CA2218188A1 - Sequencage de biopolymeres en phase solide - Google Patents

Sequencage de biopolymeres en phase solide Download PDF

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Publication number
CA2218188A1
CA2218188A1 CA 2218188 CA2218188A CA2218188A1 CA 2218188 A1 CA2218188 A1 CA 2218188A1 CA 2218188 CA2218188 CA 2218188 CA 2218188 A CA2218188 A CA 2218188A CA 2218188 A1 CA2218188 A1 CA 2218188A1
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CA
Canada
Prior art keywords
sequence
nucleic acid
target
mass
strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2218188
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English (en)
Inventor
Charles R. Cantor
Hubert Koster
Cassandra L. Smith
Dong-Jing Fu
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Boston University
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Individual
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Filing date
Publication date
Priority claimed from US08/614,151 external-priority patent/US6436635B1/en
Application filed by Individual filed Critical Individual
Publication of CA2218188A1 publication Critical patent/CA2218188A1/fr
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6872Methods for sequencing involving mass spectrometry
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention porte sur des procédés de détection et de séquençage de séquences d'acides nucléiques cibles et de séquences d'acides nucléiques double brin, sur des sondes d'acide nucléique, sur des sondes d'acide nucléique modifiées en masse, sur des groupes de sondes intervenant dans des procédés, et sur des trousses et systèmes contenant lesdites sondes. Les procédés efficaces consistent à hybrider les acides nucléiques ou ceux qui représentent des séquences complémentaires ou homologues de la cible à un groupe de sondes d'acide nucléique lesquelles comportent une portion monobrin, une portion facultative double-brin et une séquence variable intérieure à la portion monobrin. Les poids moléculaires des acides nucléiques hybridés de l'ensemble peuvent être déterminés par spectrométrie de masse et la séquence de la cible, peut l'être en fonction du poids moléculaire des fragments. Les acides nucléiques dont les séquences peuvent être déterminées comportent des ADN et des ARN sous forme d'échantillons biologiques, tels que des biopsies prélevées sur des patients ou des échantillons prélevés dans l'environnement. Les sondes peuvent être fixées à un support solide du type pastille d'hybridation pour faciliter la mesure des poids moléculaires et l'identification de la séquence de la cible.
CA 2218188 1995-04-11 1996-04-10 Sequencage de biopolymeres en phase solide Abandoned CA2218188A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US42000995A 1995-04-11 1995-04-11
US41999495A 1995-04-11 1995-04-11
US08/419,994 1995-04-11
US08/420,009 1995-04-11
US08/614,151 US6436635B1 (en) 1992-11-06 1996-03-12 Solid phase sequencing of double-stranded nucleic acids
US08/614,151 1996-03-12

Publications (1)

Publication Number Publication Date
CA2218188A1 true CA2218188A1 (fr) 1996-10-17

Family

ID=27411260

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2218188 Abandoned CA2218188A1 (fr) 1995-04-11 1996-04-10 Sequencage de biopolymeres en phase solide

Country Status (5)

Country Link
EP (1) EP0830460A1 (fr)
JP (1) JPH11503611A (fr)
AU (1) AU5544696A (fr)
CA (1) CA2218188A1 (fr)
WO (1) WO1996032504A2 (fr)

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Also Published As

Publication number Publication date
EP0830460A1 (fr) 1998-03-25
WO1996032504A3 (fr) 1996-11-14
JPH11503611A (ja) 1999-03-30
WO1996032504A2 (fr) 1996-10-17
AU5544696A (en) 1996-10-30

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