent Substances 0.000 claims description 6
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 5
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- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical class CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 claims description 3
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- HLVXFWDLRHCZEI-UHFFFAOYSA-N chromotropic acid Chemical compound OS(=O)(=O)C1=CC(O)=C2C(O)=CC(S(O)(=O)=O)=CC2=C1 HLVXFWDLRHCZEI-UHFFFAOYSA-N 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- LJYRLGOJYKPILZ-UHFFFAOYSA-N murexide Chemical compound [NH4+].N1C(=O)NC(=O)C(N=C2C(NC(=O)NC2=O)=O)=C1[O-] LJYRLGOJYKPILZ-UHFFFAOYSA-N 0.000 claims description 3
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 2
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical class CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 2
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- 238000012511 carbohydrate analysis Methods 0.000 claims 1
- MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 claims 1
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- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical group [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 230000001413 cellular effect Effects 0.000 description 12
- 208000022361 Human papillomavirus infectious disease Diseases 0.000 description 11
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- ADKOXSOCTOWDOP-UHFFFAOYSA-L magnesium;aluminum;dihydroxide;trihydrate Chemical compound O.O.O.[OH-].[OH-].[Mg+2].[Al] ADKOXSOCTOWDOP-UHFFFAOYSA-L 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 210000004940 nucleus Anatomy 0.000 description 6
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 6
- 238000002560 therapeutic procedure Methods 0.000 description 6
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- YXANJHQJDJLGLP-UHFFFAOYSA-M sodium;hydrogen sulfate;pentanedial Chemical compound [Na+].OS([O-])(=O)=O.O=CCCCC=O YXANJHQJDJLGLP-UHFFFAOYSA-M 0.000 description 5
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- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
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- 108090000790 Enzymes Proteins 0.000 description 2
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- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
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- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
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- 229910021645 metal ion Inorganic materials 0.000 description 2
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
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- 229950000964 pepstatin Drugs 0.000 description 2
- 108010091212 pepstatin Proteins 0.000 description 2
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 description 2
- YVGZCLMIVQGEPB-UHFFFAOYSA-N 1,5-dihydroxypentane-1,5-disulfonic acid Chemical group OS(=O)(=O)C(O)CCCC(O)S(O)(=O)=O YVGZCLMIVQGEPB-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 206010061041 Chlamydial infection Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000009465 Growth Factor Receptors Human genes 0.000 description 1
- 108010009202 Growth Factor Receptors Proteins 0.000 description 1
- 241000701806 Human papillomavirus Species 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
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- 108010059712 Pronase Proteins 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
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- 108020004518 RNA Probes Proteins 0.000 description 1
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- 241000700584 Simplexvirus Species 0.000 description 1
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- 230000001594 aberrant effect Effects 0.000 description 1
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- 235000011054 acetic acid Nutrition 0.000 description 1
- 150000001243 acetic acids Chemical class 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229940126575 aminoglycoside Drugs 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
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- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
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- LSGOVYNHVSXFFJ-UHFFFAOYSA-N vanadate(3-) Chemical class [O-][V]([O-])([O-])=O LSGOVYNHVSXFFJ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Developing Agents For Electrophotography (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Pens And Brushes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US59857100A | 2000-06-21 | 2000-06-21 | |
| US09/598,571 | 2000-06-21 | ||
| PCT/US2001/041091 WO2001098542A2 (en) | 2000-06-21 | 2001-06-21 | Universal collection medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2414102A1 true CA2414102A1 (en) | 2001-12-27 |
Family
ID=24396098
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002414102A Abandoned CA2414102A1 (en) | 2000-06-21 | 2001-06-21 | Universal collection medium |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7371518B2 (enExample) |
| EP (1) | EP1294853B1 (enExample) |
| JP (1) | JP2012034699A (enExample) |
| AT (1) | ATE466930T1 (enExample) |
| AU (3) | AU1548802A (enExample) |
| BR (1) | BR0112385A (enExample) |
| CA (1) | CA2414102A1 (enExample) |
| DE (1) | DE60142048D1 (enExample) |
| WO (1) | WO2001098542A2 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9814271A (pt) * | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
| US20040115692A1 (en) * | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
| US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| ES2784011T3 (es) | 2002-10-16 | 2020-09-21 | Streck Inc | Procedimiento y dispositivo para recoger y preservar las células para su análisis |
| EP1605244A1 (en) * | 2004-06-09 | 2005-12-14 | Boon, Mathilde Elisabeth | Fixative composition |
| WO2009066695A1 (ja) | 2007-11-20 | 2009-05-28 | Olympus Corporation | 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット |
| AU2009238247B2 (en) * | 2008-04-17 | 2014-12-11 | Qiagen Gaithersburg, Inc. | Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid |
| CN102027132A (zh) | 2008-05-12 | 2011-04-20 | 奥林巴斯株式会社 | 粪便处理方法及粪便处理容器 |
| TWI417389B (zh) | 2008-10-27 | 2013-12-01 | Qiagen Gaithersburg Inc | 於自動平台上之快速結果雜交捕捉法 |
| MX2011007624A (es) * | 2009-01-19 | 2011-10-12 | Hospices Civils Lyon | Metodos para determinar la susceptibilidad de contraer una infeccion nosocomial en el paciente y para establecer el pronostico de la evolucion del sindrome septico. |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| EP3290530B1 (en) * | 2009-02-18 | 2020-09-02 | Streck Inc. | Preservation of cell-free nucleic acids |
| AU2010242867B2 (en) | 2009-05-01 | 2016-05-12 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of RNA splice-forms in a sample |
| JP5826752B2 (ja) | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
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| US133972A (en) * | 1872-12-17 | Improvement in bolt-trimmers | ||
| US4493821A (en) * | 1982-02-04 | 1985-01-15 | Harrison James S | Preservative and fixative preparations for biological systems |
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| DE60142048D1 (de) | 2010-06-17 |
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| US7371518B2 (en) | 2008-05-13 |
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| ATE466930T1 (de) | 2010-05-15 |
| US20030119049A1 (en) | 2003-06-26 |
| AU1548802A (en) | 2002-01-02 |
| BR0112385A (pt) | 2003-06-17 |
| WO2001098542A3 (en) | 2002-06-20 |
| AU2007203372A1 (en) | 2007-08-09 |
| AU2002215488B2 (en) | 2007-04-19 |
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