CA2305507A1

CA2305507A1 - A new powerful cosmetic or pharmaceutical composition

Info

Publication number: CA2305507A1
Application number: CA002305507A
Authority: CA
Inventors: Deguang Zhu
Original assignee: Individual
Current assignee: Individual
Priority date: 1997-10-16
Filing date: 1998-10-15
Publication date: 1999-04-22
Also published as: JP2002509855A; EP1051142A4; CN1275897A; KR20010031170A; WO1999018920A1; EP1051142A1

Abstract

The present invention relates to an extract of animal prancreas for topical application to the skin or wound which is a new, powerful, multi-function, cosmetic or pharmaceutical composition. It has a function of moisturizing skin, a function of making skin a comfortable silky smooth feel, a function of accelerating wound healing, a function of skin cleansing, a function of peeling stratum corneum, a function of treating keratoses, a function of improving tissue cell metabolism, a function of promoting cell turnover, and a function of enhancing the quality of skin or scalps cells, etc. It can be formulated with cosmetically or pharmaceutically acceptable ingredients into varied products to satisfy some cosmetic or pharmaceutical needs. The present invention also describes a method of preparing the extract of animal pancreas.

Description

WO 99/18920 PCT/US9$/21794 Patent Application of A New Powerful Cosmetic or Pharmaceutical Composition Background-Field of Invention The present invention relates to a crude extract (use extract for following) of animal pancreas, specifically to such extract which is used as a new, powerful, multi-function, cosmetic or pharmaceutical composition.
Background-Description of Prior Art Pancreas has both endocrine and exocrine functions, and therefore, contains many biological active substances such as insulin, glucagon, somatostatin, pancreatic polypeptide, kallikrein, and pancreatin, etc. Certain active substances of animal pancreas have recently been used as cosmetic ingredients or ingredients of accelerating wound healing. So far, it includes kallikrein, pancreatin, and insulin.
Kallikrein is used as a vasodilator. It is reported to improve cell mitosis, wound healing and oxygen uptake, and protect from UV radiation (Natalia Michalun et al.).
Pancreatin contains several enzymes that give it proteolytic, amylolytic and lipolytic activity.
Pancreatin is used as a raw material in biological exfoliant to help remove cells from corneum layer i CA 02305507 2000-04-03 .(~~~~t~ ~7~ ~'~'r.
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(Natalia Michalun et al.). . ~ , Insulin is used as an ingredient of accelerating wound healing in Lindenbaum's formulations (CJ.S. Pat. No. 5461030 and 5591709).
But the effects of other substances of pancreas on skin and wound have not been known well.
There is a possibility that they also are valuable substances in cosmetic and wound healing field.
The extract of animal pancreas is a mixture; it contains many biologic active substances and other substances. Its effect on skin and wound has also not been understood yet.
Perhaps, it is due to that pancreas contains many digestive enzymes such as trypsin, chymotrypsin, elastase, lipase, and phospholipase (Chandrasoma, Underwood, and Isselbacher). These enzymes are also called as pancreatin. They digest cruelly normal tissue cells and result in their necrosis and bleeding. For example, trypsin and chymotrypsin can cause necrosis and hemorrhage, elastase causes vascular injury and local hemorrhage, lipase and phospholipase cause respectively local fat necrosis, and cell membrane injury (Chandrasoma, and Isselbacher). Although some hormones, such as insulin and kallikrein, are beneficial to wound healing, their amounts in the pancres are far less than that of digestive enzymes, because the most of pancreas tissue are to produce pancreatin (Underwood).
Therefore, the anticipated effect of the extract on skin and wound should be harmful or can even be considered dangerous, even though the extract also contains insulin and kallikrein, because the damages of digestive enzymes to pancreas and peripancreatic tissues in pancreatitis is under this condition with insulin and kallikrein too (Chandrasoma, Underwood, and Isselbacher).
Objects and Advantages However, present invention has prepared an extract of animal pancreas, and topically applied it to human skin and wound. Surprising is that this extract does not damage any normal tissue cells and wound. Instead, it helps wound healing, improves tissue cell metabolism, promotes cell turnover, enhances skin quality, moisturizes skin, cleanses skin and makes skin to have a silky smcjoth feeling. Although the extract peels part of stratum corneum, this effect is self limited, and it does not digest all stratum corneum. In addition, it can also be used to treat ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc. All above beneficial effects are not anticipated, but they meet the requirement used as a multi-function, cosmetic and pharmaceutical composition.
Page 2 of 13 CA 02305507 2000-04-03 'y tPEAfUS 11 MAY 1999 objects:
The objects of present invention is to provide a method of preparing tl:e extract of animal pancreas; to use said extract to make a product of cosmetic or pharmaceutical for treating skin, scalp, wound or some other diseases; and to disclose new active compositions in said extract.
Advantages:
1. Have a powerful function to accelerate wound healing, for example when it is topically applied to skin, chap and crack disappear in a few days. However, present invention is different from Lindenbaum's references (LJ.S. Patents No. 5461030 and 5591709) because his references did not comprise kallikrein, pancreatin and some other unknown compositions.

2. Have a powerful function to peel stratum corneum, but it only peels part of corneum, never peels all the stratum corneum, and never damages any normal skin cells, even though ,,.~...
concentrated or purified extract is used. This advantage will make that it can be safely used in cosmetic and pharmaceutical field to substitute hydroxy acids for decreasing the sign of aging.
Hydroxy acids have many side effects to skin. The peeling effect of present invention is probably attributed to pancreatin in the extract of pancreas. Michalun reference also recited that pancreatin had exfoliating effect on stratum corneum, but he did not disclose above peeling characteristic. Perhaps, it is the reason that pancreatin has not yet be used in home-using cosmetic and pharmaceutical products, because pancreatin has always been considered very harmful to tissue cells. In addition, its peeling function also is distinctive than Rawling's reference (U.S. Patent No. 5554366). Rawlings active composition is stratum corneum chymotrypsin-like enzyme, but the extract of the present invention does not comprise stratum corneum chymotrypsin-like enzyme.

3. Can be used to treat ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc, but does not damage normal tissue cells.

4. Make skin to have a very comfortable silky smooth feel. This feel can last several days. In addition, it provides a naturally healthy look and feel without tacky feel or greasy look for skin.

5. Have a cleansing function without irritation to skin while it plays a role in moisturizing skin.
For example, use consiste~ly it for several days, then the dirt firmly stuck in dactylogram and Page 3 of 13 AMENDED SHEET

CA 02305507 2000-04-03 'r orTnl~ 9~~179~
A~EAIUS 1 ~ MAY ~g99 pore can t~e washed out easily.

6. Improve tissue cell metabolism, enhance skin, scalp, and wound quality, promote cell turnover and protect normal skin and scalp. For example, it regulates the renewal and differentiation of the keratinocytes, so as to have an anti-aging activity.

7. Be very safe. Although high concentration or purified extract is applied topically to skin, it does not damage normal skin.

8. Have a longer and more stable activity. Although the extract contains many enzymes and hormones, their activities are more stable and can last more than one year even at room temperature.
Suamnary Briefly, the present invention pertains to discovering a new, powerful, mufti-function cosmetic or pharmaceutical composition for topical application to the skin or wound, and to a method of making this composition.
Description of Invention All of the pancreases of mammalian animals and many solvents (organic and inorganic) can be used to prepare the extract of animal pancreas, but preferably, I use swine pancreas, alcohol, and water to do it. Preferably the concentration of alcohol is higher than 60%, more preferably use 95%
alcohol or much higher concentration.
Fresh or deepfrozen animal pancreases are ground or chopped up and suspended, while stirnng, in double volume of 95% alcohol. The mixture is extracted for about several hours to 24 hours, then the mixture is centrifuged for a short time at about 5,000 rpm. Collect supernatant, and do not contaminate.solid substance. Although the supernatant can topically be applied to the skin for skin care purposes after diluted by a dilutant, it is irritant to skin because it comprises too much alcohol.
In order to decrease irritation to the skin or wound of alcohol in the extract of animal pancreas, above supernatant is concentrated by using vacuum pump or simply by blowing air into the supernatant to evaporate alcohol, but is not dried because dried extract is difficult to be completely dissolved again. Preferably, tie supernatant is concentrated to about one-fourth of volume. For Page 4 of 13 AMENDED SHEET' example, 10 ml of the supernatant is concentrated to about 2.5 ml by evaporating alcohol. This concentrated supernatant is an extract of animal pancreas. It is a yellow liquid including a water phase and an oil phase; its pH value is between 6 and 7.
Example 50 g of swine pancreas is ground and suspended, while stirring, in 100 ml of 95% alcohol. The mixture is extracted at room temperature for about 12 hours, then is centrifuged for 10 minutes at 5,000 rpm. Transfer supernatant into a fresh container, and do not contaminate any solid substances. About 90 ml of the supernatant can be collected. Vacuum 90 ml of supernatant until the volume is decreased to about 22 ml. Now the extract of animal pancreas is gained.
Finally, the extract of animal pancreas is formulated with cosmetically or pharmaceutically acceptable ingredients into a variety of emulsions or solutions to satisfy some cosmetic or pharmaceutical needs. Preferably, the composition comprises about 1 to 13%
more preferably 1 to 3% by weight of the extract of animal pancreas.
Examples The Solution for Normal Skin % w/w Extract of animal pancreases2.0 Ceteareth-20 0.2 Methylparaben 0.2 Perfume qs Water to 100 Daytime Protective Emulsion Deionized water 76.9 Carbomer 0.2 Disodium EDTA 0.1 Methylparaben 0.2 Octyl methoxycinnamate 7.5 CA 02305507 2000-04-03 _ PCTIUs 9 8 2 I 7 9 ~ ,.
jPEAIUS ~ ~ ~'~AY )999 Cyclomethicone 5.0 Benzophenone-3 3.0 Stearic Acid 2.0 Phenotrimethicone 1.0 Tocopheryl acetate 1.0 Glyceryl Stearate 0.5 Cetyl alcohol 0.2 Propylparaben 0.1 Iron oxides 0.002 Sodium hydroxide (101 0.3 Extract of animal pancreas 2.0 Perfume qs The extract of pancreas can also directly and topically be applied to skin or scalp without dilution and without formulating with other cosmetic and pharmaceutical ingredients. It can even be used at concentrated condition without damage to skin and scalp. However, the alcohol in the extract is irritant to wound of skin and scalp. In addition, the water phase and oil phase of the extract can separately be used, or separately used as a cosmetic and pharmaceutical composition to be formulated with other ingredients into varied products of cosmetic or pharmaceutical. In order to have a more specific function, the extract can further be processed by adjusting pH, salting-out, dialyzing, crystallizing, chromatographing, or electrophoresis etc. to eliminate some unwanted compositions. The pancreatin separated by means of above methods can safely and self limited peel stratum corneum without damage to normal tissue cells. Further purified trypsin and chymotrypsin can also be used to peel stratum corneum. The pancreatin, trypsin and chymotrypsin can be used to treat ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc.
Page 6 of 13 AMENDED SHEET

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~'~FAIIIS 1 1 MAC' 199 The extract of animal pancreas comprises many active compositions such as insulin, glucagon, somatostatin, pancreatic polypeptide, kallikrein, pancreatin, fat, and some unknown active compositions. Some of them are mutually antagonistic and some cooperative in function. By means of the experiments, it has been proved that the extract of animal pancreas is very beneficial to skin, scalp and wound and it does not damage normal tissue cells. Therefore, it is a very good and very useful cosmetic and pharmaceutical composition. However, the mechanism of effect, especially combined effect of varied compositions in the extract, is di~cult to foretell, because the crude extract of animal pancreas includes many active composition, some of them have been known and some not. Some unknown compositions probably provide the important functions such as long-lasting moisturizing, long-lasting silky smooth feeling, skin cleansing, and accelerating wound healing function.
Page 7 of 13 AMENDED SHEEP

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Operation of Invention The composition according to the invention is intended primarily as the products for topical application to human skin or wound, for protecting normal skin from dry, restoring the skin to a healthy, hydrated, supple and soft condition, treating dry skin, alleviating dandruff, accelerating wound healing, making skin a comfortable silky smooth feel, cleansing skin, and enhancing the quality of skin and scalp cells, etc.
In use, a small quantity of the composition, for example from 0.5 to 5 ml, is applied to area of the skin, scalp, or wound, it is then spread over and/or rubbed into the skin, scalp, or wound using the hand, forgers or a suitable device.
Conclusion, Ramifications and Scope Accordingly, the reader will see that the extract of the present invention is an excellently multi-function cosmetic or pharmaceutical composition. It can be used to protect normal skin from dry, restore the skin to a healthy, hydrated, supple and soft condition, treat dry skin, alleviate dandruff, provide a very comfortable silky smooth feel for the skin, cleanse skin, accelerate wound healing, enhance the quality of skin and scalp cells, etc. It can also be used in combination with many cosmetically or pharmaceutically acceptable ingredients to provide much broader functions.
Furthermore, it has the additional advantages in that it provides the longest effect of moisturization on skin;
. it keeps skin in naturally health feel and look;
. it offers users a comfortable long-lasting silky smooth skin feel;
. it has an effect of cleansing on skin;
. it has an effect of exfoliating the cells from corneum layer;
Page 8 of 13 ~A~IENDED SH~Er p TI~
1 ~ MAIf 1999 . it has an excellent effect on enhancing wound healing; and . it has an effect of enhancing the quality of skin or scalp cells.
Although the description above contains many specificities, these should not be construed as limitations on the scope of the invention, but rather as an exemplification of one preferred embodiment thereof. Many other variations are possible. For example, its effect on wound healing can also be used for other wounds, such as burn wounds, for tissue or organ grafting, etc.; while is used to enhance wound healing, it also has the effect on wound debridement; it can also is formulated in about 70% alcohol to create a sterilizer, which sterilizes skin as well as protects skin from the side effect of alcohol on degrading the protective fatty acid mantle of skin; and etc.
Accordingly, the scope of the invention should be determined not by the embodiments illustrated, but by the appended claims and their legal equivalents.
Page 9 of 13 ~IIE~1DED SH~~

Claims

Claims: I claim:

1. A method of preparing a crude extract of animal pancreas, which comprises adding an organic solvent to pancreatic material to thereby extract the a hydosoluble compositions of said animal pancreas and a composition of said animal pancreas which is soluble in said organic solvent, whereby said crude extract will be used as a cosmetic or pharmaceutical composition.

2. The method of claim 1 wherein said organic solvent is alcohol.

3. A composition for topical application to skin, scalp, or wound which treats dry, chapped or cracked skin, protects normal skin, makes skin to have a silky smooth feel, accelerates wound healing, peels the cells from stratum corneum, treats ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc, cleanses skin, improves tissue cell metabolism, promotes cell turnover, enhances the quality of skin, scalp, or wound, or protects skin and scalp from aging comprising a crude extract of animal pancreas or at least one component of said crude extract.

4. The composition of claim 3 wherein said composition for topical application to skin or scalp which protects normal skin and treats dry, chapped or cracked skin comprises at least one component of said crude extract.

5. The composition of claim 3 wherein said composition for topical application to skin which makes skin to have a silky smooth feeling comprises at least one component of said crude extract.

6.The composition of claim 3 wherein said composition for topical application to wound which accelerates wound healing comprises at least one component of said crude extract.

7. The composition of claim 3 wherein said composition for topical application to skin, scalp, or wound which improves tissue cell metabolism, promotes cell turnover, or enhances the quality of skin, scalp, or wound comprises at least one component of said crude extract.

8. The composition of claim 3 wherein said composition for topical application to skin, scalp, or wound which cleanses skin, scalp, or wound comprises at least one component of said Page 10 of 13 crude extract.

9. The composition of claim 3 wherein said composition for topical application to skin or scalp which peels the cells of stratum corneum or treats ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc comprises at least one component of said crude extract.

10. The composition of claim 3 wherein said composition for topical application to skin or scalp which peels the cells of stratum corneum or treats ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc comprises trypsin or chymotrypsin of said crude extract.

11. A method of protecting normal skin, making skin to have a silky smooth feeling, treating dry, chapped or cracked skin, accelerating wound healing, peeling the cells of stratum corneum, treating ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc, cleansing skin, improving tissue cell metabolism, promoting cell turnover, enhancing the quality of skin, scalp or wound, or protecting skin and scalp from aging comprising a crude extract of animal pancreas or at least one component of said extract.

12. The method of claim 11 wherein said method further includes using cosmetically or pharmaceutically acceptable ingredients to formulate a product of cosmetic or pharmaceutical comprising at least one or full components of said crude extract.

13. The method of claim 11 wherein said method of protecting normal skin, or treating dry, chapped or cracked skin includes the topical application to the skin or scalp of a composition comprising at least one component of said crude extract.

14. The method of claim 11 wherein said method of accelerating wound healing includes the topical application to the wound of a composition comprising at least one component of said crude extract.
Page 11 of 13

15. The method of claim 11 wherein said method of peeling cells of stratum corneum or treating ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skim, etc includes the topical application to the skin or scalp of a compositions comprising at least one component of said crude extract.

16. The method of claim 11 wherein said method of making skin to have a silky smooth feeling includes the topical application to the skin of a composition comprising at least one component of said crude extract.

17. The method of claim 11 wherein said method of improving tissue cell metabolism, promoting cell turnover or enhancing skin, scalp, or wound quality includes the topical application to the skin, scalp, or wound of a composition comprising at least one component of said crude extract.

18. The method of claim 11 wherein said method of safely peeling cells of stratum corneum or treating ichthyosis, psoriasis, seborrheic keratoses, acne, dandruff, callous skin, etc includes the topical application to the skin or scalp of a composition comprising pancreatin of said crude extract.

19. The method of claim 11 wherein said method of protecting skin or scalp from aging includes the topical application to the skin of a composition comprising at least one component of said crude extract.
Page 12 of 13