CA1251386C - Selection a l'aide de genes codant pour une opine syntethase - Google Patents
Selection a l'aide de genes codant pour une opine syntethaseInfo
- Publication number
- CA1251386C CA1251386C CA 462886 CA462886A CA1251386C CA 1251386 C CA1251386 C CA 1251386C CA 462886 CA462886 CA 462886 CA 462886 A CA462886 A CA 462886A CA 1251386 C CA1251386 C CA 1251386C
- Authority
- CA
- Canada
- Prior art keywords
- dna
- plant
- gene
- cells
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
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- 238000006482 condensation reaction Methods 0.000 description 1
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- 230000002596 correlated effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
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- 230000021121 meiosis Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000001035 methylating effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000037230 mobility Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- SCOAVUHOIJMIBW-UHFFFAOYSA-N phenanthrene-1,2-dione Chemical compound C1=CC=C2C(C=CC(C3=O)=O)=C3C=CC2=C1 SCOAVUHOIJMIBW-UHFFFAOYSA-N 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000004293 potassium hydrogen sulphite Substances 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 239000011369 resultant mixture Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- 231100000041 toxicology testing Toxicity 0.000 description 1
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- 230000002103 transcriptional effect Effects 0.000 description 1
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- 230000001018 virulence Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000560626A CA1251386A (fr) | 1983-09-14 | 1988-03-04 | Selection a l'aide de genes codant pour une opine syntethase |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53228083A | 1983-09-14 | 1983-09-14 | |
| US532,280 | 1983-09-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1251386C true CA1251386C (fr) | 1989-03-21 |
Family
ID=24121117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 462886 Expired CA1251386C (fr) | 1983-09-14 | 1984-09-11 | Selection a l'aide de genes codant pour une opine syntethase |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0140556B1 (fr) |
| JP (1) | JPS60156333A (fr) |
| AT (1) | ATE78295T1 (fr) |
| AU (2) | AU566953B2 (fr) |
| BR (1) | BR8404582A (fr) |
| CA (1) | CA1251386C (fr) |
| DE (1) | DE3485814D1 (fr) |
| ES (1) | ES8602343A1 (fr) |
| NZ (1) | NZ209338A (fr) |
| PT (1) | PT79191B (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR8404834A (pt) * | 1983-09-26 | 1985-08-13 | Agrigenetics Res Ass | Metodo para modificar geneticamente uma celula vegetal |
| US4771002A (en) * | 1984-02-24 | 1988-09-13 | Lubrizol Genetics, Inc. | Transcription in plants and bacteria |
| US5668298A (en) * | 1984-12-24 | 1997-09-16 | Eli Lilly And Company | Selectable marker for development of vectors and transformation systems in plants |
| CA1340766C (fr) | 1984-12-24 | 1999-09-28 | Clive Waldron | Marqueur selectionnable pour la conception de vecteurs et de systemes de transformation chez les vegetaux |
| EP0223417A1 (fr) * | 1985-10-21 | 1987-05-27 | Lubrizol Genetics Inc. | Plasmides sous-T-ADN à base de TL-ADN |
| ES2068811T5 (es) | 1986-04-02 | 2001-08-16 | Pioneer Hi Bred Int | Plantas resistentes a virus que tienen una proteina de cubierta viral. |
| US5597945A (en) * | 1986-07-25 | 1997-01-28 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Plants genetically enhanced for disease resistance |
| JPH02502513A (ja) * | 1986-07-25 | 1990-08-16 | ザ ボード オブ スーパーバイザーズ オブ ルイジアナ ステート ユニバーシティ アンド アグリカルチュラル アンド メカニカル カレッジ | 病気抵抗体及び害虫抵抗体を植物に組み込む方法並びに植物に組み込まれ該抵抗体の暗号に対応する新規な遺伝子 |
| EP0265556A1 (fr) * | 1986-10-31 | 1988-05-04 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Vecteurs binaires stables pour l'agrobactérium et leur utilisation |
| US5350689A (en) * | 1987-05-20 | 1994-09-27 | Ciba-Geigy Corporation | Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells |
| CA1327311C (fr) | 1987-07-06 | 1994-03-01 | Jesse M. Jaynes | Polypeptides antimicrobiens therapeutiques, leurs methodes de preparation et leurs usages |
| FI891054A (fi) * | 1988-03-08 | 1989-09-09 | Ciba Geigy Ag | Kemiskt reglerbara dna-sekvenser och gener samt olika anvaendningar daerav. |
| AU638438B2 (en) | 1989-02-24 | 1993-07-01 | Monsanto Technology Llc | Synthetic plant genes and method for preparation |
| US20010003849A1 (en) | 1989-08-07 | 2001-06-14 | Kenneth A. Barton | Expression of genes in plants |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3381568D1 (de) * | 1983-01-13 | 1990-06-21 | Max Planck Gesellschaft | Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren. |
| WO1984002919A1 (fr) * | 1983-01-17 | 1984-08-02 | Monsanto Co | Plasmides de transformation de cellules vegetales |
| JPS60500795A (ja) * | 1983-01-17 | 1985-05-30 | モンサント カンパニ− | 遺伝子的に形質転換された植物 |
-
1984
- 1984-08-24 NZ NZ209338A patent/NZ209338A/en unknown
- 1984-09-11 CA CA 462886 patent/CA1251386C/fr not_active Expired
- 1984-09-11 PT PT79191A patent/PT79191B/pt not_active IP Right Cessation
- 1984-09-12 AT AT84306233T patent/ATE78295T1/de not_active IP Right Cessation
- 1984-09-12 DE DE8484306233T patent/DE3485814D1/de not_active Expired - Lifetime
- 1984-09-12 EP EP84306233A patent/EP0140556B1/fr not_active Expired - Lifetime
- 1984-09-13 AU AU33018/84A patent/AU566953B2/en not_active Ceased
- 1984-09-13 ES ES535897A patent/ES8602343A1/es not_active Expired
- 1984-09-13 BR BR8404582A patent/BR8404582A/pt unknown
- 1984-09-14 JP JP59193841A patent/JPS60156333A/ja active Pending
-
1987
- 1987-10-27 AU AU80197/87A patent/AU8019787A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| BR8404582A (pt) | 1985-08-06 |
| AU566953B2 (en) | 1987-11-05 |
| ES535897A0 (es) | 1985-12-01 |
| DE3485814D1 (de) | 1992-08-20 |
| EP0140556B1 (fr) | 1992-07-15 |
| AU8019787A (en) | 1988-02-25 |
| PT79191B (en) | 1986-09-15 |
| AU3301884A (en) | 1985-03-21 |
| ES8602343A1 (es) | 1985-12-01 |
| EP0140556A1 (fr) | 1985-05-08 |
| PT79191A (en) | 1984-10-01 |
| JPS60156333A (ja) | 1985-08-16 |
| ATE78295T1 (de) | 1992-08-15 |
| NZ209338A (en) | 1988-02-12 |
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