CA2045614A1 - Method and apparatus for determining a proliferation index of a cell sample - Google Patents

Method and apparatus for determining a proliferation index of a cell sample

Info

Publication number
CA2045614A1
CA2045614A1 CA2045614A CA2045614A CA2045614A1 CA 2045614 A1 CA2045614 A1 CA 2045614A1 CA 2045614 A CA2045614 A CA 2045614A CA 2045614 A CA2045614 A CA 2045614A CA 2045614 A1 CA2045614 A1 CA 2045614A1
Authority
CA
Canada
Prior art keywords
cell sample
proliferation
cell
images
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2045614A
Other languages
French (fr)
Other versions
CA2045614C (en
Inventor
James W. Bacus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Analysis Systems Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/315,289 external-priority patent/US5086476A/en
Application filed by Individual filed Critical Individual
Publication of CA2045614A1 publication Critical patent/CA2045614A1/en
Application granted granted Critical
Publication of CA2045614C publication Critical patent/CA2045614C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An image processing method and apparatus (10) determines a proliferation index of a cell sample by staining the cells with a chromogen for a proliferation substance and a counterstain for the cell nuclei. The chromogen is activated by an antibody-enzyme conjugate which binds to the proliferation substance to produce a stained cell sample. The stained cell sample is examined with an optical microscope (12), forming a portion of the apparatus, which produces a magnified cell sample image. The apparatus optically filters (18) the cell sample image and produces a pair of optically enhanced proliferation substance and cell nuclei images. The enhanced images are electronically analyzed to determine the amounts of cell nuclei and proliferation substance appearing in the images, respectively. The amounts are then compared to yield a proliferation index for the portion of the cell sample appearing in the cell sample image.
CA002045614A 1989-02-24 1990-02-23 Method and apparatus for determining a proliferation index of a cell sample Expired - Fee Related CA2045614C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/315,289 US5086476A (en) 1985-11-04 1989-02-24 Method and apparatus for determining a proliferation index of a cell sample
US315,289 1989-02-24

Publications (2)

Publication Number Publication Date
CA2045614A1 true CA2045614A1 (en) 1990-08-25
CA2045614C CA2045614C (en) 1997-09-30

Family

ID=23223738

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002045614A Expired - Fee Related CA2045614C (en) 1989-02-24 1990-02-23 Method and apparatus for determining a proliferation index of a cell sample

Country Status (3)

Country Link
EP (1) EP0460071A4 (en)
CA (1) CA2045614C (en)
WO (1) WO1990010277A1 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5889881A (en) * 1992-10-14 1999-03-30 Oncometrics Imaging Corp. Method and apparatus for automatically detecting malignancy-associated changes
US6026174A (en) * 1992-10-14 2000-02-15 Accumed International, Inc. System and method for automatically detecting malignant cells and cells having malignancy-associated changes
BRPI0507169A (en) 2004-02-02 2007-06-26 Ambrx Inc modified human growth hormone polypeptides and their uses
KR20070034512A (en) 2004-06-18 2007-03-28 암브룩스, 인코포레이티드 New Antigen-Binding Polypeptides and Uses thereof
CA2594561C (en) 2004-12-22 2014-12-23 Ambrx, Inc. Formulations of human growth hormone comprising a non-naturally encoded amino acid
AU2005323106B2 (en) 2004-12-22 2010-07-29 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
CA2594557C (en) 2004-12-22 2016-04-26 Ambrx, Inc. Modified human growth hormone
WO2006068802A2 (en) 2004-12-22 2006-06-29 Ambrx, Inc. COMPOSITIONS OF AMINOACYL-tRNA SYNTHETASE AND USES THEREOF
EP2399893B1 (en) 2004-12-22 2018-08-15 Ambrx, Inc. Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
WO2006133089A2 (en) 2005-06-03 2006-12-14 Ambrx, Inc. Improved human interferon molecules and their uses
CN101454461A (en) 2005-11-16 2009-06-10 Ambrx公司 Methods and compositions comprising non-natural amino acids
JP5451390B2 (en) 2006-09-08 2014-03-26 アンブルックス，インコーポレイテッド Transcription of suppressor TRNA in vertebrate cells
KR20090051227A (en) 2006-09-08 2009-05-21 암브룩스, 인코포레이티드 Hybrid suppressor trna for vertebrate cells
CN106008699A (en) 2006-09-08 2016-10-12 Ambrx公司 Modified human plasma polypeptide or Fc scaffolds and their uses
KR101476472B1 (en) 2007-03-30 2015-01-05 암브룩스, 인코포레이티드 Modified fgf-21 polypeptides and their uses
MX2009011870A (en) 2007-05-02 2009-11-12 Ambrx Inc Modified interferon beta polypeptides and their uses.
ES2632504T3 (en) 2007-11-20 2017-09-13 Ambrx, Inc. Modified insulin polypeptides and their uses
NZ602170A (en) 2008-02-08 2014-03-28 Ambrx Inc Modified leptin polypeptides and their uses
JP5680534B2 (en) 2008-07-23 2015-03-04 イーライ リリー アンド カンパニー Modified bovine G-CSF polypeptides and their use
ES2660000T3 (en) 2008-09-26 2018-03-20 Ambrx, Inc. Vaccines and microorganisms dependent on the replication of unnatural amino acids
MX2011003272A (en) 2008-09-26 2011-04-28 Ambrx Inc Modified animal erythropoietin polypeptides and their uses.
EP2805965A1 (en) 2009-12-21 2014-11-26 Ambrx, Inc. Modified porcine somatotropin polypeptides and their uses
JP2013515080A (en) 2009-12-21 2013-05-02 アンブルックス，インコーポレイテッド Modified bovine somatotropin polypeptides and their use
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
ES2972902T3 (en) 2010-08-17 2024-06-17 Ambrx Inc Modified relaxin polypeptides and their uses
TWI480288B (en) 2010-09-23 2015-04-11 Lilly Co Eli Formulations for bovine granulocyte colony stimulating factor and variants thereof
JP6355082B2 (en) 2013-07-18 2018-07-11 パナソニックＩｐマネジメント株式会社 Pathological diagnosis support apparatus and pathological diagnosis support method
AU2015335603B2 (en) 2014-10-24 2020-04-30 Bristol-Myers Squibb Company Modified FGF-21 polypeptides and uses thereof
AU2018219283B2 (en) 2017-02-08 2022-05-19 Bristol-Myers Squibb Company Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof
CN111413504B (en) * 2020-04-03 2022-01-28 河北医科大学第四医院 Standard comparison card for assisting interpretation of KI67 proliferation index

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US3999047A (en) * 1972-09-05 1976-12-21 Green James E Method and apparatus utilizing color algebra for analyzing scene regions
US4199748A (en) * 1976-11-01 1980-04-22 Rush-Presbyterian-St. Luke's Medical Center Automated method and apparatus for classification of cells with application to the diagnosis of anemia
JPS5661650A (en) * 1979-10-24 1981-05-27 Omron Tateisi Electronics Co Analyzing device of cell
US4741043B1 (en) * 1985-11-04 1994-08-09 Cell Analysis Systems Inc Method of and apparatus for image analyses of biological specimens

Also Published As

Publication number Publication date
EP0460071A4 (en) 1993-02-03
CA2045614C (en) 1997-09-30
WO1990010277A1 (en) 1990-09-07
EP0460071A1 (en) 1991-12-11

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