CA2238434A1 - Method for obtaining retroviral vector supernatant having high transduction efficiency - Google Patents
Method for obtaining retroviral vector supernatant having high transduction efficiency Download PDFInfo
- Publication number
- CA2238434A1 CA2238434A1 CA 2238434 CA2238434A CA2238434A1 CA 2238434 A1 CA2238434 A1 CA 2238434A1 CA 2238434 CA2238434 CA 2238434 CA 2238434 A CA2238434 A CA 2238434A CA 2238434 A1 CA2238434 A1 CA 2238434A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- vector
- supernatant
- propak
- retroviral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13051—Methods of production or purification of viral material
- C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US57294395A | 1995-12-15 | 1995-12-15 | |
US08/572,943 | 1995-12-15 | ||
US08/572,959 US5910434A (en) | 1995-12-15 | 1995-12-15 | Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant |
US08/572,959 | 1995-12-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2238434A1 true CA2238434A1 (en) | 1997-06-19 |
Family
ID=27075989
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2238434 Abandoned CA2238434A1 (en) | 1995-12-15 | 1996-12-13 | Method for obtaining retroviral vector supernatant having high transduction efficiency |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0871753A2 (ja) |
JP (1) | JP2000501614A (ja) |
AU (1) | AU1333797A (ja) |
CA (1) | CA2238434A1 (ja) |
WO (1) | WO1997021824A2 (ja) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5994134A (en) * | 1998-05-04 | 1999-11-30 | Canji, Inc. | Viral production process |
US6790614B1 (en) | 1999-11-19 | 2004-09-14 | Novartis Ag | Selectable cell surface marker genes |
EP1807510A4 (en) | 2004-09-24 | 2008-01-23 | Angioblast Systems Inc | MEMP (MULTIPOTENTIAL EXPANDED MESENCHYMAL PRECURSOR CELL PROGENY) AND USES THEREOF |
KR20070085288A (ko) | 2004-09-24 | 2007-08-27 | 안지오블라스트 시스템스 인코퍼레이티드 | 간엽 전구세포의 증식 및/또는 생존성 증강 방법 |
ES2654428T3 (es) | 2005-04-12 | 2018-02-13 | Mesoblast, Inc. | Aislamiento de células multipotenciales adultas por fosfatasa alcalina no específica de tejido |
US8609411B2 (en) | 2007-05-04 | 2013-12-17 | Whitehead Institute For Biomedical Research | Ex vivo expansion of human hematopoietic stem cells |
KR20150021587A (ko) | 2007-08-06 | 2015-03-02 | 메소블라스트, 아이엔씨. | 생체 내에서 결합 조직을 생성, 복구 및/또는 유지하는 방법 |
ES2644089T3 (es) * | 2007-12-27 | 2017-11-27 | Baxalta GmbH | Procedimientos de cultivo celular |
KR101713343B1 (ko) | 2008-08-18 | 2017-03-22 | 메소블라스트, 아이엔씨. | 단일 클론 항체 stro-4 |
EP2879684B1 (en) | 2012-08-01 | 2019-01-30 | United Therapeutics Corporation | Treatment of pulmonary arterial hypertension with prostacyclin-treated endothelial progenitor cells |
JP6901823B2 (ja) | 2012-08-01 | 2021-07-14 | ユナイテッド セラピューティクス コーポレイション | 間葉系幹細胞による肺動脈性高血圧症の処置 |
CA2897805C (en) | 2013-01-09 | 2023-03-21 | United Therapeutics Corporation | Treatment of vasculopathy with prostacyclin and mesenchymal stem cells |
JP6599848B2 (ja) | 2013-05-10 | 2019-10-30 | ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ | ソルタギング可能なタンパク質を有する赤血球のinvitro生成 |
FI3851537T3 (fi) * | 2014-04-25 | 2024-03-21 | Genethon | Hyperbilirubinemian hoito |
MY186199A (en) | 2014-11-05 | 2021-06-30 | Juno Therapeutics Inc | Methods for transduction and cell processing |
KR102606101B1 (ko) | 2016-10-24 | 2023-11-27 | 유나이티드 쎄러퓨틱스 코포레이션 | 트레프로스티닐에 의한 msc 면역조절 특성의 향상 |
EP4039786A1 (en) | 2017-10-16 | 2022-08-10 | Regeneron Pharmaceuticals, Inc. | Perfusion bioreactor and related methods of use |
WO2024075819A1 (ja) * | 2022-10-07 | 2024-04-11 | Agc株式会社 | 細胞を用いたウイルスベクターの生産方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1315719C (en) * | 1988-02-05 | 1993-04-06 | Arthur Bank | Retroviral packaging cell lines and processes of using same |
JP3866760B2 (ja) * | 1993-02-22 | 2007-01-10 | ザ ロックフェラー ユニバーシティ | 一過性トランスフェクションによる高力価のヘルパーフリーのレトロウイルスの生産 |
ATE287732T1 (de) * | 1994-08-17 | 2005-02-15 | Genetic Therapy Inc | Retrovirale vektoren, die von gegen lyse durch menschliches serum resistenten produktionszellinien produziert werden |
-
1996
- 1996-12-13 WO PCT/US1996/019904 patent/WO1997021824A2/en not_active Application Discontinuation
- 1996-12-13 AU AU13337/97A patent/AU1333797A/en not_active Abandoned
- 1996-12-13 EP EP96944812A patent/EP0871753A2/en not_active Withdrawn
- 1996-12-13 JP JP9522241A patent/JP2000501614A/ja active Pending
- 1996-12-13 CA CA 2238434 patent/CA2238434A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU1333797A (en) | 1997-07-03 |
EP0871753A2 (en) | 1998-10-21 |
WO1997021824A3 (en) | 1997-09-04 |
JP2000501614A (ja) | 2000-02-15 |
WO1997021824A2 (en) | 1997-06-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |