CA1039647A - Method and apparatus for surface immunological detection of biological particles by radioimmunoassay - Google Patents
Method and apparatus for surface immunological detection of biological particles by radioimmunoassayInfo
- Publication number
- CA1039647A CA1039647A CA213,939A CA213939A CA1039647A CA 1039647 A CA1039647 A CA 1039647A CA 213939 A CA213939 A CA 213939A CA 1039647 A CA1039647 A CA 1039647A
- Authority
- CA
- Canada
- Prior art keywords
- substrate
- biological particles
- particles
- surface area
- particle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002245 particle Substances 0.000 title claims abstract description 111
- 238000000034 method Methods 0.000 title claims description 30
- 238000001514 detection method Methods 0.000 title abstract description 19
- 238000003127 radioimmunoassay Methods 0.000 title description 12
- 230000001900 immune effect Effects 0.000 title description 11
- 239000000758 substrate Substances 0.000 claims abstract description 71
- 102000004169 proteins and genes Human genes 0.000 claims description 35
- 108090000623 proteins and genes Proteins 0.000 claims description 35
- 210000002966 serum Anatomy 0.000 claims description 15
- 210000004369 blood Anatomy 0.000 claims description 12
- 239000008280 blood Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims description 2
- 239000002356 single layer Substances 0.000 claims 1
- 230000005855 radiation Effects 0.000 abstract description 27
- 230000008105 immune reaction Effects 0.000 abstract description 8
- 241000894006 Bacteria Species 0.000 abstract description 3
- 241000700605 Viruses Species 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 56
- 239000000243 solution Substances 0.000 description 38
- 239000000427 antigen Substances 0.000 description 21
- 102000036639 antigens Human genes 0.000 description 18
- 108091007433 antigens Proteins 0.000 description 18
- 229940088597 hormone Drugs 0.000 description 16
- 239000005556 hormone Substances 0.000 description 16
- 208000006454 hepatitis Diseases 0.000 description 11
- 231100000283 hepatitis Toxicity 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 230000006870 function Effects 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000007654 immersion Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- 208000006379 syphilis Diseases 0.000 description 3
- 206010018612 Gonorrhoea Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000536 complexating effect Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 208000001786 gonorrhea Diseases 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000589970 Spirochaetales Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- -1 amino - Chemical class 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000005250 beta ray Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000005251 gamma ray Effects 0.000 description 1
- 101150049033 haao gene Proteins 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000005055 memory storage Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009600 syphilis test Methods 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Nanotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pathology (AREA)
- Composite Materials (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Materials Engineering (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US43088474A | 1974-01-04 | 1974-01-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1039647A true CA1039647A (en) | 1978-10-03 |
Family
ID=23709483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA213,939A Expired CA1039647A (en) | 1974-01-04 | 1974-11-18 | Method and apparatus for surface immunological detection of biological particles by radioimmunoassay |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS50100226A (OSRAM) |
| CA (1) | CA1039647A (OSRAM) |
| CH (1) | CH612009A5 (OSRAM) |
| DE (1) | DE2461230A1 (OSRAM) |
| FR (1) | FR2257089B1 (OSRAM) |
| NL (1) | NL181051C (OSRAM) |
-
1974
- 1974-11-18 CA CA213,939A patent/CA1039647A/en not_active Expired
- 1974-12-20 JP JP14586874A patent/JPS50100226A/ja active Pending
- 1974-12-23 DE DE19742461230 patent/DE2461230A1/de not_active Ceased
- 1974-12-23 CH CH1719074A patent/CH612009A5/xx not_active IP Right Cessation
-
1975
- 1975-01-02 FR FR7500009A patent/FR2257089B1/fr not_active Expired
- 1975-01-03 NL NL7500085A patent/NL181051C/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NL7500085A (nl) | 1975-07-08 |
| CH612009A5 (en) | 1979-06-29 |
| DE2461230A1 (de) | 1975-07-17 |
| FR2257089A1 (OSRAM) | 1975-08-01 |
| JPS50100226A (OSRAM) | 1975-08-08 |
| FR2257089B1 (OSRAM) | 1981-05-22 |
| NL181051B (nl) | 1987-01-02 |
| NL181051C (nl) | 1987-06-01 |
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