CA1340597C - Vecteurs d'expression renfermant le promoteur pl et site de restriction synthetique facilitant le remplacement du site de liaison aux ribosomes; plasmides contenant les vecteurs, hotes contenant les plasmides et methodes connexes - Google Patents

Vecteurs d'expression renfermant le promoteur pl et site de restriction synthetique facilitant le remplacement du site de liaison aux ribosomes; plasmides contenant les vecteurs, hotes contenant les plasmides et methodes connexes

Info

Publication number
CA1340597C
CA1340597C CA000488832A CA488832A CA1340597C CA 1340597 C CA1340597 C CA 1340597C CA 000488832 A CA000488832 A CA 000488832A CA 488832 A CA488832 A CA 488832A CA 1340597 C CA1340597 C CA 1340597C
Authority
CA
Canada
Prior art keywords
plasmid
analog
host
growth hormone
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA000488832A
Other languages
English (en)
Inventor
Haim Aviv
Daniel Bartfeld
Marian Gorecki
Jacob R. Hartman
Dov Kanner
Avigdor Levanon
Hilla Locker-Giladi
Amos B. Oppenheim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Savient Pharmaceuticals Inc
Original Assignee
Savient Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/644,105 external-priority patent/US4742004A/en
Application filed by Savient Pharmaceuticals Inc filed Critical Savient Pharmaceuticals Inc
Priority to US07/449,125 priority Critical patent/US5162217A/en
Priority to CA000616018A priority patent/CA1340464C/fr
Priority to US07/933,500 priority patent/US5455029A/en
Priority to US07/933,682 priority patent/US5457042A/en
Application granted granted Critical
Publication of CA1340597C publication Critical patent/CA1340597C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0089Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775Apolipopeptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/69Increasing the copy number of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/73Expression systems using phage (lambda) regulatory sequences
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CA000488832A 1984-08-27 1985-08-15 Vecteurs d'expression renfermant le promoteur pl et site de restriction synthetique facilitant le remplacement du site de liaison aux ribosomes; plasmides contenant les vecteurs, hotes contenant les plasmides et methodes connexes Expired - Lifetime CA1340597C (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US07/449,125 US5162217A (en) 1984-08-27 1989-12-08 Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
CA000616018A CA1340464C (fr) 1984-08-27 1991-03-01 Methode de production de superoxyde-dismutase dans une cellule bacterienne
US07/933,500 US5455029A (en) 1984-08-27 1992-08-21 Therapeutic compositions comprising a mixture of human CuZn superoxide dismutase analogs
US07/933,682 US5457042A (en) 1984-08-27 1992-08-21 Sod polypeptide analogs

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US64455184A 1984-08-27 1984-08-27
US64424584A 1984-08-27 1984-08-27
US64467184A 1984-08-27 1984-08-27
US64511984A 1984-08-27 1984-08-27
US644,105 1984-08-27
US644,671 1984-08-27
US645,119 1984-08-27
US644,245 1984-08-27
US06/644,105 US4742004A (en) 1984-08-27 1984-08-27 Method for producing enzymatically active eucaryotic sod in bacteria
US644,551 1984-08-27

Publications (1)

Publication Number Publication Date
CA1340597C true CA1340597C (fr) 1999-06-22

Family

ID=27542038

Family Applications (2)

Application Number Title Priority Date Filing Date
CA000488832A Expired - Lifetime CA1340597C (fr) 1984-08-27 1985-08-15 Vecteurs d'expression renfermant le promoteur pl et site de restriction synthetique facilitant le remplacement du site de liaison aux ribosomes; plasmides contenant les vecteurs, hotes contenant les plasmides et methodes connexes
CA000616018A Expired - Lifetime CA1340464C (fr) 1984-08-27 1991-03-01 Methode de production de superoxyde-dismutase dans une cellule bacterienne

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA000616018A Expired - Lifetime CA1340464C (fr) 1984-08-27 1991-03-01 Methode de production de superoxyde-dismutase dans une cellule bacterienne

Country Status (15)

Country Link
EP (2) EP0173280B1 (fr)
JP (4) JP2933280B2 (fr)
AT (2) ATE184916T1 (fr)
AU (1) AU603331B2 (fr)
CA (2) CA1340597C (fr)
DE (2) DE3586473T2 (fr)
DK (1) DK384185A (fr)
GR (1) GR852030B (fr)
HK (1) HK151695A (fr)
HU (2) HU215160B (fr)
IE (1) IE61141B1 (fr)
IL (3) IL76192A (fr)
MY (1) MY130321A (fr)
NZ (1) NZ213154A (fr)
PT (1) PT81025B (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL71689A0 (en) * 1983-04-29 1984-07-31 Yeda Res & Dev Human superoxide dismutase production
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio- Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
EP0340805B1 (fr) * 1983-10-03 1997-07-16 Chiron Corporation Superoxyde dismutase et son expression dans les microorganismes
CA1267615A (fr) * 1984-08-27 1990-04-10 Dan Hadary Methode pour la recuperation d'hormones de croissance purifiees a patir de microorganismes modifies par des techniques de genie genetique
JP2594535B2 (ja) * 1984-11-30 1997-03-26 サントリー株式会社 高発現ベクターおよびその利用
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
EP0239631A4 (fr) * 1985-10-04 1989-01-12 Biotech Res Partners Ltd Apolipoproteines recombinantes et procedes.
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
IL96136A (en) * 1989-11-07 2000-12-06 Bio Technology General Corp Use of hydrophobic chromatography in a method for purification of copper-zinc superoxide dismutase from cells containing same
WO1991007496A1 (fr) * 1989-11-09 1991-05-30 Allied-Signal Inc. Clonage et expression de genes codant pour des polypeptides comprenant une ou plusieurs sequences d'acides amines se repetant
DE4239877C1 (de) * 1992-11-27 1994-03-17 Boehringer Ingelheim Int Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung
GB9303988D0 (en) * 1993-02-26 1993-04-14 Health Lab Service Board Bi-functional expression systems
US6030810A (en) 1997-02-26 2000-02-29 Delgado; Stephen Gregory Cloned tetrodotoxin-sensitive sodium channel α-subunit and a splice variant thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK143412C (da) * 1979-05-17 1982-05-24 Forenede Bryggerier As Fremgangsmaade til udvinding af cu,zn-superoxid-dismutase fra gaer
EP0041767B1 (fr) * 1980-06-06 1993-03-03 Biogen, Inc. Vecteurs améliorés, méthodes de construction de tels vecteurs et d'expression de gènes clonés
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
CA1341012C (fr) * 1982-09-27 2000-06-06 Biogen, Inc. Sequences d'adn, molecules d'adn recombinant, ainsi que des procedes de synthese de polypeptides aux proprietes analogues a l'hormone de croissance porcine
WO1984002918A1 (fr) * 1983-01-21 1984-08-02 Transgene Sa NOUVEAUX VECTEURS D'EXPRESSION ET LEUR APPLICATION A LA PREPARATION D'UNE PROTEINE AYANT L'ACTIVITE DE L'ANTITRYPSINE-alpha1 HUMAINE
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio- Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
EP0138111B1 (fr) * 1983-10-03 1991-11-21 Chiron Corporation Clonage de la dismutase du peroxyde et expression dans des micro-organismes

Also Published As

Publication number Publication date
JPH08283296A (ja) 1996-10-29
GR852030B (fr) 1985-12-30
HK151695A (en) 1995-09-29
IE852093L (en) 1986-02-27
PT81025B (pt) 1988-01-22
IE61141B1 (en) 1994-10-05
DE3586473D1 (de) 1992-09-17
IL110039A (en) 1996-12-05
IL108976A0 (en) 1994-06-24
EP0173280A1 (fr) 1986-03-05
HU215160B (hu) 1998-10-28
DE3586473T2 (de) 1993-02-04
IL110039A0 (en) 1994-10-07
JP2777074B2 (ja) 1998-07-16
CA1340464C (fr) 1999-03-23
EP0483113A3 (en) 1992-05-13
DK384185A (da) 1986-02-28
AU4667785A (en) 1986-03-06
PT81025A (en) 1985-09-01
HU214977B (hu) 1998-08-28
DK384185D0 (da) 1985-08-23
DE3588216T2 (de) 2000-02-03
IL76192A0 (en) 1985-12-31
JP2933280B2 (ja) 1999-08-09
HUT40697A (en) 1987-01-28
AU603331B2 (en) 1990-11-15
IL76192A (en) 1996-12-05
JPS61111693A (ja) 1986-05-29
MY130321A (en) 2007-06-29
EP0483113A2 (fr) 1992-04-29
IL108976A (en) 1996-12-05
ATE79407T1 (de) 1992-08-15
EP0483113B1 (fr) 1999-09-22
JP2688180B2 (ja) 1997-12-08
NZ213154A (en) 1988-11-29
DE3588216D1 (de) 1999-10-28
JPH07236490A (ja) 1995-09-12
ATE184916T1 (de) 1999-10-15
JPH07222596A (ja) 1995-08-22
EP0173280B1 (fr) 1992-08-12

Similar Documents

Publication Publication Date Title
US5457042A (en) Sod polypeptide analogs
US6030611A (en) Therapeutic SOD compositions and uses thereof
US5143836A (en) Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
AU671728B2 (en) Methods of production of analogues of human Cu/Zn superoxide dismutase
EP0131843B1 (fr) Vecteurs d'expression pour la production accrue de polypeptides, plasmides contenant ces vecteurs, hôtes contenant ces plasmides, produits manufacturés par ces vecteurs et méthodes correspondantes
CA1340597C (fr) Vecteurs d'expression renfermant le promoteur pl et site de restriction synthetique facilitant le remplacement du site de liaison aux ribosomes; plasmides contenant les vecteurs, hotes contenant les plasmides et methodes connexes
JP2609449B2 (ja) ヒトマンガンスーパーオキシドジスムターゼ▲下c▼DNA、その細菌中での発現及び酵素活性ヒトマンガンスーパーオキシドジスムターゼの回収方法
US5256546A (en) Bacterial expression of porcine growth hormone
US5637495A (en) Plasmids for production of human growth hormone or polypeptide analog thereof, hosts containing the plasmids, products manufactured thereby, and related methods
US5527691A (en) Expression vectors containing lambdapl promoter and T1 T2 rRNA termination sequence, plasmids containing the vectors, hosts containing the plasmids and related methods
US5670371A (en) Bacterial expression of superoxide dismutase
US5759816A (en) Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
US5198361A (en) Plasmids for production of met-asp-gln bovine growth hormone, hosts containing the plasmids, products manufactured thereby and related methods
US5112744A (en) Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase

Legal Events

Date Code Title Description
MKEX Expiry

Effective date: 20160622