CA2197668A1 - Chemiluminescent detection of hydrolytic enzymes using an acridan - Google Patents
Chemiluminescent detection of hydrolytic enzymes using an acridanInfo
- Publication number
- CA2197668A1 CA2197668A1 CA002197668A CA2197668A CA2197668A1 CA 2197668 A1 CA2197668 A1 CA 2197668A1 CA 002197668 A CA002197668 A CA 002197668A CA 2197668 A CA2197668 A CA 2197668A CA 2197668 A1 CA2197668 A1 CA 2197668A1
- Authority
- CA
- Canada
- Prior art keywords
- group
- compound
- substituted
- acridan
- hydrolytic enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
- C07D219/02—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with only hydrogen, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the ring system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
- C07D219/04—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the ring system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
- C07D219/04—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the ring system
- C07D219/06—Oxygen atoms
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B15/00—Acridine dyes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/300,367 US5686258A (en) | 1993-05-17 | 1994-09-02 | Chemiluminescent detection of hydrolytic enzymes using an acridan |
US08/300,367 | 1994-09-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2197668A1 true CA2197668A1 (en) | 1996-03-14 |
Family
ID=23158797
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002197668A Abandoned CA2197668A1 (en) | 1994-09-02 | 1995-08-30 | Chemiluminescent detection of hydrolytic enzymes using an acridan |
Country Status (8)
Country | Link |
---|---|
US (1) | US5686258A (ja) |
EP (1) | EP0778947B1 (ja) |
JP (1) | JP3828150B2 (ja) |
AT (1) | ATE266867T1 (ja) |
AU (1) | AU707682B2 (ja) |
CA (1) | CA2197668A1 (ja) |
DE (1) | DE69533035T2 (ja) |
WO (1) | WO1996007911A1 (ja) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5843666A (en) * | 1994-09-02 | 1998-12-01 | Lumigen, Inc. | Chemiluminescent detection methods using dual enzyer-labeled binding partners |
US6410732B2 (en) * | 1996-01-16 | 2002-06-25 | Lumigen, Inc. | Processing and synthetic intermediates for preparing N-arylacridancarboxylic acid derivatives |
CN1173954C (zh) * | 1996-01-16 | 2004-11-03 | 鲁米根公司 | 与磷酸酶反应产生化学发光的化合物、组合物和方法 |
US5772926A (en) * | 1997-05-13 | 1998-06-30 | Lumigen, Inc. | Chemiluminescent reactions using dihydroxyaromatic compounds and heterocyclic enol phosphates |
US6068979A (en) * | 1998-04-22 | 2000-05-30 | Lumigen, Inc. | Simplified sequential chemiluminescent detection |
US6017769A (en) * | 1998-06-17 | 2000-01-25 | Lumigen, Inc. | Non-enzymatic methods of generating chemiluminescence from acridan alkenes |
US6783948B1 (en) | 1999-07-30 | 2004-08-31 | Bayer Corporation | Chemiluminescent acridinium compounds and analogues thereof as substrates of hydrolytic enzymes |
JP4733334B2 (ja) * | 2000-06-26 | 2011-07-27 | ルミゲン インコーポレイテッド | アクリダン化合物からの電気化学ルミネセンス |
US7416680B2 (en) * | 2001-10-12 | 2008-08-26 | International Business Machines Corporation | Self-cleaning colloidal slurry composition and process for finishing a surface of a substrate |
WO2006116683A1 (en) * | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanostructure enhanced luminescence |
WO2006116687A2 (en) * | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanoassays |
WO2006116686A2 (en) * | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanostructure enhanced luminescent devices |
ITBO20070112A1 (it) * | 2007-02-23 | 2008-08-24 | Cyanagen Srl | Metodo per incrementare l'emissione di luce da una reazione chemiluminescente |
JP5414424B2 (ja) * | 2009-08-31 | 2014-02-12 | シスメックス株式会社 | 目的物質の検出方法、及び目的物質を検出するためのクロマトグラフィー用試験キット |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4918192A (en) * | 1986-10-06 | 1990-04-17 | Ciba Corning Diagnostics Corp. | Polysubstituted aryl acridinium esters |
US5284952A (en) * | 1987-12-31 | 1994-02-08 | London Diagnostics, Inc. | Sulfonyl substituted chemiluminescent labels and their conjugates, and assays therefrom |
DE68911633T2 (de) * | 1988-09-30 | 1994-04-07 | Fujirebio Kk | Verfahren zur Bestimmung von Peroxidase-Aktivität unter Verwendung von Chemilumineszenz. |
JPH0786096B1 (ja) * | 1989-04-28 | 1995-09-20 | ||
US5306621A (en) * | 1989-10-17 | 1994-04-26 | British Technology Group Limited | Enhanced chemiluminescent assay |
US5491072A (en) * | 1993-05-17 | 1996-02-13 | Lumigen, Inc. | N-alkylacridan carboxyl derivatives useful for chemiluminescent detection |
-
1994
- 1994-09-02 US US08/300,367 patent/US5686258A/en not_active Expired - Lifetime
-
1995
- 1995-08-30 WO PCT/US1995/010952 patent/WO1996007911A1/en active IP Right Grant
- 1995-08-30 JP JP50955096A patent/JP3828150B2/ja not_active Expired - Lifetime
- 1995-08-30 AU AU35411/95A patent/AU707682B2/en not_active Ceased
- 1995-08-30 CA CA002197668A patent/CA2197668A1/en not_active Abandoned
- 1995-08-30 DE DE69533035T patent/DE69533035T2/de not_active Expired - Lifetime
- 1995-08-30 AT AT95932341T patent/ATE266867T1/de not_active IP Right Cessation
- 1995-08-30 EP EP95932341A patent/EP0778947B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5686258A (en) | 1997-11-11 |
EP0778947A1 (en) | 1997-06-18 |
AU3541195A (en) | 1996-03-27 |
AU707682B2 (en) | 1999-07-15 |
JP3828150B2 (ja) | 2006-10-04 |
ATE266867T1 (de) | 2004-05-15 |
EP0778947B1 (en) | 2004-05-12 |
JPH10505495A (ja) | 1998-06-02 |
EP0778947A4 (en) | 1999-01-27 |
DE69533035D1 (de) | 2004-06-17 |
WO1996007911A1 (en) | 1996-03-14 |
DE69533035T2 (de) | 2005-04-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |