CA1340063C - Production of animal lysozyme c via secretion from pichia pastoris and composition therefor - Google Patents

Production of animal lysozyme c via secretion from pichia pastoris and composition therefor

Info

Publication number
CA1340063C
CA1340063C CA000581993A CA581993A CA1340063C CA 1340063 C CA1340063 C CA 1340063C CA 000581993 A CA000581993 A CA 000581993A CA 581993 A CA581993 A CA 581993A CA 1340063 C CA1340063 C CA 1340063C
Authority
CA
Canada
Prior art keywords
lysozyme
pastoris
dna
gene
segment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA000581993A
Other languages
English (en)
French (fr)
Inventor
Mary Ellen Digan
Michael Miller Harpold
Stephen Vernon Lair
Gregory Patrick Thill
Robert Steven Siegel
Steven Bradley Ellis
Mark Edward Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
SIBIA Neurosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SIBIA Neurosciences Inc filed Critical SIBIA Neurosciences Inc
Application granted granted Critical
Publication of CA1340063C publication Critical patent/CA1340063C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02—Acyclic radicals, not substituted by cyclic structures
    • C07H15/12—Acyclic radicals, not substituted by cyclic structures attached to a nitrogen atom of the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA000581993A 1987-11-02 1988-11-02 Production of animal lysozyme c via secretion from pichia pastoris and composition therefor Expired - Fee Related CA1340063C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US115,940 1980-01-28
US11594087A 1987-11-02 1987-11-02

Publications (1)

Publication Number Publication Date
CA1340063C true CA1340063C (en) 1998-09-29

Family

ID=22364276

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000581993A Expired - Fee Related CA1340063C (en) 1987-11-02 1988-11-02 Production of animal lysozyme c via secretion from pichia pastoris and composition therefor

Country Status (8)

Country Link
EP (1) EP0343231A4 (ja)
JP (1) JPH02501976A (ja)
KR (1) KR890701606A (ja)
AU (1) AU2825489A (ja)
CA (1) CA1340063C (ja)
DK (1) DK327589A (ja)
FI (1) FI893207A0 (ja)
WO (1) WO1989004320A1 (ja)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04501662A (ja) * 1988-09-26 1992-03-26 ザ・サルク・インスティチュート・バイオテクノロジー／インダストリアル・アソシエイツ・インコーポレーテッド 混合給送組換え酵母発酵
US5122465A (en) * 1989-06-12 1992-06-16 Phillips Petroleum Company Strains of pichia pastoris created by interlocus recombination
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
US5850025A (en) * 1991-09-19 1998-12-15 Sibia Neurosciences, Inc. Protection of plants against plant pathogens
US5422108A (en) * 1991-09-19 1995-06-06 Smart Plants International Inc. Protection of plants against plant pathogens
CN109810963B (zh) * 2017-11-22 2021-09-28 上海复华兴生物技术有限公司 一种新的牛肠源溶菌酶蛋白的dna序列及其生产工艺和应用
CN110903991A (zh) * 2019-11-13 2020-03-24 浙江新银象生物工程有限公司 一种含高拷贝数人源溶菌酶基因的重组毕赤酵母工程菌及其应用
CN112029783B (zh) * 2020-09-15 2022-05-27 西南民族大学 利用毕赤酵母异源表达反刍动物胃溶菌酶的方法
CN115873733B (zh) * 2022-07-20 2024-06-25 青岛蔚蓝生物集团有限公司 一种高产溶菌酶的毕赤酵母菌株及其应用

Also Published As

Publication number Publication date
WO1989004320A1 (en) 1989-05-18
KR890701606A (ko) 1989-12-21
FI893207A (fi) 1989-06-30
JPH02501976A (ja) 1990-07-05
AU2825489A (en) 1989-06-01
FI893207A0 (fi) 1989-06-30
EP0343231A4 (en) 1990-09-05
DK327589D0 (da) 1989-06-30
DK327589A (da) 1989-06-30
EP0343231A1 (en) 1989-11-29

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Legal Events

Date Code Title Description
MKLA Lapsed

Effective date: 20150929