CA2381316A1 - Homogentisate-dioxygenase - Google Patents

Homogentisate-dioxygenase Download PDF

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Publication number
CA2381316A1
CA2381316A1 CA002381316A CA2381316A CA2381316A1 CA 2381316 A1 CA2381316 A1 CA 2381316A1 CA 002381316 A CA002381316 A CA 002381316A CA 2381316 A CA2381316 A CA 2381316A CA 2381316 A1 CA2381316 A1 CA 2381316A1
Authority
CA
Canada
Prior art keywords
plant
hgd
hppd
sequence
plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002381316A
Other languages
English (en)
Inventor
Marcus Ebneth
Karin Herbers
Michael Geiger
Isolde Saalbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SunGene GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2381316A1 publication Critical patent/CA2381316A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

Abstract

La présente invention concerne des cassettes d'expression, qui, sous le contrôle génétique de séquences d'acide nucléique de régulation, comprennent: a) la séquence d'acide nucléique codant pour la 4- hydroxyphénylpyruvate-dioxygénase (HPPD) ou pour un de ses équivalents fonctionnels; et/ou b) au moins une séquence d'acide nucléique (anti-HGD) apte à l'inhibition de l'activité de l'homogentisate-dioxygénase (HGD). Cette invention concerne également des vecteurs convenant à la production de végétaux à haute teneur en tocophérol, les végétaux transgéniques ainsi obtenus, ainsi qu'un procédé de production de végétaux transgéniques à haute teneur en tocophérol.
CA002381316A 1999-08-11 2000-08-10 Homogentisate-dioxygenase Abandoned CA2381316A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19937957.2 1999-08-11
DE19937957A DE19937957A1 (de) 1999-08-11 1999-08-11 Homogentisat-Dioxygenase
PCT/EP2000/007807 WO2001012827A2 (fr) 1999-08-11 2000-08-10 Homogentisate-dioxygenase

Publications (1)

Publication Number Publication Date
CA2381316A1 true CA2381316A1 (fr) 2001-02-22

Family

ID=7917993

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002381316A Abandoned CA2381316A1 (fr) 1999-08-11 2000-08-10 Homogentisate-dioxygenase

Country Status (7)

Country Link
EP (1) EP1200598A2 (fr)
AR (1) AR029762A1 (fr)
AU (1) AU6440900A (fr)
BR (1) BR0013127A (fr)
CA (1) CA2381316A1 (fr)
DE (1) DE19937957A1 (fr)
WO (1) WO2001012827A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002541851A (ja) 1999-04-15 2002-12-10 カルジーン エルエルシー イソプレノイド合成に関与するタンパク質の核酸配列
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
AU2001290522B2 (en) 2000-08-07 2006-11-30 Monsanto Technology Llc Methyl-D-erythritol phosphate pathway genes
DE10046462A1 (de) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Verbesserte Verfahren zur Vitamin E Biosynthese
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
ES2318004T3 (es) 2001-05-09 2009-05-01 Monsanto Technology Llc Genes tyra y usos de los mismos.
WO2003016482A2 (fr) 2001-08-17 2003-02-27 Monsanto Technology Llc Genes de methyltransferase et leurs utilisations
DE60235252D1 (de) 2001-10-25 2010-03-18 Monsanto Technology Llc Aromatische methyltransferasen und ihre verwendung
BR0308740A (pt) 2002-03-19 2007-01-09 Monsanto Technology Llc ácidos nucléicos e polipeptìdeos de homogentisado prenil transferase ("hpt"), e empregos destes
AU2003268083A1 (en) 2002-08-05 2004-02-23 Monsanto Technology, Llc Tocopherol biosynthesis related genes and uses thereof
GB2595633A (en) * 2020-04-15 2021-12-08 Univ Liverpool Anti-infective agents

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1238008A (zh) * 1996-07-25 1999-12-08 美国氰胺公司 Hppd基因和抑制剂
DE19730066A1 (de) * 1997-07-14 1999-01-21 Basf Ag DNA-Sequenz codierend für eine Hydroxyphenylpyruvatdioxygenase und deren Überproduktion in Pflanzen
FR2778527A1 (fr) * 1998-05-18 1999-11-19 Rhone Poulenc Agrochimie Nouvelle methode de production de tocopherols dans les plantes et plantes obtenues
JP2002525034A (ja) * 1998-08-05 2002-08-13 サンジーン ゲーエムベーハー ウント コー．カーゲーアーアー １−デオキシ−ｄ−キシルロース−５−リン酸シンターゼをコードするｄｎａ配列および植物におけるその過剰産生

Also Published As

Publication number Publication date
WO2001012827A2 (fr) 2001-02-22
WO2001012827A3 (fr) 2001-08-23
DE19937957A1 (de) 2001-02-15
WO2001012827A9 (fr) 2002-05-10
AR029762A1 (es) 2003-07-16
BR0013127A (pt) 2002-04-23
AU6440900A (en) 2001-03-13
EP1200598A2 (fr) 2002-05-02

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