CA2195202A1 - Separation de l'albumine serique humaine - Google Patents

Separation de l'albumine serique humaine

Info

Publication number
CA2195202A1
CA2195202A1 CA 2195202 CA2195202A CA2195202A1 CA 2195202 A1 CA2195202 A1 CA 2195202A1 CA 2195202 CA2195202 CA 2195202 CA 2195202 A CA2195202 A CA 2195202A CA 2195202 A1 CA2195202 A1 CA 2195202A1
Authority
CA
Canada
Prior art keywords
hsa
bsa
serum albumin
ligand
blue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2195202
Other languages
English (en)
Inventor
Jan H. Nuijens
Emile J. J. M. Van Corven
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharming BV
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2195202A1 publication Critical patent/CA2195202A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Du lait provenant de bovins transgéniques contient de l'albumine sérique humaine (hSA) et une albumine sérique bovine endogène (bSA). On purifie la hSA jusqu'à une pureté d'au moins 98 % par un procédé qui inclut une chromatographie d'affinité en présence d'un détergent, le ligand d'affinité étant des molécules d'un colorant, la triazine. L'étape de chromatographique d'affinité peut être incorporée dans une protocole visant à fournir une hSA recombinée pure par SDS-PAGE et des radio-immunodosages.
CA 2195202 1994-07-20 1995-07-20 Separation de l'albumine serique humaine Abandoned CA2195202A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9414651.1 1994-07-20
GB9414651A GB9414651D0 (en) 1994-07-20 1994-07-20 Separation of human serum albumin

Publications (1)

Publication Number Publication Date
CA2195202A1 true CA2195202A1 (fr) 1996-02-01

Family

ID=10758626

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2195202 Abandoned CA2195202A1 (fr) 1994-07-20 1995-07-20 Separation de l'albumine serique humaine

Country Status (6)

Country Link
EP (1) EP0773961A1 (fr)
JP (1) JPH10504289A (fr)
AU (1) AU2989495A (fr)
CA (1) CA2195202A1 (fr)
GB (1) GB9414651D0 (fr)
WO (1) WO1996002573A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001527090A (ja) * 1997-12-31 2001-12-25 アメルシャム・ファルマシア・バイオテック・アクチボラグ アルブミンを結合する方法およびその方法で使用すべき手段
SE9802213D0 (sv) * 1998-06-18 1998-06-18 Amersham Pharm Biotech Ab A method for the removal/purification of serum albumins and means for use in the method
SE9804465D0 (sv) * 1998-12-22 1998-12-22 Amersham Pharm Biotech Ab A method for the removal/purification of serum albumins and means for use in the method
DE10026998A1 (de) * 2000-05-31 2001-12-13 Fresenius Kabi De Gmbh Verfahren zur Herstellung einer kosmetischen Zusammensetzung, die humanes Serum Albumin umfasst, welches aus transgenen nicht-menschlichen Säugern erhalten wurde
JP4706093B2 (ja) * 2000-09-25 2011-06-22 富士レビオ株式会社 パルボウイルスの除去方法及び精製方法
US20030036637A1 (en) * 2001-06-13 2003-02-20 Scott Fulton Purification of human serum albumin
US20040217055A1 (en) * 2003-03-12 2004-11-04 Elmar Kraus Use of recombinant albumin in dialysis after liver failure
JP4511847B2 (ja) * 2004-02-16 2010-07-28 積水化学工業株式会社 ヘモグロビンＡ１ｃの測定方法
CA3014789C (fr) * 2007-06-08 2019-11-05 Quest Diagnostics Investments Incorporated Analyse de lipoproteines par mobilite differentielle de particules chargees
CN101367864B (zh) * 2008-08-27 2011-12-14 中国科学院过程工程研究所 异源同种乳清蛋白的分离纯化方法
CN101948529B (zh) * 2010-08-13 2013-05-01 中国科学院过程工程研究所 一种高效纯化金属离子结合蛋白的疏水层析分离纯化方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2090299T3 (es) * 1989-12-01 1996-10-16 Pharming Bv Produccion de polipeptidos recombinantes mediante especies bovinas y metodos transgenicos.
EP0546127B1 (fr) * 1991-03-07 1996-09-04 Dade International Inc. Echantillon controle biosynthetique de fluide cerebrospinal et procede de fabrication de cet echantillon

Also Published As

Publication number Publication date
GB9414651D0 (en) 1994-09-07
JPH10504289A (ja) 1998-04-28
AU2989495A (en) 1996-02-16
WO1996002573A1 (fr) 1996-02-01
EP0773961A1 (fr) 1997-05-21

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Legal Events

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