CA2290510A1 - Method and kit for detecting dna mutations using restriction enzymes - Google Patents

Method and kit for detecting dna mutations using restriction enzymes Download PDF

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Publication number
CA2290510A1
CA2290510A1 CA002290510A CA2290510A CA2290510A1 CA 2290510 A1 CA2290510 A1 CA 2290510A1 CA 002290510 A CA002290510 A CA 002290510A CA 2290510 A CA2290510 A CA 2290510A CA 2290510 A1 CA2290510 A1 CA 2290510A1
Authority
CA
Canada
Prior art keywords
primers
detection
label
supports
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002290510A
Other languages
English (en)
French (fr)
Inventor
Ursula Bilitewski
Dirk Kuhlmeier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2290510A1 publication Critical patent/CA2290510A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002290510A 1997-05-21 1997-09-10 Method and kit for detecting dna mutations using restriction enzymes Abandoned CA2290510A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE19721327 1997-05-21
DE19739612.7 1997-09-09
DE19739612 1997-09-09
DE19721327.8 1997-09-09
PCT/EP1997/004955 WO1998053098A1 (de) 1997-05-21 1997-09-10 Verfahren und kit zur detektion von mutationen in dna's mit hilfe von restriktionsenzymen

Publications (1)

Publication Number Publication Date
CA2290510A1 true CA2290510A1 (en) 1998-11-26

Family

ID=26036720

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002290510A Abandoned CA2290510A1 (en) 1997-05-21 1997-09-10 Method and kit for detecting dna mutations using restriction enzymes

Country Status (6)

Country Link
EP (1) EP0983379A1 (ja)
JP (1) JP2001525678A (ja)
AU (1) AU737771B2 (ja)
CA (1) CA2290510A1 (ja)
RU (1) RU2193069C2 (ja)
WO (1) WO1998053098A1 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPR221400A0 (en) 2000-12-20 2001-01-25 Murdoch Childrens Research Institute, The Diagnostic assay
EP1618208B1 (en) * 2003-03-19 2010-04-28 The University of British Columbia Plasminogen activator inhibitor-1 (pai-1) haplotypes useful as indicators of patient outcome

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
WO1991017262A1 (en) * 1990-04-30 1991-11-14 The University Of British Columbia Method of establishing identity
CA2143428A1 (en) * 1993-07-09 1995-01-19 Takanori Oka Method of nucleic acid-differentiation and assay kit for nucleic acid-differentiation
WO1995025538A1 (en) * 1994-03-18 1995-09-28 The General Hospital Corporation Cleaved amplified rflp detection methods
FR2718461B1 (fr) * 1994-04-07 1996-05-15 Cis Bio Int Procédé de détection d'un site de restriction dans une séquence d'ADN.
US5851770A (en) * 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
DE69605803T2 (de) * 1995-05-11 2000-07-20 Ulf Landegren Nachweis von fehlpaarungen durch spaltung mit resolvase auf einem festträger
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods

Also Published As

Publication number Publication date
AU4302297A (en) 1998-12-11
EP0983379A1 (de) 2000-03-08
AU737771B2 (en) 2001-08-30
WO1998053098A1 (de) 1998-11-26
RU2193069C2 (ru) 2002-11-20
JP2001525678A (ja) 2001-12-11

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Legal Events

Date Code Title Description
FZDE Discontinued