CA2390639C - Procede et composition permettant de preparer un produit a base de lignocellulose et produit ainsi obtenu - Google Patents
Procede et composition permettant de preparer un produit a base de lignocellulose et produit ainsi obtenu Download PDFInfo
- Publication number
- CA2390639C CA2390639C CA002390639A CA2390639A CA2390639C CA 2390639 C CA2390639 C CA 2390639C CA 002390639 A CA002390639 A CA 002390639A CA 2390639 A CA2390639 A CA 2390639A CA 2390639 C CA2390639 C CA 2390639C
- Authority
- CA
- Canada
- Prior art keywords
- plant
- lignocellulose
- phenolic
- reaction medium
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B15/00—Preparation of other cellulose derivatives or modified cellulose, e.g. complexes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B15/00—Preparation of other cellulose derivatives or modified cellulose, e.g. complexes
- C08B15/10—Crosslinking of cellulose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B31/00—Preparation of derivatives of starch
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
- D06M15/15—Proteins or derivatives thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/001—Modification of pulp properties
- D21C9/002—Modification of pulp properties by chemical means; preparation of dewatered pulp, e.g. in sheet or bulk form, containing special additives
- D21C9/005—Modification of pulp properties by chemical means; preparation of dewatered pulp, e.g. in sheet or bulk form, containing special additives organic compounds
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
- D21H17/005—Microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Textile Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Paper (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne un procédé de fabrication d'un produit à base de lignocellulose. Ce procédé comprend les étapes consistant à mélanger dans un milieu de réaction (i) un polymère phénolique étant substitué par un groupe hydroxy phénolique; (ii) une lignocellulose présentant immobilisé, par rapport à une fraction cellulosique, un polypeptide hybride, ce polymère comprenant une enzyme capable de catalyser l'oxydation des groupes phénoliques ainsi qu'un peptide liant la cellulose; et (iii) un agent d'oxydation. L'invention concerne également une composition à utiliser dans le procédé et un produit à base de lignocellulose qui peut être obtenu au moyen de ce procédé.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16414099P | 1999-11-08 | 1999-11-08 | |
US60/164,140 | 1999-11-08 | ||
US16638999P | 1999-11-18 | 1999-11-18 | |
US60/166,389 | 1999-11-18 | ||
PCT/IL2000/000665 WO2001034902A2 (fr) | 1999-11-08 | 2000-10-19 | Procede et composition permettant de preparer un produit a base de lignocellulose et produit ainsi obtenu |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2390639A1 CA2390639A1 (fr) | 2001-05-17 |
CA2390639C true CA2390639C (fr) | 2009-04-28 |
Family
ID=26860299
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002390639A Expired - Fee Related CA2390639C (fr) | 1999-11-08 | 2000-10-19 | Procede et composition permettant de preparer un produit a base de lignocellulose et produit ainsi obtenu |
CA002390568A Abandoned CA2390568A1 (fr) | 1999-11-08 | 2000-11-02 | Modification de la polysaccharide contenant des materiaux |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002390568A Abandoned CA2390568A1 (fr) | 1999-11-08 | 2000-11-02 | Modification de la polysaccharide contenant des materiaux |
Country Status (9)
Country | Link |
---|---|
US (2) | US20050257905A1 (fr) |
EP (1) | EP1230374A4 (fr) |
JP (2) | JP2003533173A (fr) |
CN (1) | CN1329516C (fr) |
AU (3) | AU776069B2 (fr) |
BR (2) | BR0015519A (fr) |
CA (2) | CA2390639C (fr) |
WO (2) | WO2001034902A2 (fr) |
ZA (1) | ZA200204537B (fr) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003533173A (ja) * | 1999-11-08 | 2003-11-11 | シービーデイ テクノロジーズ リミテッド | リグノセルロース系製品を調製するための方法および組成物ならびに該方法によって得られる製品 |
GB2376017A (en) * | 2000-12-20 | 2002-12-04 | Unilever Plc | Cellulose binding domain conjugates linked or coupled to a polysaccharide backbone |
FI116198B (fi) | 2001-07-16 | 2005-10-14 | Valtion Teknillinen | Polypeptidien immobilisointimenetelmä |
GB0425102D0 (en) | 2004-11-15 | 2004-12-15 | Ciba Spec Chem Water Treat Ltd | Polymeric compositions and methods of employing them in papermaking processes |
EP1833918B1 (fr) * | 2004-12-23 | 2011-09-28 | Dow Corning Corporation | Compositions saccharide-siloxane reticulables, et reseaux, revetements et articles obtenus a partir de celles-ci |
CA2607827C (fr) * | 2005-05-04 | 2013-11-19 | Novozymes North America, Inc. | Compositions et procedes de traitement au dioxyde de chlorure |
WO2007115723A2 (fr) * | 2006-04-06 | 2007-10-18 | Institut Français Du Petrole | Proteines resultant de la fusion entre des enzymes degradant la paroi cellulaire de plantes et une swollenine et leurs utilisations |
GB0609477D0 (en) * | 2006-05-12 | 2006-06-21 | Ciba Sc Holding Ag | Process for making paper and paperboard |
CA2669951A1 (fr) * | 2006-12-04 | 2008-06-12 | Innovative Purification Technologies Pty Ltd | Particules proteiques |
EP2599790A1 (fr) | 2007-11-26 | 2013-06-05 | Yissum Research Development Company of The Hebrew University of Jerusalem | Compositions comprenant des polypeptides fibreux et des polysachharides |
JP5693849B2 (ja) * | 2007-12-17 | 2015-04-01 | 株式会社明治 | 微生物複合体 |
MX2011001174A (es) * | 2008-07-29 | 2011-04-05 | Dsm Ip Assets Bv | Metodo para modificar material de carbohidrato sin almidon utilizando enzimas de peroxidasa. |
EA020808B1 (ru) * | 2008-08-11 | 2015-01-30 | ДСМ АйПи АССЕТС Б.В. | Деградация лигноцеллюлозного материала |
WO2010029312A1 (fr) * | 2008-09-12 | 2010-03-18 | University Court Of The University Of St. Andrews | Molécules fixant les glucides |
WO2010065908A2 (fr) * | 2008-12-05 | 2010-06-10 | Catchmark Jeffrey M | Compositions de biomolécule dégradables |
WO2010102297A2 (fr) | 2009-03-06 | 2010-09-10 | Parker Anthony A | Mousses contenant des protéines, préparation et utilisation de celles-ci |
CN105585996B (zh) * | 2009-03-06 | 2019-04-05 | 爱福特立 | 含有蛋白的乳液和粘合剂以及它们的制造与用途 |
FR2948132B1 (fr) * | 2009-07-20 | 2011-08-26 | Inst Francais Textile & Habillement | Association de produits cationiques et anioniques naturels comme liant pour support textile |
US20110086236A1 (en) * | 2009-10-13 | 2011-04-14 | The Penn State Research Foundation | Composites containing polypeptides attached to polysaccharides and molecules |
CN101736583B (zh) * | 2009-12-18 | 2012-07-25 | 东华大学 | 生物酶法改性甲壳素浆料的制备方法 |
PL2576661T3 (pl) * | 2010-06-07 | 2017-07-31 | Evertree | Substancje adhezyjne zawierające białka i ich wytwarzanie i zastosowanie |
CN102310451A (zh) * | 2010-07-06 | 2012-01-11 | 郭明辉 | 基于漆酶介体体系活化制备木质纤维板 |
CN103270052B (zh) * | 2010-11-23 | 2017-09-19 | 艾克海丽克斯有限责任公司 | 一种增加包含芳族部分的木质甘露聚糖和木聚糖的分子量的方法 |
US9109047B2 (en) * | 2011-02-03 | 2015-08-18 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd | High molecular ordered fibrilar structures, method for their preparation and uses thereof |
EP2751132B1 (fr) | 2011-09-01 | 2022-11-30 | Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. | Biopolymères adhésifs et leurs utilisations |
LT2753633T (lt) * | 2011-09-09 | 2017-04-10 | Evertree | Baltymo turintys klijai, jų gamyba ir panaudojimas |
RU2621798C2 (ru) | 2011-09-09 | 2017-06-07 | Эвертри | Белоксодержащие адгезивы и их получение и применение |
EP3666845A1 (fr) | 2012-07-30 | 2020-06-17 | Evertree | Adhésifs de protéine contenant un anhydride, de l'acide carboxylique et/ou un composé de sel de carboxylate et leur utilisation |
CN105026512B (zh) * | 2013-01-24 | 2017-09-22 | 佐治亚-太平洋化工品有限公司 | 包含疏水剂和稳定剂的组合物以及其制造和使用方法 |
WO2014201325A2 (fr) * | 2013-06-14 | 2014-12-18 | Yale University | Systèmes et procédés de dépolymérisation d'un biopolymère |
EP3024887B1 (fr) | 2013-07-26 | 2023-11-22 | The Penn State Research Foundation | Procédé pour préparation des compositions et revêtements polymères |
CN110776571B (zh) * | 2019-11-04 | 2021-08-10 | 福建省中医药研究院(福建省青草药开发服务中心) | 一种金属硫蛋白融合蛋白构建、固定化载体快速制备及其在重金属离子去除中的应用 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3037992C2 (de) * | 1980-10-08 | 1983-04-21 | Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig | Verfahren zur Herstellung eines Bindemittels für Holzwerkstoffe |
US4340673A (en) * | 1980-11-07 | 1982-07-20 | Merck & Co., Inc. | Process of producing modified glucans as anti-caries agent |
US5340731A (en) * | 1988-07-08 | 1994-08-23 | University Of British Columbia | Method of preparing a B-1,4 glycan matrix containing a bound fusion protein |
US5137819A (en) * | 1988-07-08 | 1992-08-11 | University Of British Columbia | Cellulose binding fusion proteins for immobilization and purification of polypeptides |
WO1994007998A1 (fr) * | 1992-10-06 | 1994-04-14 | Novo Nordisk A/S | Variantes de cellulase |
US5496934A (en) * | 1993-04-14 | 1996-03-05 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Nucleic acids encoding a cellulose binding domain |
CN1134726A (zh) * | 1993-10-04 | 1996-10-30 | 诺沃挪第克公司 | 一种包含修饰酶的酶制剂 |
JP3810794B2 (ja) * | 1994-07-26 | 2006-08-16 | ノボザイムス アクティーゼルスカブ | リグノセルロース基剤製品の製造方法および該方法によって得ることのできる製品 |
AU7560996A (en) * | 1995-11-08 | 1997-05-29 | Novo Nordisk A/S | Process for preparing a lignocellulose-based product, and product obtainable by the process |
US5874308A (en) * | 1996-01-16 | 1999-02-23 | University Of British Columbia | Compositions and methods for modulating cell proliferation using growth factor-polysaccharide binding fusion proteins |
AR005601A1 (es) * | 1996-01-29 | 1999-06-23 | Novozymes As | Proceso para la remocion o blanqueo de suciedad o manchas presentes en telas celulosicas y proceso para el lavado de telas celulosicas manchadas o sucias y composicion detergente |
EP0877799A1 (fr) * | 1996-01-29 | 1998-11-18 | Novo Nordisk A/S | Procede de desencollage d'un tissu contenant de la cellulose |
US5763385A (en) * | 1996-05-14 | 1998-06-09 | Genencor International, Inc. | Modified α-amylases having altered calcium binding properties |
EP0975745B1 (fr) * | 1996-10-28 | 2008-02-20 | Novozymes A/S | Expression extracellulaire de domaines de fixation de cellulose (cbd) au moyen de bacillus |
ES2270589T3 (es) * | 1998-04-03 | 2007-04-01 | Novozymes A/S | Tratamiento de tejido denim con una enzima pectolitica. |
JP2003533173A (ja) * | 1999-11-08 | 2003-11-11 | シービーデイ テクノロジーズ リミテッド | リグノセルロース系製品を調製するための方法および組成物ならびに該方法によって得られる製品 |
-
2000
- 2000-10-19 JP JP2001536815A patent/JP2003533173A/ja active Pending
- 2000-10-19 CA CA002390639A patent/CA2390639C/fr not_active Expired - Fee Related
- 2000-10-19 WO PCT/IL2000/000665 patent/WO2001034902A2/fr active Application Filing
- 2000-10-19 AU AU79431/00A patent/AU776069B2/en not_active Ceased
- 2000-10-19 BR BR0015519-5A patent/BR0015519A/pt not_active IP Right Cessation
- 2000-11-02 JP JP2001536097A patent/JP2004504803A/ja active Pending
- 2000-11-02 WO PCT/IL2000/000708 patent/WO2001034091A2/fr not_active Application Discontinuation
- 2000-11-02 BR BR0015520-9A patent/BR0015520A/pt not_active IP Right Cessation
- 2000-11-02 AU AU11729/01A patent/AU769461B2/en not_active Ceased
- 2000-11-02 CA CA002390568A patent/CA2390568A1/fr not_active Abandoned
- 2000-11-02 EP EP00973191A patent/EP1230374A4/fr not_active Ceased
- 2000-11-02 CN CNB008181403A patent/CN1329516C/zh not_active Expired - Fee Related
-
2002
- 2002-06-06 ZA ZA2002/04537A patent/ZA200204537B/en unknown
-
2004
- 2004-04-22 AU AU2004201766A patent/AU2004201766B8/en not_active Ceased
-
2005
- 2005-07-29 US US11/191,964 patent/US20050257905A1/en not_active Abandoned
-
2010
- 2010-09-20 US US12/885,611 patent/US20110005697A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2390639A1 (fr) | 2001-05-17 |
WO2001034902A2 (fr) | 2001-05-17 |
AU2004201766A1 (en) | 2004-05-20 |
CN1433473A (zh) | 2003-07-30 |
ZA200204537B (en) | 2005-07-27 |
US20110005697A1 (en) | 2011-01-13 |
EP1230374A2 (fr) | 2002-08-14 |
AU2004201766B8 (en) | 2005-09-08 |
CN1329516C (zh) | 2007-08-01 |
AU1172901A (en) | 2001-06-06 |
EP1230374A4 (fr) | 2004-10-13 |
CA2390568A1 (fr) | 2001-05-17 |
AU776069B2 (en) | 2004-08-26 |
WO2001034902A3 (fr) | 2003-01-03 |
US20050257905A1 (en) | 2005-11-24 |
WO2001034091A3 (fr) | 2002-01-17 |
BR0015519A (pt) | 2004-06-15 |
JP2004504803A (ja) | 2004-02-19 |
WO2001034091A2 (fr) | 2001-05-17 |
AU769461B2 (en) | 2004-01-29 |
AU7943100A (en) | 2001-06-06 |
BR0015520A (pt) | 2004-07-20 |
AU2004201766B2 (en) | 2005-08-18 |
JP2003533173A (ja) | 2003-11-11 |
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