CA2161973A1 - Yeast strain and methods for expressing heterologous proteins in yeast - Google Patents

Yeast strain and methods for expressing heterologous proteins in yeast

Info

Publication number
CA2161973A1
CA2161973A1 CA002161973A CA2161973A CA2161973A1 CA 2161973 A1 CA2161973 A1 CA 2161973A1 CA 002161973 A CA002161973 A CA 002161973A CA 2161973 A CA2161973 A CA 2161973A CA 2161973 A1 CA2161973 A1 CA 2161973A1
Authority
CA
Canada
Prior art keywords
protein
strain
glycosylation
saccharomyces cerevisiae
hlgf
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002161973A
Other languages
English (en)
French (fr)
Inventor
Joachim Ernst
Birger Jansson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Kabi Pharmacia AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabi Pharmacia AB filed Critical Kabi Pharmacia AB
Publication of CA2161973A1 publication Critical patent/CA2161973A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Endocrinology (AREA)
  • Botany (AREA)
  • Diabetes (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002161973A 1993-05-07 1994-05-06 Yeast strain and methods for expressing heterologous proteins in yeast Abandoned CA2161973A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SESE9301583-2 1993-05-07
SE19939301583A SE9301583D0 (sv) 1993-05-07 1993-05-07 Yeast strain and methods for expressing heterologous proteins in yeast

Publications (1)

Publication Number Publication Date
CA2161973A1 true CA2161973A1 (en) 1994-11-24

Family

ID=20389870

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002161973A Abandoned CA2161973A1 (en) 1993-05-07 1994-05-06 Yeast strain and methods for expressing heterologous proteins in yeast

Country Status (7)

Country Link
EP (1) EP0698086A1 (sv)
JP (1) JPH08509867A (sv)
AU (1) AU683061B2 (sv)
CA (1) CA2161973A1 (sv)
NZ (1) NZ266045A (sv)
SE (1) SE9301583D0 (sv)
WO (1) WO1994026873A1 (sv)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
CA2431013A1 (en) * 2000-12-05 2002-06-13 The Penn State Research Foundation Methods and compositions for highly efficient production of heterologous proteins in yeast
DE602005016946D1 (de) * 2004-09-02 2009-11-12 Lilly Co Eli Muteine des fibroblasten-wachstumsfaktors 21
AU2007250735A1 (en) * 2006-05-16 2007-11-22 Daiichi Sankyo Company, Limited Method for high-level secretory production of protein

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0276846A3 (en) * 1987-01-29 1989-07-26 Zymogenetics, Inc. Colony-stimulating factor derivatives
AU625392B2 (en) * 1987-10-29 1992-07-09 Zymogenetics Inc. Methods of regulating protein glycosylation

Also Published As

Publication number Publication date
SE9301583D0 (sv) 1993-05-07
JPH08509867A (ja) 1996-10-22
WO1994026873A1 (en) 1994-11-24
AU683061B2 (en) 1997-10-30
NZ266045A (en) 1997-06-24
EP0698086A1 (en) 1996-02-28
AU6662694A (en) 1994-12-12

Similar Documents

Publication Publication Date Title
EP1283265B1 (en) Process for producing protein with reduction of mannose phosphate in the sugar chain and glycoprotein produced thereby
EP1930428B1 (en) transformed yeast host cells AND METHOD OF PRODUCING FOREIGN PROTEIN
Nagasu et al. Isolation of new temperature‐sensitive mutants of Saccharomyces cerevisiae deficient in mannose outer chain elongation
US5602018A (en) Hypoglycosylated recombinant glucose oxidases
CA2161973A1 (en) Yeast strain and methods for expressing heterologous proteins in yeast
FI110006B (sv) Jästmutanter med defekter i N-glykocyleringen
KR100490190B1 (ko) 개량된 단백질 발현균주
Dittrich et al. Production and secretion of recombinant proteins in Dictyostelium discoideum
KR100227405B1 (ko) 효모 응집 유전자 및 이를 함유하는 효모
Takeda et al. Substitution at position 116 of Schizosaccharomyces pombe calmodulin decreases its stability under nitrogen starvation and results in a sporulation-deficient phenotype.
JPH0213377A (ja) デスルファトヒルジン化合物の製造方法
Wong et al. Increased expression and secretion of recombinant α-amylase in Saccharomyces cerevisiae by using glycerol as the carbon source
KR101782938B1 (ko) 사카로마이세스 세레비지애 효모의 만노스 인산화 활성에 핵심적인 유전자 mnn14 및 그 결손 변이주를 활용한 재조합 당단백질 생산 방법
US10465199B2 (en) Transformant, and method for producing transferrin
Finck et al. Defective threonine-linked glycosylation of human insulin-like growth factor in mutants of the yeast Saccharomyces cerevisiae
WO2004003205A1 (en) Hansenula polymorpha mutant strains with defect in outer chain biosynthesis and the production of recombinant glycoproteins using the same strains
Fidel Molecular analysis of the actin gene and the actin gene products of Aspergillus nidulans
US20060078965A1 (en) Chinese hamster ovary cell line transfected with 30K gene
KR20130142900A (ko) ＨｐＧＡＳ1 유전자 파쇄 효모 균주 및 이를 이용한 재조합 단백질의 생산 방법

Legal Events

Date Code Title Description
FZDE Dead