AU737771B2 - Method and kit for the detection of mutations in DNA's using restriction enzymes - Google Patents
Method and kit for the detection of mutations in DNA's using restriction enzymes Download PDFInfo
- Publication number
- AU737771B2 AU737771B2 AU43022/97A AU4302297A AU737771B2 AU 737771 B2 AU737771 B2 AU 737771B2 AU 43022/97 A AU43022/97 A AU 43022/97A AU 4302297 A AU4302297 A AU 4302297A AU 737771 B2 AU737771 B2 AU 737771B2
- Authority
- AU
- Australia
- Prior art keywords
- primers
- detection
- supports
- label
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19721327 | 1997-05-21 | ||
DE19721327 | 1997-05-21 | ||
DE19739612 | 1997-09-09 | ||
DE19739612 | 1997-09-09 | ||
PCT/EP1997/004955 WO1998053098A1 (de) | 1997-05-21 | 1997-09-10 | Verfahren und kit zur detektion von mutationen in dna's mit hilfe von restriktionsenzymen |
Publications (2)
Publication Number | Publication Date |
---|---|
AU4302297A AU4302297A (en) | 1998-12-11 |
AU737771B2 true AU737771B2 (en) | 2001-08-30 |
Family
ID=26036720
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU43022/97A Ceased AU737771B2 (en) | 1997-05-21 | 1997-09-10 | Method and kit for the detection of mutations in DNA's using restriction enzymes |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0983379A1 (ja) |
JP (1) | JP2001525678A (ja) |
AU (1) | AU737771B2 (ja) |
CA (1) | CA2290510A1 (ja) |
RU (1) | RU2193069C2 (ja) |
WO (1) | WO1998053098A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPR221400A0 (en) | 2000-12-20 | 2001-01-25 | Murdoch Childrens Research Institute, The | Diagnostic assay |
CA2519362A1 (en) * | 2003-03-19 | 2004-09-30 | The University Of British Columbia | Plasminogen activator inhibitor-1 (pai-1) haplotypes useful as indicators of patient outcome |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991017262A1 (en) * | 1990-04-30 | 1991-11-14 | The University Of British Columbia | Method of establishing identity |
WO1995025538A1 (en) * | 1994-03-18 | 1995-09-28 | The General Hospital Corporation | Cleaved amplified rflp detection methods |
WO1997009444A1 (en) * | 1995-09-01 | 1997-03-13 | Variagenics, Inc. | Detection of mismatches by resolvase cleavage using a magnetic bead support |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
EP0664339A4 (en) * | 1993-07-09 | 1999-04-28 | Wakunaga Seiyaku Kk | METHOD FOR DISCRIMINATION OF NUCLEIC ACIDS AND TEST KIT FOR THIS PURPOSE. |
FR2718461B1 (fr) * | 1994-04-07 | 1996-05-15 | Cis Bio Int | Procédé de détection d'un site de restriction dans une séquence d'ADN. |
WO1996035809A1 (en) * | 1995-05-11 | 1996-11-14 | Avitech Diagnostics Inc | Detection of mismatches by resolvase cleavage on a solid support |
US5753439A (en) * | 1995-05-19 | 1998-05-19 | Trustees Of Boston University | Nucleic acid detection methods |
-
1997
- 1997-09-10 EP EP97919050A patent/EP0983379A1/de not_active Withdrawn
- 1997-09-10 RU RU99127314/13A patent/RU2193069C2/ru not_active IP Right Cessation
- 1997-09-10 WO PCT/EP1997/004955 patent/WO1998053098A1/de not_active Application Discontinuation
- 1997-09-10 JP JP54983598A patent/JP2001525678A/ja active Pending
- 1997-09-10 AU AU43022/97A patent/AU737771B2/en not_active Ceased
- 1997-09-10 CA CA002290510A patent/CA2290510A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991017262A1 (en) * | 1990-04-30 | 1991-11-14 | The University Of British Columbia | Method of establishing identity |
WO1995025538A1 (en) * | 1994-03-18 | 1995-09-28 | The General Hospital Corporation | Cleaved amplified rflp detection methods |
WO1997009444A1 (en) * | 1995-09-01 | 1997-03-13 | Variagenics, Inc. | Detection of mismatches by resolvase cleavage using a magnetic bead support |
Also Published As
Publication number | Publication date |
---|---|
AU4302297A (en) | 1998-12-11 |
EP0983379A1 (de) | 2000-03-08 |
CA2290510A1 (en) | 1998-11-26 |
RU2193069C2 (ru) | 2002-11-20 |
WO1998053098A1 (de) | 1998-11-26 |
JP2001525678A (ja) | 2001-12-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4216333B2 (ja) | オリゴヌクレオチドの化学結合による核酸検出及び増幅 | |
AU2008217678B9 (en) | Method and test kit for determining the amounts of target sequences and nucleotide variations therein | |
Nikiforov et al. | Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms | |
US6004783A (en) | Cleaved amplified RFLP detection methods | |
JP3637308B2 (ja) | Dna試料中のシトシン塩基のメチル化度の相対的定量方法 | |
KR101041106B1 (ko) | 핵산과 단백질의 신규한 실시간 탐지방법 | |
JP2000510710A (ja) | ジスルフィド結合による核酸分子の固相への共有結合による固定化 | |
AU5572099A (en) | Fluorescence polarization in nucleic acid analysis | |
JP2003512808A (ja) | Pcrを使用する標的となる核酸を検出する方法 | |
JP2002518060A (ja) | ヌクレオチド検出法 | |
JP2004521634A (ja) | MutSおよびRecAを使用する変異検出 | |
US5741637A (en) | Process for the production of modified nucleic acids | |
Gibson et al. | A colorimetric assay for phosphate to measure amplicon accumulation in polymerase chain reaction | |
JP2006508656A (ja) | 診断ハイブリダイゼーションアッセイにおける配列変異の影響及び基線上昇の作用の両方を低下させるための方法、このような方法を実施するためのアッセイ及び前記アッセイにおける使用のためのプローブ | |
AU737771B2 (en) | Method and kit for the detection of mutations in DNA's using restriction enzymes | |
JP4628625B2 (ja) | 蛍光エネルギー移動を含む標的核酸を検出するための増幅方法 | |
WO2018049260A1 (en) | Reusable microarray compositions and methods | |
JP2673162B2 (ja) | 核酸の高感度検出方法 | |
EP1114185B1 (en) | Method of isolating primer extension products with modular oligonucleotides | |
EP0406280A1 (en) | Method for amplifying and detecting nucleic acid in a test liquid | |
WO2023025218A1 (zh) | 一种用于检测单碱基突变的引物组和基因芯片方法 | |
JP2905192B2 (ja) | 遺伝子発現の定量方法 | |
US20080090235A1 (en) | Unstructured nucleic acid PCR primers and methods of using the same | |
RU2247781C2 (ru) | Способ определения однонуклеотидных замен в известных последовательностях нуклеиновых кислот | |
JPH0646899A (ja) | 核酸結合因子の検出のための迅速な検定 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FGA | Letters patent sealed or granted (standard patent) | ||
MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |