CA2249061C - Protein production process - Google Patents

Protein production process Download PDF

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Publication number
CA2249061C
CA2249061C CA002249061A CA2249061A CA2249061C CA 2249061 C CA2249061 C CA 2249061C CA 002249061 A CA002249061 A CA 002249061A CA 2249061 A CA2249061 A CA 2249061A CA 2249061 C CA2249061 C CA 2249061C
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CA
Canada
Prior art keywords
carbon source
enzyme
repressive
sugar
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002249061A
Other languages
English (en)
French (fr)
Other versions
CA2249061A1 (en
Inventor
Karsten Hellmuth
Rual Lopez-Ulibarri
Anne Francoise Mayer
Heinrich Winfried Schlieker
Adolphus Van Loon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CA2249061A1 publication Critical patent/CA2249061A1/en
Application granted granted Critical
Publication of CA2249061C publication Critical patent/CA2249061C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002249061A 1997-10-01 1998-09-29 Protein production process Expired - Lifetime CA2249061C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97117021.2 1997-10-01
EP97117021 1997-10-01

Publications (2)

Publication Number Publication Date
CA2249061A1 CA2249061A1 (en) 1999-04-01
CA2249061C true CA2249061C (en) 2009-02-24

Family

ID=8227420

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002249061A Expired - Lifetime CA2249061C (en) 1997-10-01 1998-09-29 Protein production process

Country Status (12)

Country Link
US (1) US6204012B1 (US06559137-20030506-C00021.png)
EP (2) EP1659173A1 (US06559137-20030506-C00021.png)
JP (1) JP4162774B2 (US06559137-20030506-C00021.png)
KR (1) KR100572426B1 (US06559137-20030506-C00021.png)
CN (1) CN1215175C (US06559137-20030506-C00021.png)
AU (1) AU748187B2 (US06559137-20030506-C00021.png)
BR (1) BR9803807B1 (US06559137-20030506-C00021.png)
CA (1) CA2249061C (US06559137-20030506-C00021.png)
DE (1) DE69834533T2 (US06559137-20030506-C00021.png)
DK (1) DK0911416T3 (US06559137-20030506-C00021.png)
ES (1) ES2264181T3 (US06559137-20030506-C00021.png)
IN (1) IN188151B (US06559137-20030506-C00021.png)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) * 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
CN100347303C (zh) * 1999-03-31 2007-11-07 康乃尔研究基金会有限公司 具有改良的肌醇六磷酸酶活性的磷酸酶
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
EP1450627B1 (en) 2001-10-31 2012-09-05 Phytex, Llc Use of phytase containing animal food
KR100429331B1 (ko) * 2002-01-29 2004-04-29 재단법인 목암생명공학연구소 재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법
CA2492267C (en) * 2002-07-15 2013-09-10 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
CA2925807C (en) 2002-09-13 2020-09-15 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
EP1660661A2 (en) * 2003-08-08 2006-05-31 Arriva Pharmaceuticals, Inc. Methods of protein production in yeast
CA2545855A1 (en) * 2003-11-14 2005-06-02 Baxter International Inc. Alpha 1-antitrypsin compositions and treatment methods using such compositions
EP2009093A3 (en) * 2004-09-22 2009-04-08 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
US20070105768A1 (en) * 2004-11-10 2007-05-10 Rajiv Nayar Dry recombinant human alpha 1-antitrypsin formulation
CN101287839B (zh) 2005-03-24 2015-04-08 帝斯曼知识产权资产管理有限公司 对有价值化合物进行微生物生产的方法
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
US7510857B2 (en) * 2006-04-28 2009-03-31 C5-6 Technologies, Inc. Thermostable cellulase and methods of use
EP2069486A2 (en) * 2006-08-03 2009-06-17 Cornell Research Foundation, Inc. Phytases with improved thermal stability
WO2008090211A1 (en) 2007-01-26 2008-07-31 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
WO2009137574A2 (en) * 2008-05-06 2009-11-12 Archer Daniels Midland Company Development of strains of the thermotolerant yeast hansenula polymorpha capable of alcoholic fermentation of starch and xylan by expression of starch and xylan degrading enzymes
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CN103159531A (zh) * 2013-04-17 2013-06-19 广西新方向化学工业有限公司 一种含多种植物醇和中微量元素螯合的液体肥料及其制备方法
AR095173A1 (es) 2013-07-25 2015-09-30 Basf Enzymes Llc Fitasa
EP3217807A4 (en) 2014-11-11 2018-09-12 Clara Foods Co. Methods and compositions for egg white protein production
CN104928271A (zh) * 2015-07-06 2015-09-23 唐亚军 一种木聚糖酶的制备方法
CN105200101B (zh) * 2015-10-19 2019-04-16 华中农业大学 一种毕赤酵母菌体重复发酵表达外源蛋白的方法
EP3997118A4 (en) 2019-07-11 2023-07-19 Clara Foods Co. PROTEIN COMPOSITIONS AND CONSUMABLE PRODUCTS THEREOF
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0173378B1 (en) 1984-07-27 1991-06-12 Unilever N.V. Process for preparing a polypeptide by culturing a transformed microorganism, a transformed microorganism suitable therefor and dna sequences suitable for preparing such microorganism
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
NL8601454A (nl) * 1986-06-05 1988-01-04 Unilever Nv Werkwijze voor het bereiden van een catalase-vrij oxidoreductase en van een catalase-vrije oxidoreductase bevattende gist, en gebruik daarvan.
ATE58169T1 (de) * 1986-11-24 1990-11-15 Unilever Nv Verfahren zur herstellung einer catalasefreien, oxidase enthaltenden hefe und deren verwendung.
EP0299108B1 (en) 1987-07-17 1994-05-18 Rhein Biotech Gesellschaft für biotechnologische Prozesse und Produkte mbH DNA-molecules coding for FMDH control regions and structured gene for a protein having FMDH-activity and their uses
JPH04501662A (ja) * 1988-09-26 1992-03-26 ザ・サルク・インスティチュート・バイオテクノロジー／インダストリアル・アソシエイツ・インコーポレーテッド 混合給送組換え酵母発酵
US5667986A (en) * 1989-10-18 1997-09-16 Delta Biotechnology Limited Yeast promoter for expressing heterologous polypeptides
AU657168B2 (en) * 1991-09-18 1995-03-02 Amgen, Inc. Hepatitis B vaccine with bile salt adjuvant
ES2268687T3 (es) 1994-04-25 2007-03-16 Dsm Ip Assets B.V. Polipeptidos con actividad fitasa.
US6068995A (en) * 1994-10-25 2000-05-30 Yoshitomi Pharmaceutical Industries, Ltd. Method for producing protein
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases

Also Published As

Publication number Publication date
AU748187B2 (en) 2002-05-30
EP0911416A2 (en) 1999-04-28
AU8715798A (en) 1999-04-22
IN188151B (US06559137-20030506-C00021.png) 2002-08-24
DK0911416T3 (da) 2006-08-14
ES2264181T3 (es) 2006-12-16
EP0911416A3 (en) 2000-01-19
KR19990036734A (ko) 1999-05-25
EP1659173A1 (en) 2006-05-24
JPH11169173A (ja) 1999-06-29
CA2249061A1 (en) 1999-04-01
KR100572426B1 (ko) 2006-06-21
US6204012B1 (en) 2001-03-20
CN1218112A (zh) 1999-06-02
DE69834533D1 (de) 2006-06-22
JP4162774B2 (ja) 2008-10-08
BR9803807A (pt) 2000-03-28
EP0911416B1 (en) 2006-05-17
DE69834533T2 (de) 2006-10-12
BR9803807B1 (pt) 2010-11-03
CN1215175C (zh) 2005-08-17

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Effective date: 20181001