CA2000604A1 - Methode pour activer des proteines biologiquement actives, produites par des techniques genetiques et exprimes chez les procaryotes - Google Patents

Methode pour activer des proteines biologiquement actives, produites par des techniques genetiques et exprimes chez les procaryotes

Info

Publication number
CA2000604A1
CA2000604A1 CA2000604A CA2000604A CA2000604A1 CA 2000604 A1 CA2000604 A1 CA 2000604A1 CA 2000604 A CA2000604 A CA 2000604A CA 2000604 A CA2000604 A CA 2000604A CA 2000604 A1 CA2000604 A1 CA 2000604A1
Authority
CA
Canada
Prior art keywords
prokaryotes
activation
proteins expressed
active proteins
produced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2000604A
Other languages
English (en)
Other versions
CA2000604C (fr
Inventor
Rainer Rudolph
Johannes Buchner
Helmut Lenz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of CA2000604A1 publication Critical patent/CA2000604A1/fr
Application granted granted Critical
Publication of CA2000604C publication Critical patent/CA2000604C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/867—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody produced via recombinant dna technology

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
CA002000604A 1988-10-17 1989-10-13 Methode pour activer des proteines biologiquement actives, produites par des techniques genetiques et exprimes chez les procaryotes Expired - Lifetime CA2000604C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3835350A DE3835350A1 (de) 1988-10-17 1988-10-17 Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DEP3835350.4 1988-10-17

Publications (2)

Publication Number Publication Date
CA2000604A1 true CA2000604A1 (fr) 1990-04-17
CA2000604C CA2000604C (fr) 1999-07-06

Family

ID=6365312

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002000604A Expired - Lifetime CA2000604C (fr) 1988-10-17 1989-10-13 Methode pour activer des proteines biologiquement actives, produites par des techniques genetiques et exprimes chez les procaryotes

Country Status (12)

Country Link
US (1) US5077392A (fr)
EP (1) EP0364926B1 (fr)
JP (1) JPH0793879B2 (fr)
KR (1) KR920010873B1 (fr)
AT (1) ATE101650T1 (fr)
AU (1) AU609645B2 (fr)
CA (1) CA2000604C (fr)
DD (1) DD285613A5 (fr)
DE (2) DE3835350A1 (fr)
DK (1) DK175439B1 (fr)
ES (1) ES2061876T3 (fr)
IL (1) IL92012A (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
US5676947A (en) * 1989-02-07 1997-10-14 Boehringer Manneheim Gmbh Method for treating thromboembolic conditions using thrombolytically active proteins
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4105480A1 (de) * 1991-02-21 1992-08-27 Boehringer Mannheim Gmbh Verbesserte aktivierung von rekombinanten proteinen
WO1993019084A1 (fr) * 1992-03-24 1993-09-30 Synergen, Inc. Repliement et purification de facteurs de croissance i ressemblant a l'insuline
ES2199959T3 (es) * 1993-02-04 2004-03-01 Borean Pharma A/S Procedimiento mejorado para el replegamiento de proteinas.
US6084076A (en) * 1995-12-21 2000-07-04 Ajinomoto Co., Inc. Method of refolding human activin A
WO1997038123A1 (fr) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Procedes de production de proteines eucaryotes, solubles, actives sur plan biologique et contenant des liaisons disulfure, a l'interieur de cellules bacteriennes
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
AU747883B2 (en) 1997-08-15 2002-05-30 Chugai Seiyaku Kabushiki Kaisha Preventives and/or remedies for systemic lupus erythematosus containing anti-IL-6 receptor antibody as the active ingredient
ATE349460T1 (de) * 1998-07-09 2007-01-15 Barofold Inc Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck
EP1077263A1 (fr) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Procédé de préparation des protéines secrétées sous une forme pliée naturelle par co-sécrétion de chaperones
WO2001019970A2 (fr) * 1999-09-15 2001-03-22 Eli Lilly And Company Trypsine exempte de chymotrypsine
US6808902B1 (en) * 1999-11-12 2004-10-26 Amgen Inc. Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules
JP2004530651A (ja) 2000-10-31 2004-10-07 ザ リージェンツ オブ ザ ユニバーシティ オブ コロラド， ア ボディー コーポレイト 高圧を使用する、改善されたタンパク質脱凝集およびリフォールディング
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
BRPI0621124A2 (pt) * 2005-12-22 2011-11-29 Genentech Inc métodos para recuperação de uma proteìna de ligação à heparina
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
WO2008008975A2 (fr) 2006-07-14 2008-01-17 Genentech, Inc. Repliement de protéines recombinantes
CA2663416A1 (fr) * 2006-09-15 2008-03-20 Barofold, Inc. Traitement de proteines sous haute pression permettant de reduire leur immunogenicite
EA022821B1 (ru) 2010-03-17 2016-03-31 Ратиофарм Гмбх Способ получения биологически активного рекомбинантного г-ксф человека
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
WO2013168178A1 (fr) * 2012-03-30 2013-11-14 Krishnan Archana Rajesh Procédé pour la renaturation de polypeptides
US20150376228A1 (en) * 2013-02-22 2015-12-31 Biogenomics Limited Process for high efficiency refolding of recombinant proteins
WO2014167574A1 (fr) * 2013-03-22 2014-10-16 Biogenomics Limited Procédé pour l'isolation et la stabilisation d'intermédiaires clés pour un repliement à haute efficacité de protéines recombinantes
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
CN110643595A (zh) * 2019-10-21 2020-01-03 黑龙江精益检测有限公司 一种对化学修饰后磁性固定化pla1再活化的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GR79124B (fr) * 1982-12-22 1984-10-02 Genentech Inc
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4634586A (en) * 1984-05-21 1987-01-06 The Board Of Trustees Of The Leland Stanford Junior University Reagent and method for radioimaging leukocytes
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
ZW14486A1 (en) * 1985-07-29 1986-10-22 Smithkline Beckman Corp Pharmaceutical dosage unit
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2594845B1 (fr) * 1986-02-21 1989-12-01 Genetica Preparation par voie microbiologique de l'activateur tissulaire humain du plasminogene (t-pa) et conversion de l'enzyme ainsi obtenue en sa forme active
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen

Also Published As

Publication number Publication date
DE58906984D1 (de) 1994-03-24
DK175439B1 (da) 2004-10-18
JPH02227090A (ja) 1990-09-10
KR920010873B1 (ko) 1992-12-19
ES2061876T3 (es) 1994-12-16
EP0364926A3 (fr) 1991-05-02
EP0364926B1 (fr) 1994-02-16
EP0364926A2 (fr) 1990-04-25
DK515989D0 (da) 1989-10-17
US5077392A (en) 1991-12-31
AU4293189A (en) 1990-04-26
CA2000604C (fr) 1999-07-06
IL92012A (en) 1994-12-29
JPH0793879B2 (ja) 1995-10-11
IL92012A0 (en) 1990-07-12
DD285613A5 (de) 1990-12-19
DE3835350A1 (de) 1990-04-19
DK515989A (da) 1990-04-18
KR900006515A (ko) 1990-05-08
AU609645B2 (en) 1991-05-02
ATE101650T1 (de) 1994-03-15

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