CA2182194A1 - Methode diagnostique de l'acide nucleique recourant aux ribosondes, a la digestion de la rnase et a la chromatographie par exclusion de poids moleculaire - Google Patents

Methode diagnostique de l'acide nucleique recourant aux ribosondes, a la digestion de la rnase et a la chromatographie par exclusion de poids moleculaire

Info

Publication number
CA2182194A1
CA2182194A1 CA002182194A CA2182194A CA2182194A1 CA 2182194 A1 CA2182194 A1 CA 2182194A1 CA 002182194 A CA002182194 A CA 002182194A CA 2182194 A CA2182194 A CA 2182194A CA 2182194 A1 CA2182194 A1 CA 2182194A1
Authority
CA
Canada
Prior art keywords
nucleic acid
probe
target
dna
hpv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002182194A
Other languages
English (en)
Inventor
Francis H. Martin
Frederick W. Jacobsen
Calvert L. Green
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2182194A1 publication Critical patent/CA2182194A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Méthode améliorée de détection de séquences particulières d'acide nucléique par hybridation de l'acide nucléique et méthode de diagnostic où des ribosondes détectables par marquage sont hybridées en solution à des séquences complémentaires d'acide nucléique codées par des molécules cibles d'acide nucléique. Après l'hybridation, la digestion de la RNAse sert à dégrader les molécules sondes non hybridées après quoi on recourt à la séparation chromatographique pour isoler les hybrides de sondes-cibles. Cette méthode améliore les limites de détection de l'hybridation de l'acide nucléique et présente une utilité générale en matière de diagnostic médical, de chimie légale et de recherche en biologie moléculaire.
CA002182194A 1993-10-22 1994-10-19 Methode diagnostique de l'acide nucleique recourant aux ribosondes, a la digestion de la rnase et a la chromatographie par exclusion de poids moleculaire Abandoned CA2182194A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14171193A 1993-10-22 1993-10-22
US08/141,711 1993-10-22

Publications (1)

Publication Number Publication Date
CA2182194A1 true CA2182194A1 (fr) 1995-04-27

Family

ID=22496882

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002182194A Abandoned CA2182194A1 (fr) 1993-10-22 1994-10-19 Methode diagnostique de l'acide nucleique recourant aux ribosondes, a la digestion de la rnase et a la chromatographie par exclusion de poids moleculaire

Country Status (6)

Country Link
EP (1) EP0738331A1 (fr)
AU (1) AU8085394A (fr)
CA (1) CA2182194A1 (fr)
HU (1) HUT75069A (fr)
NZ (1) NZ275286A (fr)
WO (1) WO1995011316A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2737502B1 (fr) * 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
WO2006105487A1 (fr) * 2005-03-31 2006-10-05 Amgen Inc. Procede de blocage selectif de l'amplification de l'arn dans l'hemoglobine
EP3124596A1 (fr) * 2009-09-16 2017-02-01 Life Technologies Corporation Tampons de lyse pour extraire des acides nucléiques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229442B1 (fr) * 1986-01-07 1993-01-27 Gen-Probe Incorporated Procédé de réassociation accélérée d'acide nucléique
WO1992018649A1 (fr) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions et procedes d'extraction et d'hybridation ameliorees d'acide nucleique

Also Published As

Publication number Publication date
WO1995011316A1 (fr) 1995-04-27
NZ275286A (en) 1998-08-26
HU9602069D0 (en) 1996-09-30
HUT75069A (en) 1997-04-28
EP0738331A1 (fr) 1996-10-23
AU8085394A (en) 1995-05-08

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued