CA1231783A - Spot quantitation - Google Patents
Spot quantitationInfo
- Publication number
- CA1231783A CA1231783A CA000472805A CA472805A CA1231783A CA 1231783 A CA1231783 A CA 1231783A CA 000472805 A CA000472805 A CA 000472805A CA 472805 A CA472805 A CA 472805A CA 1231783 A CA1231783 A CA 1231783A
- Authority
- CA
- Canada
- Prior art keywords
- model
- spot
- pixel
- image
- intensity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 claims abstract description 29
- 230000006870 function Effects 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 5
- 238000013178 mathematical model Methods 0.000 claims description 4
- 230000006872 improvement Effects 0.000 claims description 2
- 230000002093 peripheral effect Effects 0.000 abstract description 6
- 230000004907 flux Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 238000001962 electrophoresis Methods 0.000 abstract description 2
- 239000000499 gel Substances 0.000 description 56
- 150000002500 ions Chemical class 0.000 description 19
- 230000003287 optical effect Effects 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 3
- 241001237728 Precis Species 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- GJJFMKBJSRMPLA-HIFRSBDPSA-N (1R,2S)-2-(aminomethyl)-N,N-diethyl-1-phenyl-1-cyclopropanecarboxamide Chemical compound C=1C=CC=CC=1[C@@]1(C(=O)N(CC)CC)C[C@@H]1CN GJJFMKBJSRMPLA-HIFRSBDPSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004422 calculation algorithm Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002674 ointment Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 241001136792 Alle Species 0.000 description 1
- XUKUURHRXDUEBC-KAYWLYCHSA-N Atorvastatin Chemical compound C=1C=CC=CC=1C1=C(C=2C=CC(F)=CC=2)N(CC[C@@H](O)C[C@@H](O)CC(O)=O)C(C(C)C)=C1C(=O)NC1=CC=CC=C1 XUKUURHRXDUEBC-KAYWLYCHSA-N 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 101100072702 Drosophila melanogaster defl gene Proteins 0.000 description 1
- 101150039033 Eci2 gene Proteins 0.000 description 1
- 241000448280 Elates Species 0.000 description 1
- 241000490229 Eucephalus Species 0.000 description 1
- 101000657326 Homo sapiens Protein TANC2 Proteins 0.000 description 1
- 229940124913 IPOL Drugs 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- 125000000510 L-tryptophano group Chemical group [H]C1=C([H])C([H])=C2N([H])C([H])=C(C([H])([H])[C@@]([H])(C(O[H])=O)N([H])[*])C2=C1[H] 0.000 description 1
- 241001296096 Probles Species 0.000 description 1
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 1
- 101710171134 Protein Bet Proteins 0.000 description 1
- 102100034784 Protein TANC2 Human genes 0.000 description 1
- 241000366880 Quadrans Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 101100502015 Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) exp7 gene Proteins 0.000 description 1
- 244000166490 Tetrameles nudiflora Species 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 240000004543 Vicia ervilia Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- HUMHYXGDUOGHTG-HEZXSMHISA-N alpha-D-GalpNAc-(1->3)-[alpha-L-Fucp-(1->2)]-D-Galp Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](O)[C@@H](CO)OC1O HUMHYXGDUOGHTG-HEZXSMHISA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000000211 autoradiogram Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000002844 continuous effect Effects 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- PHTXVQQRWJXYPP-UHFFFAOYSA-N ethyltrifluoromethylaminoindane Chemical compound C1=C(C(F)(F)F)C=C2CC(NCC)CC2=C1 PHTXVQQRWJXYPP-UHFFFAOYSA-N 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000063 preceeding effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- NQLVQOSNDJXLKG-UHFFFAOYSA-N prosulfocarb Chemical compound CCCN(CCC)C(=O)SCC1=CC=CC=C1 NQLVQOSNDJXLKG-UHFFFAOYSA-N 0.000 description 1
- QHGVXILFMXYDRS-UHFFFAOYSA-N pyraclofos Chemical compound C1=C(OP(=O)(OCC)SCCC)C=NN1C1=CC=C(Cl)C=C1 QHGVXILFMXYDRS-UHFFFAOYSA-N 0.000 description 1
- 210000002320 radius Anatomy 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229920003987 resole Polymers 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000002226 simultaneous effect Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 101150113425 thiL gene Proteins 0.000 description 1
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
- G01N21/5907—Densitometers
- G01N21/5911—Densitometers of the scanning type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
- G01N21/5907—Densitometers
- G01N2021/5915—Processing scan data in densitometry
- G01N2021/5923—Determining zones of density; quantitating spots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/95—Detectors specially adapted therefor; Signal analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Image Processing (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US574,713 | 1984-01-27 | ||
| US06/574,713 US4618937A (en) | 1984-01-27 | 1984-01-27 | Spot quantitation |
| US65701284A | 1984-10-02 | 1984-10-02 | |
| US657,012 | 1991-02-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1231783A true CA1231783A (en) | 1988-01-19 |
Family
ID=27076476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000472805A Expired CA1231783A (en) | 1984-01-27 | 1985-01-24 | Spot quantitation |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0152786A3 (instruction) |
| CA (1) | CA1231783A (instruction) |
| DK (1) | DK36885A (instruction) |
| GR (1) | GR850222B (instruction) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2814408B2 (ja) * | 1990-05-22 | 1998-10-22 | 日立ソフトウェアエンジニアリング 株式会社 | 蛍光パターン読み取り装置および蛍光パターン読み取り方法 |
| DE102009035587B3 (de) * | 2009-07-31 | 2011-03-24 | Siemens Aktiengesellschaft | Verfahren zur Filterung eines Chromatogramms |
| CN112528513B (zh) * | 2020-12-21 | 2024-02-23 | 北京机电工程研究所 | 一种快速宽灰度星斑灰度分配方法及装置 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3908078A (en) * | 1971-10-06 | 1975-09-23 | Object Recognition Systems | Method and apparatus for digital recognition of objects particularly biological materials |
| DD135648A1 (de) * | 1978-03-10 | 1979-05-16 | Klaus Friedel | Verfahren zur analyse bewegter bilder |
| JPS5661650A (en) * | 1979-10-24 | 1981-05-27 | Omron Tateisi Electronics Co | Analyzing device of cell |
| JPS5776662A (en) * | 1980-10-31 | 1982-05-13 | Toshiba Corp | Erasure system for picture information |
| US4481509A (en) * | 1980-12-18 | 1984-11-06 | Rca Corporation | Raster-scanned display system for digitally-encoded graphics |
| EP0055965B1 (en) * | 1981-01-05 | 1989-03-29 | Image Processing Technologies Inc. | Process and device for the binarization of a pattern |
| JPS59106079A (ja) * | 1982-12-10 | 1984-06-19 | Omron Tateisi Electronics Co | 図形照合装置の二値化装置 |
-
1985
- 1985-01-23 EP EP85100653A patent/EP0152786A3/en not_active Withdrawn
- 1985-01-24 CA CA000472805A patent/CA1231783A/en not_active Expired
- 1985-01-25 GR GR850222A patent/GR850222B/el not_active IP Right Cessation
- 1985-01-25 DK DK36885A patent/DK36885A/da not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| GR850222B (instruction) | 1985-05-27 |
| DK36885D0 (da) | 1985-01-25 |
| EP0152786A3 (en) | 1987-05-06 |
| EP0152786A2 (en) | 1985-08-28 |
| DK36885A (da) | 1985-07-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |