CA2386193A1 - System and method for detecting and identifying molecular events in a test sample - Google Patents

System and method for detecting and identifying molecular events in a test sample Download PDF

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Publication number
CA2386193A1
CA2386193A1 CA002386193A CA2386193A CA2386193A1 CA 2386193 A1 CA2386193 A1 CA 2386193A1 CA 002386193 A CA002386193 A CA 002386193A CA 2386193 A CA2386193 A CA 2386193A CA 2386193 A1 CA2386193 A1 CA 2386193A1
Authority
CA
Canada
Prior art keywords
signal
test
fluid
molecular
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002386193A
Other languages
English (en)
French (fr)
Inventor
Robert G. Chapman
John J. Hefti
Barrett J. Bartell
Mark A. Rhodes
Min Zhao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DH Technologies Development Pte Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2386193A1 publication Critical patent/CA2386193A1/en
Abandoned legal-status Critical Current

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0673Handling of plugs of fluid surrounded by immiscible fluid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0645Electrodes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N22/00Investigating or analysing materials by the use of microwaves or radio waves, i.e. electromagnetic waves with a wavelength of one millimetre or more

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA002386193A 1999-10-13 2000-10-13 System and method for detecting and identifying molecular events in a test sample Abandoned CA2386193A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15917599P 1999-10-13 1999-10-13
US60/159,175 1999-10-13
US19170200P 2000-03-23 2000-03-23
US60/191,702 2000-03-23
PCT/US2000/028491 WO2001027610A2 (en) 1999-10-13 2000-10-13 System and method for detecting and identifying molecular events in a test sample

Publications (1)

Publication Number Publication Date
CA2386193A1 true CA2386193A1 (en) 2001-04-19

Family

ID=26855720

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002386193A Abandoned CA2386193A1 (en) 1999-10-13 2000-10-13 System and method for detecting and identifying molecular events in a test sample

Country Status (10)

Country Link
EP (1) EP1224453A2 (enExample)
JP (1) JP2003535310A (enExample)
KR (1) KR20020071853A (enExample)
CN (1) CN1425133A (enExample)
AU (3) AU8023500A (enExample)
CA (1) CA2386193A1 (enExample)
IL (1) IL149001A0 (enExample)
MX (1) MXPA02003815A (enExample)
NO (1) NO20021687L (enExample)
WO (1) WO2001027610A2 (enExample)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586946B2 (en) * 2000-11-13 2003-07-01 Signature Bioscience, Inc. System and method for detecting and identifying molecular events in a test sample using a resonant test structure
WO2002066983A2 (en) * 2001-02-01 2002-08-29 Signature Bioscience, Inc. Bioassay device for detecting molecular events
US20020168659A1 (en) * 2001-02-12 2002-11-14 Signature Bioscience Inc. System and method for characterizing the permittivity of molecular events
GB0117715D0 (en) * 2001-07-19 2001-09-12 Mrbp Res Ltd Microwave biochemical analysis
SG169225A1 (en) 2001-07-25 2011-03-30 Univ Princeton Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
US9678038B2 (en) 2001-07-25 2017-06-13 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
US20030032000A1 (en) * 2001-08-13 2003-02-13 Signature Bioscience Inc. Method for analyzing cellular events
KR100537504B1 (ko) 2003-01-20 2005-12-19 삼성전자주식회사 생물학적 시료 내에서의 단백질 검출을 위한 자동화된 유동 시스템
GB0328054D0 (en) * 2003-12-04 2004-01-07 Council Cent Lab Res Councils Fluid probe
GB0605273D0 (en) * 2006-03-16 2006-04-26 Council Cent Lab Res Councils Fluid robe
US10746680B2 (en) * 2006-11-16 2020-08-18 General Electric Company Sensing system and method
AU2008232616B2 (en) 2007-03-28 2014-08-07 Bionano Genomics, Inc. Methods of macromolecular analysis using nanochannel arrays
GB0716202D0 (en) * 2007-08-11 2007-09-26 Microvisk Ltd Improved fluid probe
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
GB201219016D0 (en) * 2012-10-23 2012-12-05 Liverpool John Moores University Electromagnetic wave sensing
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9791453B2 (en) * 2012-12-26 2017-10-17 International Business Machines Corporation Methods for determining binding capability of target ligands with G protein-coupled receptors using translocation through nanochannels
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
WO2014150853A1 (en) * 2013-03-15 2014-09-25 Inanovate, Inc. Analyte measurement using longitudinal assay
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
US9303310B2 (en) 2013-10-15 2016-04-05 International Business Machines Corporation Nanofluidic sensor comprising spatially separated functional sensing components
US9255321B2 (en) 2013-10-15 2016-02-09 Globalfoundries Inc. Directed surface functionalization on selected surface areas of topographical features with nanometer resolution
US10024851B2 (en) 2013-10-15 2018-07-17 International Business Machines Corporation Use of disulfide bonds to form a reversible and reusable coating for nanofluidic devices
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US9921181B2 (en) 2014-06-26 2018-03-20 International Business Machines Corporation Detection of translocation events using graphene-based nanopore assemblies
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
TWI569017B (zh) * 2014-11-14 2017-02-01 Nippon Mektron Kk Coaxial probe holding mechanism and electrical characteristics check device
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
CN108602065B (zh) * 2015-12-23 2021-07-27 维京遗传学Fmba 用于低温吸管的rfid识别系统
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN108257052B (zh) * 2018-01-16 2022-04-22 中南大学 一种在线学生知识评估方法及其系统
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
CN109192250B (zh) * 2018-08-01 2021-12-07 华东理工大学 一种多相催化中克服表面物种快速迁移的加速模拟方法
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
CN115598464A (zh) * 2022-10-08 2023-01-13 金华乐馨发制品有限公司(Cn) 一种电缆末端高低压电路的检测和修复装置及其方法
CN118834748A (zh) * 2024-08-21 2024-10-25 山东大学 一种基于微波控温的实时无接触数字pcr检测系统及方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0519250A3 (en) * 1991-06-10 1993-06-02 Miles Inc. Microparticle-labeled binding assay analyzed by microwave spectroscopy
AU7552194A (en) * 1993-07-26 1995-02-20 Phase Dynamics, Inc. System and method for monitoring substances and reactions
SE9700828D0 (sv) * 1997-03-07 1997-03-07 Asea Brown Boveri Förfarande och anordning för att bestämma ett flertal kvalitetsparametrar vilka beskriver tillståndet hos ett organiskt material
JP2002502028A (ja) * 1998-02-02 2002-01-22 シグネチャー バイオサイエンス,インコーポレイティド 分子結合現象を検出するための方法及び装置

Also Published As

Publication number Publication date
AU8023500A (en) 2001-04-23
NO20021687D0 (no) 2002-04-10
AU2008203111A1 (en) 2008-08-07
IL149001A0 (en) 2002-11-10
EP1224453A2 (en) 2002-07-24
JP2003535310A (ja) 2003-11-25
CN1425133A (zh) 2003-06-18
WO2001027610A9 (en) 2002-08-01
MXPA02003815A (es) 2002-09-30
KR20020071853A (ko) 2002-09-13
NO20021687L (no) 2002-06-12
AU2005203756A1 (en) 2005-09-15
WO2001027610A3 (en) 2002-03-28
WO2001027610A2 (en) 2001-04-19

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EEER Examination request
FZDE Discontinued