CA2372164A1 - Synthese chimique et utilisation de domaines de recepteurs de proteines membranaires solubles - Google Patents

Synthese chimique et utilisation de domaines de recepteurs de proteines membranaires solubles Download PDF

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Publication number
CA2372164A1
CA2372164A1 CA002372164A CA2372164A CA2372164A1 CA 2372164 A1 CA2372164 A1 CA 2372164A1 CA 002372164 A CA002372164 A CA 002372164A CA 2372164 A CA2372164 A CA 2372164A CA 2372164 A1 CA2372164 A1 CA 2372164A1
Authority
CA
Canada
Prior art keywords
receptor
extramembranous
domain
ligand
membrane protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002372164A
Other languages
English (en)
Inventor
Gerd G. Kochendoerfer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gryphon Therapeutics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2372164A1 publication Critical patent/CA2372164A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne la synthèse chimique de domaines de récepteurs extramembranaires de récepteurs de protéines membranaires, et des compositions et des méthodes les utilisant. Les domaines de récepteurs extramembranaires comprennent les domaines cytosoliques et extracellulaires de liaison de ligands solubles de récepteurs de protéines membranaires. On produit les domaines de récepteur extramembranaire de l'invention en ligaturant dans des conditions chemosélectives de ligature chimique des premier et second peptides d'un domaine de récepteur extramembranaire d'un récepteur de protéine membranaire, où les peptides présentent des groupes réactifs chemosélectifs non protégés pouvant former une liaison covalente. Le produit de ligature est exposé à un tampon de pliage comportant un réactif chaotropique et un solvant organique se rapprochant de l'interface eau-lipide d'une membrane cellulaire. L'exposition au tampon de pliage est suivie par l'isolation du tampon de produit de ligature se fixant à un ligand du récepteur de protéine membranaire. La partie de fixation de ligand du produit de ligature produite par cette méthode représente un domaine de récepteur extramembranaire plié. L'invention est illustrée par la synthèse chimique totale du domaine N-terminal du récepteur de peptide-1 semblable au glucagon, par la démonstration de sa capacité à se fixer au ligand de peptide et par la caractérisation de sa carte bisulfure.
CA002372164A 1999-03-11 2000-03-09 Synthese chimique et utilisation de domaines de recepteurs de proteines membranaires solubles Abandoned CA2372164A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12427299P 1999-03-11 1999-03-11
US60/124,272 1999-03-11
PCT/US2000/006297 WO2000053624A1 (fr) 1999-03-11 2000-03-09 Synthese chimique et utilisation de domaines de recepteurs de proteines membranaires solubles

Publications (1)

Publication Number Publication Date
CA2372164A1 true CA2372164A1 (fr) 2000-09-14

Family

ID=22413849

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002372164A Abandoned CA2372164A1 (fr) 1999-03-11 2000-03-09 Synthese chimique et utilisation de domaines de recepteurs de proteines membranaires solubles

Country Status (7)

Country Link
EP (1) EP1159289A1 (fr)
JP (1) JP2002539136A (fr)
CN (1) CN1350544A (fr)
AU (1) AU768118B2 (fr)
CA (1) CA2372164A1 (fr)
HK (1) HK1046534A1 (fr)
WO (1) WO2000053624A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007004060A3 (fr) * 2005-05-05 2007-04-26 Valorisation Hsj Soc En Comman Modulateurs du recepteur de la cytokine et leurs utilisations
US8618054B2 (en) 2004-05-05 2013-12-31 Valorisation-Rechereche Société en Commandite Interleukin-1 receptor antagonists, compositions, and methods of treatment

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482425B2 (en) 1999-08-26 2009-01-27 Amylin Pharmaceuticals, Inc. Compositions for lipid matrix-assisted chemical ligation
KR20020097241A (ko) 2000-05-04 2002-12-31 에이브이아이 바이오파마 인코포레이티드 스플라이스-영역 안티센스 조성물 및 방법
US7785834B2 (en) 2005-11-10 2010-08-31 Ercole Biotech, Inc. Soluble TNF receptors and their use in treatment of disease
CA2666981A1 (fr) * 2006-10-20 2008-05-02 Ercole Biotech, Inc. Recepteurs de tnf solubles et leur utilisation pour le traitement de maladies
US8637452B2 (en) * 2011-02-23 2014-01-28 Massachusetts Institute Of Technology Water soluble membrane proteins and methods for the preparation and use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003321A1 (fr) * 1993-07-20 1995-02-02 Bionebraska, Inc. Procede d'endomodification de proteins
ATE354584T1 (de) * 1997-06-13 2007-03-15 Gryphon Therapeutics Inc Festphasige native chemische ligation von ungeschützten oder n-cystein-geschützten peptiden in wässrigen lösungen

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8618054B2 (en) 2004-05-05 2013-12-31 Valorisation-Rechereche Société en Commandite Interleukin-1 receptor antagonists, compositions, and methods of treatment
WO2007004060A3 (fr) * 2005-05-05 2007-04-26 Valorisation Hsj Soc En Comman Modulateurs du recepteur de la cytokine et leurs utilisations

Also Published As

Publication number Publication date
AU3874800A (en) 2000-09-28
JP2002539136A (ja) 2002-11-19
EP1159289A1 (fr) 2001-12-05
HK1046534A1 (zh) 2003-01-17
WO2000053624A1 (fr) 2000-09-14
CN1350544A (zh) 2002-05-22
AU768118B2 (en) 2003-12-04

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Legal Events

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EEER Examination request
FZDE Dead