CA2089752A1 - Production of human lysozyme in methylotrophic yeast cells and efficient secretion therefrom - Google Patents

Production of human lysozyme in methylotrophic yeast cells and efficient secretion therefrom

Info

Publication number
CA2089752A1
CA2089752A1 CA 2089752 CA2089752A CA2089752A1 CA 2089752 A1 CA2089752 A1 CA 2089752A1 CA 2089752 CA2089752 CA 2089752 CA 2089752 A CA2089752 A CA 2089752A CA 2089752 A1 CA2089752 A1 CA 2089752A1
Authority
CA
Canada
Prior art keywords
hlz
dna
gene
methylotrophic yeast
dna fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2089752
Other languages
English (en)
French (fr)
Inventor
Geneva R. Davis
Cathy A. Stillman
Russell A. Brierley
Gregory P. Thill
Thomas S. Vedvick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2089752A1 publication Critical patent/CA2089752A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA 2089752 1990-09-05 1991-09-04 Production of human lysozyme in methylotrophic yeast cells and efficient secretion therefrom Abandoned CA2089752A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57799490A 1990-09-05 1990-09-05
US577,994 1990-09-05

Publications (1)

Publication Number Publication Date
CA2089752A1 true CA2089752A1 (en) 1992-03-06

Family

ID=24311014

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2089752 Abandoned CA2089752A1 (en) 1990-09-05 1991-09-04 Production of human lysozyme in methylotrophic yeast cells and efficient secretion therefrom

Country Status (4)

Country Link
EP (1) EP0547171A1 (ja)
JP (1) JPH06503718A (ja)
CA (1) CA2089752A1 (ja)
WO (1) WO1992004441A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104278017A (zh) * 2013-07-11 2015-01-14 上海万特医药科技有限公司 一种重组表达人溶菌酶的方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
CA2105064A1 (en) * 1991-04-01 1992-10-02 Martin Anthony Gleeson Genes which influence pichia proteolytic activity, and uses therefor
JPH06209763A (ja) * 1993-01-13 1994-08-02 Green Cross Corp:The 変異株
CN105039189B (zh) * 2015-06-23 2018-04-17 山东新概念生物技术有限公司 一种产鸡溶菌酶的基因工程菌及其构建与应用

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104278017A (zh) * 2013-07-11 2015-01-14 上海万特医药科技有限公司 一种重组表达人溶菌酶的方法

Also Published As

Publication number Publication date
EP0547171A1 (en) 1993-06-23
WO1992004441A1 (en) 1992-03-19
JPH06503718A (ja) 1994-04-28

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Legal Events

Date Code Title Description
FZDE Dead