CA2294916A1 - Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique - Google Patents

Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique Download PDF

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Publication number
CA2294916A1
CA2294916A1 CA002294916A CA2294916A CA2294916A1 CA 2294916 A1 CA2294916 A1 CA 2294916A1 CA 002294916 A CA002294916 A CA 002294916A CA 2294916 A CA2294916 A CA 2294916A CA 2294916 A1 CA2294916 A1 CA 2294916A1
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CA
Canada
Prior art keywords
cells
cell
disease
offspring
differentiated
Prior art date
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Abandoned
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CA002294916A
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English (en)
Inventor
Steven L. Stice
Jose Cibelli
James M. Robl
Paul Golueke
F. Abel Ponce De Leon
D. Joseph Jerry
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UMass Amherst
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Individual
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Publication of CA2294916A1 publication Critical patent/CA2294916A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Neurology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Neurosurgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Psychology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Diabetes (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Epidemiology (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Psychiatry (AREA)
  • Rheumatology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Hospice & Palliative Care (AREA)

Abstract

La présente invention concerne un procédé amélioré de transfert nucléaire consistant à la transplanter des noyaux de cellules donneuses différenciées à partir de cellules ne présentant pas de carence en sérum dans des ovocytes énucléés de même espèce que la cellule donneuse. Les unités de transfert nucléaire résultantes sont utilisées pour la multiplication de génotypes et de génotypes transgéniques par production de foetus et de progéniture et pour la production de cellules isogéniques à bouton embryonnaire cultivé (CICM), notamment de cellules souche ou de cellules embryonnaires isogéniques humaines. La production d'embryons, de foetus, et de progéniture de mammifères, transgéniques ou mis au point par génie génétique, est facilitée par ce procédé dans la mesuré où la source de cellules différenciées des noyaux donneurs peut être génétiquement modifiée et multipliée par clonage.
CA002294916A 1997-07-03 1998-06-24 Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique Abandoned CA2294916A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US88828397A 1997-07-03 1997-07-03
US08/888,283 1997-07-03
PCT/US1998/012800 WO1999001163A1 (fr) 1997-07-03 1998-06-24 Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique

Publications (1)

Publication Number Publication Date
CA2294916A1 true CA2294916A1 (fr) 1999-01-14

Family

ID=25392916

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002294916A Abandoned CA2294916A1 (fr) 1997-07-03 1998-06-24 Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique

Country Status (9)

Country Link
EP (1) EP1017422A4 (fr)
JP (1) JP2001509361A (fr)
CN (2) CN1265599A (fr)
AU (1) AU8154398A (fr)
BR (1) BR9811657A (fr)
CA (1) CA2294916A1 (fr)
IL (1) IL133786A0 (fr)
NZ (1) NZ502129A (fr)
WO (1) WO1999001163A1 (fr)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
WO1999036510A1 (fr) * 1998-01-16 1999-07-22 Agrobiogen Gmbh Transfert de noyau efficace avec des fibroblastes foetaux
US6331659B1 (en) 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
JP2002511234A (ja) * 1998-03-16 2002-04-16 リレグ ピーティーワイ リミテッド ブタの核移植
AUPP294898A0 (en) * 1998-04-15 1998-05-07 Monash University Method of nuclear transfer
EP1375654A3 (fr) * 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Mammifères trangéniques et clonés
EP1818397A1 (fr) * 1998-11-02 2007-08-15 Trustees Of Tufts College Procéde pour le clonage des animaux
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
WO2000025578A2 (fr) * 1999-04-26 2000-05-11 Trustees Of Tufts College Procedes de clonage des animaux
ES2338631T3 (es) 1999-03-04 2010-05-11 Revivicor, Inc. Modificacion genetica de celulas somaticas y usos de las mismas.
NZ508739A (en) * 1999-06-30 2002-11-26 Woo Suk Hwang Method for producing cloned tigers by employing inter-species nuclear transplantation technique
JP3652308B2 (ja) * 1999-06-30 2005-05-25 ソウル ナショナル ユニバーシティー インダストリー ファウンデーション クローンウシの生産方法
AU5577600A (en) * 1999-06-30 2001-01-31 Woo-Suk Hwang A method for producing human embryonic skin cells by employing an inter-species nuclear transplantation technique
CN1413250A (zh) * 1999-10-15 2003-04-23 先进细胞技术公司 用于生成分化祖细胞和谱系缺陷型胚胎干细胞的方法
DE10084883D2 (de) * 2000-06-08 2004-04-29 Trostner Jens Verfahren zur genetischen Rekonstruktion von menschlichen Organen
AU8470301A (en) 2000-08-03 2002-02-18 Wim-Van Schooten Production of humanized antibodies in transgenic animals
FR2814642B1 (fr) 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
US20020168763A1 (en) * 2000-11-30 2002-11-14 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
ATE466089T1 (de) 2000-12-22 2010-05-15 Agronomique Inst Nat Rech Positionsunabhängige und gewebespezifische expression eines transgens in der milch eines transgen-tieres
EP1356035B1 (fr) * 2000-12-22 2011-03-16 Kyowa Hakko Kirin Co., Ltd. Methodes de clonage de mammiferes non humains a l'aide de chromatine donneuse reprogrammee ou de cellules donneuses reprogrammees
JP4381139B2 (ja) 2001-08-13 2009-12-09 エンブレクス，インコーポレイテッド 鳥類の卵の処理方法
EP1490487A4 (fr) * 2002-04-01 2007-08-29 Gtc Biotherapeutics Inc Procede permettant la selection de lignees cellulaires convenant pour le transfert de noyau chez les especes mammiferes
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
PE20081216A1 (es) 2006-09-01 2008-09-04 Therapeutic Human Polyclonals Inc Expresion mejorada de la inmunoglobulina humana o humanizada en animales transgenicos no humanos
EP2267153A1 (fr) 2009-05-26 2010-12-29 Université Claude Bernard Lyon 1 Identification de la mutation du gène unc5c du récepteur de la nétrine-1 dans des cancers solides
WO2013010045A1 (fr) 2011-07-12 2013-01-17 Biotime Inc. Nouveaux procédés et formulations pour thérapie cellulaire orthopédique

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1595195A (en) * 1993-12-23 1995-07-10 Abs Global, Inc. Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
WO1999036510A1 (fr) * 1998-01-16 1999-07-22 Agrobiogen Gmbh Transfert de noyau efficace avec des fibroblastes foetaux

Also Published As

Publication number Publication date
NZ502129A (en) 2005-02-25
EP1017422A4 (fr) 2002-10-29
CN1265599A (zh) 2000-09-06
CN101003800A (zh) 2007-07-25
AU8154398A (en) 1999-01-25
JP2001509361A (ja) 2001-07-24
IL133786A0 (en) 2001-04-30
WO1999001163A1 (fr) 1999-01-14
EP1017422A1 (fr) 2000-07-12
BR9811657A (pt) 2000-09-05

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