CA2323060A1 - Detection of neoplasia by analysis of saliva - Google Patents
Detection of neoplasia by analysis of saliva Download PDFInfo
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- CA2323060A1 CA2323060A1 CA002323060A CA2323060A CA2323060A1 CA 2323060 A1 CA2323060 A1 CA 2323060A1 CA 002323060 A CA002323060 A CA 002323060A CA 2323060 A CA2323060 A CA 2323060A CA 2323060 A1 CA2323060 A1 CA 2323060A1
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Classifications
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/82—Translation products from oncogenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
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- Wood Science & Technology (AREA)
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- Medicinal Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/038,637 | 1998-03-10 | ||
| US09/038,637 US6235470B1 (en) | 1993-11-12 | 1998-03-10 | Detection of neoplasia by analysis of saliva |
| PCT/US1999/005220 WO1999046408A1 (en) | 1998-03-10 | 1999-03-10 | Detection of neoplasia by analysis of saliva |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2323060A1 true CA2323060A1 (en) | 1999-09-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002323060A Abandoned CA2323060A1 (en) | 1998-03-10 | 1999-03-10 | Detection of neoplasia by analysis of saliva |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US6235470B1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1062366B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP2002505888A (cg-RX-API-DMAC7.html) |
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| AU (1) | AU769035B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2323060A1 (cg-RX-API-DMAC7.html) |
| DE (1) | DE69940969D1 (cg-RX-API-DMAC7.html) |
| NO (1) | NO20004504L (cg-RX-API-DMAC7.html) |
| WO (1) | WO1999046408A1 (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114885938A (zh) * | 2022-02-25 | 2022-08-12 | 厦门迈威生物科技有限公司 | 一种浆膜腔积液脱落细胞保存液及其生产方法 |
Families Citing this family (36)
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| US6235470B1 (en) * | 1993-11-12 | 2001-05-22 | The Johns Hopkins University School Of Medicine | Detection of neoplasia by analysis of saliva |
| EP0956365A4 (en) * | 1996-08-28 | 2004-08-25 | Univ Johns Hopkins Med | METHOD FOR DETECTING CELL PROLIFERATIVE DEREGULATIONS |
| US7888324B2 (en) * | 2001-08-01 | 2011-02-15 | Genzyme Corporation | Antisense modulation of apolipoprotein B expression |
| US7407943B2 (en) * | 2001-08-01 | 2008-08-05 | Isis Pharmaceuticals, Inc. | Antisense modulation of apolipoprotein B expression |
| AU2002347835A1 (en) * | 2001-12-14 | 2003-07-24 | Zila Biotechnology, Inc. | Light-directed molecular analysis for cancer prognosis and diagnosis |
| US20060009410A1 (en) * | 2002-11-13 | 2006-01-12 | Crooke Rosanne M | Effects of apolipoprotein B inhibition on gene expression profiles in animals |
| US7511131B2 (en) | 2002-11-13 | 2009-03-31 | Genzyme Corporation | Antisense modulation of apolipoprotein B expression |
| CA2513747C (en) | 2003-01-24 | 2017-03-07 | University Of Utah | Methods of predicting mortality risk by determining telomere length |
| WO2005089414A2 (en) * | 2004-03-17 | 2005-09-29 | The Johns Hopkins University | Neoplasia diagnostic compositions and methods of use |
| US20050287558A1 (en) | 2004-05-05 | 2005-12-29 | Crooke Rosanne M | SNPs of apolipoprotein B and modulation of their expression |
| PT1786472E (pt) * | 2004-08-10 | 2013-03-06 | Genzyme Corp | Modulação anti-sentido de expressão de apolipoproteína |
| EP2015758B1 (en) | 2006-05-05 | 2014-04-02 | Isis Pharmaceuticals, Inc. | Compounds and methods for modulating expression apob |
| AU2007249805B2 (en) * | 2006-05-12 | 2013-01-10 | University Of Miami | Biomarkers for detection and diagnosis of head and neck squamous cell carcinoma |
| CA2682082A1 (en) * | 2007-03-24 | 2008-10-02 | Genzyme Corporation | Administering antisense oligonucleotides complementary to human apolipoprotein b |
| US20090023138A1 (en) * | 2007-07-17 | 2009-01-22 | Zila Biotechnology, Inc. | Oral cancer markers and their detection |
| US8012678B2 (en) * | 2007-07-24 | 2011-09-06 | Wisconsin Alumni Research Foundation | Biomarkers for human papilloma virus-associated cancer |
| AU2009329987B2 (en) | 2008-12-22 | 2016-08-04 | University Of Utah Research Foundation | Monochrome multiplex quantitative PCR |
| US9107933B2 (en) | 2009-03-16 | 2015-08-18 | Isis Pharmaceuticals, Inc. | Compositions and methods of targeting apolipoprotein B for the reduction of apolipoprotein C-III |
| ITBO20090554A1 (it) * | 2009-08-27 | 2011-02-28 | Maria Gabriella Fornaro | Metodo per individuare la predisposizione al carcinoma della prostata. |
| AU2011230633B2 (en) * | 2010-03-26 | 2015-01-22 | Mayo Foundation For Medical Education And Research | Methods and materials for detecting colorectal neoplasm |
| WO2013074793A1 (en) | 2011-11-15 | 2013-05-23 | University Of Miami | Methods for detecting human papillomavirus and providing prognosis for head and neck squamous cell carcinoma |
| US20140370505A1 (en) * | 2011-12-31 | 2014-12-18 | Telome Health, Inc. | Saliva-derived measures of telomere abundance and sample collection device |
| US9506116B2 (en) | 2013-03-14 | 2016-11-29 | Mayo Foundation For Medical Education And Research | Detecting neoplasm |
| JP6546158B2 (ja) | 2013-05-22 | 2019-07-17 | テロメア ダイアグノスティクス インコーポレイテッド | 短いテロメアの存在量の測定 |
| WO2015153284A1 (en) | 2014-03-31 | 2015-10-08 | Mayo Foundation For Medical Education And Research | Detecting colorectal neoplasm |
| US10184154B2 (en) | 2014-09-26 | 2019-01-22 | Mayo Foundation For Medical Education And Research | Detecting cholangiocarcinoma |
| CN107208145A (zh) | 2014-12-30 | 2017-09-26 | 端粒诊断公司 | 多重定量pcr |
| US10030272B2 (en) | 2015-02-27 | 2018-07-24 | Mayo Foundation For Medical Education And Research | Detecting gastrointestinal neoplasms |
| EP3274440A4 (en) | 2015-03-27 | 2019-03-06 | Exact Sciences Corporation | Detecting esophageal disorders |
| KR20250172724A (ko) | 2015-08-31 | 2025-12-09 | 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 | 위 신생물 검출 방법 |
| AU2017250663B2 (en) | 2016-04-14 | 2023-06-15 | Mayo Foundation For Medical Education And Research | Detecting pancreatic high-grade dysplasia |
| US10370726B2 (en) | 2016-04-14 | 2019-08-06 | Mayo Foundation For Medical Education And Research | Detecting colorectal neoplasia |
| CA3054836A1 (en) | 2017-02-28 | 2018-09-07 | Mayo Foundation For Medical Education And Research | Detecting prostate cancer |
| CA3084584A1 (en) | 2017-11-30 | 2019-06-06 | Mayo Foundation For Medical Education And Research | Detecting breast cancer |
| JP7534962B2 (ja) | 2018-06-19 | 2024-08-15 | ビオンテック ユーエス インコーポレイテッド | ネオ抗原およびその使用 |
| USD1034632S1 (en) * | 2022-05-03 | 2024-07-09 | SimpliSafe, Inc. | Display screen with graphical user interface |
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| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5527676A (en) | 1989-03-29 | 1996-06-18 | The Johns Hopkins University | Detection of loss of the wild-type P53 gene and kits therefor |
| DE69032864T3 (de) | 1989-03-29 | 2013-01-31 | The Johns Hopkins University | Nachweis des Ausfalls des Wildtyps des p53-Gens |
| EP1013666B1 (en) | 1990-06-27 | 2006-05-10 | Princeton University | Protein complex p53/p90 |
| AU662906B2 (en) | 1991-06-26 | 1995-09-21 | F. Hoffmann-La Roche Ag | Methods for detection of carcinoma metastases by nucleic acid amplification |
| ATE253645T1 (de) | 1992-04-01 | 2003-11-15 | Univ Johns Hopkins Med | Verfahren zur bestimmung von säugetier nukleinsäuren aus stuhlproben und dafür benötigte reagenzien |
| WO1993022456A1 (en) | 1992-04-27 | 1993-11-11 | Trustees Of Dartmouth College | Detection of gene sequences in biological fluids |
| US5561041A (en) | 1993-11-12 | 1996-10-01 | The Johns Hopkins University School Of Medicine | Nucleic acid mutation detection by analysis of sputum |
| US6235470B1 (en) | 1993-11-12 | 2001-05-22 | The Johns Hopkins University School Of Medicine | Detection of neoplasia by analysis of saliva |
| CH686982A5 (fr) * | 1993-12-16 | 1996-08-15 | Maurice Stroun | Méthode pour le diagnostic de cancers. |
| US5589579A (en) | 1994-07-19 | 1996-12-31 | Cytoclonal Pharmaceutics, Inc. | Gene sequence and probe for a marker of non-small cell lung carinoma |
| MXPA00009654A (es) * | 1994-08-31 | 2005-09-08 | Univ Johns Hopkins Med | Deteccion de secuencias de acidos nucleicos hipermutables en tejidos. |
| EP0956365A4 (en) * | 1996-08-28 | 2004-08-25 | Univ Johns Hopkins Med | METHOD FOR DETECTING CELL PROLIFERATIVE DEREGULATIONS |
| DE19712332A1 (de) * | 1997-03-25 | 1998-10-01 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis von Mikrosatelliten-Instabilität zur Tumordiagnostik |
| US6309827B1 (en) * | 1997-03-28 | 2001-10-30 | Orasure Technologies, Inc. | Simultaneous collection of DNA and non-nucleic analytes |
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1999
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- 1999-03-10 DE DE69940969T patent/DE69940969D1/de not_active Expired - Lifetime
- 1999-03-10 JP JP2000535774A patent/JP2002505888A/ja active Pending
- 1999-03-10 EP EP99911289A patent/EP1062366B1/en not_active Expired - Lifetime
- 1999-03-10 AT AT99911289T patent/ATE433498T1/de not_active IP Right Cessation
- 1999-03-10 WO PCT/US1999/005220 patent/WO1999046408A1/en not_active Ceased
- 1999-03-10 CA CA002323060A patent/CA2323060A1/en not_active Abandoned
-
2000
- 2000-09-08 NO NO20004504A patent/NO20004504L/no unknown
-
2001
- 2001-05-22 US US09/863,806 patent/US20020197608A1/en not_active Abandoned
-
2004
- 2004-01-05 JP JP2004000679A patent/JP2004159661A/ja active Pending
- 2004-01-09 US US10/754,478 patent/US7279312B2/en not_active Expired - Fee Related
-
2007
- 2007-10-08 US US11/868,683 patent/US7601521B2/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114885938A (zh) * | 2022-02-25 | 2022-08-12 | 厦门迈威生物科技有限公司 | 一种浆膜腔积液脱落细胞保存液及其生产方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU769035B2 (en) | 2004-01-15 |
| AU2996999A (en) | 1999-09-27 |
| NO20004504D0 (no) | 2000-09-08 |
| HK1033765A1 (en) | 2001-09-21 |
| DE69940969D1 (de) | 2009-07-23 |
| EP1062366A4 (en) | 2002-05-08 |
| ATE433498T1 (de) | 2009-06-15 |
| US20050009040A1 (en) | 2005-01-13 |
| US6235470B1 (en) | 2001-05-22 |
| US7601521B2 (en) | 2009-10-13 |
| US20020197608A1 (en) | 2002-12-26 |
| US7279312B2 (en) | 2007-10-09 |
| WO1999046408A1 (en) | 1999-09-16 |
| JP2004159661A (ja) | 2004-06-10 |
| EP1062366B1 (en) | 2009-06-10 |
| NO20004504L (no) | 2000-11-08 |
| EP1062366A1 (en) | 2000-12-27 |
| JP2002505888A (ja) | 2002-02-26 |
| AU2003266475A1 (en) | 2004-01-22 |
| US20080124729A1 (en) | 2008-05-29 |
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| EEER | Examination request | ||
| FZDC | Discontinued application reinstated | ||
| FZDE | Discontinued |
Effective date: 20121217 |