CA2286156C - Method of harvesting rare cells from blood products - Google Patents
Method of harvesting rare cells from blood products Download PDFInfo
- Publication number
- CA2286156C CA2286156C CA2286156A CA2286156A CA2286156C CA 2286156 C CA2286156 C CA 2286156C CA 2286156 A CA2286156 A CA 2286156A CA 2286156 A CA2286156 A CA 2286156A CA 2286156 C CA2286156 C CA 2286156C
- Authority
- CA
- Canada
- Prior art keywords
- porous medium
- cells
- rare cells
- elution fluid
- rare
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 135
- 239000010836 blood and blood product Substances 0.000 title claims abstract description 72
- 229940125691 blood product Drugs 0.000 title claims abstract description 71
- 238000003306 harvesting Methods 0.000 title claims abstract description 25
- 239000012530 fluid Substances 0.000 claims abstract description 135
- 238000010828 elution Methods 0.000 claims abstract description 131
- 230000000717 retained effect Effects 0.000 claims abstract description 49
- 210000004027 cell Anatomy 0.000 claims description 327
- 210000000265 leukocyte Anatomy 0.000 claims description 42
- 210000004698 lymphocyte Anatomy 0.000 claims description 25
- 210000003714 granulocyte Anatomy 0.000 claims description 24
- 210000004443 dendritic cell Anatomy 0.000 claims description 17
- 210000000130 stem cell Anatomy 0.000 claims description 17
- 150000001768 cations Chemical class 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 14
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 12
- 229920000642 polymer Polymers 0.000 claims description 11
- 229940098773 bovine serum albumin Drugs 0.000 claims description 7
- 230000000295 complement effect Effects 0.000 claims description 7
- 235000000346 sugar Nutrition 0.000 claims description 7
- IJRKANNOPXMZSG-SSPAHAAFSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O.OC(=O)CC(O)(C(O)=O)CC(O)=O IJRKANNOPXMZSG-SSPAHAAFSA-N 0.000 claims description 6
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- FZWBNHMXJMCXLU-UHFFFAOYSA-N 2,3,4,5-tetrahydroxy-6-[3,4,5-trihydroxy-6-[[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxyhexanal Chemical compound OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OCC(O)C(O)C(O)C(O)C=O)O1 FZWBNHMXJMCXLU-UHFFFAOYSA-N 0.000 description 2
- RSGFPIWWSCWCFJ-VAXZQHAWSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;phosphoric acid Chemical compound OP(O)(O)=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O.OC(=O)CC(O)(C(O)=O)CC(O)=O RSGFPIWWSCWCFJ-VAXZQHAWSA-N 0.000 description 2
- 231100000699 Bacterial toxin Toxicity 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108091006905 Human Serum Albumin Proteins 0.000 description 2
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- 239000004952 Polyamide Substances 0.000 description 2
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
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- GZCGUPFRVQAUEE-KVTDHHQDSA-N aldehydo-D-mannose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-KVTDHHQDSA-N 0.000 description 2
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
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- 229940119743 dextran 70 Drugs 0.000 description 2
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- 239000003112 inhibitor Substances 0.000 description 2
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- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 description 2
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- 239000003634 thrombocyte concentrate Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 206010051392 Diapedesis Diseases 0.000 description 1
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- JHWNWJKBPDFINM-UHFFFAOYSA-N Laurolactam Chemical compound O=C1CCCCCCCCCCCN1 JHWNWJKBPDFINM-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
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- 230000001464 adherent effect Effects 0.000 description 1
- GZCGUPFRVQAUEE-KCDKBNATSA-N aldehydo-D-galactose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-KCDKBNATSA-N 0.000 description 1
- 230000005167 amoeboid movement Effects 0.000 description 1
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- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
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- 210000000601 blood cell Anatomy 0.000 description 1
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- 230000020411 cell activation Effects 0.000 description 1
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- 238000005119 centrifugation Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
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- 239000007979 citrate buffer Substances 0.000 description 1
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- 229940119744 dextran 40 Drugs 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
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- XEYBRNLFEZDVAW-ARSRFYASSA-N dinoprostone Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1C\C=C/CCCC(O)=O XEYBRNLFEZDVAW-ARSRFYASSA-N 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 150000004676 glycans Chemical class 0.000 description 1
- 230000018981 granulocyte chemotaxis Effects 0.000 description 1
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- 239000000041 non-steroidal anti-inflammatory agent Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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- 230000000704 physical effect Effects 0.000 description 1
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- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4320097P | 1997-04-08 | 1997-04-08 | |
| US60/043,200 | 1997-04-08 | ||
| US6411197P | 1997-11-03 | 1997-11-03 | |
| US60/064,111 | 1997-11-03 | ||
| US6419297P | 1997-11-04 | 1997-11-04 | |
| US60/064,192 | 1997-11-04 | ||
| PCT/US1998/006643 WO1998045413A1 (en) | 1997-04-08 | 1998-04-03 | Method of harvesting rare cells from blood products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2286156A1 CA2286156A1 (en) | 1998-10-15 |
| CA2286156C true CA2286156C (en) | 2013-03-19 |
Family
ID=27366293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2286156A Expired - Lifetime CA2286156C (en) | 1997-04-08 | 1998-04-03 | Method of harvesting rare cells from blood products |
Country Status (8)
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040224300A1 (en) * | 1997-01-24 | 2004-11-11 | Shuji Terashima | Method for separating nucleated cells |
| EP1690930A1 (en) * | 1997-04-08 | 2006-08-16 | Pall Corporation | Method of harvesting rare cells from blood products |
| US7745106B2 (en) * | 1997-06-24 | 2010-06-29 | Cascade Medical Enterprises, Llc | Methods and devices for separating liquid components |
| US6979307B2 (en) * | 1997-06-24 | 2005-12-27 | Cascade Medical Enterprises Llc | Systems and methods for preparing autologous fibrin glue |
| EP1916297B1 (en) * | 2001-04-10 | 2010-12-29 | BioE LLC | Cell separation compositions and methods |
| US7074440B2 (en) * | 2002-05-01 | 2006-07-11 | Rulin Xiu | Compositions and methods for body weight loss |
| ES2523366T3 (es) * | 2002-06-19 | 2014-11-25 | Northwest Biotherapeutics, Inc. | Uso de un dispositivo de filtración de flujo tangencial y métodos para el enriquecimiento de leucocitos |
| US7291450B2 (en) * | 2003-03-28 | 2007-11-06 | Smith & Nephew, Inc. | Preparation of a cell concentrate from a physiological solution |
| US20050208501A1 (en) | 2004-03-16 | 2005-09-22 | Ambion, Inc. | Process and reagents for extraction of RNA from fractionated blood leukocytes |
| US20060234210A1 (en) * | 2004-04-14 | 2006-10-19 | Affinergy, Inc. | Filtration device and method for removing selected materials from biological fluids |
| CA2579041A1 (en) * | 2004-09-07 | 2006-03-16 | Smith & Nephew, Inc. | Methods and devices for sterile field transfer |
| WO2006058153A1 (en) * | 2004-11-23 | 2006-06-01 | Smith & Nephew, Inc. | Composite mixer |
| CA2592381A1 (en) * | 2004-12-16 | 2006-06-22 | Idexx Laboratories, Inc. | Apparatus and method to elute microorganisms from a filter |
| EP1846163A2 (en) * | 2005-01-13 | 2007-10-24 | Micronics, Inc. | Microfluidic rare cell detection device |
| WO2007100986A2 (en) * | 2006-02-24 | 2007-09-07 | Rosetta Inpharmatics Llc | Extraction and diagnostic fluid devices, systems and methods of use |
| US7598089B2 (en) * | 2006-04-19 | 2009-10-06 | Bioe, Inc. | Methods and compositions for separating cells |
| US7713688B2 (en) * | 2007-01-26 | 2010-05-11 | Bioe, Llc | Methods and compositions for depleting specific cell populations from blood tissues |
| CA2679042C (en) | 2007-03-02 | 2015-11-24 | Smith & Nephew Plc | Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples |
| US8546127B2 (en) * | 2008-06-30 | 2013-10-01 | General Electric Company | Bacteria/RNA extraction device |
| US8158405B2 (en) * | 2008-06-30 | 2012-04-17 | General Electric Company | Process for concentrating and processing fluid samples |
| US20100159506A1 (en) * | 2008-07-25 | 2010-06-24 | Cellscape Corporation | Methods and systems for genetic analysis of fetal nucleated red blood cells |
| US8961787B2 (en) | 2008-12-01 | 2015-02-24 | General Electric Company | Systems and methods for processing complex biological materials |
| US20100151438A1 (en) * | 2008-12-12 | 2010-06-17 | General Electric Company | Methods and kits for enhancing sedimentation and recovery of cells in a sample |
| US20100210989A1 (en) | 2008-12-23 | 2010-08-19 | Janet Lesley Macpherson | Processing blood |
| WO2011001936A1 (ja) | 2009-06-30 | 2011-01-06 | 株式会社カネカ | 血液成分の分離システム、分離材 |
| US9034280B2 (en) * | 2009-12-16 | 2015-05-19 | General Electric Corporation | High-throughput methods and systems for processing biological materials |
| EP2363501A1 (en) * | 2010-03-02 | 2011-09-07 | Universitätsklinikum Hamburg-Eppendorf | Method for isolating target cells |
| US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
| US20130323712A1 (en) | 2010-11-25 | 2013-12-05 | Kaneka Corporation | Method and material for separating leukocytes or mononuclear cells |
| JP2012120458A (ja) * | 2010-12-06 | 2012-06-28 | Kaneka Corp | 細胞の分離器具。 |
| US20130143195A1 (en) * | 2011-12-05 | 2013-06-06 | Pall Corporation | Leukocyte purification |
| US9427512B2 (en) | 2012-06-08 | 2016-08-30 | Pall Corporation | Filter device |
| US9421317B2 (en) * | 2012-06-08 | 2016-08-23 | Pall Corporation | Cell harvesting device and system |
| GB201306810D0 (en) | 2013-04-15 | 2013-05-29 | Cells4Life Group Llp | Methods of cell separation |
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1998
- 1998-04-03 EP EP06075344A patent/EP1690930A1/en not_active Withdrawn
- 1998-04-03 CA CA2286156A patent/CA2286156C/en not_active Expired - Lifetime
- 1998-04-03 AU AU67954/98A patent/AU6795498A/en not_active Abandoned
- 1998-04-03 EP EP98913392A patent/EP0973873B1/en not_active Expired - Lifetime
- 1998-04-03 WO PCT/US1998/006643 patent/WO1998045413A1/en active IP Right Grant
- 1998-04-03 US US09/402,158 patent/US6544751B1/en not_active Expired - Lifetime
- 1998-04-03 DE DE69834809T patent/DE69834809T2/de not_active Expired - Lifetime
- 1998-04-03 JP JP54293298A patent/JP4187275B2/ja not_active Expired - Lifetime
- 1998-04-03 AT AT98913392T patent/ATE329012T1/de active
-
2003
- 2003-02-11 US US10/361,586 patent/US20030134417A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001518792A (ja) | 2001-10-16 |
| US20030134417A1 (en) | 2003-07-17 |
| DE69834809D1 (de) | 2006-07-20 |
| AU6795498A (en) | 1998-10-30 |
| DE69834809T2 (de) | 2007-05-16 |
| JP4187275B2 (ja) | 2008-11-26 |
| EP0973873B1 (en) | 2006-06-07 |
| US6544751B1 (en) | 2003-04-08 |
| EP0973873A1 (en) | 2000-01-26 |
| ATE329012T1 (de) | 2006-06-15 |
| WO1998045413A1 (en) | 1998-10-15 |
| CA2286156A1 (en) | 1998-10-15 |
| EP1690930A1 (en) | 2006-08-16 |
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