CA2015707A1 - Maturation in vitro des oocytes bovins - Google Patents
Maturation in vitro des oocytes bovinsInfo
- Publication number
- CA2015707A1 CA2015707A1 CA002015707A CA2015707A CA2015707A1 CA 2015707 A1 CA2015707 A1 CA 2015707A1 CA 002015707 A CA002015707 A CA 002015707A CA 2015707 A CA2015707 A CA 2015707A CA 2015707 A1 CA2015707 A1 CA 2015707A1
- Authority
- CA
- Canada
- Prior art keywords
- oocytes
- bovine
- oocyte
- maturation
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 107
- 241000283690 Bos taurus Species 0.000 title claims abstract description 51
- 230000035800 maturation Effects 0.000 title claims abstract description 47
- 238000000338 in vitro Methods 0.000 title claims description 49
- 238000000034 method Methods 0.000 claims abstract description 41
- 238000003501 co-culture Methods 0.000 claims abstract description 25
- 238000012546 transfer Methods 0.000 claims abstract description 22
- 210000004027 cell Anatomy 0.000 claims abstract description 15
- 102000006771 Gonadotropins Human genes 0.000 claims abstract description 13
- 108010086677 Gonadotropins Proteins 0.000 claims abstract description 13
- 239000002622 gonadotropin Substances 0.000 claims abstract description 13
- 229940094892 gonadotropins Drugs 0.000 claims abstract description 13
- 108010077544 Chromatin Proteins 0.000 claims description 11
- 210000003483 chromatin Anatomy 0.000 claims description 11
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims description 6
- 210000001672 ovary Anatomy 0.000 claims description 6
- 229960005309 estradiol Drugs 0.000 claims description 5
- 229930182833 estradiol Natural products 0.000 claims description 5
- 210000004340 zona pellucida Anatomy 0.000 claims description 5
- 230000003115 biocidal effect Effects 0.000 claims description 4
- 210000001771 cumulus cell Anatomy 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 2
- 230000000977 initiatory effect Effects 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 150000004695 complexes Chemical class 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract 1
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 13
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 13
- 230000004720 fertilization Effects 0.000 description 13
- 229940028334 follicle stimulating hormone Drugs 0.000 description 13
- 238000011161 development Methods 0.000 description 11
- 230000018109 developmental process Effects 0.000 description 11
- 210000002459 blastocyst Anatomy 0.000 description 10
- 102000009151 Luteinizing Hormone Human genes 0.000 description 9
- 108010073521 Luteinizing Hormone Proteins 0.000 description 9
- 210000002257 embryonic structure Anatomy 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 230000004913 activation Effects 0.000 description 8
- 229940040129 luteinizing hormone Drugs 0.000 description 8
- 239000003104 tissue culture media Substances 0.000 description 8
- 210000000472 morula Anatomy 0.000 description 7
- 239000011824 nuclear material Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- 229930182555 Penicillin Natural products 0.000 description 5
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 229940049954 penicillin Drugs 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 4
- 238000010449 nuclear transplantation Methods 0.000 description 4
- 230000035935 pregnancy Effects 0.000 description 4
- 238000010867 Hoechst staining Methods 0.000 description 3
- 230000013020 embryo development Effects 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 210000002503 granulosa cell Anatomy 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- QTENRWWVYAAPBI-YZTFXSNBSA-N Streptomycin sulfate Chemical compound OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@H]1[C@H](N=C(N)N)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]1O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@H]1[C@H](N=C(N)N)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]1O QTENRWWVYAAPBI-YZTFXSNBSA-N 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 208000010643 digestive system disease Diseases 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 208000017169 kidney disease Diseases 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 238000011177 media preparation Methods 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 230000031864 metaphase Effects 0.000 description 2
- 230000002611 ovarian Effects 0.000 description 2
- 210000003101 oviduct Anatomy 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000001817 pituitary effect Effects 0.000 description 2
- 210000004508 polar body Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
- 206010016825 Flushing Diseases 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000001109 blastomere Anatomy 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- SMNPLAKEGAEPJD-UHFFFAOYSA-N chembl34922 Chemical compound Cl.Cl.Cl.C1CN(C)CCN1C1=CC=C(NC(=N2)C=3C=C4N=C(NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 SMNPLAKEGAEPJD-UHFFFAOYSA-N 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000001158 estrous effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000012248 genetic selection Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004619 light microscopy Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000010198 maturation time Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 230000008182 oocyte development Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000002684 recombinant hormone Substances 0.000 description 1
- 238000009394 selective breeding Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0609—Oocytes, oogonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/31—Pituitary sex hormones, e.g. follicle-stimulating hormone [FSH], luteinising hormone [LH]; Chorionic gonadotropins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2517/00—Cells related to new breeds of animals
- C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US345,402 | 1982-02-03 | ||
US34540289A | 1989-05-01 | 1989-05-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2015707A1 true CA2015707A1 (fr) | 1990-11-01 |
Family
ID=23354903
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002015707A Abandoned CA2015707A1 (fr) | 1989-05-01 | 1990-04-30 | Maturation in vitro des oocytes bovins |
Country Status (4)
Country | Link |
---|---|
AU (1) | AU5531090A (fr) |
CA (1) | CA2015707A1 (fr) |
IL (1) | IL94184A0 (fr) |
WO (1) | WO1990013627A1 (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE881133L (en) * | 1988-04-14 | 1989-10-14 | Univ Dublin | In-vitro culture of bovine embryos |
CA2072475A1 (fr) * | 1991-07-01 | 1993-01-02 | Tanja Dominko | Maturation in vitro d'ovocytes bovins denudes |
AU667507B2 (en) * | 1992-03-04 | 1996-03-28 | Abs Global, Inc. | Improved procedure for bovine nuclear transfer |
US6107543A (en) * | 1992-08-20 | 2000-08-22 | Infigen, Inc. | Culture of totipotent embryonic inner cells mass cells and production of bovine animals |
US5563059A (en) * | 1993-02-23 | 1996-10-08 | Genentech, Inc. | Use of human inhibin and human activin to increase the number of mature primate oocytes |
CA2223125A1 (fr) * | 1995-02-17 | 1996-08-22 | Stichting Instituut Voor Dierhouderij En Diergezondheid | Production de folliculostimuline bovine recombinante (recbfsh) a activi te biologique dans le systeme d'expression de baculovirus |
WO2001068831A2 (fr) | 2000-03-15 | 2001-09-20 | The University Of Georgia Research Foundation, Inc. | Reprogrammation effective de materiel nucleaire chez des mammiferes |
US20050208651A1 (en) | 2002-03-08 | 2005-09-22 | Ri-Cheng Chian | Vitro maturation of immature human oocytes |
WO2017143412A1 (fr) * | 2016-02-24 | 2017-08-31 | Universidade Estadual Paulista "Júlioo De Mesquita Filho" - Unesp | Système folliculaire pour la maturation in vitro d'ovocytes et kit |
CN105624100A (zh) * | 2016-04-06 | 2016-06-01 | 云南中科胚胎工程生物技术有限公司 | 水牛卵母细胞体外成熟培养方法 |
CN113249304B (zh) * | 2021-06-04 | 2022-12-27 | 中国农业科学院北京畜牧兽医研究所 | 萝卜硫素在制备牛卵母细胞体外成熟液中的应用 |
-
1990
- 1990-04-24 IL IL94184A patent/IL94184A0/xx unknown
- 1990-04-27 WO PCT/US1990/002365 patent/WO1990013627A1/fr unknown
- 1990-04-27 AU AU55310/90A patent/AU5531090A/en not_active Abandoned
- 1990-04-30 CA CA002015707A patent/CA2015707A1/fr not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU5531090A (en) | 1990-11-29 |
IL94184A0 (en) | 1991-01-31 |
WO1990013627A1 (fr) | 1990-11-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |