CA2151060A1 - Light scatter-based immunoassay without particle self aggregation - Google Patents
Light scatter-based immunoassay without particle self aggregationInfo
- Publication number
- CA2151060A1 CA2151060A1 CA002151060A CA2151060A CA2151060A1 CA 2151060 A1 CA2151060 A1 CA 2151060A1 CA 002151060 A CA002151060 A CA 002151060A CA 2151060 A CA2151060 A CA 2151060A CA 2151060 A1 CA2151060 A1 CA 2151060A1
- Authority
- CA
- Canada
- Prior art keywords
- microspheres
- analyte
- dimension
- histogram
- fluid sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000003018 immunoassay Methods 0.000 title claims abstract description 15
- 239000002245 particle Substances 0.000 title claims description 110
- 238000004220 aggregation Methods 0.000 title description 3
- 230000002776 aggregation Effects 0.000 title description 2
- 239000004005 microsphere Substances 0.000 claims abstract description 157
- 239000012491 analyte Substances 0.000 claims abstract description 138
- 238000009739 binding Methods 0.000 claims abstract description 87
- 230000027455 binding Effects 0.000 claims abstract description 81
- 239000002923 metal particle Substances 0.000 claims abstract description 72
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 32
- 230000000295 complement effect Effects 0.000 claims description 26
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- 230000009871 nonspecific binding Effects 0.000 claims description 6
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
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- 229910052802 copper Inorganic materials 0.000 claims description 2
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- 230000003247 decreasing effect Effects 0.000 claims description 2
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- 229930195725 Mannitol Natural products 0.000 description 6
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- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 6
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- 229910052751 metal Inorganic materials 0.000 description 5
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- 238000002965 ELISA Methods 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 238000005191 phase separation Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 238000003127 radioimmunoassay Methods 0.000 description 4
- 239000012536 storage buffer Substances 0.000 description 4
- 230000002411 adverse Effects 0.000 description 3
- 239000012131 assay buffer Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 230000002939 deleterious effect Effects 0.000 description 3
- 230000028161 membrane depolarization Effects 0.000 description 3
- 229910000027 potassium carbonate Inorganic materials 0.000 description 3
- 235000008476 powdered milk Nutrition 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 102000009843 Thyroglobulin Human genes 0.000 description 2
- 108010034949 Thyroglobulin Proteins 0.000 description 2
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- 238000001493 electron microscopy Methods 0.000 description 2
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- 235000013336 milk Nutrition 0.000 description 2
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- 229960002175 thyroglobulin Drugs 0.000 description 2
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
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- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101100381989 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) broA gene Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 208000031886 HIV Infections Diseases 0.000 description 1
- 101710123026 High molecular weight antigen Proteins 0.000 description 1
- 101000800133 Homo sapiens Thyroglobulin Proteins 0.000 description 1
- 206010020460 Human T-cell lymphotropic virus type I infection Diseases 0.000 description 1
- 241000714260 Human T-lymphotropic virus 1 Species 0.000 description 1
- 241000714259 Human T-lymphotropic virus 2 Species 0.000 description 1
- 241000713340 Human immunodeficiency virus 2 Species 0.000 description 1
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- 239000005062 Polybutadiene Substances 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
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- 101710084578 Short neurotoxin 1 Proteins 0.000 description 1
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- 101710182532 Toxin a Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
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- 230000008901 benefit Effects 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 244000309464 bull Species 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 1
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- 150000002736 metal compounds Chemical class 0.000 description 1
- 229910000000 metal hydroxide Inorganic materials 0.000 description 1
- 150000004692 metal hydroxides Chemical class 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920002857 polybutadiene Polymers 0.000 description 1
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- 235000011181 potassium carbonates Nutrition 0.000 description 1
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- 229940034208 thyroxine Drugs 0.000 description 1
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US99490392A | 1992-12-22 | 1992-12-22 | |
| US07/994,903 | 1992-12-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2151060A1 true CA2151060A1 (en) | 1994-07-07 |
Family
ID=25541198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002151060A Abandoned CA2151060A1 (en) | 1992-12-22 | 1993-12-22 | Light scatter-based immunoassay without particle self aggregation |
Country Status (9)
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6200820B1 (en) * | 1992-12-22 | 2001-03-13 | Sienna Biotech, Inc. | Light scatter-based immunoassay |
| DK0925494T3 (da) | 1996-09-04 | 2002-07-01 | Scandinavian Micro Biodevices | Mikrostrømningssystem til partikelseparation og analyse |
| US6165798A (en) * | 1996-10-10 | 2000-12-26 | University Of British Columbia | Optical quantification of analytes in membranes |
| US5858648A (en) * | 1996-11-04 | 1999-01-12 | Sienna Biotech, Inc. | Assays using reference microparticles |
| GB9707096D0 (en) * | 1997-04-08 | 1997-05-28 | Smithkline Beecham Plc | Novel device |
| EP0919568A1 (en) | 1997-12-01 | 1999-06-02 | Sorin Diagnostics S.r.l. | Escape mutant of the surface antigen of hepatitis B virus |
| US6127130A (en) | 1998-03-13 | 2000-10-03 | The United States Of America As Represented By The Secretary Of The Navy | Multiassay method for determining the concentrations of antigens and interferants |
| CA2254223A1 (en) * | 1998-11-16 | 2000-05-16 | Biophys, Inc. | Device and method for analyzing a biologic sample |
| US6221579B1 (en) | 1998-12-11 | 2001-04-24 | Kimberly-Clark Worldwide, Inc. | Patterned binding of functionalized microspheres for optical diffraction-based biosensors |
| EP1147416B1 (en) * | 1999-01-23 | 2013-05-29 | Minerva Biotechnologies Corporation | Assays involving colloids and non-colloidal structures |
| US20050148101A1 (en) * | 1999-01-23 | 2005-07-07 | Bamdad Cynthia C. | Interaction of colloid-immobilized species with species on non-colloidal structures |
| EP1169646A2 (en) * | 1999-01-25 | 2002-01-09 | Minerva Biotechnologies Corporation | Rapid and sensitive detection of aberrant protein aggregation in neurodegenerative diseases |
| FR2797690B1 (fr) * | 1999-08-20 | 2001-10-12 | Bio Merieux | Procede de detection d'un analyte et necessaire pour la mise en oeuvre de ce procede |
| US6392327B1 (en) | 2000-03-29 | 2002-05-21 | James L. Sackrison | Sonic transducer and feedback control method thereof |
| WO2001096604A2 (en) * | 2000-06-12 | 2001-12-20 | Genicon Sciences Corporation | Assay for genetic polymorphisms using scattered light detectable labels |
| ATE349011T1 (de) * | 2000-10-03 | 2007-01-15 | Minerva Biotechnologies Corp | Magnetisches in situ dilutionsverfahren |
| WO2002057743A2 (en) * | 2001-01-17 | 2002-07-25 | Streamline Proteomics, Llc. | Methods of analyzing and sorting one or more analytes |
| US7939283B2 (en) * | 2001-11-01 | 2011-05-10 | Fisher Scientific Company L.L.C. | Analyte binding turbidity assay |
| US20030119203A1 (en) | 2001-12-24 | 2003-06-26 | Kimberly-Clark Worldwide, Inc. | Lateral flow assay devices and methods for conducting assays |
| US6837171B1 (en) | 2002-04-29 | 2005-01-04 | Palmer/Snyder Furniture Company | Lightweight table with unitized table top |
| US8367013B2 (en) | 2001-12-24 | 2013-02-05 | Kimberly-Clark Worldwide, Inc. | Reading device, method, and system for conducting lateral flow assays |
| US7771922B2 (en) | 2002-05-03 | 2010-08-10 | Kimberly-Clark Worldwide, Inc. | Biomolecule diagnostic device |
| US7214530B2 (en) | 2002-05-03 | 2007-05-08 | Kimberly-Clark Worldwide, Inc. | Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices |
| US7285424B2 (en) | 2002-08-27 | 2007-10-23 | Kimberly-Clark Worldwide, Inc. | Membrane-based assay devices |
| JP4087200B2 (ja) * | 2002-09-17 | 2008-05-21 | ユニバーサル・バイオ・リサーチ株式会社 | 粒子複合体及び該粒子複合体の作製方法 |
| US7122384B2 (en) * | 2002-11-06 | 2006-10-17 | E. I. Du Pont De Nemours And Company | Resonant light scattering microparticle methods |
| CN100357737C (zh) * | 2002-11-12 | 2007-12-26 | 松下电器产业株式会社 | 特异结合反应测定方法、测定装置以及其中所用试剂盒 |
| US7781172B2 (en) | 2003-11-21 | 2010-08-24 | Kimberly-Clark Worldwide, Inc. | Method for extending the dynamic detection range of assay devices |
| US7247500B2 (en) | 2002-12-19 | 2007-07-24 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in membrane-based assay devices |
| US20040197819A1 (en) | 2003-04-03 | 2004-10-07 | Kimberly-Clark Worldwide, Inc. | Assay devices that utilize hollow particles |
| US7851209B2 (en) | 2003-04-03 | 2010-12-14 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in assay devices |
| US20050112703A1 (en) | 2003-11-21 | 2005-05-26 | Kimberly-Clark Worldwide, Inc. | Membrane-based lateral flow assay devices that utilize phosphorescent detection |
| US7943395B2 (en) | 2003-11-21 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Extension of the dynamic detection range of assay devices |
| US7713748B2 (en) | 2003-11-21 | 2010-05-11 | Kimberly-Clark Worldwide, Inc. | Method of reducing the sensitivity of assay devices |
| WO2005057169A2 (en) * | 2003-12-05 | 2005-06-23 | Trellis Bioscience, Inc. | Homogeneous competition assays |
| US7943089B2 (en) | 2003-12-19 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Laminated assay devices |
| US7815854B2 (en) | 2004-04-30 | 2010-10-19 | Kimberly-Clark Worldwide, Inc. | Electroluminescent illumination source for optical detection systems |
| US7796266B2 (en) | 2004-04-30 | 2010-09-14 | Kimberly-Clark Worldwide, Inc. | Optical detection system using electromagnetic radiation to detect presence or quantity of analyte |
| US20070259380A1 (en) * | 2004-05-20 | 2007-11-08 | Kyoichi Sumida | Method for Measuring Hyaluronic Acid Using Hyaluronic Acid Binding Protein |
| US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
| US7170035B2 (en) * | 2004-08-04 | 2007-01-30 | Hottboxx Llc | Heated construction box |
| ITMI20041800A1 (it) * | 2004-09-21 | 2004-12-21 | Univ Degli Studi Milano | Netodo di misurazione di interazioni molecolari con lls |
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| PL1843148T3 (pl) * | 2006-04-08 | 2022-11-21 | F.Hoffmann-La Roche Ag | Analiza danych optycznych za pomocą histogramów |
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| US9618523B2 (en) * | 2013-02-28 | 2017-04-11 | Siemens Healthcare Diagnostics Inc. | Methods and reagents for determining isomeric analytes |
| US20150046862A1 (en) * | 2013-08-11 | 2015-02-12 | Silicon Graphics International Corp. | Modifying binning operations |
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| US3873204A (en) * | 1970-01-14 | 1975-03-25 | Bio Physics Systems Inc | Optical extinction photoanalysis apparatus for small particles |
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| US5286452A (en) * | 1991-05-20 | 1994-02-15 | Sienna Biotech, Inc. | Simultaneous multiple assays |
-
1993
- 1993-06-15 TW TW082104758A patent/TW239881B/zh active
- 1993-12-21 CN CN93119931A patent/CN1061142C/zh not_active Expired - Fee Related
- 1993-12-22 KR KR1019950702661A patent/KR0171452B1/ko not_active IP Right Cessation
- 1993-12-22 EP EP94904875A patent/EP0676045A4/en not_active Withdrawn
- 1993-12-22 CA CA002151060A patent/CA2151060A1/en not_active Abandoned
- 1993-12-22 JP JP6515382A patent/JPH08505231A/ja active Pending
- 1993-12-22 AU AU58734/94A patent/AU670489B2/en not_active Ceased
- 1993-12-22 WO PCT/US1993/012428 patent/WO1994015193A1/en not_active Application Discontinuation
-
1994
- 1994-08-05 US US08/286,778 patent/US5589401A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| KR0171452B1 (ko) | 1999-05-01 |
| US5589401A (en) | 1996-12-31 |
| EP0676045A1 (en) | 1995-10-11 |
| EP0676045A4 (en) | 1998-02-04 |
| AU5873494A (en) | 1994-07-19 |
| AU670489B2 (en) | 1996-07-18 |
| WO1994015193A1 (en) | 1994-07-07 |
| CN1061142C (zh) | 2001-01-24 |
| JPH08505231A (ja) | 1996-06-04 |
| TW239881B (US20060003336A1-20060105-C00008.png) | 1995-02-01 |
| CN1092866A (zh) | 1994-09-28 |
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| Date | Code | Title | Description |
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| EEER | Examination request | ||
| FZDE | Discontinued |