CA2262751A1 - Activateur du plasminogene activable par thrombine - Google Patents

Activateur du plasminogene activable par thrombine Download PDF

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Publication number
CA2262751A1
CA2262751A1 CA002262751A CA2262751A CA2262751A1 CA 2262751 A1 CA2262751 A1 CA 2262751A1 CA 002262751 A CA002262751 A CA 002262751A CA 2262751 A CA2262751 A CA 2262751A CA 2262751 A1 CA2262751 A1 CA 2262751A1
Authority
CA
Canada
Prior art keywords
plasminogen activator
thrombin
plasmin
amino acids
plasminogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002262751A
Other languages
English (en)
Inventor
Ulrich Kohnert
Stephan Fischer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2262751A1 publication Critical patent/CA2262751A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un activateur du plasminogène (t-PA) qui a) est modifié de manière que l'activateur du plasminogène soit clivable par thrombine et soit transformé dans la forme à deux chaînes suite à un tel clivage, b) est modifié de manière que comparativement à t-PA, la zymogénité soit supérieure d'au moins un facteur 1,2, et c) dont la liaison à la fibrine est réduite jusqu'à ce que plus de 50 % de l'activateur du plasminogène puisse pénétrer dans un caillot de sang. Cet activateur du plasminogène (t-PA) présente une spécificité fibrinique améliorée et des effets secondaires moindres.
CA002262751A 1996-08-02 1997-07-21 Activateur du plasminogene activable par thrombine Abandoned CA2262751A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP96112487.2 1996-08-02
EP96112487 1996-08-02
PCT/EP1997/003925 WO1998005762A1 (fr) 1996-08-02 1997-07-21 Activateur du plasminogene activable par thrombine

Publications (1)

Publication Number Publication Date
CA2262751A1 true CA2262751A1 (fr) 1998-02-12

Family

ID=8223069

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002262751A Abandoned CA2262751A1 (fr) 1996-08-02 1997-07-21 Activateur du plasminogene activable par thrombine

Country Status (11)

Country Link
EP (1) EP0917567A1 (fr)
JP (1) JP2000504941A (fr)
KR (1) KR20000029755A (fr)
CN (1) CN1226926A (fr)
AU (1) AU3769797A (fr)
BR (1) BR9711015A (fr)
CA (1) CA2262751A1 (fr)
TR (1) TR199900193T2 (fr)
TW (1) TW450996B (fr)
WO (1) WO1998005762A1 (fr)
ZA (1) ZA976876B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990081421A (ko) * 1998-04-29 1999-11-15 성재갑 동물세포를 이용한 인간 혈소판 생성 촉진인자(tpo)의 제조방법
CN101886066A (zh) * 2009-05-13 2010-11-17 南京大学 组织型纤溶酶原激活物环饼结构-2对脑卒中的治疗作用及制备方法
AU2018271424A1 (en) 2017-12-13 2019-06-27 Playable Pty Ltd System and Method for Algorithmic Editing of Video Content

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200340A (en) * 1987-05-22 1993-04-06 Zymogenetics, Inc. Thrombin-activated tissue plasminogen activators
EP0316068A1 (fr) * 1987-10-09 1989-05-17 Collaborative Research Inc. Activateur du plasminogène modifié de bas poids moléculaire et méthode pour sa préparation
US5504001A (en) * 1987-11-25 1996-04-02 Zymogenetics, Inc. Hybrid plasminogen activator
US4999194A (en) * 1988-01-14 1991-03-12 Collaborative Research, Inc. Two-chain urokinase plasminogen activators for treatment of thrombotic disease
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
GB9222758D0 (en) * 1992-10-29 1992-12-09 British Bio Technology Proteins and nucleic acids

Also Published As

Publication number Publication date
CN1226926A (zh) 1999-08-25
ZA976876B (en) 1999-02-01
TR199900193T2 (xx) 1999-03-22
KR20000029755A (ko) 2000-05-25
JP2000504941A (ja) 2000-04-25
TW450996B (en) 2001-08-21
AU3769797A (en) 1998-02-25
BR9711015A (pt) 1999-08-17
EP0917567A1 (fr) 1999-05-26
WO1998005762A1 (fr) 1998-02-12

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead