CA2050276C - Reactions d'allongement d'amorce - Google Patents

Reactions d'allongement d'amorce Download PDF

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Publication number
CA2050276C
CA2050276C CA002050276A CA2050276A CA2050276C CA 2050276 C CA2050276 C CA 2050276C CA 002050276 A CA002050276 A CA 002050276A CA 2050276 A CA2050276 A CA 2050276A CA 2050276 C CA2050276 C CA 2050276C
Authority
CA
Canada
Prior art keywords
dna
agent
dna polymerase
polymerase
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002050276A
Other languages
English (en)
Other versions
CA2050276A1 (fr
Inventor
Stanley Tabor
Charles C. Richardson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of CA2050276A1 publication Critical patent/CA2050276A1/fr
Application granted granted Critical
Publication of CA2050276C publication Critical patent/CA2050276C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
CA002050276A 1989-04-12 1990-04-10 Reactions d'allongement d'amorce Expired - Lifetime CA2050276C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33675189A 1989-04-12 1989-04-12
US336,751 1989-04-12
PCT/US1990/001938 WO1990012111A1 (fr) 1989-04-12 1990-04-10 Reactions d'extension d'amorce ameliorees

Publications (2)

Publication Number Publication Date
CA2050276A1 CA2050276A1 (fr) 1990-10-13
CA2050276C true CA2050276C (fr) 2003-03-11

Family

ID=23317491

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002050276A Expired - Lifetime CA2050276C (fr) 1989-04-12 1990-04-10 Reactions d'allongement d'amorce

Country Status (8)

Country Link
EP (1) EP0467953A4 (fr)
JP (1) JP2997043B2 (fr)
KR (1) KR920700294A (fr)
AU (1) AU638246B2 (fr)
CA (1) CA2050276C (fr)
HU (1) HUT61054A (fr)
LT (1) LTIP1519A (fr)
WO (1) WO1990012111A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
FR2674254B1 (fr) * 1991-03-20 1995-10-06 Univ Reims Champagne Ardenne Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.
US5256555A (en) * 1991-12-20 1993-10-26 Ambion, Inc. Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
FI923911A (fi) * 1992-09-01 1994-03-02 Vsevolod Kiselev DNA-molekylers in vitro-syntes
DE4336266A1 (de) * 1993-10-23 1995-04-27 Boehringer Mannheim Gmbh Stabilisierte flüssige Mischungen für die Markierung von Nukleinsäuren
WO1996038568A1 (fr) * 1995-05-31 1996-12-05 Amersham Life Science, Inc. Adn-polymerases thermostables
US5665551A (en) * 1995-09-13 1997-09-09 Roche Molecular Systems, Inc. Purified nucleic acid encoding a thermostable pyrophosphatase
DE19612779A1 (de) * 1996-03-29 1997-10-02 Boehringer Mannheim Gmbh Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR
WO1998015655A1 (fr) * 1996-10-07 1998-04-16 The Perkin-Elmer Corporation Reaction d'extension d'amorce utilisant une paire d'enzymes d'un meme substrat pour consommer le pyrophosphate
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
AU2004277589A1 (en) * 2003-09-30 2005-04-14 Perkinelmer Las, Inc. Compositions and processes for genotyping single nucleotide polymorphisms
WO2014187924A1 (fr) * 2013-05-24 2014-11-27 Illumina Cambridge Limited Sequençage pyrophosphorolytique
EP4278005A1 (fr) 2021-01-14 2023-11-22 Integrated DNA Technologies, Inc. Procédés de production et de quantification de billes marquées par un identifiant moléculaire unique

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1338457C (fr) * 1986-08-22 1996-07-16 Henry A. Erlich Enzyme thermostable purifiee
US4971903A (en) * 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids

Also Published As

Publication number Publication date
CA2050276A1 (fr) 1990-10-13
HU903562D0 (en) 1992-03-30
EP0467953A4 (en) 1992-06-17
JPH04506002A (ja) 1992-10-22
WO1990012111A1 (fr) 1990-10-18
AU5438290A (en) 1990-11-05
AU638246B2 (en) 1993-06-24
LTIP1519A (en) 1995-06-26
JP2997043B2 (ja) 2000-01-11
EP0467953A1 (fr) 1992-01-29
HUT61054A (en) 1992-11-30
KR920700294A (ko) 1992-02-19

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