CA2271068A1 - Procede d'identification des genes regules par traduction - Google Patents

Procede d'identification des genes regules par traduction Download PDF

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Publication number
CA2271068A1
CA2271068A1 CA002271068A CA2271068A CA2271068A1 CA 2271068 A1 CA2271068 A1 CA 2271068A1 CA 002271068 A CA002271068 A CA 002271068A CA 2271068 A CA2271068 A CA 2271068A CA 2271068 A1 CA2271068 A1 CA 2271068A1
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CA
Canada
Prior art keywords
mrna
set forth
genes
translation
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002271068A
Other languages
English (en)
Inventor
Zehava Grosman
Paz Einat
Nicholas Harris
Rami Skaliter
Sylvie Luria
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
QBI Enterprises Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/748,130 external-priority patent/US6013437A/en
Application filed by QBI Enterprises Ltd filed Critical QBI Enterprises Ltd
Publication of CA2271068A1 publication Critical patent/CA2271068A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé d'identification des gènes régulées par traduction, consistant à stimuler sélectivement la traduction d'un ARNm cible inconnu à l'aide d'un élément inducteur de stress, l'ARNm cible inconnu faisant partie d'un échantillon plus important d'ARNm. L'échantillon d'ARNm se divise en amas traduits et non traduits d'ARNm qui sont analysés différentiellement pour identifier les gènes régulés par traduction à l'aide de l'élément inducteur de stress. L'invention porte également sur un procédé d'identification des séquences géniques codant pour les sites internes d'entrée des ribosomes, consistant à inhiber dans une cellule la traduction de l'ARNm dépendant de la région 5' à coiffe, à recueillir un amas d'ARNm à partir des cellules, puis à effectuer une analyse différentielle de l'amas d'ARNm afin d'identifier les gènes présentant des séquences codant pour des sites internes d'entrée des ribosomes.
CA002271068A 1996-11-12 1997-11-12 Procede d'identification des genes regules par traduction Abandoned CA2271068A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/748,130 US6013437A (en) 1996-11-12 1996-11-12 Method for identifying translationally regulated genes
US94358697A 1997-10-03 1997-10-03
US08/748,130 1997-10-03
US08/943,586 1997-10-03
PCT/US1997/020831 WO1998021321A1 (fr) 1996-11-12 1997-11-12 Procede d'identification des genes regules par traduction

Publications (1)

Publication Number Publication Date
CA2271068A1 true CA2271068A1 (fr) 1998-05-22

Family

ID=27114885

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002271068A Abandoned CA2271068A1 (fr) 1996-11-12 1997-11-12 Procede d'identification des genes regules par traduction

Country Status (6)

Country Link
EP (1) EP0942969A1 (fr)
JP (1) JP2002515754A (fr)
AU (1) AU5258098A (fr)
CA (1) CA2271068A1 (fr)
IL (2) IL129872A0 (fr)
WO (1) WO1998021321A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037189A1 (fr) 1997-02-25 1998-08-27 Qbi Enterprises Ltd. Sequences d'ires conferant une grande efficacite de traduction et vecteurs d'expression contenant la sequence
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US6171821B1 (en) 1998-07-24 2001-01-09 Apoptogen, Inc. XIAP IRES and uses thereof
EP1147188B1 (fr) * 1999-01-26 2007-05-16 Vlaams Interuniversitair Instituut voor Biotechnologie vzw. Site d'entree ribosomique interne (ires), vecteur le contenant et utilisations correspondantes
US6764852B2 (en) 1999-01-26 2004-07-20 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Internal ribosome entry site, vector containing same and uses thereof
JP2003524397A (ja) * 1999-03-16 2003-08-19 マイトコー オルガネラ遺伝子産物の示差的発現
US6403316B1 (en) * 1999-03-24 2002-06-11 Quark Biotech, Inc. Isolation of cDNA encoding for secreted or membranal proteins
AU4224400A (en) * 1999-04-08 2000-10-23 Sir Mortimer B. Davis Jewish General Hospital Quantitative assay for expression of genes in microarray
AU4562700A (en) * 1999-05-05 2000-11-21 European Molecular Biology Laboratory Improved predictive power of rna analysis for protein expression
AU2001251086A1 (en) * 2000-03-31 2001-10-15 Sir Mortimer B. Davis Jewish General Hospital Microchip arrays of regulatory genes
JP4723713B2 (ja) * 2000-09-25 2011-07-13 トヨタ自動車株式会社 ｍＲＮＡの潜在的翻訳制御因子のスクリーニング方法
WO2002042500A2 (fr) 2000-10-31 2002-05-30 Hitachi Chemical Research Center, Inc. Appareil et procede pour la concentration electrophoretique sur micropoint
US7374881B2 (en) 2000-10-31 2008-05-20 Hitachi Chemical Research Center, Inc. Method for collecting and using nuclear mRNA
KR20020096559A (ko) * 2001-06-21 2002-12-31 이준호 알코올에 의해 특이적으로 그 전사발현이 유도되거나저해되는 알코올 민감성 유전자 발굴
BE1014949A3 (fr) 2001-08-14 2004-07-06 Probiox Procede de detection de stress oxydant et trousse pour sa mise en oeuvre.
WO2011021666A1 (fr) 2009-08-19 2011-02-24 国立大学法人奈良先端科学技術大学院大学 Molécule d'adn recombinant codant pour une région 5'utr évitant la suppression de traduction sous stress environnemental
WO2012009644A2 (fr) * 2010-07-16 2012-01-19 Arizona Board Of Regents Procédés pour identifier des éléments d'arn synthétiques et naturels qui améliorent la traduction des protéines
JP6037339B2 (ja) * 2011-09-02 2016-12-07 国立大学法人 奈良先端科学技術大学院大学 形質転換植物細胞を用いたタンパク質製造方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations

Also Published As

Publication number Publication date
WO1998021321A1 (fr) 1998-05-22
AU5258098A (en) 1998-06-03
IL129872A (en) 2006-04-10
EP0942969A1 (fr) 1999-09-22
JP2002515754A (ja) 2002-05-28
IL129872A0 (en) 2000-02-29

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Legal Events

Date Code Title Description
FZDE Discontinued