CA2218188A1 - Sequencage de biopolymeres en phase solide - Google Patents

Sequencage de biopolymeres en phase solide Download PDF

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Publication number
CA2218188A1
CA2218188A1 CA 2218188 CA2218188A CA2218188A1 CA 2218188 A1 CA2218188 A1 CA 2218188A1 CA 2218188 CA2218188 CA 2218188 CA 2218188 A CA2218188 A CA 2218188A CA 2218188 A1 CA2218188 A1 CA 2218188A1
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CA
Canada
Prior art keywords
sequence
nucleic acid
target
mass
strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2218188
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English (en)
Inventor
Charles R. Cantor
Hubert Koster
Cassandra L. Smith
Dong-Jing Fu
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Boston University
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Individual
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Filing date
Publication date
Priority claimed from US08/614,151 external-priority patent/US6436635B1/en
Application filed by Individual filed Critical Individual
Publication of CA2218188A1 publication Critical patent/CA2218188A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention porte sur des procédés de détection et de séquençage de séquences d'acides nucléiques cibles et de séquences d'acides nucléiques double brin, sur des sondes d'acide nucléique, sur des sondes d'acide nucléique modifiées en masse, sur des groupes de sondes intervenant dans des procédés, et sur des trousses et systèmes contenant lesdites sondes. Les procédés efficaces consistent à hybrider les acides nucléiques ou ceux qui représentent des séquences complémentaires ou homologues de la cible à un groupe de sondes d'acide nucléique lesquelles comportent une portion monobrin, une portion facultative double-brin et une séquence variable intérieure à la portion monobrin. Les poids moléculaires des acides nucléiques hybridés de l'ensemble peuvent être déterminés par spectrométrie de masse et la séquence de la cible, peut l'être en fonction du poids moléculaire des fragments. Les acides nucléiques dont les séquences peuvent être déterminées comportent des ADN et des ARN sous forme d'échantillons biologiques, tels que des biopsies prélevées sur des patients ou des échantillons prélevés dans l'environnement. Les sondes peuvent être fixées à un support solide du type pastille d'hybridation pour faciliter la mesure des poids moléculaires et l'identification de la séquence de la cible.
CA 2218188 1995-04-11 1996-04-10 Sequencage de biopolymeres en phase solide Abandoned CA2218188A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US42000995A 1995-04-11 1995-04-11
US41999495A 1995-04-11 1995-04-11
US08/419,994 1995-04-11
US08/420,009 1995-04-11
US08/614,151 US6436635B1 (en) 1992-11-06 1996-03-12 Solid phase sequencing of double-stranded nucleic acids
US08/614,151 1996-03-12

Publications (1)

Publication Number Publication Date
CA2218188A1 true CA2218188A1 (fr) 1996-10-17

Family

ID=27411260

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2218188 Abandoned CA2218188A1 (fr) 1995-04-11 1996-04-10 Sequencage de biopolymeres en phase solide

Country Status (5)

Country Link
EP (1) EP0830460A1 (fr)
JP (1) JPH11503611A (fr)
AU (1) AU5544696A (fr)
CA (1) CA2218188A1 (fr)
WO (1) WO1996032504A2 (fr)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547835A (en) 1993-01-07 1996-08-20 Sequenom, Inc. DNA sequencing by mass spectrometry
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6428955B1 (en) * 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US6146854A (en) * 1995-08-31 2000-11-14 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
DE19543065C2 (de) * 1995-11-09 2002-10-31 Gag Bioscience Gmbh Zentrum Fu Verfahren zur Genomanalyse und Mittel zur Durchführung des Verfahrens
EP0886681A1 (fr) * 1996-03-04 1998-12-30 Genetrace Systems, Inc. Methodes de criblage des acides nucleiques par spectrometrie de masse
US5777324A (en) * 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis
GB9622665D0 (en) * 1996-10-31 1997-01-08 Zeneca Ltd Methods
US20030129589A1 (en) * 1996-11-06 2003-07-10 Hubert Koster Dna diagnostics based on mass spectrometry
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6699668B1 (en) 1997-01-15 2004-03-02 Xzillion Gmbh & Co. Mass label linked hybridisation probes
AU725966B2 (en) * 1997-01-15 2000-10-26 Xzillion Gmbh & Co. Kg Mass label linked hybridisation probes
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
WO1998040520A1 (fr) * 1997-03-14 1998-09-17 Hybridon, Inc. Methode de sequençage d'acides nucleiques modifies au moyen d'une spectrometrie de masse a transformee de fourier a ionisation par electropulverisation
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
JP2001517948A (ja) * 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
US7056659B1 (en) 1997-07-11 2006-06-06 Xzillion Gmbh & Co. Characterizing nucleic acids
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6268131B1 (en) * 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
FR2782325B1 (fr) * 1998-08-12 2002-05-24 Proteus Procede d'identification de sequences polynucleotidiques et/ou des proteines correspondantes a partir d'un echantillon d'acides nucleiques
US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6104028A (en) * 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
US7270958B2 (en) 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
DE19963536C2 (de) * 1999-12-20 2003-04-10 Epigenomics Ag Verfahren zur Analyse von Nukleinsäuresequenzen
JP2001204463A (ja) * 2000-01-27 2001-07-31 Toyo Kohan Co Ltd ヌクレオチド固定用担体
JP4015946B2 (ja) 2000-10-30 2007-11-28 シークエノム・インコーポレーテツド 基板上にサブマイクロリットルの体積を供給する方法及び装置
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20040121335A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents associated with host versus graft and graft versus host rejections
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
JP2003043037A (ja) * 2001-07-27 2003-02-13 Inst Of Physical & Chemical Res ハイブリダイゼーション用基板、この基板の製造方法及び使用方法
GB0202462D0 (en) * 2002-02-04 2002-03-20 Tepnel Medical Ltd Nucleic acid analysis
EP1578399A4 (fr) 2002-12-06 2007-11-28 Isis Pharmaceuticals Inc Procedes d'identification rapide de pathogenes chez l'homme et les betes
US20060051879A9 (en) 2003-01-16 2006-03-09 Hubert Koster Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US8288523B2 (en) 2003-09-11 2012-10-16 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
WO2005117270A2 (fr) 2004-05-24 2005-12-08 Isis Pharmaceuticals, Inc. Spectrometrie de masse a filtration ionique selective par seuillage numerique
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (fr) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Procedes pour l'identification rapide d'organismes recombinants
GB0500996D0 (en) * 2005-01-18 2005-02-23 Delfts Diagnostic Labaratory B Detection method and materials therefor
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
EP1869180B1 (fr) 2005-03-03 2013-02-20 Ibis Biosciences, Inc. Compositions utilisées pour identifier des virus polyoma
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
US9550162B2 (en) * 2005-09-19 2017-01-24 Intematix Corporation Printing liquid solution arrays for inorganic combinatorial libraries
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
CA2641851A1 (fr) 2006-02-08 2007-08-16 Eric Hans Vermaas Procede de sequencage d'une matrice polynucleotidique
US8088582B2 (en) 2006-04-06 2012-01-03 Ibis Biosciences, Inc. Compositions for the use in identification of fungi
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2121983A2 (fr) 2007-02-02 2009-11-25 Illumina Cambridge Limited Procedes pour indexer des echantillons et sequencer de multiples matrices nucleotidiques
WO2008104002A2 (fr) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Procédé d'analyse d'adn médico-légale rapide
WO2008151023A2 (fr) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Procédés et compositions pour l'amplification par déplacement multiple d'acides nucléiques
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US20090180931A1 (en) 2007-09-17 2009-07-16 Sequenom, Inc. Integrated robotic sample transfer device
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
WO2010033627A2 (fr) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Unités de traitement d'échantillons, systèmes et procédés associés
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
WO2010093943A1 (fr) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ensembles sonde d'ionisation
US9719083B2 (en) 2009-03-08 2017-08-01 Ibis Biosciences, Inc. Bioagent detection methods
WO2010114842A1 (fr) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Systèmes, dispositifs et procédés de détection d'agent biologique
WO2011008971A1 (fr) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Appareil de levage et de montage
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
US9080209B2 (en) 2009-08-06 2015-07-14 Ibis Biosciences, Inc. Non-mass determined base compositions for nucleic acid detection
SI2669387T1 (sl) 2009-08-25 2017-01-31 Illumina, Inc. Metode za izbiro in pomnoževanje polinukleotidov
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
EP2488656B1 (fr) 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Amplification par déplacement multiple
WO2011115840A2 (fr) 2010-03-14 2011-09-22 Ibis Biosciences, Inc. Recherche de parasites par le biais de la recherche d'endosymbiotes
EA027558B1 (ru) 2011-05-19 2017-08-31 Эйджена Байосайенс, Инк. Способ обнаружения мультиплексных нуклеиновых кислот
US8829426B2 (en) 2011-07-14 2014-09-09 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry
CN105705657B (zh) * 2013-10-18 2020-07-28 Seegene株式会社 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测
WO2016172579A1 (fr) 2015-04-24 2016-10-27 Agena Bioscience, Inc, Procédés multiplexes permettant la détection et la quantification de variants mineurs
CN114540470A (zh) 2015-04-24 2022-05-27 基纳生物技术有限公司 用于次要等位基因和多态性的鉴定和定量的多重化方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5547835A (en) * 1993-01-07 1996-08-20 Sequenom, Inc. DNA sequencing by mass spectrometry
US5650274A (en) * 1993-06-25 1997-07-22 Hitachi, Ltd. DNA analyzing method

Also Published As

Publication number Publication date
EP0830460A1 (fr) 1998-03-25
WO1996032504A3 (fr) 1996-11-14
JPH11503611A (ja) 1999-03-30
WO1996032504A2 (fr) 1996-10-17
AU5544696A (en) 1996-10-30

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