CA2178590A1 - Reactifs et procedes de detection du methotrexate - Google Patents
Reactifs et procedes de detection du methotrexateInfo
- Publication number
- CA2178590A1 CA2178590A1 CA 2178590 CA2178590A CA2178590A1 CA 2178590 A1 CA2178590 A1 CA 2178590A1 CA 2178590 CA2178590 CA 2178590 CA 2178590 A CA2178590 A CA 2178590A CA 2178590 A1 CA2178590 A1 CA 2178590A1
- Authority
- CA
- Canada
- Prior art keywords
- antibody
- methotrexate
- cell line
- test sample
- hydroxymethotrexate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention concerne un anticorps monoclonal murin IgG utilisé dans la détection du méthotrexate dans les liquides biologiques. Cet anticorps monoclonal désigné par 4-354-178 peut être utilisé dans une technique de détection du méthotrexate, de préférence un dosage immunologique à polarisation de fluorescence (FPIA) de l'urine. L'anticorps monoclonal 4-351-178 se caractérise par l'absence de réactivité croisée détectable avec le 7-hydroxyméthotrexate. L'invention porte également sur une lignée cellulaire d'hybridome, ATC HB 11467, produisant l'anticorps monoclonal selon l'invention.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16512193A | 1993-12-10 | 1993-12-10 | |
US08/165,121 | 1993-12-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2178590A1 true CA2178590A1 (fr) | 1995-06-15 |
Family
ID=22597511
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2178590 Abandoned CA2178590A1 (fr) | 1993-12-10 | 1994-11-22 | Reactifs et procedes de detection du methotrexate |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0733102A1 (fr) |
JP (1) | JPH09506260A (fr) |
AU (1) | AU1186695A (fr) |
CA (1) | CA2178590A1 (fr) |
WO (1) | WO1995016026A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107219357A (zh) * | 2017-04-21 | 2017-09-29 | 中国人民解放军第三军医大学第附属医院 | 一种检测甲氨蝶呤的elisa试剂盒及其应用 |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5710009A (en) * | 1994-02-17 | 1998-01-20 | Serex, Inc. | Receptor:release ligand (reland) complexes and release assays using said reland and methods and kits based thereon |
AU2678997A (en) * | 1996-04-19 | 1997-11-12 | Lexigen Pharmaceuticals Corp. | Inhibition of the binding of human ige to its receptor by tetracyclic compounds for the alleviation of ige-mediated immune response |
MX2010013239A (es) | 2008-06-03 | 2011-02-24 | Abbott Lab | Inmunoglobulinas de dominio variable doble y usos de las mismas. |
UY32870A (es) * | 2009-09-01 | 2011-02-28 | Abbott Lab | Inmunoglobulinas con dominio variable dual y usos de las mismas |
TW201206473A (en) | 2010-08-03 | 2012-02-16 | Abbott Lab | Dual variable domain immunoglobulins and uses thereof |
JP2013539364A (ja) | 2010-08-26 | 2013-10-24 | アッヴィ・インコーポレイテッド | 二重可変ドメイン免疫グロブリンおよびその使用 |
MX2014008101A (es) | 2011-12-30 | 2014-09-25 | Abbvie Inc | Proteinas de union especificas duales dirigidas contra il-13 y/o il-17. |
TW201811825A (zh) | 2012-11-01 | 2018-04-01 | 美商艾伯維有限公司 | 抗-vegf/dll4雙重可變區域免疫球蛋白及其用途 |
TW201512219A (zh) | 2013-03-15 | 2015-04-01 | Abbvie Inc | 針對IL-1β及/或IL-17之雙特異性結合蛋白 |
WO2016094881A2 (fr) | 2014-12-11 | 2016-06-16 | Abbvie Inc. | Protéines de liaison à lrp-8 |
CN104569373B (zh) * | 2015-01-27 | 2016-08-17 | 苏州博源医疗科技有限公司 | 一种甲氨蝶呤均相酶免疫检测试剂及其制备和检测方法 |
TW201710286A (zh) | 2015-06-15 | 2017-03-16 | 艾伯維有限公司 | 抗vegf、pdgf及/或其受體之結合蛋白 |
WO2016210054A1 (fr) * | 2015-06-23 | 2016-12-29 | The Board Of Trustees Of The Leland Stanford Junior University | Nouveaux fluorophores de rhodol destinés à une imagerie dans le proche infrarouge |
WO2017007889A1 (fr) * | 2015-07-08 | 2017-01-12 | Defined Diagnostics, Llc | Composés et méthodes pour la détection du méthotrexate |
CN108614107A (zh) * | 2016-12-12 | 2018-10-02 | 上海复星长征医学科学有限公司 | 一种甲氨蝶呤胶乳免疫比浊测定试剂盒及其制备方法 |
CN110357886B (zh) * | 2018-04-09 | 2022-06-24 | 浙江准策生物技术有限公司 | 甲氨蝶呤半抗原和完全抗原及其制备方法和应用 |
-
1994
- 1994-11-22 WO PCT/US1994/013558 patent/WO1995016026A1/fr not_active Application Discontinuation
- 1994-11-22 CA CA 2178590 patent/CA2178590A1/fr not_active Abandoned
- 1994-11-22 JP JP7516209A patent/JPH09506260A/ja not_active Ceased
- 1994-11-22 EP EP95902688A patent/EP0733102A1/fr not_active Withdrawn
- 1994-11-22 AU AU11866/95A patent/AU1186695A/en not_active Abandoned
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107219357A (zh) * | 2017-04-21 | 2017-09-29 | 中国人民解放军第三军医大学第附属医院 | 一种检测甲氨蝶呤的elisa试剂盒及其应用 |
Also Published As
Publication number | Publication date |
---|---|
WO1995016026A1 (fr) | 1995-06-15 |
EP0733102A1 (fr) | 1996-09-25 |
AU1186695A (en) | 1995-06-27 |
JPH09506260A (ja) | 1997-06-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Dead |