CA1326017C - Ribofuranuronic acid derivatives - Google Patents
Ribofuranuronic acid derivativesInfo
- Publication number
- CA1326017C CA1326017C CA000563261A CA563261A CA1326017C CA 1326017 C CA1326017 C CA 1326017C CA 000563261 A CA000563261 A CA 000563261A CA 563261 A CA563261 A CA 563261A CA 1326017 C CA1326017 C CA 1326017C
- Authority
- CA
- Canada
- Prior art keywords
- desoxy
- purinyl
- beta
- methyl
- ribofuranuronic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
Abstract
ABSTRACT
In position 2 substituted 1'-desoxy-1'-(6-amino-9-puri-nyl)-.beta.-D-ribofuranuronic acid amides and thioamides of formula
In position 2 substituted 1'-desoxy-1'-(6-amino-9-puri-nyl)-.beta.-D-ribofuranuronic acid amides and thioamides of formula
Description
SANDOZ LTD. CASE 100-7069 BASLL
NBU RIBOYURANURONIC ACID DERIVATIVES
The invention relates to new in position 2 substituted l'-desoxy-1'-(6-amino-9-purinyl)-~-D-ribofuranuronic acid amides and thioamides, process for their production and their use in the treatment of e.g. raised blood pressure, The new, in posltion 2 substituted 1'-desoxy-1'-(6-amlno-9-purinyl)-~-D-ribofuranuronic acid amides and thioamides pro-duced according to the invention are referred to herelnafter as compounds accordlng to the invention.
The compounds may be substituted where desired e.g. in free amino groups.
The invention especially provides in position 2 substituted l'-desoxy-1'-(6-amino-9-purinyl)-~-D-ribofuranuronic acid amides and thioamides of formula I
NBU RIBOYURANURONIC ACID DERIVATIVES
The invention relates to new in position 2 substituted l'-desoxy-1'-(6-amino-9-purinyl)-~-D-ribofuranuronic acid amides and thioamides, process for their production and their use in the treatment of e.g. raised blood pressure, The new, in posltion 2 substituted 1'-desoxy-1'-(6-amlno-9-purinyl)-~-D-ribofuranuronic acid amides and thioamides pro-duced according to the invention are referred to herelnafter as compounds accordlng to the invention.
The compounds may be substituted where desired e.g. in free amino groups.
The invention especially provides in position 2 substituted l'-desoxy-1'-(6-amino-9-purinyl)-~-D-ribofuranuronic acid amides and thioamides of formula I
- 2 ~ l ~2 6 o ~a~e 100-7069 2\
R
R
wherein R1 signifies (Cl_6)alkyl which may be optionally monosubstituted by a hydroxyl, a -SH- or a -N /
group, wherein R4 and R5 signify independently from each other hydrogen or (C~_4)alkyl; (C3_7)-alkenyl, (C3_7)al-kinyl, (C3 ~)cycloalkyl which may be optionally mono- or ~ R4 i-substitueed by a hydroxyl, a -SH- or a -N group, wherein R4 and R5 are defined as above;
(C3_7)cycloalkyl(Cl_3)alkyl which may be optionally mono-or di-substituted in the cycloalkyl ring by a hydroxyl, a -SH- or a -N R5 group, wherein R4 and R5 are de fined as above; phenyl which may be optionally mono- or di-substituted by halogen with an atomic number of 9-35, (C1_4)alkyl, (C~_4)alkoxy, a hydroxyl, a -SH- a -S-(C1_4)alkyl, a -502-(C~_4)alkyl, a trifluoromethyl- or a ~ 1 3260 1 7 - 3 - Case 100-7069 -S02-N ~ group, wherein R9 and R5 are defined as above; phenyl-(C1_6)alkyl which is optionally mono- or di-substituted in the phenyl ring by halogen with an atomic number of 9-35, tC1_4)alkyl, (C1_4)alkoxy, a hydroxyl, a -SH-, a -S-(Cl_4)alkyl-, a -S02(Cl_4)alkyl- or a -S02-N - group, wherein R4 and R5 are defined as ~R5 above, and the (Cl_6)alkylene chain is straight-chain or branched and may be optionally substituted by a hydroxyl group; phenyl-(C3_~)alkenyl- which may be optionally substituted in the phenyl rin~ by halogen with an atomic number of 9-35, (Cl_4)alkyl, (C1_4)alkoxy, a hydroxyl, a -SH-, a -S-(C1_4)alkyl-, a -S02-(Cl_4)alkyl- or a ,R4 -S02-N ~ group, wherein R4 and Rs are defined as above; a 5 or 6 membered, monocyclic heteroaryl whlch contains one or two nitrogen atoms or one oxygen atom or one sulphur atom and respectively one nitrogen atom; or a 5 or 6 membered, monocyclic heteroaryl-(C1_s)alkyl containing in the heteroaryl moiety one or two nitrogen atoms or one oxygen atom or one sulphur atom and respectively one nitrogen atom, whereby the alkylene moiety is straight-chain or branched and may be optionally sub~tituted by a hydroxyl group, and R~ signifies hydrogen, (CL 4 )alkyl which may be optionally mono-substituted by a hydroxyl, a -S8- or a -N ~
Rs ~` 1326017 - 4 - Case 100-7069 group, wherein R4 and R5 are defined as above, or it signifies (C3_8)cycloalkyl, and R3 is hydrogen or (Cl_4)alkyl which may be optionally ,R4 mono-substituted by a hydroxyl, a -SH- or a -N
`~R5 group, wherein R4 and R5 are defined as above, and R6 is halogen, (Cl_4)alkyl, (C3_5)cycloalkyl, cyano, or it denotes groups of formulae ~ORq~ -SX4, -N
wherein R4 and Rs are defined as above and X signifies = O or = S.
Of the compounds of formula I, preferred compounds possess the formula Ia , HH
R 6a J~ N~ l a ~/ NJ~ y R3 1--~
HO OH
wherein Rla S ignifies (C1_ 6) alkyl, (C3_7) cycloalkyl, which may be optionally mono- or di-substituted by a hydroxyl, a A
~ ~" t326017 - 5 - Case 100-7069 -SH- or a -N group, wherein R4 and R5 are defined Rs as above; phenyl-(C1_6)alkyl which may be mono- or di-substituted in the phenyl ring by halogen with an atomic number of 9-35, (Cl_4)alkyl, (Cl_4)alkoxy, a hydroxyl, a-SH-, a -S-(Cl_4)alkyl, a -S02-(Cl_4)alkyl or a ,R4 -S02-N\ group, wherein R4 and R5 are defined as above, whereby the (Cl_6)alkylene chain is straight-chain or branched and may be optionally substituted by a hydroxyl group; or phenyl which may be optionally mono- or di-substituted by halogen ~ith an atomic number of 9-35, (C1_4)alkyl, (Cl_4)alkoxy, a hydroxyl, a -SH-, a -S-(C1_4)alkyl, a -S02-(Cl_4)alkyl- a trifluoromethyl_ or ~ R4 a -S02-N \ group, wherein R4 and R5 are defined R~
as above, R2~ is hydrogen, (Cl_4)alkyl which may be optionally mono-substituted by a hydroxyl, a -SH- or a -N
\ R5 group, wherein R~ and R5 are defined as above; or ( C3 _ 6 ) cycloalkyl, and R3~ is hydrogen or (Cl_4)alkyl which may be optionally mono-substituted by a hydroxyl, a -SH- or a -N ~
Rs -` ~3260~7 - 6 - Case 100-7069 group, wherein R4 and Rs are defined as above9 and R6a is halogen with an atomic number of 9-35, (C1_4)alkyl or ~ R4 it denotes groups of formulae -OR4, -SR4 or -N
wherein R4 and Rs are defined as above and X denotes , O or = S.
Of the compounds of formula I, especially preferred compounds possesS the formula Ib, ~Rl b b/~;
HO OH
wherein R1b signifies hydrogen, (Cl_6)alkyl, (C3_~)cycloalkyl which may be optionally mono- or di-substituted by a hydroxyl, ~ R4 -SH- or a -N group, wherein R4 and Rs are defined as above; or phenyl which may be optionally mono-or di-substituted by halogen with an atomic number of 9-35, (Cl_~alkyl, a (Cl_ 4) alkoxy, a hydroxyl, a -SH-, a S-(Cl_4)alkyl, a-SO~-(C1_4)alkyl- a trifluoromethyl- or a ~` ` 1326017 - 7 - Case 100-7069 -S02-N group, wherein R4 and R5 are defined as ~ R5 above, R2b is hydrogen, (Cl_4)alkyl which may be optionally ~ R4 mono-substituted by a hydroxyl, a -SH- or a -N
\ Rs group, wherein R4 and R5 are defined as above; or (C3_6)cycloalkyl and R3b is (C1_4)alkyl, and R6b i9 (Cl_~)alkyl, chlorine, bromine, methoxy, methylthio, methylamino or dimethylamino and X denotes , 0 or ,S.
In formula I, halogen with an atomic number of 9-35 denotes fluorine, chlorine or bromine, preferably chlorine, a (Cl_~)alkyl group is methyl, ethyl, n-propyl, i propyl, n-butyl, i-butyl, tert.-butyl, and if it contains up to 6 carbon atoms, it is also n-pentyl, i-pentyl, 3-pentyl, n-hexyl, i-hexyl, etc.especially methyl, ethyl, isopropyl or 3-pentyl, a (Cl_4)alkoxy group is methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, i-butoxy, tert.-butoxy, and if it contains up to 6 carbon atoms, it is also n-pentoxy, i-pentoxy, n-hexoxy, i-hexoxy, etc., especially me-thoxy, (C3_7)alkenyl is methallyl, butenyl, pentenyl, etc., whereby the chain may be straight or branched and the double bond may be found in various positions, but not ad~acent to nitrogen, (C3_7)alkinyl is propinyl, butinyl, pentinyl, hexinyl, wherby the chain is straight or branched and the triple bond may be - 8 - Case 100-7069 found in various positions, but not adjacent to nitrogen. (3_7)-cycloalkyl signifies cyclopropyl, cyclobutyl, cyclopentyl, cyclo-hexyl or cyclohep~yl especially cyclopentyl. If it is substi-tuted, the substituents are respectively in o-, p- or m-position, but appropriately either when disubstituted in o-, o'-position, or when monosubsituted in p-position. (C3_7)cycloalkyl(Cl_3)alkyl may denote the above-mentioned cycloalkyl and alkyl radicals, whereby as shown, the substituents may be bonded. Substitution of the phenyl ring may take place in o-, m- or p-position, whereby when disubstituted this is preferably in m- and p-position, and when monosubstituted this is in m or p position. In phenylalkyl, the alkyl radicals and the substitution of the phenyl ring are as discussed above.
The compounds according to the invention are obtained e.g.
by cleavage of an isopropylidene group from in position 2 substituted 1'-desoxy-1'-(6-amino-9-purinyl)-2'.3'-isopro-pylidene-~_D-ribofuranuronic acid amides and thioamides e.g. of formula II R
HN~ 1 R ~X
R2 \ N
wherein Rl, R2, R3, R6 and X have the definitions given above.
The above process conveniently takes place by treating compounds o formula II with an agent which cleaves the isopropylidene group. Trifluoroacetic acid has proved to be ,.
group, wherein R4 and R5 signify independently from each other hydrogen or (C~_4)alkyl; (C3_7)-alkenyl, (C3_7)al-kinyl, (C3 ~)cycloalkyl which may be optionally mono- or ~ R4 i-substitueed by a hydroxyl, a -SH- or a -N group, wherein R4 and R5 are defined as above;
(C3_7)cycloalkyl(Cl_3)alkyl which may be optionally mono-or di-substituted in the cycloalkyl ring by a hydroxyl, a -SH- or a -N R5 group, wherein R4 and R5 are de fined as above; phenyl which may be optionally mono- or di-substituted by halogen with an atomic number of 9-35, (C1_4)alkyl, (C~_4)alkoxy, a hydroxyl, a -SH- a -S-(C1_4)alkyl, a -502-(C~_4)alkyl, a trifluoromethyl- or a ~ 1 3260 1 7 - 3 - Case 100-7069 -S02-N ~ group, wherein R9 and R5 are defined as above; phenyl-(C1_6)alkyl which is optionally mono- or di-substituted in the phenyl ring by halogen with an atomic number of 9-35, tC1_4)alkyl, (C1_4)alkoxy, a hydroxyl, a -SH-, a -S-(Cl_4)alkyl-, a -S02(Cl_4)alkyl- or a -S02-N - group, wherein R4 and R5 are defined as ~R5 above, and the (Cl_6)alkylene chain is straight-chain or branched and may be optionally substituted by a hydroxyl group; phenyl-(C3_~)alkenyl- which may be optionally substituted in the phenyl rin~ by halogen with an atomic number of 9-35, (Cl_4)alkyl, (C1_4)alkoxy, a hydroxyl, a -SH-, a -S-(C1_4)alkyl-, a -S02-(Cl_4)alkyl- or a ,R4 -S02-N ~ group, wherein R4 and Rs are defined as above; a 5 or 6 membered, monocyclic heteroaryl whlch contains one or two nitrogen atoms or one oxygen atom or one sulphur atom and respectively one nitrogen atom; or a 5 or 6 membered, monocyclic heteroaryl-(C1_s)alkyl containing in the heteroaryl moiety one or two nitrogen atoms or one oxygen atom or one sulphur atom and respectively one nitrogen atom, whereby the alkylene moiety is straight-chain or branched and may be optionally sub~tituted by a hydroxyl group, and R~ signifies hydrogen, (CL 4 )alkyl which may be optionally mono-substituted by a hydroxyl, a -S8- or a -N ~
Rs ~` 1326017 - 4 - Case 100-7069 group, wherein R4 and R5 are defined as above, or it signifies (C3_8)cycloalkyl, and R3 is hydrogen or (Cl_4)alkyl which may be optionally ,R4 mono-substituted by a hydroxyl, a -SH- or a -N
`~R5 group, wherein R4 and R5 are defined as above, and R6 is halogen, (Cl_4)alkyl, (C3_5)cycloalkyl, cyano, or it denotes groups of formulae ~ORq~ -SX4, -N
wherein R4 and Rs are defined as above and X signifies = O or = S.
Of the compounds of formula I, preferred compounds possess the formula Ia , HH
R 6a J~ N~ l a ~/ NJ~ y R3 1--~
HO OH
wherein Rla S ignifies (C1_ 6) alkyl, (C3_7) cycloalkyl, which may be optionally mono- or di-substituted by a hydroxyl, a A
~ ~" t326017 - 5 - Case 100-7069 -SH- or a -N group, wherein R4 and R5 are defined Rs as above; phenyl-(C1_6)alkyl which may be mono- or di-substituted in the phenyl ring by halogen with an atomic number of 9-35, (Cl_4)alkyl, (Cl_4)alkoxy, a hydroxyl, a-SH-, a -S-(Cl_4)alkyl, a -S02-(Cl_4)alkyl or a ,R4 -S02-N\ group, wherein R4 and R5 are defined as above, whereby the (Cl_6)alkylene chain is straight-chain or branched and may be optionally substituted by a hydroxyl group; or phenyl which may be optionally mono- or di-substituted by halogen ~ith an atomic number of 9-35, (C1_4)alkyl, (Cl_4)alkoxy, a hydroxyl, a -SH-, a -S-(C1_4)alkyl, a -S02-(Cl_4)alkyl- a trifluoromethyl_ or ~ R4 a -S02-N \ group, wherein R4 and R5 are defined R~
as above, R2~ is hydrogen, (Cl_4)alkyl which may be optionally mono-substituted by a hydroxyl, a -SH- or a -N
\ R5 group, wherein R~ and R5 are defined as above; or ( C3 _ 6 ) cycloalkyl, and R3~ is hydrogen or (Cl_4)alkyl which may be optionally mono-substituted by a hydroxyl, a -SH- or a -N ~
Rs -` ~3260~7 - 6 - Case 100-7069 group, wherein R4 and Rs are defined as above9 and R6a is halogen with an atomic number of 9-35, (C1_4)alkyl or ~ R4 it denotes groups of formulae -OR4, -SR4 or -N
wherein R4 and Rs are defined as above and X denotes , O or = S.
Of the compounds of formula I, especially preferred compounds possesS the formula Ib, ~Rl b b/~;
HO OH
wherein R1b signifies hydrogen, (Cl_6)alkyl, (C3_~)cycloalkyl which may be optionally mono- or di-substituted by a hydroxyl, ~ R4 -SH- or a -N group, wherein R4 and Rs are defined as above; or phenyl which may be optionally mono-or di-substituted by halogen with an atomic number of 9-35, (Cl_~alkyl, a (Cl_ 4) alkoxy, a hydroxyl, a -SH-, a S-(Cl_4)alkyl, a-SO~-(C1_4)alkyl- a trifluoromethyl- or a ~` ` 1326017 - 7 - Case 100-7069 -S02-N group, wherein R4 and R5 are defined as ~ R5 above, R2b is hydrogen, (Cl_4)alkyl which may be optionally ~ R4 mono-substituted by a hydroxyl, a -SH- or a -N
\ Rs group, wherein R4 and R5 are defined as above; or (C3_6)cycloalkyl and R3b is (C1_4)alkyl, and R6b i9 (Cl_~)alkyl, chlorine, bromine, methoxy, methylthio, methylamino or dimethylamino and X denotes , 0 or ,S.
In formula I, halogen with an atomic number of 9-35 denotes fluorine, chlorine or bromine, preferably chlorine, a (Cl_~)alkyl group is methyl, ethyl, n-propyl, i propyl, n-butyl, i-butyl, tert.-butyl, and if it contains up to 6 carbon atoms, it is also n-pentyl, i-pentyl, 3-pentyl, n-hexyl, i-hexyl, etc.especially methyl, ethyl, isopropyl or 3-pentyl, a (Cl_4)alkoxy group is methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, i-butoxy, tert.-butoxy, and if it contains up to 6 carbon atoms, it is also n-pentoxy, i-pentoxy, n-hexoxy, i-hexoxy, etc., especially me-thoxy, (C3_7)alkenyl is methallyl, butenyl, pentenyl, etc., whereby the chain may be straight or branched and the double bond may be found in various positions, but not ad~acent to nitrogen, (C3_7)alkinyl is propinyl, butinyl, pentinyl, hexinyl, wherby the chain is straight or branched and the triple bond may be - 8 - Case 100-7069 found in various positions, but not adjacent to nitrogen. (3_7)-cycloalkyl signifies cyclopropyl, cyclobutyl, cyclopentyl, cyclo-hexyl or cyclohep~yl especially cyclopentyl. If it is substi-tuted, the substituents are respectively in o-, p- or m-position, but appropriately either when disubstituted in o-, o'-position, or when monosubsituted in p-position. (C3_7)cycloalkyl(Cl_3)alkyl may denote the above-mentioned cycloalkyl and alkyl radicals, whereby as shown, the substituents may be bonded. Substitution of the phenyl ring may take place in o-, m- or p-position, whereby when disubstituted this is preferably in m- and p-position, and when monosubstituted this is in m or p position. In phenylalkyl, the alkyl radicals and the substitution of the phenyl ring are as discussed above.
The compounds according to the invention are obtained e.g.
by cleavage of an isopropylidene group from in position 2 substituted 1'-desoxy-1'-(6-amino-9-purinyl)-2'.3'-isopro-pylidene-~_D-ribofuranuronic acid amides and thioamides e.g. of formula II R
HN~ 1 R ~X
R2 \ N
wherein Rl, R2, R3, R6 and X have the definitions given above.
The above process conveniently takes place by treating compounds o formula II with an agent which cleaves the isopropylidene group. Trifluoroacetic acid has proved to be ,.
- 9 - Case 100-7069 especially suitable for this. A further cleavable agent is aqueous hydrochloric acid or aqueous formic acid.
The compounds of formula II used as starting compounds are obtained by introducing an isopropyliden protecting group into compounds of formula III, HN~Rl R6 ~ ~ N III
~ \l H
IH IH
wherein Rl is defined as above and Rc' denotes halogen, (C1_4)alkyl or (C3_~)cycloalkyl, (described for example in DE-OS
1 670 175; BRIT. PAT 1 075 008 and JOC (1968) 2583), by reacting it with acetone in the presence of an acid, for example p-toluenesulphonic acid, whereby componds of formula IV
HN,Rl R ~ ~
~ 0 ~
HO ~ ~
o~o wherein R~ and R6' are defined as above, are obtained, then oxidising them in known manner to form compounds of formula V, - 10 - Case 100-7069 N~R~
Ro~ ) V
HOOC~
1~1 wherein Rl and R6' are defined as above, using an oxidation agent, for example pyridinium dichromate, and subsequently converting this in known manner, using a chlorination agent such as thionyl chloride, into the acid chloride of formula VI, R '~N~N~ ~
ClOC~ 'Jl 0><0 wherein Rl and R6' are defined as above, and then reacting this with a compound of formula VII, R2 ~ VII
wherein R2 and R3 have the definitions given above, in known manner, to form compounds of for0ula IIa Case 100-7069 HN ~R1 1`~N~
R 6 o ~ IIa R
(partial structure of compounds of formula II), wherein R1, R2, R3 and R6' are defined as above.
The radical R6"', which is defined as below, is introduced into compounds of formula IIa, wherein R6' is chlorine or bromine, by reacting them with compounds of formula HR6"' wherein R6"' denotes a cyano group or groups of formulae -OR4, ~ R4 SR4 or -N ' , wherein R4 and R~ are defined as above, in ~ R~
a strongly alkaline medium, for example in the presence of sodium, or by reacting them with the corresponding amines in an autoclave at temperatures of above 100C. The compounds of formula IIb H~Rl ~ ~ ~ IIb 2\ N ~ C
` 1326017 - 12 - Case 100-7069 (compounds of formula II, wherein X denotes = O and R6 comprises the significancies of both R6' and R6"') wherein Rl, R2, R3 and R6 are defined as above which are obtained according to the preceding steps are converted by appropriate thianation into compounds of formula IIc Rl L:;~
~6 5 ~ IIc ~6 R3 ~
(compounds of formula II, wherein X denote~ ~ S) wherein Rl, R2, R3 and R6 are defined as above.
The thianation process is suitably effected using known thianation agents, for example hydrogen sulphide, phosphorus pentasulphide or LAUESSON'S REAGENT (p-methoxyphenylthiophosphlne sulphide dimer). The latter reagent i9 preferred. The reaction itself takes place in known manner. If for example hydrogen sulphide is used, an acid ~uch as hydrochloric acid is con-veniently added in catalytic doses, and the reaction is carried out in a polar solvent such as acetic acid or ethanol. Uhen using LAUESSON'S REAGENT, the reaction is conveniently carried out in a dry solvent such as toluene or methylene chloride.
A further method for the production of compounds of formula IIb, wherein R6 is (Cl_4)alkyl, is that 1'-desoxy-1'(2-alkyl-6-hydroxy-9-purinyl)-~-D-ribose of formula IIIa, -` ~ 1326017 - 13 - Case 100-7069 OH
N~ ~) O ¦ IIla HO ~ ) OH OH
wherein R6~V is (C1_4)alkyl, is reacted with acetone in the pre-sence of an acid, for example p-toluenesulphonic acid, to form compounds of formula IVa, OH
R iv ~
6 C~ IVa ~<o wherein R61V is defined as above, then these are oxidised using an oxidatlon agent, for example potassium permanganate, ln an alkaline medium, to produce compounds of formula Va, OH
6 o ~
' ~I Va X
1 3260~ 7 - 14 - Case 100-7069 wherein R61V is defined as above, then these are treated with a chlorination agent, for example phosphorus oxichloride, thus being converted into compounds of formula VIa, Cl R6 i V ol~ ~
C ~ Vla O O
X
wherein R61V is defined as above, then these are reacted with the above-mentioned compounds of formula VII to produce compounds of formula VIIIa, R6~ 0~ ld X
wherein R2, R3 and RCiv are defined as above, and these are converted by reacting with compounds of formula X, Rl-NH2 X
wherein R1 is defined as given above, to form compounds of formula IIb, wherein R6 is (C1_4)alkyl. The compounds of formula IIb thus obtained, wherein R6 is (Cl_4)alkyl, may be converted by - 15 - Case 100-7069 thianation as described above into the corresponding compounds of formula II.
The other above-described reactions take place using knovn methods, for example also uslng the processes described in the examples.
If in the compounds of formula I, Rl denotes groups which re substituted by a -N - group, these compounds can - R~
form salts with strong acids. Preferred salts are the hydrochlorides, hydrobromides or fumarates.
Insofar as the production of the required starting materials is not described, these are known or may be produced by known processes, or analogously to the processes described here, or analogously to known processes.
In the following examples, all temperatures are given in degrees celsius and are uncorrected.
- 16 - Case 100-7069 Example 1 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-~-D-ribofuranuronic acid N-ethylamide 1.4 g of 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2',3'-isopropylidene-~_D-ribofuranuronic acid-N-ethyl-amide are left to stand for 2 hours at 0 and for 1 hour at room temperature in 10 ml of 90% trifluoroacetic acid. The mixture is then totally concentrated under reduced pressure and the residue is par~itioned between ethyl acetate and diluted, aqueous ammonia. After washing with saturated, aqueous sodium chloride solution, the product is dried over sodium sulphate and totally concentrated. The residue is dissolved in a little methanol and the final product is crystallised by adding ethylether. M.p.
195-197.
The 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2~,3~-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide used as the starting material may be produced e.g. as follows:
a) 7.5 g of potassium permanganate are added to a solution of 7.8 g of 1'-desoxy-1'-(2-methyl-6-hydroxy-9-purinyl)-2',3-isopropylidene-~-D-ribose in 120 ml of water and 4.8 ml of lON sodium hydroxide, and the mixture is stirred for 1 hour at 30. Then, 1 g of sodium hydrogen sulphite is added and the colourless solution is filtered over Hyflo after stirring for S minutes. The filtrate is then con-centrated to ca. 30 ml under reduced pressure, and set at pH 4 with concentrated hydrochloric acid at 0. 1'-deso-xy-1'-(2-methyl-6-hydroxy-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid is precipitated in crystalline form. M.p. after washing with acetone and drying: 263 (decomp.).
- 17 - Case 100-7069 b) 3.3 g f 1'-desoxy-1'-(2-methyl-6-hydroxy-9-purinyl)-2',3'-isopropylidene-k-D-ribofuranuronic acid are stirred into 16.8 ml of phosphorus oxychloride for 15 minutes in an oil bath of 85, then mixed with 1.6 ml of N,N-diethyl-aniline and stirred for a further 2 hours at the same temperature. The mixture is subsequently totally con-centrated under reduced pressure and the residue is dissolved in 60 ml of tetrahydrofuran. This solution is cooled to -40 and is mixed with ethylamine until a basic reaction takes place. After 10 minutes, the solution is poured onto ice water and shaken with ethyl acetate. After washing with saturated sodium chloride solution and drying over sodium sulphate, the solution is totally concentrated and the residue is eluted on silica gel with ethyl acetate. The purified l'-desoxy-1'-(2-methyl-6-chloro-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide is a white foam and in ethyl acetate has a Rf value of 0.5.
c) 1.2 g of 1'-tesoxy-1'-(2-methyl-6-chloro-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide and 1.2 ml of cyclopentylamine are stirred into 30 ml of dioxane for 1 hour in an oil bath of 105. After cooling, filtration i9 effected, the filtrate is concentrated and the residue is eluted on 60 g of silica gel with ethyl acetate. The pure l'-desoxy-1'-(2-methyl-6-cyclopentyl-amino-9-purinyl)-2',3'-isopropylidene-0-D-ribofuranuronic acid-N-ethylamide i9 obtained as a white foam. Rf value in ethyl acetate: 0.45.
The following compounds of formula I, in which Rl, R2, R~
and Rc are defined as follows and wherein X is always . 0 are obtained analogously to example 1:
~ 1 32601 7 - 18 - Case 100-7069 Example Rl R2 R3 R6 M.P.
2 p-Methoxyphenyl H Et Me 221-224 3 Cyclopentyl H Et Me 196-198 4 Cyclopentyl H Et Isopropyl amorphous Cyclopentyl H Et Cl 133-135 6 Cyclopentyl H Et Br 188-190 7 Cyclopentyl H Et MeO 226-229 8 Cyclopentyl H Et MeS amorphous 9 Cyclopentyl H Et Me2N amorphous Cyclopentyl H Et MeHN 193-194 11 H H Et Br 240-241 12 p-Ethoxyphenyl H Et Me 120-125 13 3,4-Dimethoxyphenyl H Et Me 236-239 14 3-Pentyl H Et Me 181-183 m-Fluorophenyl H Et Me 137-142 16 p-Fluorophenyl H Et Me 257-259 17 p-Chlorophenyl H Et Me 255-258 18 Isopropyl H Et Me 187-194 19 p-Trifluoromethylphenyl H Et Me 248-250 The l'-desoxy-1'-(2-chloro-6-cyclopentylamino-9-puri-nyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide also useful as the starting material for the preparation of compounds o formula II wherein R6 has the significance of R6"' may be produced e.g. as follows:
a) 8.8 ml of ortho-formic acid trimethylester is added in drops at room temperature to 7.4 g of 1'-desoxy-1'-t2-chloro-6-cyclopentylamino-9-purinyl)-~-D-ribose and 4.2 g of p-tolu-ene-sulphonic acid in 120 ml of acetone. After 3 hours, the - 19 - Case 100-7069 deposit is filtered off and washed with acetone and diethyl-ether. Then, the dried deposit is added in portions whilst stirring to a solution of 3.6 g of sodium hydrogen carbonate in 150 ml of water and 75 ml of ethyl acetate. The organic phase is separated, washed with saturated sodium chloride solution, dried over sodium sulphate and concentrated.
The oily residue is then purified by eluting on 140 g of silica gel with a mixture of methylene chloride/ethanol 9:1.
The pure 1'-desoxy~ (2-chloro-6-cyclopentylamino-9-pu-rinyl)-2',3'-isopropylidene-3-D-ribose has a Rf value of 0.5.
b) 8.7 g of 1'-desoxy-1'-(2-chloro-6-cyclopentylamino-9-pu-rinyl)2',3'-isopropylidene-~-D-ribose and 30.5 g of pyri-dinium dichromate are stirred for 18 hours at room tem-perature in 130 ml of dimethylformamide. The mixture is then poured onto water and the aqueous phase is shaken out three times with ethyl acetate. This phase is then extracted with a saturated, aqueous solution of sodium hydrogen carbonate, the basic extract i9 ad~usted to pH 1 with 5N hydrochloric acid and shaken out with ethyl acetate. After washing with saturated sodium chloride solution and drying over sodium sulphate, the organic phase is concentrated, diluted with diethylether, whereby the l'-desoxy-1'-(2-chloro-6-cyclo-pentylamino-9-purinyl)-~-D-ribofuranuronic acid crystallises out. M.p. 246-253.
c) 4 g of the above acid are heated in 40 ml of thionyl chloride for 20 minutes in an oil bath of 45. Uhen the evolution of gas has ended, the mixture is concentrated under reduced pressure and the acid chloride formed is dissolved in 40 ml of methylene chloride. It is then cooled - 20 - Case 100-7069 in an ice bath and gaseous ethylamine is introduced whilst stirring until the reaction becomes basic. The methylene chloride phase is then washed with water, dried over sodium sulphate and concentrated. The 1'-desoxy-1'-(2-chloro-6-cyclopentylamino-9-purinyl)-2',3'-isopropylidene-~-D-ribofu-ranuronic acid-N-ethylamide remains behind as a white foam.
Rf in methylene chloride/ethanol 9:1 = 0.7.
The preparation of compounds of formula II, R6 having the significance of R6"' and X being . O is performed starting from the above compound of formula IIa wherein R6' is chlorine in a manner known per se e.g. by reaction with an alcohol, amine etc.
Example 20: 1'-desoxy-1'-(2'-methyl-6-cyclopentylamino-9-purinyl)-e-D-ribofuranuronic acid-N-ethylthioamide a) 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2~,3'-isopropyliden-~-D-ribofuranuronic acid-N-ethylthioamide.
1.7 g of 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-puri-nyl)-2',3' isopropylidene-~-D-ribofuranuronic acid-N-ethyl-amide (starting compound of example 1) are stirred with 0.77 g of LA~ESSON'S REAGENT in 38 ml of toluene for 2 hours in an oil bath of 100. The mixture is subsequently totally concentrated under reduced pressure, the residue is dis-solved in 60 ml of ethyl acetate and stirred for 1/2 hour with 25 g of neutral aluminium oxide. After filtration, the filtrate is concentrated and is used in the next stage with-out further purification. Rf in ethyl acetate: 0.7.
b) l'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-~-D-ri-bofuranuronic acid-N-ethylthioamide.
- 21 - Case 100-7069 1.5 g of 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylthioamide are dissolved at room temperature in 7.5 ml of 90X trifluoroacetic acid and left to stand for 2 hours.
The solution is subsequently totally concentrated under reduced pressure. The residue is dissolved in ethyl acetate, mixed with aqueous ammonia and totally concentrated under reduced pressure. The crystalline residue of the compound named in the title, is then purified by eluting on 30 g silica gel with a mixture of methylene chloride~ethanol 9:1.
Finally, the pure fractions are crystallised from ethylether/pentane. M.p. 168-170.
The following compounds of formula I in which R1, R2, R3 and R6 are defined as follows and wherein X is always ~ S are obtained analogously to example 20.
Example R1 R2 R3 R6 M.P.
21 3-Pentyl H Et Me amorphous 22 p-Ethoxyphenyl H Et Me 202-205 23 p-Methoxyphenyl H Et Me 222-225 24 3,4-Dimethoxyphenyl H Et Me 221-224 4-Hethylsulfonylphenyl H Et Me 164-167 The compounds according to the invention are notable for their interesting pharmacological properties. They can therefore be used as medicaments.
In particular, the compounds according of the invention have anti-hypertensive activity, as can be deduced from the results of the following trials:
. ~
- 22 - Case 100-7069 Measurement of the binding to adenosine Al and A2 receptors in membranes from the rat's cortex or from the cerebral cortex or striatum of the pig, using the method of R.F. BRUNS, G.H. LU and T.A. PUGSLEY, which is desribed in MOLEC. PHARMACOL. 29, 331-346 (1986).
, . ~
- 23 - Case 100-7069 Further testing of the activity of the compounds according to the invention on the isolated, perfused rat's kindneys for the following parameters:
- renin secretion - renal haemodynamics (vasodilation) - inhibition of the release of noradrenaline from the nerve ends following electro-stimulation of the renal nerves according to ~he method of H.J. SCHUREK, J.P. BRECHT, H. LOHFERT and R. HIERHOLZER, described in COMMUNICATION a la REUNION de l'ASSOCIATION DES PHARMACOLOGISTES LO WAIN UCL
4th June 1977, as well P.M. VANHOUTTE, D. BRO~NING, E. COEN, T.J. VERBEUREN, L. ZONNEKEYEN and M.G. COLLINS described in HPYERTENSION 4, 251-256 (1982).
- measurement of blood pressure, heart rate, urine production and renin activity in the plasma of wake, NaCl-depleted, and -replated normotensive or spontaneously hypertensive rats which have catheters implanted in the abdominal aorta and the Vena cava, following i.v. administration or admi-nistration of the compounds according to the invention as an inusion or a bolus, according to the method of J.F.M. SMITS
and J.H. BRODY described in Am. J. Phyiol. 247, R1 003-R1 008 (1984).
From the results of the trials, it can be deduced that both an inhibition of renin secretion and of the release of nor-adrenaline from the nerve ends, and direct vasodilation, take part in the anti-hypertensive activity of the compounds according to ~he invention.
24 1 3 2 6 0 1 7 Case 100-7069 From this, it is evident that the compounds according to the invention can not only be used as anti-hypertensive agents, but can also effect coronary vasodilation. They protect further the vascular endothelium by inhibiting platelet aggregation and by activating leucocytes. They reduce also the blood lipid level.
For the above indications of the compounds according to the invention, the compound of example 2 is preferred.
For the above-mentioned application as anti-hypertensive agents, the dosage to be used varies according to the substance used, the type of administration and the desired treatment. In general however, satisfactory results are obtained with a daily dosage of approximately 0.01 to 10 mg per kg body weight; If necessary, administration may take place in 2 to 4 portions or even in sustained release form. For larger mammals, the daily dosage is in the range of approximately 10 to 500 mg; suitable dosage forms for e.g. oral or non-oral administration generally contain about 5 to 250 mg, together with solid or liquid carrier substances.
The compounds according to the invention may be administered alone or in suitable dosage form. The medicinal forms, e.g. a solution or a tablet, can be produced analogously to known methods.
The invention therefore relates also to medicaments which contain the compounds according to the invention in free form or in the form of their physiologically acceptable salts, as well as the produc~ion of these medicament~ in known manner. They can be produced by using conventional pharmaceutical adjuvants and carriers.
The compounds of formula II used as starting compounds are obtained by introducing an isopropyliden protecting group into compounds of formula III, HN~Rl R6 ~ ~ N III
~ \l H
IH IH
wherein Rl is defined as above and Rc' denotes halogen, (C1_4)alkyl or (C3_~)cycloalkyl, (described for example in DE-OS
1 670 175; BRIT. PAT 1 075 008 and JOC (1968) 2583), by reacting it with acetone in the presence of an acid, for example p-toluenesulphonic acid, whereby componds of formula IV
HN,Rl R ~ ~
~ 0 ~
HO ~ ~
o~o wherein R~ and R6' are defined as above, are obtained, then oxidising them in known manner to form compounds of formula V, - 10 - Case 100-7069 N~R~
Ro~ ) V
HOOC~
1~1 wherein Rl and R6' are defined as above, using an oxidation agent, for example pyridinium dichromate, and subsequently converting this in known manner, using a chlorination agent such as thionyl chloride, into the acid chloride of formula VI, R '~N~N~ ~
ClOC~ 'Jl 0><0 wherein Rl and R6' are defined as above, and then reacting this with a compound of formula VII, R2 ~ VII
wherein R2 and R3 have the definitions given above, in known manner, to form compounds of for0ula IIa Case 100-7069 HN ~R1 1`~N~
R 6 o ~ IIa R
(partial structure of compounds of formula II), wherein R1, R2, R3 and R6' are defined as above.
The radical R6"', which is defined as below, is introduced into compounds of formula IIa, wherein R6' is chlorine or bromine, by reacting them with compounds of formula HR6"' wherein R6"' denotes a cyano group or groups of formulae -OR4, ~ R4 SR4 or -N ' , wherein R4 and R~ are defined as above, in ~ R~
a strongly alkaline medium, for example in the presence of sodium, or by reacting them with the corresponding amines in an autoclave at temperatures of above 100C. The compounds of formula IIb H~Rl ~ ~ ~ IIb 2\ N ~ C
` 1326017 - 12 - Case 100-7069 (compounds of formula II, wherein X denotes = O and R6 comprises the significancies of both R6' and R6"') wherein Rl, R2, R3 and R6 are defined as above which are obtained according to the preceding steps are converted by appropriate thianation into compounds of formula IIc Rl L:;~
~6 5 ~ IIc ~6 R3 ~
(compounds of formula II, wherein X denote~ ~ S) wherein Rl, R2, R3 and R6 are defined as above.
The thianation process is suitably effected using known thianation agents, for example hydrogen sulphide, phosphorus pentasulphide or LAUESSON'S REAGENT (p-methoxyphenylthiophosphlne sulphide dimer). The latter reagent i9 preferred. The reaction itself takes place in known manner. If for example hydrogen sulphide is used, an acid ~uch as hydrochloric acid is con-veniently added in catalytic doses, and the reaction is carried out in a polar solvent such as acetic acid or ethanol. Uhen using LAUESSON'S REAGENT, the reaction is conveniently carried out in a dry solvent such as toluene or methylene chloride.
A further method for the production of compounds of formula IIb, wherein R6 is (Cl_4)alkyl, is that 1'-desoxy-1'(2-alkyl-6-hydroxy-9-purinyl)-~-D-ribose of formula IIIa, -` ~ 1326017 - 13 - Case 100-7069 OH
N~ ~) O ¦ IIla HO ~ ) OH OH
wherein R6~V is (C1_4)alkyl, is reacted with acetone in the pre-sence of an acid, for example p-toluenesulphonic acid, to form compounds of formula IVa, OH
R iv ~
6 C~ IVa ~<o wherein R61V is defined as above, then these are oxidised using an oxidatlon agent, for example potassium permanganate, ln an alkaline medium, to produce compounds of formula Va, OH
6 o ~
' ~I Va X
1 3260~ 7 - 14 - Case 100-7069 wherein R61V is defined as above, then these are treated with a chlorination agent, for example phosphorus oxichloride, thus being converted into compounds of formula VIa, Cl R6 i V ol~ ~
C ~ Vla O O
X
wherein R61V is defined as above, then these are reacted with the above-mentioned compounds of formula VII to produce compounds of formula VIIIa, R6~ 0~ ld X
wherein R2, R3 and RCiv are defined as above, and these are converted by reacting with compounds of formula X, Rl-NH2 X
wherein R1 is defined as given above, to form compounds of formula IIb, wherein R6 is (C1_4)alkyl. The compounds of formula IIb thus obtained, wherein R6 is (Cl_4)alkyl, may be converted by - 15 - Case 100-7069 thianation as described above into the corresponding compounds of formula II.
The other above-described reactions take place using knovn methods, for example also uslng the processes described in the examples.
If in the compounds of formula I, Rl denotes groups which re substituted by a -N - group, these compounds can - R~
form salts with strong acids. Preferred salts are the hydrochlorides, hydrobromides or fumarates.
Insofar as the production of the required starting materials is not described, these are known or may be produced by known processes, or analogously to the processes described here, or analogously to known processes.
In the following examples, all temperatures are given in degrees celsius and are uncorrected.
- 16 - Case 100-7069 Example 1 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-~-D-ribofuranuronic acid N-ethylamide 1.4 g of 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2',3'-isopropylidene-~_D-ribofuranuronic acid-N-ethyl-amide are left to stand for 2 hours at 0 and for 1 hour at room temperature in 10 ml of 90% trifluoroacetic acid. The mixture is then totally concentrated under reduced pressure and the residue is par~itioned between ethyl acetate and diluted, aqueous ammonia. After washing with saturated, aqueous sodium chloride solution, the product is dried over sodium sulphate and totally concentrated. The residue is dissolved in a little methanol and the final product is crystallised by adding ethylether. M.p.
195-197.
The 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2~,3~-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide used as the starting material may be produced e.g. as follows:
a) 7.5 g of potassium permanganate are added to a solution of 7.8 g of 1'-desoxy-1'-(2-methyl-6-hydroxy-9-purinyl)-2',3-isopropylidene-~-D-ribose in 120 ml of water and 4.8 ml of lON sodium hydroxide, and the mixture is stirred for 1 hour at 30. Then, 1 g of sodium hydrogen sulphite is added and the colourless solution is filtered over Hyflo after stirring for S minutes. The filtrate is then con-centrated to ca. 30 ml under reduced pressure, and set at pH 4 with concentrated hydrochloric acid at 0. 1'-deso-xy-1'-(2-methyl-6-hydroxy-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid is precipitated in crystalline form. M.p. after washing with acetone and drying: 263 (decomp.).
- 17 - Case 100-7069 b) 3.3 g f 1'-desoxy-1'-(2-methyl-6-hydroxy-9-purinyl)-2',3'-isopropylidene-k-D-ribofuranuronic acid are stirred into 16.8 ml of phosphorus oxychloride for 15 minutes in an oil bath of 85, then mixed with 1.6 ml of N,N-diethyl-aniline and stirred for a further 2 hours at the same temperature. The mixture is subsequently totally con-centrated under reduced pressure and the residue is dissolved in 60 ml of tetrahydrofuran. This solution is cooled to -40 and is mixed with ethylamine until a basic reaction takes place. After 10 minutes, the solution is poured onto ice water and shaken with ethyl acetate. After washing with saturated sodium chloride solution and drying over sodium sulphate, the solution is totally concentrated and the residue is eluted on silica gel with ethyl acetate. The purified l'-desoxy-1'-(2-methyl-6-chloro-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide is a white foam and in ethyl acetate has a Rf value of 0.5.
c) 1.2 g of 1'-tesoxy-1'-(2-methyl-6-chloro-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide and 1.2 ml of cyclopentylamine are stirred into 30 ml of dioxane for 1 hour in an oil bath of 105. After cooling, filtration i9 effected, the filtrate is concentrated and the residue is eluted on 60 g of silica gel with ethyl acetate. The pure l'-desoxy-1'-(2-methyl-6-cyclopentyl-amino-9-purinyl)-2',3'-isopropylidene-0-D-ribofuranuronic acid-N-ethylamide i9 obtained as a white foam. Rf value in ethyl acetate: 0.45.
The following compounds of formula I, in which Rl, R2, R~
and Rc are defined as follows and wherein X is always . 0 are obtained analogously to example 1:
~ 1 32601 7 - 18 - Case 100-7069 Example Rl R2 R3 R6 M.P.
2 p-Methoxyphenyl H Et Me 221-224 3 Cyclopentyl H Et Me 196-198 4 Cyclopentyl H Et Isopropyl amorphous Cyclopentyl H Et Cl 133-135 6 Cyclopentyl H Et Br 188-190 7 Cyclopentyl H Et MeO 226-229 8 Cyclopentyl H Et MeS amorphous 9 Cyclopentyl H Et Me2N amorphous Cyclopentyl H Et MeHN 193-194 11 H H Et Br 240-241 12 p-Ethoxyphenyl H Et Me 120-125 13 3,4-Dimethoxyphenyl H Et Me 236-239 14 3-Pentyl H Et Me 181-183 m-Fluorophenyl H Et Me 137-142 16 p-Fluorophenyl H Et Me 257-259 17 p-Chlorophenyl H Et Me 255-258 18 Isopropyl H Et Me 187-194 19 p-Trifluoromethylphenyl H Et Me 248-250 The l'-desoxy-1'-(2-chloro-6-cyclopentylamino-9-puri-nyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylamide also useful as the starting material for the preparation of compounds o formula II wherein R6 has the significance of R6"' may be produced e.g. as follows:
a) 8.8 ml of ortho-formic acid trimethylester is added in drops at room temperature to 7.4 g of 1'-desoxy-1'-t2-chloro-6-cyclopentylamino-9-purinyl)-~-D-ribose and 4.2 g of p-tolu-ene-sulphonic acid in 120 ml of acetone. After 3 hours, the - 19 - Case 100-7069 deposit is filtered off and washed with acetone and diethyl-ether. Then, the dried deposit is added in portions whilst stirring to a solution of 3.6 g of sodium hydrogen carbonate in 150 ml of water and 75 ml of ethyl acetate. The organic phase is separated, washed with saturated sodium chloride solution, dried over sodium sulphate and concentrated.
The oily residue is then purified by eluting on 140 g of silica gel with a mixture of methylene chloride/ethanol 9:1.
The pure 1'-desoxy~ (2-chloro-6-cyclopentylamino-9-pu-rinyl)-2',3'-isopropylidene-3-D-ribose has a Rf value of 0.5.
b) 8.7 g of 1'-desoxy-1'-(2-chloro-6-cyclopentylamino-9-pu-rinyl)2',3'-isopropylidene-~-D-ribose and 30.5 g of pyri-dinium dichromate are stirred for 18 hours at room tem-perature in 130 ml of dimethylformamide. The mixture is then poured onto water and the aqueous phase is shaken out three times with ethyl acetate. This phase is then extracted with a saturated, aqueous solution of sodium hydrogen carbonate, the basic extract i9 ad~usted to pH 1 with 5N hydrochloric acid and shaken out with ethyl acetate. After washing with saturated sodium chloride solution and drying over sodium sulphate, the organic phase is concentrated, diluted with diethylether, whereby the l'-desoxy-1'-(2-chloro-6-cyclo-pentylamino-9-purinyl)-~-D-ribofuranuronic acid crystallises out. M.p. 246-253.
c) 4 g of the above acid are heated in 40 ml of thionyl chloride for 20 minutes in an oil bath of 45. Uhen the evolution of gas has ended, the mixture is concentrated under reduced pressure and the acid chloride formed is dissolved in 40 ml of methylene chloride. It is then cooled - 20 - Case 100-7069 in an ice bath and gaseous ethylamine is introduced whilst stirring until the reaction becomes basic. The methylene chloride phase is then washed with water, dried over sodium sulphate and concentrated. The 1'-desoxy-1'-(2-chloro-6-cyclopentylamino-9-purinyl)-2',3'-isopropylidene-~-D-ribofu-ranuronic acid-N-ethylamide remains behind as a white foam.
Rf in methylene chloride/ethanol 9:1 = 0.7.
The preparation of compounds of formula II, R6 having the significance of R6"' and X being . O is performed starting from the above compound of formula IIa wherein R6' is chlorine in a manner known per se e.g. by reaction with an alcohol, amine etc.
Example 20: 1'-desoxy-1'-(2'-methyl-6-cyclopentylamino-9-purinyl)-e-D-ribofuranuronic acid-N-ethylthioamide a) 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2~,3'-isopropyliden-~-D-ribofuranuronic acid-N-ethylthioamide.
1.7 g of 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-puri-nyl)-2',3' isopropylidene-~-D-ribofuranuronic acid-N-ethyl-amide (starting compound of example 1) are stirred with 0.77 g of LA~ESSON'S REAGENT in 38 ml of toluene for 2 hours in an oil bath of 100. The mixture is subsequently totally concentrated under reduced pressure, the residue is dis-solved in 60 ml of ethyl acetate and stirred for 1/2 hour with 25 g of neutral aluminium oxide. After filtration, the filtrate is concentrated and is used in the next stage with-out further purification. Rf in ethyl acetate: 0.7.
b) l'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-~-D-ri-bofuranuronic acid-N-ethylthioamide.
- 21 - Case 100-7069 1.5 g of 1'-desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-2',3'-isopropylidene-~-D-ribofuranuronic acid-N-ethylthioamide are dissolved at room temperature in 7.5 ml of 90X trifluoroacetic acid and left to stand for 2 hours.
The solution is subsequently totally concentrated under reduced pressure. The residue is dissolved in ethyl acetate, mixed with aqueous ammonia and totally concentrated under reduced pressure. The crystalline residue of the compound named in the title, is then purified by eluting on 30 g silica gel with a mixture of methylene chloride~ethanol 9:1.
Finally, the pure fractions are crystallised from ethylether/pentane. M.p. 168-170.
The following compounds of formula I in which R1, R2, R3 and R6 are defined as follows and wherein X is always ~ S are obtained analogously to example 20.
Example R1 R2 R3 R6 M.P.
21 3-Pentyl H Et Me amorphous 22 p-Ethoxyphenyl H Et Me 202-205 23 p-Methoxyphenyl H Et Me 222-225 24 3,4-Dimethoxyphenyl H Et Me 221-224 4-Hethylsulfonylphenyl H Et Me 164-167 The compounds according to the invention are notable for their interesting pharmacological properties. They can therefore be used as medicaments.
In particular, the compounds according of the invention have anti-hypertensive activity, as can be deduced from the results of the following trials:
. ~
- 22 - Case 100-7069 Measurement of the binding to adenosine Al and A2 receptors in membranes from the rat's cortex or from the cerebral cortex or striatum of the pig, using the method of R.F. BRUNS, G.H. LU and T.A. PUGSLEY, which is desribed in MOLEC. PHARMACOL. 29, 331-346 (1986).
, . ~
- 23 - Case 100-7069 Further testing of the activity of the compounds according to the invention on the isolated, perfused rat's kindneys for the following parameters:
- renin secretion - renal haemodynamics (vasodilation) - inhibition of the release of noradrenaline from the nerve ends following electro-stimulation of the renal nerves according to ~he method of H.J. SCHUREK, J.P. BRECHT, H. LOHFERT and R. HIERHOLZER, described in COMMUNICATION a la REUNION de l'ASSOCIATION DES PHARMACOLOGISTES LO WAIN UCL
4th June 1977, as well P.M. VANHOUTTE, D. BRO~NING, E. COEN, T.J. VERBEUREN, L. ZONNEKEYEN and M.G. COLLINS described in HPYERTENSION 4, 251-256 (1982).
- measurement of blood pressure, heart rate, urine production and renin activity in the plasma of wake, NaCl-depleted, and -replated normotensive or spontaneously hypertensive rats which have catheters implanted in the abdominal aorta and the Vena cava, following i.v. administration or admi-nistration of the compounds according to the invention as an inusion or a bolus, according to the method of J.F.M. SMITS
and J.H. BRODY described in Am. J. Phyiol. 247, R1 003-R1 008 (1984).
From the results of the trials, it can be deduced that both an inhibition of renin secretion and of the release of nor-adrenaline from the nerve ends, and direct vasodilation, take part in the anti-hypertensive activity of the compounds according to ~he invention.
24 1 3 2 6 0 1 7 Case 100-7069 From this, it is evident that the compounds according to the invention can not only be used as anti-hypertensive agents, but can also effect coronary vasodilation. They protect further the vascular endothelium by inhibiting platelet aggregation and by activating leucocytes. They reduce also the blood lipid level.
For the above indications of the compounds according to the invention, the compound of example 2 is preferred.
For the above-mentioned application as anti-hypertensive agents, the dosage to be used varies according to the substance used, the type of administration and the desired treatment. In general however, satisfactory results are obtained with a daily dosage of approximately 0.01 to 10 mg per kg body weight; If necessary, administration may take place in 2 to 4 portions or even in sustained release form. For larger mammals, the daily dosage is in the range of approximately 10 to 500 mg; suitable dosage forms for e.g. oral or non-oral administration generally contain about 5 to 250 mg, together with solid or liquid carrier substances.
The compounds according to the invention may be administered alone or in suitable dosage form. The medicinal forms, e.g. a solution or a tablet, can be produced analogously to known methods.
The invention therefore relates also to medicaments which contain the compounds according to the invention in free form or in the form of their physiologically acceptable salts, as well as the produc~ion of these medicament~ in known manner. They can be produced by using conventional pharmaceutical adjuvants and carriers.
Claims (4)
1. A compound of the formula:
wherein R1 signifies (C1-6) alkyl; (C3-7) cycloalkyl which is unsubstituted or mono-substituted by a hydroxyl group; or phenyl which is unsubstituted or mono- or di-substituted by halogen with an atomic number of 9-35, (C1-4)alkyl, (C1-4)alkoxy, hydroxy or trifluoromethyl, R2 signifies hydrogen, (C1-4)alkyl or (C3-8) cycloalkyl, R3 is hydrogen or (C1-4)alkyl, R6 is halogen, (C1-4)alkyl, -OR4, or -SR4, wherein R4 is (C1-4)alkyl and X signifies =S or O
with the provisio that when X signifies O, R6 cannot be halogen.
wherein R1 signifies (C1-6) alkyl; (C3-7) cycloalkyl which is unsubstituted or mono-substituted by a hydroxyl group; or phenyl which is unsubstituted or mono- or di-substituted by halogen with an atomic number of 9-35, (C1-4)alkyl, (C1-4)alkoxy, hydroxy or trifluoromethyl, R2 signifies hydrogen, (C1-4)alkyl or (C3-8) cycloalkyl, R3 is hydrogen or (C1-4)alkyl, R6 is halogen, (C1-4)alkyl, -OR4, or -SR4, wherein R4 is (C1-4)alkyl and X signifies =S or O
with the provisio that when X signifies O, R6 cannot be halogen.
2. A compound selected from:
a) 1'-Desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide;
b) 1'-Desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
c) 1'-Desoxy-1'-(2-ethyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
d) 1'-Desoxy-1'-(2-isopropyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
e) 1'-Desoxy-1'-(2-methyl-6-p-methoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
f) 1'-Desoxy-1'-(2-methylthio-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
g) 1'-Desoxy-1'-(2-methoxy-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
h) 1'-Desoxy-1'-(2-methyl-6-p-ethoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
i) 1'-Desoxy-1'-(2-methyl-6-(3,4-dimethoxyphenyl)amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
j) 1'-Desoxy-1'-(2-methyl-6-(3-pentyl)-amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
k) 1'-Desoxy-1'-(2-methyl-6-m-fluorophenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
l) 1'-Desoxy-1'-(2-methyl-6-p-fluorophenylamino-9-pur.beta.inyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
m) 1'-Desoxy-1'-(2-methyl-6-p-chlorophenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
n) 1'-Desoxy-1'-(2-methyl-6-isopropylamino-9-purinyl)-B-D-ribofuranuronic acid-N-ethylamide;
o) 1'-Desoxy-1'-(2-methyl-6-p-trifluoromethylphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
p) 1'-Desoxy-1'-(2-methyl-6-(3-pentyl)-amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide;
q) l'-Desoxy-1'-(2-~ethyl-6-p-ethoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide;
r) 1'-Desoxy-1'-(2-methyl-6-p-methoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide; and s) 1'-Desoxy-1'-(2-methyl-6-(3,4-dimethoxyphenyl)-amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide.
a) 1'-Desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide;
b) 1'-Desoxy-1'-(2-methyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
c) 1'-Desoxy-1'-(2-ethyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
d) 1'-Desoxy-1'-(2-isopropyl-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
e) 1'-Desoxy-1'-(2-methyl-6-p-methoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
f) 1'-Desoxy-1'-(2-methylthio-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
g) 1'-Desoxy-1'-(2-methoxy-6-cyclopentylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
h) 1'-Desoxy-1'-(2-methyl-6-p-ethoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
i) 1'-Desoxy-1'-(2-methyl-6-(3,4-dimethoxyphenyl)amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
j) 1'-Desoxy-1'-(2-methyl-6-(3-pentyl)-amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
k) 1'-Desoxy-1'-(2-methyl-6-m-fluorophenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
l) 1'-Desoxy-1'-(2-methyl-6-p-fluorophenylamino-9-pur.beta.inyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
m) 1'-Desoxy-1'-(2-methyl-6-p-chlorophenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
n) 1'-Desoxy-1'-(2-methyl-6-isopropylamino-9-purinyl)-B-D-ribofuranuronic acid-N-ethylamide;
o) 1'-Desoxy-1'-(2-methyl-6-p-trifluoromethylphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylamide;
p) 1'-Desoxy-1'-(2-methyl-6-(3-pentyl)-amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide;
q) l'-Desoxy-1'-(2-~ethyl-6-p-ethoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide;
r) 1'-Desoxy-1'-(2-methyl-6-p-methoxyphenylamino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide; and s) 1'-Desoxy-1'-(2-methyl-6-(3,4-dimethoxyphenyl)-amino-9-purinyl)-.beta.-D-ribofuranuronic acid-N-ethylthioamide.
3. Use of a compound as claimed in claim 1 or 2 for the treatment of raised blood pressures.
4. Pharmaoeutical composition containing as an active agent a compound of claim 1 or 2 in association with a pharmacologically acceptable adjuvant and/or diluent useful for the treatment of raised blood pressures.
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP3711564.2 | 1987-04-06 | ||
DE3711564 | 1987-04-06 | ||
DEP3711562.6 | 1987-04-06 | ||
DE3711562 | 1987-04-06 | ||
DE3711563 | 1987-04-06 | ||
DEP3711563.4 | 1987-04-06 | ||
DEP3711561.8 | 1987-04-06 | ||
DE3711561 | 1987-04-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1326017C true CA1326017C (en) | 1994-01-11 |
Family
ID=27433888
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000563261A Expired - Fee Related CA1326017C (en) | 1987-04-06 | 1988-04-05 | Ribofuranuronic acid derivatives |
Country Status (22)
Country | Link |
---|---|
JP (1) | JPS63258892A (en) |
KR (1) | KR880012629A (en) |
AT (1) | AT393507B (en) |
AU (1) | AU609109B2 (en) |
BE (1) | BE1002151A5 (en) |
CA (1) | CA1326017C (en) |
CH (1) | CH676121A5 (en) |
DK (1) | DK183488A (en) |
ES (1) | ES2007177A6 (en) |
FI (1) | FI87463C (en) |
FR (1) | FR2613367A1 (en) |
GB (1) | GB2203149B (en) |
HU (1) | HU201955B (en) |
IL (1) | IL85969A (en) |
IT (1) | IT1219892B (en) |
LU (1) | LU87181A1 (en) |
MY (1) | MY102323A (en) |
NL (1) | NL8800862A (en) |
NZ (1) | NZ224131A (en) |
PH (1) | PH25424A (en) |
PT (1) | PT87153B (en) |
SE (1) | SE8801236L (en) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4755594A (en) * | 1986-01-31 | 1988-07-05 | Warner-Lambert Company | N6 -substituted adenosines |
US4954504A (en) * | 1986-11-14 | 1990-09-04 | Ciba-Geigy Corporation | N9 -cyclopentyl-substituted adenine derivatives having adenosine-2 receptor stimulating activity |
US5063233A (en) * | 1986-11-14 | 1991-11-05 | Ciba-Geigy Corporation | N9 -cyclopentyl-substituted adenine derivatives useful as adenosine receptor agonists |
NL8702926A (en) * | 1986-12-15 | 1988-07-01 | Sandoz Ag | NEW FURANURONIC ACID DERIVATIVES AND METHODS FOR PREPARING AND USING THESE DERIVATIVES. |
US4968697A (en) * | 1987-02-04 | 1990-11-06 | Ciba-Geigy Corporation | 2-substituted adenosine 5'-carboxamides as antihypertensive agents |
PT86660B (en) * | 1987-02-04 | 1992-02-28 | Ciba Geigy Ag | PROCESS FOR THE PREPARATION OF ADENOSIN-5'-CARBOXAMIDE DERIVATIVES |
USRE36494E (en) * | 1990-02-20 | 2000-01-11 | Discovery Therapeutics, Inc. | 2-aralkoxy and 2-alkoxy adenosine derivatives as coronary vasodilators and antihypertensive agents |
US5140015A (en) * | 1990-02-20 | 1992-08-18 | Whitby Research, Inc. | 2-aralkoxy and 2-alkoxy adenosine derivatives as coronary vasodilators and antihypertensive agents |
IT1254915B (en) * | 1992-04-24 | 1995-10-11 | Gloria Cristalli | ADENOSINE DERIVATIVES FOR ACTIVITY A2 AGONIST |
GB9301000D0 (en) * | 1993-01-20 | 1993-03-10 | Glaxo Group Ltd | Chemical compounds |
TW528755B (en) | 1996-12-24 | 2003-04-21 | Glaxo Group Ltd | 2-(purin-9-yl)-tetrahydrofuran-3,4-diol derivatives |
YU44900A (en) | 1998-01-31 | 2003-01-31 | Glaxo Group Limited | 2-(purin-9-yl)-tetrahydrofuran-3,4-diol derivatives |
AU750462B2 (en) | 1998-06-23 | 2002-07-18 | Glaxo Group Limited | 2-(purin-9-yl)-tetrahydrofuran-3,4-diol derivatives |
AU4343500A (en) | 1999-04-16 | 2000-11-02 | Schering Corporation | Use of azetidinone compounds |
US20050033044A1 (en) | 2003-05-19 | 2005-02-10 | Bristol-Myers Squibb Pharma Company | Methods for preparing 2-alkynyladenosine derivatives |
AR049384A1 (en) | 2004-05-24 | 2006-07-26 | Glaxo Group Ltd | PURINA DERIVATIVES |
AU2005286946B2 (en) * | 2004-09-20 | 2012-03-15 | Inotek Pharmaceuticals Corporation | Purine derivatives and methods of use thereof |
GB0514809D0 (en) | 2005-07-19 | 2005-08-24 | Glaxo Group Ltd | Compounds |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH606084A5 (en) * | 1975-02-18 | 1978-10-13 | Hoffmann La Roche | (Di) nitrates of adenosine -5'-carboxylic acid derivs |
US4167565A (en) * | 1976-11-08 | 1979-09-11 | Abbott Laboratories | Adenosine-5'-carboxamides and method of use |
DE3406533A1 (en) * | 1984-02-23 | 1985-08-29 | Boehringer Mannheim Gmbh, 6800 Mannheim | USE OF ADENOSINE DERIVATIVES AS ANTIALLERGICA AND MEDICINAL PRODUCTS CONTAINING THEM |
NL8702926A (en) * | 1986-12-15 | 1988-07-01 | Sandoz Ag | NEW FURANURONIC ACID DERIVATIVES AND METHODS FOR PREPARING AND USING THESE DERIVATIVES. |
PT86660B (en) * | 1987-02-04 | 1992-02-28 | Ciba Geigy Ag | PROCESS FOR THE PREPARATION OF ADENOSIN-5'-CARBOXAMIDE DERIVATIVES |
-
1988
- 1988-03-28 LU LU87181A patent/LU87181A1/en unknown
- 1988-03-30 BE BE8800374A patent/BE1002151A5/en not_active IP Right Cessation
- 1988-03-30 FR FR8804356A patent/FR2613367A1/en not_active Withdrawn
- 1988-03-30 IT IT47794/88A patent/IT1219892B/en active
- 1988-03-31 CH CH1228/88A patent/CH676121A5/de not_active IP Right Cessation
- 1988-03-31 GB GB8807750A patent/GB2203149B/en not_active Expired - Lifetime
- 1988-04-04 PT PT87153A patent/PT87153B/en active IP Right Grant
- 1988-04-04 PH PH36731A patent/PH25424A/en unknown
- 1988-04-04 MY MYPI88000344A patent/MY102323A/en unknown
- 1988-04-04 KR KR1019880003819A patent/KR880012629A/en not_active Application Discontinuation
- 1988-04-04 IL IL85969A patent/IL85969A/en not_active IP Right Cessation
- 1988-04-05 SE SE8801236A patent/SE8801236L/en unknown
- 1988-04-05 HU HU881638A patent/HU201955B/en not_active IP Right Cessation
- 1988-04-05 JP JP63084974A patent/JPS63258892A/en active Pending
- 1988-04-05 FI FI881571A patent/FI87463C/en not_active IP Right Cessation
- 1988-04-05 NL NL8800862A patent/NL8800862A/en not_active Application Discontinuation
- 1988-04-05 CA CA000563261A patent/CA1326017C/en not_active Expired - Fee Related
- 1988-04-05 DK DK183488A patent/DK183488A/en not_active Application Discontinuation
- 1988-04-05 AT AT873/88A patent/AT393507B/en not_active IP Right Cessation
- 1988-04-05 AU AU14151/88A patent/AU609109B2/en not_active Ceased
- 1988-04-05 ES ES8801031A patent/ES2007177A6/en not_active Expired
- 1988-04-06 NZ NZ224131A patent/NZ224131A/en unknown
Also Published As
Publication number | Publication date |
---|---|
CH676121A5 (en) | 1990-12-14 |
KR880012629A (en) | 1988-11-28 |
GB8807750D0 (en) | 1988-05-05 |
SE8801236A0 (en) | 1988-10-17 |
FI881571A0 (en) | 1988-04-05 |
PT87153A (en) | 1988-05-01 |
JPS63258892A (en) | 1988-10-26 |
NL8800862A (en) | 1988-11-01 |
BE1002151A5 (en) | 1990-08-07 |
FI881571A (en) | 1988-10-07 |
PT87153B (en) | 1992-07-31 |
DK183488A (en) | 1988-10-07 |
FI87463C (en) | 1993-01-11 |
AU609109B2 (en) | 1991-04-26 |
GB2203149B (en) | 1991-02-13 |
MY102323A (en) | 1992-05-28 |
NZ224131A (en) | 1991-12-23 |
ATA87388A (en) | 1991-04-15 |
HUT48902A (en) | 1989-07-28 |
PH25424A (en) | 1991-07-01 |
IL85969A (en) | 1992-03-29 |
FR2613367A1 (en) | 1988-10-07 |
SE8801236L (en) | 1988-10-17 |
SE8801236D0 (en) | 1988-04-05 |
LU87181A1 (en) | 1988-11-17 |
IL85969A0 (en) | 1988-09-30 |
HU201955B (en) | 1991-01-28 |
AU1415188A (en) | 1988-10-06 |
FI87463B (en) | 1992-09-30 |
ES2007177A6 (en) | 1989-06-01 |
IT1219892B (en) | 1990-05-24 |
GB2203149A (en) | 1988-10-12 |
AT393507B (en) | 1991-11-11 |
IT8847794A0 (en) | 1988-03-30 |
DK183488D0 (en) | 1988-04-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA1326017C (en) | Ribofuranuronic acid derivatives | |
US5219840A (en) | Antihypertensive 9-(2,N6 -disubstituted adenyl) ribofuranuronic acid derivatives | |
US5464826A (en) | Method of treating tumors in mammals with 2',2'-difluoronucleosides | |
CA1287349C (en) | Rebeccamycin analogs | |
JPH052671B2 (en) | ||
AU602909B2 (en) | Pyrimidine derivatives | |
EP0291917B1 (en) | Novel oxetanocins | |
US5384310A (en) | 2'-fluoro-2-haloarabinoadinosines and their pharmaceutical compositions | |
US20080300399A1 (en) | Processes related to making capecitabine | |
AU590409B2 (en) | Aminoglycoside steroids | |
EP0143557B1 (en) | Griseolic acid derivatives, and their use | |
EP0196618A1 (en) | 4'-Demethyl-4-epipodophyllotoxin Derivatives, a process for their preparation and their use as medicaments | |
CA1291752C (en) | Furanuronic acid derivatives, process for their production and their use | |
WO1987004167A1 (en) | Aminoglycoside steroids, a process for their preparation, their use and pharmaceutical compositions containing them | |
US5534503A (en) | 2'-O, N6 -disubstituted and 2'-O, N6, 2-trisubstituted adenosines and their medicinal uses | |
IE60470B1 (en) | Pyrimidine derivatives, a process for their manufacture and medicaments containing said derivatives | |
WO2006122207A1 (en) | 6-hydrazinopurine 2'-methyl ribonucleosides and nucleotides for treatment of hcv | |
US5214048A (en) | Oxetanocins | |
HU203553B (en) | New process for producing oxetanocin g | |
EP0135271A1 (en) | Spectinomycin analogues and their preparation | |
JPH0633263B2 (en) | 7-Hydroxyguanine derivative, method for producing the same, and antitumor agent containing the same | |
CA2069714A1 (en) | Preparation of 6-0-acylelsamicin a derivatives | |
JPH06116267A (en) | 6'-c-alkyl or alkynylneplanocin a, its production and use |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MKLA | Lapsed |