CA1061229A - Composition for detecting fibrinogen, fibrinogen split products and fibrin split products - Google Patents
Composition for detecting fibrinogen, fibrinogen split products and fibrin split productsInfo
- Publication number
- CA1061229A CA1061229A CA247,241A CA247241A CA1061229A CA 1061229 A CA1061229 A CA 1061229A CA 247241 A CA247241 A CA 247241A CA 1061229 A CA1061229 A CA 1061229A
- Authority
- CA
- Canada
- Prior art keywords
- dyed
- staphylococcus aureus
- cells
- fibrinogen
- suspension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000008946 Fibrinogen Human genes 0.000 title claims abstract description 57
- 108010049003 Fibrinogen Proteins 0.000 title claims abstract description 57
- 229940012952 fibrinogen Drugs 0.000 title claims abstract description 57
- 102000009123 Fibrin Human genes 0.000 title claims abstract description 35
- 108010073385 Fibrin Proteins 0.000 title claims abstract description 35
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 229950003499 fibrin Drugs 0.000 title claims abstract description 35
- 239000000203 mixture Substances 0.000 title claims abstract description 23
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims abstract description 72
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 58
- 239000000725 suspension Substances 0.000 claims abstract description 42
- 239000000872 buffer Substances 0.000 claims abstract description 41
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- 210000004369 blood Anatomy 0.000 claims abstract description 16
- 239000008280 blood Substances 0.000 claims abstract description 16
- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- 229920001515 polyalkylene glycol Polymers 0.000 claims abstract description 14
- 150000003839 salts Chemical class 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000011534 incubation Methods 0.000 claims description 12
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000005273 aeration Methods 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 8
- 229920001451 polypropylene glycol Polymers 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 210000004556 brain Anatomy 0.000 claims description 5
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims description 5
- 238000001802 infusion Methods 0.000 claims description 4
- BEIHVSJTPTXQGB-QIXACUJNSA-N n'-anilino-n-phenyliminobenzenecarboximidamide Chemical compound C=1C=CC=CC=1N\N=C(C=1C=CC=CC=1)\N=NC1=CC=CC=C1 BEIHVSJTPTXQGB-QIXACUJNSA-N 0.000 claims description 4
- -1 polybutylene Polymers 0.000 claims description 4
- 229920001748 polybutylene Polymers 0.000 claims description 4
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical class CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 claims description 3
- 150000004072 triols Chemical class 0.000 claims description 3
- 239000007900 aqueous suspension Substances 0.000 claims 1
- 230000003139 buffering effect Effects 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 31
- 210000002966 serum Anatomy 0.000 abstract description 22
- 238000013207 serial dilution Methods 0.000 abstract description 5
- 238000007865 diluting Methods 0.000 abstract description 2
- 238000012800 visualization Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 84
- 239000000047 product Substances 0.000 description 45
- 239000000975 dye Substances 0.000 description 20
- 150000002500 ions Chemical class 0.000 description 14
- 239000012153 distilled water Substances 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 8
- 239000000243 solution Substances 0.000 description 6
- 108010065152 Coagulase Proteins 0.000 description 5
- 241000191940 Staphylococcus Species 0.000 description 5
- 238000010998 test method Methods 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- LNOBZXNCABUBKK-UHFFFAOYSA-N 2,3,5-triphenyltetrazolium Chemical compound C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 LNOBZXNCABUBKK-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 206010053567 Coagulopathies Diseases 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000002518 antifoaming agent Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000035602 clotting Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 description 1
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000237074 Centris Species 0.000 description 1
- 241001495084 Phylo Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- NLWXVDTUBBMEAL-UHFFFAOYSA-N n'-amino-n-phenyliminomethanimidamide Chemical compound NN=CN=NC1=CC=CC=C1 NLWXVDTUBBMEAL-UHFFFAOYSA-N 0.000 description 1
- 229940012957 plasmin Drugs 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/745—Assays involving non-enzymic blood coagulation factors
- G01N2333/75—Fibrin; Fibrinogen
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/882—Staphylococcus
- Y10S435/883—Staphylococcus aureus
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Neurosurgery (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA324,526A CA1067379A (en) | 1975-03-07 | 1979-03-30 | Method for the determination of fibrin split products and fibrinogen split products in blood serum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/556,504 US3990947A (en) | 1975-03-07 | 1975-03-07 | Composition for detecting fibrinogen, fibrinogen split products and fibrin split products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1061229A true CA1061229A (en) | 1979-08-28 |
Family
ID=24221614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA247,241A Expired CA1061229A (en) | 1975-03-07 | 1976-03-05 | Composition for detecting fibrinogen, fibrinogen split products and fibrin split products |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US3990947A (OSRAM) |
| JP (1) | JPS51117095A (OSRAM) |
| AU (1) | AU507738B2 (OSRAM) |
| BE (1) | BE839145A (OSRAM) |
| CA (1) | CA1061229A (OSRAM) |
| DE (1) | DE2608733A1 (OSRAM) |
| DK (1) | DK95676A (OSRAM) |
| FR (1) | FR2303291A1 (OSRAM) |
| GB (1) | GB1490466A (OSRAM) |
| IE (1) | IE42640B1 (OSRAM) |
| IT (1) | IT1057927B (OSRAM) |
| LU (1) | LU74498A1 (OSRAM) |
| NL (1) | NL7602094A (OSRAM) |
| NO (1) | NO760768L (OSRAM) |
| SE (1) | SE7602866L (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2525804B2 (de) * | 1975-06-10 | 1980-04-03 | Behringwerke Ag, 3550 Marburg | Stabiler Clumping-Faktor, Verwendung desselben zum Nachweis von Fibrinogen- und Fibrinspaltprodukten und Herstellung desselben |
| DE2644622C3 (de) * | 1976-10-02 | 1979-11-29 | Behringwerke Ag, 3550 Marburg | Extraktion von Mikroorganismen und diese enthaltende diagnostische Mittel |
| US4567137A (en) * | 1983-01-20 | 1986-01-28 | Philpot Van B | Serum thrombin time in clinical medicine |
| US4461830A (en) * | 1983-01-20 | 1984-07-24 | Buren Philpot V Jun | Serum fibrinogen viscosity in clinical medicine |
| JPS62502262A (ja) * | 1985-03-27 | 1987-09-03 | ブロンコスタット・プロプライアタリー・リミテツド | アジユバントを含有しないワクチン |
| DD261844A1 (de) * | 1986-07-09 | 1988-11-09 | Adw Ddr | Verfahren zur praezipitation von fibrinmonomeren |
| IL80313A0 (en) * | 1986-09-19 | 1987-01-30 | Yissum Res Dev Co | Immunoassay reagents,kits and methods |
| US5424193A (en) * | 1993-02-25 | 1995-06-13 | Quidel Corporation | Assays employing dyed microorganism labels |
| CN110257475B (zh) * | 2019-06-28 | 2023-05-02 | 深圳市国赛生物技术有限公司 | 纤维蛋白原检测试剂及其制备方法及检测试剂制品 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3881993A (en) * | 1971-03-16 | 1975-05-06 | Miles Lab | Testing device for microorganisms |
| US3834991A (en) * | 1972-05-23 | 1974-09-10 | Warner Lambert Co | Colorimetric assay for lysozyme |
| US3790447A (en) * | 1972-07-05 | 1974-02-05 | Abbott Lab | Streptococci diagnostic method |
-
1975
- 1975-03-07 US US05/556,504 patent/US3990947A/en not_active Expired - Lifetime
-
1976
- 1976-02-17 GB GB6135/76A patent/GB1490466A/en not_active Expired
- 1976-02-19 IE IE323/76A patent/IE42640B1/en unknown
- 1976-02-27 SE SE7602866A patent/SE7602866L/xx not_active Application Discontinuation
- 1976-03-01 NL NL7602094A patent/NL7602094A/xx not_active Application Discontinuation
- 1976-03-02 FR FR7605819A patent/FR2303291A1/fr active Granted
- 1976-03-03 BE BE164818A patent/BE839145A/xx unknown
- 1976-03-03 DE DE19762608733 patent/DE2608733A1/de not_active Withdrawn
- 1976-03-03 IT IT48400/76A patent/IT1057927B/it active
- 1976-03-05 NO NO760768A patent/NO760768L/no unknown
- 1976-03-05 LU LU74498A patent/LU74498A1/xx unknown
- 1976-03-05 DK DK95676*#A patent/DK95676A/da unknown
- 1976-03-05 CA CA247,241A patent/CA1061229A/en not_active Expired
- 1976-03-05 AU AU11727/76A patent/AU507738B2/en not_active Expired
- 1976-03-05 JP JP51024038A patent/JPS51117095A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| NL7602094A (nl) | 1976-09-09 |
| IE42640L (en) | 1976-09-07 |
| AU1172776A (en) | 1977-09-08 |
| IT1057927B (it) | 1982-03-30 |
| NO760768L (OSRAM) | 1976-09-08 |
| JPS5523080B2 (OSRAM) | 1980-06-20 |
| SE7602866L (sv) | 1976-09-08 |
| BE839145A (fr) | 1976-07-01 |
| FR2303291A1 (fr) | 1976-10-01 |
| DE2608733A1 (de) | 1976-09-16 |
| JPS51117095A (en) | 1976-10-14 |
| GB1490466A (en) | 1977-11-02 |
| US3990947A (en) | 1976-11-09 |
| LU74498A1 (OSRAM) | 1976-09-01 |
| FR2303291B1 (OSRAM) | 1980-08-01 |
| IE42640B1 (en) | 1980-09-24 |
| AU507738B2 (en) | 1980-02-28 |
| DK95676A (da) | 1976-09-08 |
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