CA2331777A1 - Expression de proteines eucaryotes fonctionnelles - Google Patents
Expression de proteines eucaryotes fonctionnelles Download PDFInfo
- Publication number
- CA2331777A1 CA2331777A1 CA002331777A CA2331777A CA2331777A1 CA 2331777 A1 CA2331777 A1 CA 2331777A1 CA 002331777 A CA002331777 A CA 002331777A CA 2331777 A CA2331777 A CA 2331777A CA 2331777 A1 CA2331777 A1 CA 2331777A1
- Authority
- CA
- Canada
- Prior art keywords
- expression
- polypeptide
- host cells
- hrp
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/80—Cytochromes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90245—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
L'invention concerne l'expression améliorée de séquences polynucléotidiques et polypeptidiques développées, lesquelles codent pour des enzymes eucaryotes, notamment des peroxydases, dans des systèmes d'expression classiques ou faciles. Pour obtenir les séquences améliorées, on peut utiliser différentes méthodes pour le développement dirigé de séquences polynucléotidiques. Les caractéristiques améliorées des polypeptides ou protéines ainsi générées sont les suivantes: meilleur repliement, sans formation de corps d'inclusion, et activité fonctionnelle retenue. Dans un mode de réalisation particulier, l'invention concerne l'expression améliorée du gène de la peroxydase du raifort et des enzymes de la peroxydase du raifort.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9440398P | 1998-07-28 | 1998-07-28 | |
US60/094,403 | 1998-07-28 | ||
US24723299A | 1999-02-09 | 1999-02-09 | |
US09/247,232 | 1999-02-09 | ||
PCT/US1999/017127 WO2000006718A2 (fr) | 1998-07-28 | 1999-07-28 | Expression de proteines eucaryotes fonctionnelles |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2331777A1 true CA2331777A1 (fr) | 2000-02-10 |
Family
ID=26788829
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002331777A Abandoned CA2331777A1 (fr) | 1998-07-28 | 1999-07-28 | Expression de proteines eucaryotes fonctionnelles |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1100891A2 (fr) |
JP (1) | JP2003503005A (fr) |
KR (1) | KR20010083086A (fr) |
AU (1) | AU5134599A (fr) |
CA (1) | CA2331777A1 (fr) |
IL (1) | IL140509A0 (fr) |
MX (1) | MXPA01000224A (fr) |
WO (1) | WO2000006718A2 (fr) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
WO1999021979A1 (fr) | 1997-10-28 | 1999-05-06 | Maxygen, Inc. | Vecteurs du papillomavirus humain |
EP1030861A4 (fr) | 1997-10-31 | 2001-09-05 | Maxygen Inc | Modification du tropisme viral et de la diversite d'especes hote par recombinaison du genome viral |
US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
US6337186B1 (en) | 1998-06-17 | 2002-01-08 | Maxygen, Inc. | Method for producing polynucleotides with desired properties |
WO2001072999A1 (fr) * | 2000-03-27 | 2001-10-04 | California Institute Of Technology | Expression de proteines eucaryotes fonctionnelles |
FR2810339B1 (fr) * | 2000-06-14 | 2004-12-10 | Hoechst Marion Roussel Inc | Banques combinatoires ameliorees par recombinaison dans la levure et procede d'analyse |
JP2002199890A (ja) * | 2000-10-23 | 2002-07-16 | Inst Of Physical & Chemical Res | 生分解性ポリエステル合成酵素の改変方法 |
EP1470219A4 (fr) | 2001-04-16 | 2005-10-05 | California Inst Of Techn | Variantes d'oxygenase a cytochrome p450 mues par le peroxyde |
WO2003008563A2 (fr) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Cytochrome p450 oxygenases ameliorees |
US7524664B2 (en) | 2003-06-17 | 2009-04-28 | California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
DK1660646T3 (en) | 2003-08-11 | 2015-03-09 | California Inst Of Techn | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8026085B2 (en) | 2006-08-04 | 2011-09-27 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
US8802401B2 (en) | 2007-06-18 | 2014-08-12 | The California Institute Of Technology | Methods and compositions for preparation of selectively protected carbohydrates |
US9322007B2 (en) | 2011-07-22 | 2016-04-26 | The California Institute Of Technology | Stable fungal Cel6 enzyme variants |
EP2992021B1 (fr) * | 2013-04-30 | 2020-07-22 | Intas Pharmaceuticals Limited | Nouveau procédé de clonage, d'expression et de purification pour la préparation de ranibizumab |
EP3724212A4 (fr) * | 2018-01-19 | 2021-11-24 | OBI Pharma, Inc. | Expression de protéine crm197 |
CN112852800B (zh) * | 2020-01-09 | 2023-02-03 | 中国科学院天津工业生物技术研究所 | 一种信号肽突变体及其应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8723662D0 (en) * | 1987-10-08 | 1987-11-11 | British Bio Technology | Synthetic gene |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
FR2752366B1 (fr) * | 1996-08-14 | 1998-12-11 | Aguilar Elisabeth | Tablier utilise en particulier pour les travaux de cuisine |
-
1999
- 1999-07-28 AU AU51345/99A patent/AU5134599A/en not_active Abandoned
- 1999-07-28 IL IL14050999A patent/IL140509A0/xx unknown
- 1999-07-28 KR KR1020017000036A patent/KR20010083086A/ko not_active Application Discontinuation
- 1999-07-28 CA CA002331777A patent/CA2331777A1/fr not_active Abandoned
- 1999-07-28 EP EP99935983A patent/EP1100891A2/fr not_active Withdrawn
- 1999-07-28 MX MXPA01000224A patent/MXPA01000224A/es unknown
- 1999-07-28 JP JP2000562500A patent/JP2003503005A/ja not_active Withdrawn
- 1999-07-28 WO PCT/US1999/017127 patent/WO2000006718A2/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO2000006718A2 (fr) | 2000-02-10 |
WO2000006718A3 (fr) | 2000-06-15 |
MXPA01000224A (es) | 2002-10-17 |
KR20010083086A (ko) | 2001-08-31 |
AU5134599A (en) | 2000-02-21 |
EP1100891A2 (fr) | 2001-05-23 |
JP2003503005A (ja) | 2003-01-28 |
IL140509A0 (en) | 2002-02-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |