CA2115061A1 - Methode d'isolement d'adn - Google Patents

Methode d'isolement d'adn

Info

Publication number
CA2115061A1
CA2115061A1 CA002115061A CA2115061A CA2115061A1 CA 2115061 A1 CA2115061 A1 CA 2115061A1 CA 002115061 A CA002115061 A CA 002115061A CA 2115061 A CA2115061 A CA 2115061A CA 2115061 A1 CA2115061 A1 CA 2115061A1
Authority
CA
Canada
Prior art keywords
dna
biological sample
lysis
kinetoplast
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002115061A
Other languages
English (en)
Inventor
David S. Sigman
Larry Simpson
Herbert Avila
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WORLD HEALTH ORGANISATION (THE)
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2115061A1 publication Critical patent/CA2115061A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002115061A 1991-08-06 1992-08-04 Methode d'isolement d'adn Abandoned CA2115061A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74110991A 1991-08-06 1991-08-06
US741,109 1991-08-06

Publications (1)

Publication Number Publication Date
CA2115061A1 true CA2115061A1 (fr) 1993-02-18

Family

ID=24979431

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002115061A Abandoned CA2115061A1 (fr) 1991-08-06 1992-08-04 Methode d'isolement d'adn

Country Status (4)

Country Link
JP (1) JPH07504801A (fr)
BR (1) BR9206341A (fr)
CA (1) CA2115061A1 (fr)
WO (1) WO1993003167A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2137563C (fr) * 1992-06-08 2005-06-28 Thomas B. Ryder Obtention d'acides nucleiques a partir de cellules mononucleees
FR2705358B1 (fr) 1993-05-13 1995-08-04 Orstom Procédé de culture in vitro de différents stades de parasites tissulaires, stades parasitaires obtenus et applications biologiques.
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JPH08275799A (ja) * 1995-04-04 1996-10-22 Sumitomo Electric Ind Ltd リーシュマニアの検出用ポリヌクレオチド及びリーシュマニア原虫の検出方法
ZA973642B (en) 1996-04-26 1997-11-25 Merck & Co Inc DNA vaccine formulations.
JP4386965B2 (ja) * 1996-04-26 2009-12-16 メルク エンド カンパニー インコーポレーテッド Ｄｎａワクチン製剤
US6210881B1 (en) 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
EP1038028A2 (fr) * 1997-12-10 2000-09-27 Sierra Diagnostics, Inc. Procedes et reactifs de conservation de l'adn de fluides corporels
US7569342B2 (en) * 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP1207208A3 (fr) * 2000-11-15 2003-12-10 Becton Dickinson and Company Méthode pour la préservation des cellules et des acides nucléiques cibles
WO2016136324A1 (fr) * 2015-02-24 2016-09-01 東洋紡株式会社 Procédé de stabilisation d'une sonde dans une solution de réaction de détection d'acides nucléiques
EP3568475B1 (fr) 2017-01-16 2023-03-08 Spectrum Solutions L.L.C. Solution de conservation d'acides nucléiques et ses procédés d'utilisation
CN106868121B (zh) * 2017-02-15 2020-11-06 中山大学 一种检测路氏锥虫的引物对和试剂盒
AU2018234638B2 (en) 2017-03-15 2023-04-06 Ancestry.Com Dna, Llc Sample collection device and method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130423A (en) * 1990-07-13 1992-07-14 Microprobe Corporation Non-corrosive compositions and methods useful for the extraction of nucleic acids

Also Published As

Publication number Publication date
BR9206341A (pt) 1994-11-08
JPH07504801A (ja) 1995-06-01
WO1993003167A1 (fr) 1993-02-18

Similar Documents

Publication Publication Date Title
CA2115061A1 (fr) Methode d'isolement d'adn
Avila et al. Polymerase chain reaction amplification of Trypanosoma cruzi kinetoplast minicircle DNA isolated from whole blood lysates: diagnosis of chronic Chagas' disease
EP0887427B1 (fr) Amplification et détection du HIV-1 et/ou HIV-2
EP0305399B1 (fr) Procede chaotropique d'evaluation d'acides nucleiques dans un specimen biologique
Waters et al. Rapid, sensitive diagnosis of malaria based on ribosomal RNA
EP0235726B1 (fr) Détection rapide des séquences d'acides nucléique dans un échantillon par marquage de l'échantillon
Widmer Genetic heterogeneity and PCR detection of Cryptosporidium parvum
EP0656955B1 (fr) Elimination de faux negatifs en depistage d'acides nucleiques
EP2527475B1 (fr) Amorces pour la détection de plasmodium
EA005577B1 (ru) Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
Rochelle et al. Extraction and amplification of 16S rRNA genes from deep marine sediments and seawater to assess bacterial community diversity
JPH02503515A (ja) 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
EP0269445A2 (fr) Procédé de détection des virus HTLVI et HTLVII par hybridation
US11015185B1 (en) Blood cell lysis reagent
US6605439B2 (en) Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
Brandon et al. Early detection of bovine leukosis virus DNA in infected sheep using the polymerase chain reaction
JP2000511058A (ja) Ｍｙｃｏｂａｃｔｅｒｉｕｍ Ｋａｎｓａｓｉｉを検出するための組成物および方法
WO1994012657A1 (fr) Methodes de pcr par adjonction directe de phenol, de transcription inverse (tr) et de pcr-tr
JP4503712B2 (ja) 核酸合成法
JP4186270B2 (ja) 核酸合成法
Buchanan et al. Extraction of DNA from frozen red blood cells
EP0658621A1 (fr) Elimination enzymatique d'acide nucléique non ciblée des échantillons biologiques
Stefano et al. Rapid and sensitive detection ofChlamydia trachomatisusing a ligatable binary RNA probe and Qβ replicase
US20040142327A1 (en) Methods for identifying and isolating DNA polymorphisms or genetic markers
JPH06253847A (ja) 炭疽菌検出のためのオリゴヌクレオチドおよびそれを用いた検出方法

Legal Events

Date Code Title Description
FZDE Dead