CA1321157C - Plantes genetiquement ameliorees, a plus grande resistance aux maladies - Google Patents
Plantes genetiquement ameliorees, a plus grande resistance aux maladiesInfo
- Publication number
- CA1321157C CA1321157C CA000582005A CA582005A CA1321157C CA 1321157 C CA1321157 C CA 1321157C CA 000582005 A CA000582005 A CA 000582005A CA 582005 A CA582005 A CA 582005A CA 1321157 C CA1321157 C CA 1321157C
- Authority
- CA
- Canada
- Prior art keywords
- amino acid
- plant
- lytic peptide
- acid sequence
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8253—Methionine or cysteine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8254—Tryptophan or lysine
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Insects & Arthropods (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11594187A | 1987-11-02 | 1987-11-02 | |
US115,941 | 1987-11-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1321157C true CA1321157C (fr) | 1993-08-10 |
Family
ID=22364287
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000582005A Expired - Fee Related CA1321157C (fr) | 1987-11-02 | 1988-11-02 | Plantes genetiquement ameliorees, a plus grande resistance aux maladies |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0675960A1 (fr) |
KR (1) | KR890701005A (fr) |
AU (1) | AU2802989A (fr) |
CA (1) | CA1321157C (fr) |
WO (1) | WO1989004371A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6100453A (en) * | 1992-09-30 | 2000-08-08 | Cornell Research Foundation, Inc. | Transgenic pomaceous fruit with fire blight resistance |
Families Citing this family (50)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR970006154B1 (ko) * | 1987-07-06 | 1997-04-24 | 루이지아나 스테이트 유니버시티 아그리컬춰럴 앤드 메카니칼 컬리지 | 용해성 펩티드에 의한 진핵 병원체와 신생물의 억제 및 섬유아세포와 임파구의 자극 |
EP1004595B1 (fr) * | 1989-04-10 | 2003-06-18 | Helix BioMedix, Inc. | Peptides lytiques et prolifératifs et leur emploi comme agents pharmaceutiques et phytopharmaceutiques |
CA2030779A1 (fr) * | 1989-04-11 | 1990-10-12 | Huw M. Davies | Utilisation de peptides antimicrobiens d'origine animale pour lutter contre les agents pathogenes des plantes |
US5550318A (en) * | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
AU644097B2 (en) * | 1989-08-09 | 1993-12-02 | Monsanto Technology, Llc | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
DE3926390A1 (de) * | 1989-08-10 | 1991-02-14 | Bayer Ag | Verwendung von lysozym genen in pflanzen zur resistenzerhoehung |
GR1001135B (el) * | 1989-09-05 | 1993-04-28 | Dusar Heinz | Διαταξις δια την συγκρατηση μιας κουρτινας (παραπετασματος) ντους. |
US5177308A (en) * | 1989-11-29 | 1993-01-05 | Agracetus | Insecticidal toxins in plants |
US5484956A (en) * | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
JP3209744B2 (ja) * | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | 結実能力のある遺伝子変換コーン |
US5459237A (en) * | 1990-02-08 | 1995-10-17 | Magainin Pharmaceuticals Inc. | Peptide compositions and uses therefor |
US5631007A (en) * | 1990-03-12 | 1997-05-20 | Ciba-Geigy Corporation | Anti-pathogenically effective compositions comprising lytic peptides and hydrolytic enzymes |
PH30997A (en) * | 1990-03-12 | 1997-12-23 | Ciba Geigy | Antipathologenically effective compositions comprising lytic peptides and hydrolytic enzymes. |
CA2042448A1 (fr) * | 1990-06-05 | 1991-12-06 | Jonathan P. Duvick | Peptides antimicrobiens et resistance des plantes a la maladie ainsi obtenue |
US5421839A (en) * | 1990-06-15 | 1995-06-06 | Hoechst Aktiengesellschaft | Antifungal polypeptide and process for its production |
US5294605A (en) * | 1990-07-19 | 1994-03-15 | The Scripps Research Institute | Amphiphilic peptide compositions and analogues thereof |
FR2665177B1 (fr) * | 1990-07-24 | 1994-09-02 | Sanofi Sa | Gene recombinant codant pour une proteine a activite endochitinase et/ou lysozyme. |
JPH07242696A (ja) * | 1990-08-10 | 1995-09-19 | Enichem Partecipazioni Spa | 植物病原体に対して活性な抗菌性ペプチド、その使用法およびこれに関連するスクリーニング法 |
DE4040954C2 (de) * | 1990-12-20 | 2001-05-17 | Max Planck Gesellschaft | Verfahren zur Erzeugung von pathogen-resistenten Pflanzen |
US5235038A (en) * | 1991-01-22 | 1993-08-10 | Torry Pines Institute For Molecular Studies | Deletion and substitution analogues of melittin peptide |
AU648140B2 (en) * | 1991-02-01 | 1994-04-14 | Virtual Drug Development, Inc. | Reverse antimicrobial peptides and antimicrobial compositions |
GB9115669D0 (en) * | 1991-07-19 | 1991-09-04 | Sandoz Ltd | Improvements in or relating to organic compounds |
CA2114788C (fr) * | 1991-08-09 | 2004-12-21 | Saverio C. Falco | Proteines de stockage synthetiques a structure definie contenant des quantites programmables d'acides amines essentiels pour l'amelioration de la valeur nutritive des vegetaux |
CA2118939A1 (fr) * | 1991-09-13 | 1993-04-01 | W. Lee Maloy | Compositions de peptides amphiphiles biologiquement actives et leur utilisation |
US5422108A (en) * | 1991-09-19 | 1995-06-06 | Smart Plants International Inc. | Protection of plants against plant pathogens |
US5850025A (en) * | 1991-09-19 | 1998-12-15 | Sibia Neurosciences, Inc. | Protection of plants against plant pathogens |
GB9127250D0 (en) * | 1991-12-23 | 1992-02-19 | Unilever Plc | Modification |
USRE39238E1 (en) | 1992-10-09 | 2006-08-15 | Max-Planck-Gesellschaft zür Forderung der Wissenschaften e.V. | Transgenic pathogen-resistant organism |
DE4234131C2 (de) * | 1992-10-09 | 1995-08-24 | Max Planck Gesellschaft | Transgener pathogen-resistenter Organismus |
AU5463694A (en) * | 1992-11-05 | 1994-05-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Process for increasing the protein content of plants |
CA2161881C (fr) * | 1993-01-13 | 2001-03-27 | A. Gururaj Rao | Derives de l'alpha-hordothionine a forte teneur en lysine |
US5607914A (en) * | 1993-01-13 | 1997-03-04 | Pioneer Hi-Bred International, Inc. | Synthetic antimicrobial peptides |
US5464944A (en) * | 1993-06-21 | 1995-11-07 | Pioneer Hi-Bred International, Inc. | Synthetic antifungal peptides |
US5847047A (en) * | 1993-06-22 | 1998-12-08 | E. I. Du Pont De Nemours And Company | Antimicrobial composition of a polymer and a peptide forming amphiphilic helices of the magainin-type |
US5530187A (en) * | 1993-07-16 | 1996-06-25 | The Salk Institute For Biological Studies | Transgenic plants containing multiple disease resistance genes |
US5856178A (en) * | 1993-08-30 | 1999-01-05 | Utah State University | DNA cassettes for expression of lytic peptides in mammalian cells and transgenic organisms containing same |
US5580852A (en) * | 1993-12-17 | 1996-12-03 | Pioneer Hi-Bred International, Inc. | Derivatives of tachyplesin having inhibitory activity towards plant pathogenic fungi |
IL114697A0 (en) * | 1994-07-22 | 1995-11-27 | Demeter Biotech Ltd | Polypeptides comprising ubiquitin-lytic peptide fusion protein products deriving therefrom their preparation and use |
US5589364A (en) * | 1994-07-29 | 1996-12-31 | Magainin Pharmaceuticals Inc. | Recombinant production of biologically active peptides and proteins |
WO1996038574A1 (fr) * | 1995-05-31 | 1996-12-05 | Pioneer Hi-Bred International, Inc. | Procedes d'augmentation de l'accumulation d'acides amines essentiels dans des graines |
HUP9900878A2 (hu) * | 1995-06-02 | 1999-07-28 | Pioneer Hi-Bred International, Inc. | Alfa-hordotionin nagy metionintartalmú származéka |
BR9609200A (pt) * | 1995-06-02 | 1999-05-11 | Pioneer Hi Bred Int | Derivados de alta treonina de alpha-hordotionina |
US6077811A (en) * | 1995-06-06 | 2000-06-20 | Mitchell; James P. | Vanadium compositions to enhance plant growth and edible fruit yield |
US6750381B2 (en) * | 1996-03-25 | 2004-06-15 | National Institute Of Agrobiological Sciences | Pathogen-resistant plants transformed with a DNA encoding sarcotoxin 1A linked to a signal peptide and a method for production thereof |
JP2000516812A (ja) * | 1996-08-14 | 2000-12-19 | ノバルティス アクチエンゲゼルシャフト | 植物病原性真菌に対する阻害活性を有するペプチド |
DE19749973C1 (de) * | 1997-11-05 | 1998-10-22 | Klaus Dr Duering | Lysozym-analoge Proteine und Peptide mit antimikrobieller Wirkung, ihre Herstellung und ihre Verwendung |
US7053282B1 (en) * | 1998-02-09 | 2006-05-30 | Pioneer Hi-Bred International, Inc. | Alteration of amino acid compositions in seeds |
AU3868199A (en) * | 1998-04-27 | 1999-11-16 | Demegen, Inc. | A method for increasing the protein content of plants |
EP1174027A1 (fr) * | 2000-07-17 | 2002-01-23 | HOM Consultancy B.V. | Utilisations de peptides antimicrobiens |
WO2002063975A2 (fr) * | 2001-02-14 | 2002-08-22 | Ventria Bioscience | Compositions d'additif alimentaire et methodes associees |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR8600161A (pt) * | 1985-01-18 | 1986-09-23 | Plant Genetic Systems Nv | Gene quimerico,vetores de plasmidio hibrido,intermediario,processo para controlar insetos em agricultura ou horticultura,composicao inseticida,processo para transformar celulas de plantas para expressar uma toxina de polipeptideo produzida por bacillus thuringiensis,planta,semente de planta,cultura de celulas e plasmidio |
JPH02502513A (ja) * | 1986-07-25 | 1990-08-16 | ザ ボード オブ スーパーバイザーズ オブ ルイジアナ ステート ユニバーシティ アンド アグリカルチュラル アンド メカニカル カレッジ | 病気抵抗体及び害虫抵抗体を植物に組み込む方法並びに植物に組み込まれ該抵抗体の暗号に対応する新規な遺伝子 |
-
1988
- 1988-11-02 EP EP89900103A patent/EP0675960A1/fr not_active Withdrawn
- 1988-11-02 WO PCT/US1988/003908 patent/WO1989004371A1/fr not_active Application Discontinuation
- 1988-11-02 CA CA000582005A patent/CA1321157C/fr not_active Expired - Fee Related
- 1988-11-02 AU AU28029/89A patent/AU2802989A/en not_active Abandoned
-
1989
- 1989-07-03 KR KR1019890701236A patent/KR890701005A/ko not_active Application Discontinuation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6100453A (en) * | 1992-09-30 | 2000-08-08 | Cornell Research Foundation, Inc. | Transgenic pomaceous fruit with fire blight resistance |
Also Published As
Publication number | Publication date |
---|---|
AU2802989A (en) | 1989-06-01 |
EP0675960A4 (fr) | 1995-04-13 |
WO1989004371A1 (fr) | 1989-05-18 |
KR890701005A (ko) | 1989-12-19 |
EP0675960A1 (fr) | 1995-10-11 |
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