CA2228176C - Isolation and/or preservation of dendritic cells for prostate cancer immunotherapy - Google Patents
Isolation and/or preservation of dendritic cells for prostate cancer immunotherapy Download PDFInfo
- Publication number
- CA2228176C CA2228176C CA2228176A CA2228176A CA2228176C CA 2228176 C CA2228176 C CA 2228176C CA 2228176 A CA2228176 A CA 2228176A CA 2228176 A CA2228176 A CA 2228176A CA 2228176 C CA2228176 C CA 2228176C
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- CA
- Canada
- Prior art keywords
- leu
- seq
- val
- tyr
- lys
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 206010060862 Prostate cancer Diseases 0.000 title claims abstract description 117
- 208000000236 Prostatic Neoplasms Diseases 0.000 title claims abstract description 115
- 210000004443 dendritic cell Anatomy 0.000 title claims abstract description 81
- 238000002955 isolation Methods 0.000 title description 8
- 238000002619 cancer immunotherapy Methods 0.000 title description 5
- 238000004321 preservation Methods 0.000 title description 3
- 210000001744 T-lymphocyte Anatomy 0.000 claims abstract description 126
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 109
- 239000000427 antigen Substances 0.000 claims abstract description 108
- 108091007433 antigens Proteins 0.000 claims abstract description 106
- 102000036639 antigens Human genes 0.000 claims abstract description 106
- 238000000338 in vitro Methods 0.000 claims abstract description 39
- 201000011510 cancer Diseases 0.000 claims abstract description 28
- 239000000203 mixture Substances 0.000 claims abstract description 22
- 230000004044 response Effects 0.000 claims abstract description 20
- 208000010658 metastatic prostate carcinoma Diseases 0.000 claims abstract description 9
- 210000004027 cell Anatomy 0.000 claims description 126
- 150000001413 amino acids Chemical group 0.000 claims description 109
- 101000892862 Homo sapiens Glutamate carboxypeptidase 2 Proteins 0.000 claims description 46
- 102100041003 Glutamate carboxypeptidase 2 Human genes 0.000 claims description 44
- 239000006166 lysate Substances 0.000 claims description 33
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- 210000002307 prostate Anatomy 0.000 claims description 30
- 206010028980 Neoplasm Diseases 0.000 claims description 29
- 102000007066 Prostate-Specific Antigen Human genes 0.000 claims description 29
- 108010072866 Prostate-Specific Antigen Proteins 0.000 claims description 29
- 210000005259 peripheral blood Anatomy 0.000 claims description 21
- 239000011886 peripheral blood Substances 0.000 claims description 21
- 208000023958 prostate neoplasm Diseases 0.000 claims description 15
- 210000004881 tumor cell Anatomy 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 12
- PDECQIHABNQRHN-GUBZILKMSA-N Asp-Glu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CC(O)=O PDECQIHABNQRHN-GUBZILKMSA-N 0.000 claims description 8
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- DEGCBBCMYWNJNA-RHYQMDGZSA-N Thr-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)[C@@H](C)O DEGCBBCMYWNJNA-RHYQMDGZSA-N 0.000 claims description 8
- KXUKIBHIVRYOIP-ZKWXMUAHSA-N Val-Asp-Cys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)O)N KXUKIBHIVRYOIP-ZKWXMUAHSA-N 0.000 claims description 8
- 238000005138 cryopreservation Methods 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 claims description 7
- QEWBZBLXDKIQPS-STQMWFEESA-N Pro-Gly-Tyr Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O QEWBZBLXDKIQPS-STQMWFEESA-N 0.000 claims description 7
- 230000002401 inhibitory effect Effects 0.000 claims description 7
- 108010054155 lysyllysine Proteins 0.000 claims description 7
- 210000001185 bone marrow Anatomy 0.000 claims description 6
- 108010085325 histidylproline Proteins 0.000 claims description 6
- 230000005907 cancer growth Effects 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 108010044374 isoleucyl-tyrosine Proteins 0.000 claims description 5
- 210000001165 lymph node Anatomy 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 210000000952 spleen Anatomy 0.000 claims description 5
- CNKBMTKICGGSCQ-ACRUOGEOSA-N (2S)-2-[[(2S)-2-[[(2S)-2,6-diamino-1-oxohexyl]amino]-1-oxo-3-phenylpropyl]amino]-3-(4-hydroxyphenyl)propanoic acid Chemical compound C([C@H](NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=CC=C1 CNKBMTKICGGSCQ-ACRUOGEOSA-N 0.000 claims description 4
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- BEMGNWZECGIJOI-WDSKDSINSA-N Ala-Gly-Glu Chemical compound [H]N[C@@H](C)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O BEMGNWZECGIJOI-WDSKDSINSA-N 0.000 claims description 4
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- OYJCVIGKMXUVKB-GARJFASQSA-N Ala-Leu-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N OYJCVIGKMXUVKB-GARJFASQSA-N 0.000 claims description 4
- ZBLQIYPCUWZSRZ-QEJZJMRPSA-N Ala-Phe-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@H](C)N)CC1=CC=CC=C1 ZBLQIYPCUWZSRZ-QEJZJMRPSA-N 0.000 claims description 4
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- ZMUQQMGITUJQTI-CIUDSAMLSA-N Asn-Leu-Asn Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O ZMUQQMGITUJQTI-CIUDSAMLSA-N 0.000 claims description 4
- QGABLMITFKUQDF-DCAQKATOSA-N Asn-Met-Lys Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)N)N QGABLMITFKUQDF-DCAQKATOSA-N 0.000 claims description 4
- JPPLRQVZMZFOSX-UWJYBYFXSA-N Asn-Tyr-Ala Chemical compound NC(=O)C[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](C)C(O)=O)CC1=CC=C(O)C=C1 JPPLRQVZMZFOSX-UWJYBYFXSA-N 0.000 claims description 4
- KQBVNNAPIURMPD-PEFMBERDSA-N Asp-Ile-Glu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(O)=O KQBVNNAPIURMPD-PEFMBERDSA-N 0.000 claims description 4
- HJCGDIGVVWETRO-ZPFDUUQYSA-N Asp-Lys-Ile Chemical compound CC[C@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC(O)=O)C(O)=O HJCGDIGVVWETRO-ZPFDUUQYSA-N 0.000 claims description 4
- UCHSVZYJKJLPHF-BZSNNMDCSA-N Asp-Phe-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O UCHSVZYJKJLPHF-BZSNNMDCSA-N 0.000 claims description 4
- KPSHWSWFPUDEGF-FXQIFTODSA-N Asp-Pro-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CC(O)=O KPSHWSWFPUDEGF-FXQIFTODSA-N 0.000 claims description 4
- QSFHZPQUAAQHAQ-CIUDSAMLSA-N Asp-Ser-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O QSFHZPQUAAQHAQ-CIUDSAMLSA-N 0.000 claims description 4
- SQIARYGNVQWOSB-BZSNNMDCSA-N Asp-Tyr-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O SQIARYGNVQWOSB-BZSNNMDCSA-N 0.000 claims description 4
- MUZAUPFGPMMZSS-GUBZILKMSA-N Cys-Glu-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CS)N MUZAUPFGPMMZSS-GUBZILKMSA-N 0.000 claims description 4
- KXHAPEPORGOXDT-UWJYBYFXSA-N Cys-Tyr-Ala Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C)C(O)=O KXHAPEPORGOXDT-UWJYBYFXSA-N 0.000 claims description 4
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- VPKBCVUDBNINAH-GARJFASQSA-N Glu-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCC(=O)O)N)C(=O)O VPKBCVUDBNINAH-GARJFASQSA-N 0.000 claims description 4
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- XVYKMNXXJXQKME-XEGUGMAKSA-N Gly-Ile-Tyr Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 XVYKMNXXJXQKME-XEGUGMAKSA-N 0.000 claims description 4
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- VNNRLUNBJSWZPF-ZKWXMUAHSA-N Gly-Ser-Ile Chemical compound [H]NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VNNRLUNBJSWZPF-ZKWXMUAHSA-N 0.000 claims description 4
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/509,254 US5788963A (en) | 1995-07-31 | 1995-07-31 | Isolation and/or preservation of dendritic cells for prostate cancer immunotherapy |
| US509,254 | 1995-07-31 | ||
| PCT/US1996/012389 WO1997004802A1 (en) | 1995-07-31 | 1996-07-29 | Isolation and/or preservation of dendritic cells for prostate cancer immunotherapy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2228176A1 CA2228176A1 (en) | 1997-02-13 |
| CA2228176C true CA2228176C (en) | 2015-05-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2228176A Expired - Lifetime CA2228176C (en) | 1995-07-31 | 1996-07-29 | Isolation and/or preservation of dendritic cells for prostate cancer immunotherapy |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US5788963A (enExample) |
| EP (2) | EP2377548A3 (enExample) |
| JP (1) | JP4267692B2 (enExample) |
| AU (1) | AU723607B2 (enExample) |
| CA (1) | CA2228176C (enExample) |
| DE (1) | DE841940T1 (enExample) |
| IL (1) | IL123079A0 (enExample) |
| NZ (1) | NZ315441A (enExample) |
| WO (1) | WO1997004802A1 (enExample) |
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| US3753357A (en) | 1970-12-14 | 1973-08-21 | Ovitron Res Corp | Method and apparatus for the preservation of cells and tissues |
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| CA2192655A1 (en) * | 1994-06-14 | 1995-12-21 | Edgar G. Engleman | Methods for in vivo t cell activation by antigen-pulsed dendritic cells |
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1995
- 1995-07-31 US US08/509,254 patent/US5788963A/en not_active Expired - Lifetime
-
1996
- 1996-07-29 CA CA2228176A patent/CA2228176C/en not_active Expired - Lifetime
- 1996-07-29 EP EP10183831A patent/EP2377548A3/en not_active Withdrawn
- 1996-07-29 DE DE0841940T patent/DE841940T1/de active Pending
- 1996-07-29 EP EP96927268A patent/EP0841940A4/en not_active Withdrawn
- 1996-07-29 AU AU67146/96A patent/AU723607B2/en not_active Expired
- 1996-07-29 NZ NZ315441A patent/NZ315441A/xx unknown
- 1996-07-29 WO PCT/US1996/012389 patent/WO1997004802A1/en not_active Ceased
- 1996-07-29 IL IL12307996A patent/IL123079A0/xx not_active IP Right Cessation
- 1996-07-29 JP JP50782397A patent/JP4267692B2/ja not_active Expired - Lifetime
-
2012
- 2012-06-14 US US13/523,828 patent/US20130017600A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001520633A (ja) | 2001-10-30 |
| DE841940T1 (de) | 2001-08-23 |
| EP2377548A2 (en) | 2011-10-19 |
| CA2228176A1 (en) | 1997-02-13 |
| US5788963A (en) | 1998-08-04 |
| AU6714696A (en) | 1997-02-26 |
| JP4267692B2 (ja) | 2009-05-27 |
| EP2377548A3 (en) | 2012-10-10 |
| MX9800821A (es) | 1998-10-31 |
| NZ315441A (en) | 1999-06-29 |
| AU723607B2 (en) | 2000-08-31 |
| US20130017600A1 (en) | 2013-01-17 |
| WO1997004802A1 (en) | 1997-02-13 |
| IL123079A0 (en) | 1998-09-24 |
| EP0841940A1 (en) | 1998-05-20 |
| EP0841940A4 (en) | 2005-01-05 |
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