CA2045614A1 - Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe - Google Patents

Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe

Info

Publication number
CA2045614A1
CA2045614A1 CA2045614A CA2045614A CA2045614A1 CA 2045614 A1 CA2045614 A1 CA 2045614A1 CA 2045614 A CA2045614 A CA 2045614A CA 2045614 A CA2045614 A CA 2045614A CA 2045614 A1 CA2045614 A1 CA 2045614A1
Authority
CA
Canada
Prior art keywords
cell sample
proliferation
cell
images
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2045614A
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English (en)
Other versions
CA2045614C (fr
Inventor
James W. Bacus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Analysis Systems Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/315,289 external-priority patent/US5086476A/en
Application filed by Individual filed Critical Individual
Publication of CA2045614A1 publication Critical patent/CA2045614A1/fr
Application granted granted Critical
Publication of CA2045614C publication Critical patent/CA2045614C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Méthode et appareil de traitement des images (10) donnant un indice de prolifération d'un échantillon de cellules par coloration des cellules à l'aide d'un chromogène comme substance indicative de la prolifération et par contre-coloration pour le dénombrement des noyaux des cellules. Le chromogène est activé par un conjugué anticorps-enzyme qui se lie à la substance de prolifération pour produire un échantillon de cellules colorées. L'échantillon de cellules colorées est observé à l'aide d'un microscope optique (12), faisant partie intégrante de l'appareil, qui donne une image agrandie de l'échantillon de cellules. L'appareil filtre optiquement (18) l'image de l'échantillon et produit une paire d'images optiquement améliorées de la substance de prolifération et du noyau des cellules. Les images améliorées sont analysées électroniquement pour déterminer le nombre de noyaux de cellules et la quantité de substance de prolifération apparaissant dans les images respectivement. Les résultats sont alors comparés pour créer un indice de prolifération pour la portion de l'échantillon de cellules apparaissant dans l'image de l'échantillon.
CA002045614A 1989-02-24 1990-02-23 Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe Expired - Fee Related CA2045614C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/315,289 US5086476A (en) 1985-11-04 1989-02-24 Method and apparatus for determining a proliferation index of a cell sample
US315,289 1989-02-24

Publications (2)

Publication Number Publication Date
CA2045614A1 true CA2045614A1 (fr) 1990-08-25
CA2045614C CA2045614C (fr) 1997-09-30

Family

ID=23223738

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002045614A Expired - Fee Related CA2045614C (fr) 1989-02-24 1990-02-23 Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe

Country Status (3)

Country Link
EP (1) EP0460071A4 (fr)
CA (1) CA2045614C (fr)
WO (1) WO1990010277A1 (fr)

Families Citing this family (30)

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US5889881A (en) * 1992-10-14 1999-03-30 Oncometrics Imaging Corp. Method and apparatus for automatically detecting malignancy-associated changes
US6026174A (en) * 1992-10-14 2000-02-15 Accumed International, Inc. System and method for automatically detecting malignant cells and cells having malignancy-associated changes
MXPA06008496A (es) 2004-02-02 2007-01-30 Ambrx Inc Polipeptidos de interferon humano modificados y sus usos.
MXPA06014684A (es) 2004-06-18 2007-02-12 Ambrx Inc Novedosos polipeptidos de enlace antigeno y sus usos.
WO2009100255A2 (fr) 2008-02-08 2009-08-13 Ambrx, Inc. Polypeptides leptine modifiés et leurs utilisations
US7638491B2 (en) 2004-12-22 2009-12-29 Ambrx, Inc. Therapies using non-natural amino acids and polypeptides
MX2007007580A (es) 2004-12-22 2007-12-11 Ambrx Inc Hormona del crecimiento humana modificada.
ATE541934T1 (de) 2004-12-22 2012-02-15 Ambrx Inc Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon
KR101224781B1 (ko) 2004-12-22 2013-01-21 암브룩스, 인코포레이티드 비천연적으로 코딩된 아미노산을 포함하는 인간 성장호르몬의 조제물
BRPI0518661A2 (pt) 2004-12-22 2008-12-02 Ambrx Inc mÉtodos para expressço e purificaÇço do hormânio do crescimento humano recombinante
EP1893229B1 (fr) 2005-06-03 2011-10-19 Ambrx, Inc. Molecules d'interferons humains ameliorees et utilisations de celles-ci
PT2339014E (pt) 2005-11-16 2015-10-13 Ambrx Inc Métodos e composições compreendendo aminoácidos não-naturais
US8420792B2 (en) 2006-09-08 2013-04-16 Ambrx, Inc. Suppressor tRNA transcription in vertebrate cells
CN106008699A (zh) 2006-09-08 2016-10-12 Ambrx公司 经修饰的人类血浆多肽或Fc骨架和其用途
CA2662753C (fr) 2006-09-08 2016-02-23 Ambrx, Inc. Arnt suppresseur hybride pour cellules de vertebres
EP2068909B1 (fr) 2007-03-30 2012-04-25 Ambrx, Inc. Polypeptides fgf-21 modifies, et leurs utilisations
AU2008247815B2 (en) 2007-05-02 2012-09-06 Ambrx, Inc. Modified interferon beta polypeptides and their uses
JP5547083B2 (ja) 2007-11-20 2014-07-09 アンブルックス，インコーポレイテッド 修飾されたインスリンポリペプチドおよびそれらの使用
US10138283B2 (en) 2008-07-23 2018-11-27 Ambrx, Inc. Modified bovine G-CSF polypeptides and their uses
MX348657B (es) 2008-09-26 2017-06-21 Ambrx Inc Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales.
CN107022020A (zh) 2008-09-26 2017-08-08 Ambrx公司 修饰的动物促红细胞生成素多肽和其用途
CA2784800A1 (fr) 2009-12-21 2011-07-21 Ambrx, Inc. Polypeptides d'hormone de croissance porcine modifiee et leurs utilisations
AU2010341516B2 (en) 2009-12-21 2014-01-16 Ambrx, Inc. Modified bovine somatotropin polypeptides and their uses
SG10201506443TA (en) 2010-08-17 2015-10-29 Ambrx Inc Modified relaxin polypeptides and their uses
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
JP6355082B2 (ja) 2013-07-18 2018-07-11 パナソニックＩｐマネジメント株式会社 病理診断支援装置及び病理診断支援方法
LT3412302T (lt) 2014-10-24 2021-07-26 Bristol-Myers Squibb Company Modifikuoti fgf-21 polipeptidai ir jų panaudojimai
US10266578B2 (en) 2017-02-08 2019-04-23 Bristol-Myers Squibb Company Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof
CN111413504B (zh) * 2020-04-03 2022-01-28 河北医科大学第四医院 一种辅助判读ki67增殖指数的标准比对卡

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US3999047A (en) * 1972-09-05 1976-12-21 Green James E Method and apparatus utilizing color algebra for analyzing scene regions
US4199748A (en) * 1976-11-01 1980-04-22 Rush-Presbyterian-St. Luke's Medical Center Automated method and apparatus for classification of cells with application to the diagnosis of anemia
JPS5661650A (en) * 1979-10-24 1981-05-27 Omron Tateisi Electronics Co Analyzing device of cell
US4741043B1 (en) * 1985-11-04 1994-08-09 Cell Analysis Systems Inc Method of and apparatus for image analyses of biological specimens

Also Published As

Publication number Publication date
WO1990010277A1 (fr) 1990-09-07
EP0460071A1 (fr) 1991-12-11
EP0460071A4 (en) 1993-02-03
CA2045614C (fr) 1997-09-30

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